##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032998_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3906515
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86135340578495	34.0	31.0	34.0	31.0	34.0
2	32.91382293425214	34.0	31.0	34.0	31.0	34.0
3	32.991346251070325	34.0	33.0	34.0	31.0	34.0
4	36.38473908330059	37.0	37.0	37.0	35.0	37.0
5	36.36877472632256	37.0	37.0	37.0	35.0	37.0
6	36.422774262994	37.0	37.0	37.0	35.0	37.0
7	36.38958944225224	37.0	37.0	37.0	35.0	37.0
8	36.332348397484715	37.0	37.0	37.0	35.0	37.0
9	38.18190407562751	39.0	39.0	39.0	37.0	39.0
10-11	38.19130810965784	39.0	39.0	39.0	37.0	39.0
12-13	38.18190420361883	39.0	39.0	39.0	37.0	39.0
14-15	39.73906934953533	41.0	40.0	41.0	37.0	41.0
16-17	39.754400277485175	41.0	40.0	41.0	37.0	41.0
18-19	39.77804360152207	41.0	40.0	41.0	37.0	41.0
20-21	39.78563707038114	41.0	40.0	41.0	37.0	41.0
22-23	39.75077441146392	41.0	40.0	41.0	37.0	41.0
24-25	39.73391283535325	41.0	40.0	41.0	37.0	41.0
26-27	39.65732103934069	41.0	40.0	41.0	37.0	41.0
28-29	39.59638232542304	41.0	40.0	41.0	37.0	41.0
30-31	39.48499237811707	41.0	40.0	41.0	36.5	41.0
32-33	39.36348471719678	41.0	39.0	41.0	35.5	41.0
34-35	39.27430139139361	41.0	39.0	41.0	35.0	41.0
36-37	39.19056384014908	41.0	39.0	41.0	35.0	41.0
38-39	39.096340728244996	41.0	39.0	41.0	35.0	41.0
40-41	38.99576988697086	41.0	39.0	41.0	35.0	41.0
42-43	38.85688638082793	41.0	39.0	41.0	35.0	41.0
44-45	38.68314367153333	41.0	39.0	41.0	35.0	41.0
46-47	38.458120857081056	41.0	38.5	41.0	35.0	41.0
48-49	38.25334716492833	41.0	38.0	41.0	34.5	41.0
50-51	38.093908125272776	40.5	38.0	41.0	34.0	41.0
52-53	37.951350372390735	40.0	38.0	41.0	34.0	41.0
54-55	37.7997263545641	40.0	37.0	41.0	34.0	41.0
56-57	37.58805751417823	40.0	37.0	41.0	33.0	41.0
58-59	37.4185706441675	40.0	36.5	41.0	33.0	41.0
60-61	37.227125199826446	40.0	36.0	41.0	33.0	41.0
62-63	36.987545548909964	39.0	35.5	41.0	33.0	41.0
64-65	36.70012709537785	39.0	35.0	41.0	32.5	41.0
66-67	36.42310128080911	38.5	35.0	41.0	32.0	41.0
68-69	36.12933458594169	37.5	35.0	40.5	32.0	41.0
70-71	35.80652755717052	37.0	35.0	40.0	31.5	41.0
72-73	35.46338194016918	36.5	35.0	39.0	31.0	41.0
74-75	35.11447108228178	36.0	35.0	39.0	31.0	41.0
76-77	34.768046583719766	36.0	35.0	38.5	31.0	40.5
78-79	34.42658750830344	35.0	35.0	37.0	31.0	39.5
80-81	34.100602967094716	35.0	34.5	37.0	30.5	39.0
82-83	33.79913234686159	35.0	34.0	36.5	30.0	39.0
84-85	33.52600501981945	35.0	34.0	36.0	30.0	37.0
86-87	33.38136779200899	35.0	34.0	36.0	30.0	37.0
88-89	33.198353263714594	35.0	34.0	35.5	30.0	37.0
90-91	33.031078595628074	35.0	34.0	35.0	30.0	36.0
92-93	32.87363058890085	35.0	34.0	35.0	30.0	36.0
94-95	32.77920358682867	35.0	34.0	35.0	30.0	36.0
96-97	32.71787168358499	35.0	34.0	35.0	30.0	36.0
98-99	32.61612805787255	35.0	34.0	35.0	30.0	36.0
100	32.53718954106154	35.0	34.0	35.0	30.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2139758230245974
1101	2	0.24575031343010778
1101	3	0.19995181706545395
1101	4	0.1423142312530885
1101	5	0.1572182641708011
1101	6	0.15682373580107623
1101	7	0.13798712175043448
1101	8	0.05566985500070132
1101	9	0.15517918855007196
1101	10-11	0.19265747578634773
1101	12-13	0.23414418652117064
1101	14-15	0.3595646655276852
1101	16-17	0.2663527682448006
1101	18-19	0.2798744852652888
1101	20-21	0.2819458299062134
1101	22-23	0.279848694897197
1101	24-25	0.2729309201386201
1101	26-27	0.32253840618725604
1101	28-29	0.298037725087255
1101	30-31	0.297357502164445
1101	32-33	0.3637176687293433
1101	34-35	0.3296291428294609
1101	36-37	0.34158346552590046
1101	38-39	0.3056269354275969
1101	40-41	0.27285047074939683
1101	42-43	0.32726009714600934
1101	44-45	0.27107585134398704
1101	46-47	0.25341678251540856
1101	48-49	0.25782866428875195
1101	50-51	0.2544699185793178
1101	52-53	0.30798652588873665
1101	54-55	0.31516141283962185
1101	56-57	0.274633968783327
1101	58-59	0.24916112735249385
1101	60-61	0.24264782471605884
1101	62-63	0.24463879755422369
1101	64-65	0.2722771557291068
1101	66-67	0.3389183846460355
1101	68-69	0.31650797473810144
1101	70-71	0.3608084144021433
1101	72-73	0.33760934100316575
1101	74-75	0.34235118649937135
1101	76-77	0.35579046832890526
1101	78-79	0.320803576990059
1101	80-81	0.3632276952139648
1101	82-83	0.33198124317050315
1101	84-85	0.3436583633149155
1101	86-87	0.31395176819832926
1101	88-89	0.32592364328512957
1101	90-91	0.36709896844426737
1101	92-93	0.35833602078260895
1101	94-95	0.3712693729720371
1101	96-97	0.351680037831251
1101	98-99	0.3696113196274524
1101	100	0.3947099877838838
1102	1	0.11777939108096547
1102	2	0.11993128625618255
1102	3	0.13144729097213315
1102	4	0.07126118649573954
1102	5	0.10691781492592156
1102	6	0.07782645847539271
1102	7	0.09143199573540528
1102	8	-0.03126919486338409
1102	9	0.046593973474713835
1102	10-11	0.08986389472799772
1102	12-13	0.0919024335906613
1102	14-15	0.14177832346920383
1102	16-17	0.12874516906760647
1102	18-19	0.12319087333356293
1102	20-21	0.15645872588963528
1102	22-23	0.17051711397343183
1102	24-25	0.1487763564574891
1102	26-27	0.16227205006319423
1102	28-29	0.16933471075468276
1102	30-31	0.19187496367402446
1102	32-33	0.23816609745756523
1102	34-35	0.20369876312383894
1102	36-37	0.2326048627216153
1102	38-39	0.25684041754932707
1102	40-41	0.18598677739067426
1102	42-43	0.16221795466253042
1102	44-45	0.1590622227781111
1102	46-47	0.14553994753205046
1102	48-49	0.1825277266555716
1102	50-51	0.16704844960861465
1102	52-53	0.18559764869704765
1102	54-55	0.11930587592723896
1102	56-57	0.1752541495665838
1102	58-59	0.19542727220339629
1102	60-61	0.17235008150411346
1102	62-63	0.17954289618116093
1102	64-65	0.20498108316690633
1102	66-67	0.21150471541731974
1102	68-69	0.24804280912382382
1102	70-71	0.21451264066122633
1102	72-73	0.23755124088428659
1102	74-75	0.25820036351082365
1102	76-77	0.28132463850653977
1102	78-79	0.2751274775556496
1102	80-81	0.19841259902317887
1102	82-83	0.27585254887480204
1102	84-85	0.25209424010213866
1102	86-87	0.26305508728520266
1102	88-89	0.22703398503393402
1102	90-91	0.21821687116745636
1102	92-93	0.2541548381304466
1102	94-95	0.2861438716504878
1102	96-97	0.2914659573434122
1102	98-99	0.32369500550478847
1102	100	0.32296191221465875
1103	1	0.15229521510440946
1103	2	0.17074831929021883
1103	3	0.17544764886994813
1103	4	0.12161913612818864
1103	5	0.1509022470619641
1103	6	0.1059224732003301
1103	7	0.12208611617216292
1103	8	-7.322203228383728E-4
1103	9	0.08871104958776499
1103	10-11	0.12621007587887334
1103	12-13	0.15290602949639265
1103	14-15	0.20039295147139313
1103	16-17	0.20991772314178547
1103	18-19	0.2286308510029471
1103	20-21	0.2122425756471742
1103	22-23	0.2062056406193733
1103	24-25	0.16598510933906852
1103	26-27	0.20595301179957204
1103	28-29	0.2160998465330266
1103	30-31	0.234524015503915
1103	32-33	0.2477495714223963
1103	34-35	0.2282319154815653
1103	36-37	0.2068887993626447
1103	38-39	0.1703205557752625
1103	40-41	0.19437716652164028
1103	42-43	0.20452477534501412
1103	44-45	0.17097543532208448
1103	46-47	0.19712620176869677
1103	48-49	0.2258140335059693
1103	50-51	0.20709837220802996
1103	52-53	0.237448923500736
1103	54-55	0.2851793866871404
1103	56-57	0.2563250171336904
1103	58-59	0.2625794730849478
1103	60-61	0.23394008054027182
1103	62-63	0.24080987171318213
1103	64-65	0.26770490316641826
1103	66-67	0.26498205886207415
1103	68-69	0.23084974018117066
1103	70-71	0.2648664447591571
1103	72-73	0.24537381269789904
1103	74-75	0.21840794769713767
1103	76-77	0.18918211917539196
1103	78-79	0.21794442353346
1103	80-81	0.1924192443150048
1103	82-83	0.14395476808306995
1103	84-85	0.12150852098490361
1103	86-87	0.10935594047074915
1103	88-89	0.1624013363932093
1103	90-91	0.15839884631428447
1103	92-93	0.12245488570046348
1103	94-95	0.18712695061498152
1103	96-97	0.15985344114560007
1103	98-99	0.11671503085472779
1103	100	0.13225813518361207
1104	1	0.11145757458614014
1104	2	0.1200227719170357
1104	3	0.13740621099812955
1104	4	0.07828432610821778
1104	5	0.07100023651018716
1104	6	0.07611986427063044
1104	7	0.08222702520865255
1104	8	0.048288516654778846
1104	9	0.0607234366979128
1104	10-11	0.11183340794327279
1104	12-13	0.15436583346829025
1104	14-15	0.21468695557145878
1104	16-17	0.1636373415169743
1104	18-19	0.15965709791884564
1104	20-21	0.16748853910155503
1104	22-23	0.17930492595442615
1104	24-25	0.12967103400048785
1104	26-27	0.1604342527674376
1104	28-29	0.18688708671395204
1104	30-31	0.22467074873246418
1104	32-33	0.18940008939169672
1104	34-35	0.18932485275890087
1104	36-37	0.14069571665272207
1104	38-39	0.17652939709891768
1104	40-41	0.19207430623811916
1104	42-43	0.2522433764957839
1104	44-45	0.21083398503429152
1104	46-47	0.19018489981656472
1104	48-49	0.20293379913431409
1104	50-51	0.16919765967529088
1104	52-53	0.17783686619304717
1104	54-55	0.1561803883300854
1104	56-57	0.17354655112845307
1104	58-59	0.16046157313345333
1104	60-61	0.2126054794761032
1104	62-63	0.17550211212126499
1104	64-65	0.20255922373775803
1104	66-67	0.23171268612477292
1104	68-69	0.1758550157412202
1104	70-71	0.13295864708283034
1104	72-73	0.12140343259351027
1104	74-75	0.13906121177873132
1104	76-77	0.1465161153502308
1104	78-79	0.19695520640754438
1104	80-81	0.16424093054899913
1104	82-83	0.13338306401238498
1104	84-85	0.18030095980260086
1104	86-87	0.18861063086433916
1104	88-89	0.15530162822606286
1104	90-91	0.16940540480394617
1104	92-93	0.1341701553674426
1104	94-95	0.16678634937006365
1104	96-97	0.18176616420879554
1104	98-99	0.18888474167727765
1104	100	0.18969733093010177
1105	1	0.03942353642316476
1105	2	0.09596940722089897
1105	3	0.09261031720192392
1105	4	0.06522145655149814
1105	5	0.038087856411785026
1105	6	0.08768481560985464
1105	7	0.05143068432492015
1105	8	-0.010503023039113657
1105	9	0.06193583027395988
1105	10-11	0.053211979673690735
1105	12-13	0.05306137812115708
1105	14-15	0.10385008737950585
1105	16-17	0.13418209127674174
1105	18-19	0.10538597434640451
1105	20-21	0.10632090928802285
1105	22-23	0.13088305674853018
1105	24-25	0.1476395044535863
1105	26-27	0.09518968426814922
1105	28-29	0.12481854617214339
1105	30-31	0.14365834367917785
1105	32-33	0.11443122673683348
1105	34-35	0.15691949378488346
1105	36-37	0.08434924933174415
1105	38-39	0.08160687851590609
1105	40-41	0.09579731939295044
1105	42-43	0.08979718702919115
1105	44-45	0.08702740614648263
1105	46-47	0.10424806205558212
1105	48-49	0.10854083733703135
1105	50-51	0.07493019119333866
1105	52-53	0.08853605743473025
1105	54-55	0.11927453901007823
1105	56-57	0.11168122224032118
1105	58-59	0.12251126936148893
1105	60-61	0.16380922448419
1105	62-63	0.10140959288562357
1105	64-65	0.1040189344761373
1105	66-67	0.17635950698995373
1105	68-69	0.13698770434001517
1105	70-71	0.12978755759205995
1105	72-73	0.08196901018032321
1105	74-75	0.1272231757331781
1105	76-77	0.1316817740279106
1105	78-79	0.11940047819449973
1105	80-81	0.13969406299968057
1105	82-83	0.11040570681971218
1105	84-85	0.07497610808787414
1105	86-87	0.04606968159851732
1105	88-89	0.032885258971781184
1105	90-91	0.04128495022496281
1105	92-93	0.07379800939486358
1105	94-95	0.08768289611909097
1105	96-97	0.06833153750561394
1105	98-99	0.08365764347280447
1105	100	0.08425841001132994
1106	1	0.021956386162706565
1106	2	0.09088693800866565
1106	3	0.036601619434904364
1106	4	4.301577791636646E-4
1106	5	0.027801554914184123
1106	6	0.012758859100614472
1106	7	0.024806029308471977
1106	8	-0.006003817546677226
1106	9	0.057716360657025234
1106	10-11	0.051332354058345686
1106	12-13	0.04578692358208514
1106	14-15	0.07434486179798938
1106	16-17	0.05496811303846272
1106	18-19	0.046412292589700144
1106	20-21	0.04732271459376847
1106	22-23	0.06518111436258778
1106	24-25	0.09741700148078536
1106	26-27	0.09680615628927569
1106	28-29	0.07616174700423528
1106	30-31	0.08877285514594035
1106	32-33	0.13108548473021386
1106	34-35	0.14142665636909868
1106	36-37	0.15361307775271626
1106	38-39	0.20153582464223518
1106	40-41	0.1715145991874607
1106	42-43	0.16170258487417044
1106	44-45	0.17006029577962778
1106	46-47	0.2141780728124303
1106	48-49	0.14645087629818931
1106	50-51	0.13910716450883598
1106	52-53	0.10742021169512839
1106	54-55	0.11048761219225156
1106	56-57	0.14495861640931906
1106	58-59	0.10250486715897722
1106	60-61	0.12884085818976843
1106	62-63	0.15541610034957642
1106	64-65	0.11073848339962922
1106	66-67	0.03224434232549811
1106	68-69	0.11366082047270254
1106	70-71	0.2047616988461769
1106	72-73	0.14744144418043703
1106	74-75	0.08688689668174021
1106	76-77	0.10838234705387606
1106	78-79	0.09358685294252211
1106	80-81	0.13439806687560463
1106	82-83	0.17430102167637784
1106	84-85	0.16019623667024518
1106	86-87	0.1572974361077044
1106	88-89	0.1252707955695982
1106	90-91	0.1348776288353548
1106	92-93	0.10792861000028608
1106	94-95	0.14657823260482417
1106	96-97	0.16506661849924598
1106	98-99	0.17519496033472137
1106	100	0.18170130207654012
1107	1	0.03454449886500299
1107	2	0.03365158347540387
1107	3	0.040314572500022905
1107	4	-0.01722185238880769
1107	5	0.04594390189560471
1107	6	0.05891504211027154
1107	7	-0.004912099834704975
1107	8	0.09279694949458417
1107	9	0.09440419102569564
1107	10-11	0.09254971319025174
1107	12-13	0.0322497653875331
1107	14-15	0.05618907501778381
1107	16-17	0.059462531406566654
1107	18-19	0.06352259540707905
1107	20-21	0.11205379314136366
1107	22-23	0.08473616550519125
1107	24-25	0.10270074182226807
1107	26-27	0.11411690492808901
1107	28-29	0.13656100860384868
1107	30-31	0.11803267851648513
1107	32-33	0.1367380568947354
1107	34-35	0.13625595998836815
1107	36-37	0.17387007778702213
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2216	10-11	-0.18464093027749584
2216	12-13	-0.1888736270071405
2216	14-15	-0.32159411880002864
2216	16-17	-0.2899965107640057
2216	18-19	-0.30291680243771424
2216	20-21	-0.2670507911217044
2216	22-23	-0.2676936317002898
2216	24-25	-0.28479683426030533
2216	26-27	-0.2910926050103839
2216	28-29	-0.3041291946603195
2216	30-31	-0.32603135638279923
2216	32-33	-0.3994928125032189
2216	34-35	-0.4247570228527664
2216	36-37	-0.3990962119195842
2216	38-39	-0.3615411839550404
2216	40-41	-0.3457446092736305
2216	42-43	-0.3832442862573515
2216	44-45	-0.28930330699626694
2216	46-47	-0.29208332324189
2216	48-49	-0.32687940872904164
2216	50-51	-0.30015085994174484
2216	52-53	-0.3288393239316889
2216	54-55	-0.32709565316872613
2216	56-57	-0.3472343260196169
2216	58-59	-0.39700419709091506
2216	60-61	-0.4125670362367728
2216	62-63	-0.3785123701431843
2216	64-65	-0.3787331158830014
2216	66-67	-0.35622419829451957
2216	68-69	-0.38273970149512593
2216	70-71	-0.4858766729765378
2216	72-73	-0.4051268161027295
2216	74-75	-0.42235034114116843
2216	76-77	-0.39747748489374857
2216	78-79	-0.3948493454366968
2216	80-81	-0.4340858325654793
2216	82-83	-0.4152749599424439
2216	84-85	-0.42108254708181647
2216	86-87	-0.34250463611656556
2216	88-89	-0.3804833813147539
2216	90-91	-0.37858089841728315
2216	92-93	-0.4175301893913499
2216	94-95	-0.4255143159899575
2216	96-97	-0.3904813204502844
2216	98-99	-0.4094895381116146
2216	100	-0.4281868888504903
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6.0
3	6.0
4	13.0
5	34.0
6	104.0
7	220.0
8	455.0
9	853.0
10	1433.0
11	2319.0
12	3659.0
13	5775.0
14	6825.0
15	9290.0
16	11833.0
17	17686.0
18	21473.0
19	11977.0
20	8171.0
21	7580.0
22	7832.0
23	8316.0
24	9215.0
25	10922.0
26	13599.0
27	16947.0
28	22164.0
29	28201.0
30	35877.0
31	45598.0
32	58227.0
33	76858.0
34	107226.0
35	169659.0
36	326946.0
37	759666.0
38	1385883.0
39	711010.0
40	2657.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.12739552484665	19.721730662948424	13.692194783928235	23.4586790282767
2	15.5712217729704	20.19265351677143	40.12374263886169	24.112382071396482
3	18.286219669218816	25.664237388359428	30.107589638833637	25.94195330358811
4	12.33082364133447	16.860481042647354	38.54088662812652	32.26780868789165
5	12.676968145861736	38.07265542965124	35.530119846593905	13.72025657789312
6	30.63520941761697	38.0750111164272	17.6659419549706	13.623837510985231
7	27.342178292944467	32.817156924608106	22.31340687437108	17.52725790807635
8	25.650310909597057	35.97007946920049	19.959632250519977	18.41997737068247
9	25.508759067658595	16.37508172348102	20.94588817616741	37.17027103269297
10-11	24.25055324246803	26.736464598241653	28.468455899951746	20.544526259338568
12-13	25.02825731557614	24.0332772688055	28.927248499652052	22.01121691596631
14-15	22.019648735150113	25.730069619668296	26.452107775966844	25.798173869214754
16-17	21.307149722962794	29.373610494264067	27.068077301635856	22.25116248113728
18-19	21.336164333872905	27.84555423567913	29.680247883629136	21.13803354681883
20-21	22.202448985860798	26.951861185737158	29.653860282118462	21.19182954628358
22-23	21.92854565656263	26.775200924303633	29.670830103147093	21.62542331598664
24-25	21.68844233969026	26.925943939587864	29.61302956610777	21.772584154614105
26-27	21.48009198015246	27.00906425618764	29.669154329153525	21.84168943450638
28-29	21.59610783704966	27.016504623345117	29.723810185759415	21.663577353845806
30-31	21.71610250853956	27.228222772609296	29.462756449283116	21.592918269568028
32-33	21.616079687709224	27.068661419661343	29.662874659452864	21.65238423317657
34-35	21.721594440671282	26.91357013141058	29.632563757719065	21.732271670199072
36-37	21.898219198322987	27.135298776785582	29.230373883589895	21.736108141301532
38-39	21.74041590665949	27.364458361636867	29.234661833351794	21.660463898351853
40-41	21.67457095431051	27.118681617581707	29.400351550204242	21.806395877903537
42-43	21.744012570438464	27.112808106641744	29.174473238891103	21.96870608402869
44-45	21.736064200876825	27.28808903166684	29.05054568490195	21.925301082554384
46-47	21.837148483245176	27.458725608927093	28.797679015870337	21.906446891957398
48-49	21.941199520152985	27.486272054600665	28.468325971014924	22.104202454231427
50-51	21.93016660236229	27.550198096342776	28.340200083777216	22.179435217517714
52-53	22.001854841127656	27.57165726902705	28.342167191453278	22.08432069839202
54-55	21.942865402402216	27.86070863015921	28.15816025662875	22.038265710809824
56-57	21.839547431947917	27.584525001489386	28.33968483972616	22.23624272683654
58-59	21.81900683896107	27.27226608098732	28.586688161617985	22.322038918433627
60-61	21.94991964159318	27.521431577490933	28.35501953391483	22.173629247001053
62-63	21.77248080080002	27.42889448530156	28.460807446366122	22.337817267532298
64-65	21.85028087694531	27.411350014015053	28.406892076441537	22.33147703259811
66-67	21.694779756936715	27.444139128150972	28.40535428842112	22.455726826491194
68-69	21.88341463776756	27.501624851433924	28.35671011805675	22.258250392741758
70-71	21.911371132582367	27.363942030172673	28.34302440922408	22.38166242802088
72-73	21.804664771541898	27.464901581076738	28.315032708181075	22.415400939200282
74-75	21.830147842770348	27.567153844283204	28.155913902800833	22.446784410145614
76-77	21.876833077286474	27.471005780989	28.092621767528396	22.559539374196135
78-79	21.846331537529366	27.527163604174977	28.155818717147817	22.470686141147837
80-81	21.776937526206307	27.478649697350725	28.17464319741805	22.569769579024918
82-83	21.745052549502663	27.40786078202343	28.297543048047785	22.549543620426125
84-85	21.73745653094894	27.42147412719521	28.08347081733975	22.757598524516094
86-87	21.8375638234094	27.42139858829504	28.129836512749833	22.61120107554572
88-89	21.612780849153758	27.420663657836002	28.120637880292364	22.845917612717876
90-91	21.5700095097792	27.335930710443222	28.200475872935016	22.89358390684256
92-93	21.697293449207873	27.361299527335	28.108355722492433	22.833051300964698
94-95	21.54950732343223	27.173130200110457	28.19921066795001	23.078151808507304
96-97	21.629286251485343	27.157454418300564	28.120374698387458	23.09288463182663
98-99	21.44668129266672	27.278031933077923	28.073575068920153	23.201711705335203
100	21.588013869139118	27.02290916584219	28.044791841321487	23.344285123697208
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	122.0
1	109.0
2	304.0
3	499.0
4	313.5
5	227.5
6	625.0
7	1504.5
8	2643.5
9	3126.0
10	2510.5
11	1881.5
12	1881.5
13	2133.5
14	2457.0
15	2779.0
16	3066.0
17	3454.0
18	3830.0
19	4341.0
20	5119.5
21	6235.0
22	8015.5
23	10955.5
24	14921.5
25	19315.0
26	25497.0
27	33490.5
28	43083.5
29	53562.0
30	62623.0
31	71738.0
32	80550.5
33	88607.0
34	97251.5
35	104975.5
36	112154.5
37	119225.5
38	124507.5
39	127311.5
40	130984.0
41	135027.0
42	136625.0
43	137080.5
44	136886.5
45	136964.0
46	136756.5
47	134425.5
48	132399.0
49	129400.5
50	124810.5
51	120499.5
52	112309.5
53	105727.0
54	102059.5
55	96521.0
56	88330.0
57	79493.5
58	72295.0
59	66675.0
60	60697.5
61	52924.0
62	46506.5
63	40474.0
64	33367.0
65	26522.5
66	21042.5
67	16185.0
68	12203.0
69	9454.5
70	7005.0
71	5018.0
72	3850.5
73	2892.5
74	2122.0
75	1481.5
76	926.5
77	647.0
78	454.5
79	268.0
80	156.5
81	72.0
82	36.0
83	23.0
84	10.0
85	5.0
86	3.5
87	2.0
88	1.5
89	2.0
90	2.0
91	1.5
92	0.5
93	0.0
94	0.5
95	0.5
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.071791609657201E-4
2	5.119652682762002E-4
3	1.2799131706905004E-4
4	0.011724004643524983
5	5.375635316900102E-4
6	0.003430167297450541
7	8.703409560695402E-4
8	0.13774425542971164
9	0.0012543149072766905
10-11	0.0
12-13	1.1519218536214504E-4
14-15	1.0239305365524002E-4
16-17	0.0
18-19	1.1519218536214504E-4
20-21	0.0
22-23	1.6638871218976504E-4
24-25	3.839739512071502E-4
26-27	0.0060795875607798765
28-29	0.007845867736332767
30-31	0.014770197989768375
32-33	0.017099639960425083
34-35	0.025790250389413585
36-37	0.037783036798783566
38-39	0.04281309555959723
40-41	0.053602763588518156
42-43	0.07047201917821895
44-45	0.10912539693307206
46-47	0.11645929940112863
48-49	0.1136050930304888
50-51	0.11517938623043812
52-53	0.10991894309890016
54-55	0.1161137228450422
56-57	0.0997692316553245
58-59	0.09171857781168126
60-61	0.09439359633842441
62-63	0.0926145170311646
64-65	0.0
66-67	3.8397395120715015E-5
68-69	1.0239305365524002E-4
70-71	0.0
72-73	0.0
74-75	0.0
76-77	8.959392194833503E-5
78-79	2.5598263413810005E-5
80-81	3.327774243795301E-4
82-83	2.0478610731048004E-4
84-85	0.0
86-87	8.447426926557302E-4
88-89	3.583756877933401E-4
90-91	2.559826341381001E-4
92-93	0.0012415157755697854
94-95	0.0012159175121559754
96-97	3.327774243795301E-4
98-99	3.8397395120715015E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3906515.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.5540174704599
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.55590459912673	35.16330416277895
2	16.11102938604531	16.28954522098455
3	5.916961591262209	8.973785389701298
4	2.80667783707529	5.675553616378274
5	1.532550075736182	3.87382816515608
6	0.9511394721935015	2.8850352896468574
7	0.6391354575282971	2.2617605560113145
8	0.45545318053787026	1.8419990436710418
9	0.31428691604170217	1.4299619619878259
>10	1.582746531261263	14.297829397255004
>50	0.0947766017283564	3.2631609347690236
>100	0.03827375578680803	3.1521894224118108
>500	7.604254958492781E-4	0.24605803470818594
>1k	2.0278012042627746E-4	0.21663664860649723
>5k	1.013900602131375E-4	0.4293521559333522
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9268	0.23724470531919115	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7485	0.1916030016523679	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4082	0.10449211125517245	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	2.559826341381001E-5	0.0
14-15	2.559826341381001E-5	0.0	0.0	5.119652682762002E-5	0.0
16-17	3.8397395120715015E-5	0.0	0.0	5.119652682762002E-5	0.0
18-19	5.119652682762002E-5	0.0	0.0	7.679479024143002E-5	0.0
20-21	5.119652682762002E-5	0.0	0.0	8.959392194833503E-5	0.0
22-23	6.399565853452502E-5	0.0	0.0	1.5358958048286006E-4	0.0
24-25	7.679479024143002E-5	1.2799131706905004E-5	0.0	3.0717916096572006E-4	0.0
26-27	7.679479024143002E-5	2.559826341381001E-5	0.0	5.759609268107251E-4	0.0
28-29	7.679479024143002E-5	2.559826341381001E-5	0.0	0.0018174767023805107	0.0
30-31	1.2799131706905004E-4	2.559826341381001E-5	0.0	0.005734011004693442	0.0
32-33	1.2799131706905004E-4	3.8397395120715015E-5	0.0	0.01398945095564717	0.0
34-35	1.2799131706905004E-4	5.119652682762002E-5	0.0	0.025956639101603347	0.0
36-37	1.2799131706905004E-4	5.119652682762002E-5	0.0	0.047459180369203754	0.0
38-39	1.2799131706905004E-4	5.119652682762002E-5	0.0	0.0861765537825914	0.0
40-41	2.0478610731048007E-4	5.119652682762002E-5	0.0	0.13394291331276087	0.0
42-43	2.0478610731048007E-4	5.119652682762002E-5	0.0	0.18298918601362082	0.0
44-45	2.0478610731048007E-4	5.119652682762002E-5	0.0	0.23901098549474403	0.0
46-47	2.0478610731048007E-4	5.119652682762002E-5	0.0	0.3097133890436873	0.0
48-49	2.3038437072429006E-4	5.119652682762002E-5	0.0	0.3914230458605688	0.0
50-51	2.3038437072429006E-4	5.119652682762002E-5	0.0	0.4821560905308184	0.0
52-53	2.3038437072429006E-4	5.119652682762002E-5	0.0	0.5718524055328087	0.0
54-55	2.3038437072429006E-4	5.119652682762002E-5	0.0	0.6721975981149438	0.0
56-57	2.559826341381001E-4	5.119652682762002E-5	0.0	0.7920486674184024	0.0
58-59	2.815808975519101E-4	5.119652682762002E-5	0.0	0.9251084406433867	0.0
60-61	3.071791609657201E-4	5.119652682762002E-5	0.0	1.0725672370386392	0.0
62-63	3.327774243795301E-4	5.119652682762002E-5	0.0	1.2220098988484622	0.0
64-65	3.327774243795301E-4	5.119652682762002E-5	0.0	1.3819094512628265	0.0
66-67	3.327774243795301E-4	5.119652682762002E-5	0.0	1.562492400515549	0.0
68-69	3.327774243795301E-4	5.119652682762002E-5	0.0	1.7680336565967365	0.0
70-71	3.583756877933401E-4	5.119652682762002E-5	0.0	1.9897658142871588	0.0
72-73	4.0957221462096014E-4	5.119652682762002E-5	0.0	2.221174115548001	0.0
74-75	4.0957221462096014E-4	5.119652682762002E-5	0.0	2.454143910877086	0.0
76-77	4.0957221462096014E-4	5.119652682762002E-5	0.0	2.703714180030027	0.0
78-79	4.0957221462096014E-4	5.119652682762002E-5	0.0	2.9901459484988537	0.0
80-81	4.0957221462096014E-4	5.119652682762002E-5	0.0	3.3046206145375097	0.0
82-83	4.0957221462096014E-4	5.119652682762002E-5	0.0	3.634403042097624	0.0
84-85	4.0957221462096014E-4	5.119652682762002E-5	0.0	3.9601665423017702	0.0
86-87	4.0957221462096014E-4	5.119652682762002E-5	0.0	4.293302342369094	0.0
88	4.0957221462096014E-4	5.119652682762002E-5	0.0	4.568675661043155	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6215	0.0	50.94843	1
GTATCAA	11135	0.0	41.431755	1
ATCAACG	13240	0.0	34.391426	3
TCAACGC	13330	0.0	34.15922	4
CAACGCA	13730	0.0	33.161503	5
TATCAAC	14165	0.0	32.444477	2
AACGCAG	14200	0.0	32.195988	6
ACGCAGA	16480	0.0	27.626463	7
CGCAGAG	16710	0.0	27.13362	8
GTACATG	12355	0.0	26.959728	1
TACATGG	12545	0.0	26.022654	2
ACACCGT	750	0.0	25.712624	6
GCAGAGT	18495	0.0	24.254925	9
ACATGGG	12745	0.0	23.805792	3
GAGTACT	11530	0.0	21.4901	12-13
TACACCG	725	0.0	20.76067	5
CATGGGG	8995	0.0	20.395119	4
CAGAGTA	18140	0.0	20.340153	10-11
AGAGTAC	16635	0.0	20.17552	10-11
GTGGTAT	3290	0.0	20.134241	1
>>END_MODULE