FastQCFastQC Report
Wed 25 May 2016
SRR1032997_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032997_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3357299
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48300.032.5013661
GGTATCA32950.031.8091241
TCAACGC47900.031.7920274
ATCAACG49300.030.8887523
CAACGCA49550.030.7329065
AACGCAG51750.029.608036
GTACATG100500.028.1535191
TACATGG102250.026.2467212
TATCAAC59100.026.1643962
ACGCAGA59800.025.307957
ACATGGG104900.024.3291323
CGCAGAG64350.023.372428
ACACCGT6050.020.1985516
CATGGGG70000.020.0762184
TACACCG4800.019.5835325
GCAGAGT77300.018.788039
GAGTACA72350.018.2545031
TAGACCG1350.00999412117.4075855
GTCTCGC7150.017.0910841
TATACTG21200.017.0709295
Produced by FastQC (version 0.11.5)