FastQCFastQC Report
Wed 25 May 2016
SRR1032996_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032996_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072839
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10750.032.7929731
GTATCAA15200.029.377041
ACCGCGT650.00615329928.9237388
GTACATG34750.026.2406711
TCAACGC16000.025.8517974
ATCAACG16050.025.7712633
TACATGG35400.024.696632
CAACGCA17150.024.6652875
ATACCGT1351.4570645E-524.3709286
AACGCAG17500.023.9034046
ACATGGG36100.022.915723
TCTGTCG2504.3655746E-1022.5605138
TATCAAC18950.022.323452
GACCGCG1503.2905267E-521.9338347
GAGTACA26050.021.6521761
GTCTCGC2751.4351826E-920.5105131
CTATCGC1150.003959011820.4342969
CATACCG1656.857063E-519.939855
CATGGGG24600.019.680234
TATAACG1200.00506187919.5846942
Produced by FastQC (version 0.11.5)