FastQCFastQC Report
Wed 25 May 2016
SRR1032997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032997_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3357299
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70110.20882858512155156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55950.16665182338540593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48850.051.4540021
GTATCAA89000.042.2836531
TCAACGC107750.034.539594
ATCAACG107900.034.4915733
CAACGCA109000.034.137895
AACGCAG114100.032.7356956
TATCAAC114900.032.5535662
ACGCAGA129550.028.7214477
GTACATG99950.028.4852331
CGCAGAG133900.027.612318
TACATGG102700.026.5710682
GCAGAGT148750.024.6938729
ACATGGG104600.024.24463
GTGGTAT25900.022.856031
GAGTACT101250.021.08491112-13
CAGAGTA145950.020.66159610-11
AGAGTAC138000.020.50741410-11
TGGTATC27250.020.2009052
CATGGGG71400.019.3734554
GAGTACA71850.018.8973561
Produced by FastQC (version 0.11.5)