##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032996_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1072839 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.466270335064255 33.0 31.0 34.0 30.0 34.0 2 31.495099451082595 34.0 31.0 34.0 28.0 34.0 3 31.63927299436355 34.0 31.0 34.0 29.0 34.0 4 34.91904470288645 37.0 35.0 37.0 32.0 37.0 5 35.01071549412354 37.0 35.0 37.0 33.0 37.0 6 35.0731256041214 37.0 36.0 37.0 33.0 37.0 7 34.983262167016676 37.0 35.0 37.0 33.0 37.0 8 34.9923595245885 37.0 36.0 37.0 33.0 37.0 9 36.58812738910498 39.0 38.0 39.0 33.0 39.0 10-11 36.577603442827865 39.0 38.0 39.0 33.0 39.0 12-13 36.514581405038406 39.0 38.0 39.0 33.0 39.0 14-15 37.967276543824376 41.0 39.0 41.0 33.0 41.0 16-17 37.98810911982133 41.0 39.0 41.0 33.0 41.0 18-19 38.011635483050114 41.0 39.0 41.0 33.0 41.0 20-21 38.05704397397932 41.0 39.0 41.0 33.0 41.0 22-23 38.04724427430398 41.0 39.0 41.0 33.0 41.0 24-25 38.08551562722832 41.0 39.0 41.0 33.0 41.0 26-27 38.068112270340656 41.0 39.0 41.0 33.0 41.0 28-29 38.0439749114266 41.0 39.0 41.0 33.0 41.0 30-31 38.00057697380502 41.0 39.0 41.0 33.0 41.0 32-33 37.94168649722838 41.0 38.5 41.0 33.0 41.0 34-35 37.863761477724054 41.0 38.0 41.0 32.0 41.0 36-37 37.7722076658287 41.0 38.0 41.0 32.0 41.0 38-39 37.673430030041786 40.5 38.0 41.0 32.0 41.0 40-41 37.58886981177977 40.0 38.0 41.0 31.5 41.0 42-43 37.47013624597913 40.0 38.0 41.0 31.0 41.0 44-45 37.35249837114423 40.0 38.0 41.0 31.0 41.0 46-47 37.23085290523555 40.0 38.0 41.0 31.0 41.0 48-49 37.09314911184251 40.0 37.0 41.0 30.5 41.0 50-51 36.957663731463896 40.0 37.0 41.0 30.0 41.0 52-53 36.789161281422466 40.0 36.5 41.0 30.0 41.0 54-55 36.61624903643511 40.0 36.0 41.0 30.0 41.0 56-57 36.43344434719469 40.0 35.5 41.0 30.0 41.0 58-59 36.21868565553639 39.0 35.0 41.0 30.0 41.0 60-61 35.9665308587775 39.0 35.0 41.0 29.5 41.0 62-63 35.7008675113414 39.0 35.0 41.0 29.0 41.0 64-65 35.40552589903984 38.0 35.0 40.0 29.0 41.0 66-67 35.10173753936984 37.5 35.0 40.0 29.0 41.0 68-69 34.771562182210005 37.0 34.0 40.0 28.0 41.0 70-71 34.42449100004754 36.5 34.0 39.0 28.0 41.0 72-73 34.13093204106114 36.0 34.0 39.0 28.0 41.0 74-75 33.865724493609946 35.5 34.0 39.0 28.0 40.5 76-77 33.54799275566977 35.0 34.0 37.5 27.5 40.0 78-79 33.184016893494736 35.0 34.0 37.0 27.0 39.0 80-81 32.871907154754815 35.0 34.0 37.0 26.5 39.0 82-83 32.57485466132383 35.0 33.0 36.0 26.0 38.5 84-85 32.4227232604333 35.0 34.0 36.0 26.0 37.0 86-87 32.20255602191941 35.0 33.0 36.0 26.0 37.0 88-89 31.982567281763618 35.0 33.0 35.0 26.0 37.0 90-91 31.797606630631435 35.0 33.0 35.0 25.0 36.0 92-93 31.611254344780534 35.0 33.0 35.0 25.0 36.0 94-95 31.43854203659636 35.0 33.0 35.0 25.0 36.0 96-97 31.272250542718897 35.0 33.0 35.0 24.5 36.0 98-99 31.099422187299307 35.0 33.0 35.0 24.0 35.0 100 30.96841650983978 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08176340713912822 1101 2 0.15574146682025258 1101 3 0.15899345246961616 1101 4 0.09333832886715498 1101 5 0.17427221683720973 1101 6 0.15561271842654634 1101 7 0.12316799221757435 1101 8 0.22595176089968305 1101 9 0.21863453609664418 1101 10-11 0.1156904540775372 1101 12-13 -0.08239363434195468 1101 14-15 -0.08734989080544153 1101 16-17 -0.05759738539416759 1101 18-19 -0.06885373730762723 1101 20-21 -0.05071556272793032 1101 22-23 0.03503653271206275 1101 24-25 -0.026274147416863514 1101 26-27 0.03166190435737093 1101 28-29 0.0826249104730934 1101 30-31 0.07955281482166043 1101 32-33 0.08500000802122543 1101 34-35 0.1227136645600595 1101 36-37 -0.013896735311952568 1101 38-39 -0.01336907918190633 1101 40-41 -0.02110347001553947 1101 42-43 -0.02505472763925809 1101 44-45 -0.09680602759690515 1101 46-47 -0.04833137040532165 1101 48-49 -0.1903679774760647 1101 50-51 -0.10392151777077174 1101 52-53 -0.20543980848616883 1101 54-55 -0.17198123933170706 1101 56-57 -0.16859662849191182 1101 58-59 -0.1511434052568177 1101 60-61 -0.12081861746970901 1101 62-63 -0.2034692022499911 1101 64-65 -0.17279670034993444 1101 66-67 -0.12440764778074964 1101 68-69 -0.2513603765940289 1101 70-71 -0.15559823014628904 1101 72-73 -0.3481398016588315 1101 74-75 -0.33536317165287954 1101 76-77 -0.3245753019453943 1101 78-79 -0.18939479993301234 1101 80-81 -0.1820164981198502 1101 82-83 -0.31761165642993916 1101 84-85 -0.27321950421975316 1101 86-87 -0.21575754051097817 1101 88-89 -0.17259604293844077 1101 90-91 -0.15853863130445234 1101 92-93 -0.10028159882866916 1101 94-95 -0.10912086160641365 1101 96-97 0.026436497234289646 1101 98-99 0.17050566535349176 1101 100 0.13106811792339812 1102 1 -0.028115072370479055 1102 2 0.03399597349660155 1102 3 -0.025045709385015158 1102 4 0.12435659002937882 1102 5 0.0675830120618528 1102 6 -0.08901604564559307 1102 7 -0.07329265495891946 1102 8 -0.11859626682672797 1102 9 -0.15861974746755436 1102 10-11 -0.07979951343884295 1102 12-13 -0.10823115575504971 1102 14-15 -0.07376746599234707 1102 16-17 -0.14987080366137917 1102 18-19 -0.12933036536455234 1102 20-21 -0.1566328561884731 1102 22-23 -0.07975490469741686 1102 24-25 -0.12109326585916591 1102 26-27 -0.1148022407638436 1102 28-29 -0.019246584491753538 1102 30-31 0.03791900047365715 1102 32-33 -0.03433497596385848 1102 34-35 0.10875710147036699 1102 36-37 0.12984373987911368 1102 38-39 0.048273451666183576 1102 40-41 0.12224277735153777 1102 42-43 0.21347744967061288 1102 44-45 0.16637318818279567 1102 46-47 0.1309465687190965 1102 48-49 0.20048669332006597 1102 50-51 0.1674778681035889 1102 52-53 0.060173315046121445 1102 54-55 0.032364673367155206 1102 56-57 0.14202677143472187 1102 58-59 0.1716088816427188 1102 60-61 0.1405596294738487 1102 62-63 0.1646561546349048 1102 64-65 0.15291134983895205 1102 66-67 0.08577581469292284 1102 68-69 0.14014543188498862 1102 70-71 0.1286180506119834 1102 72-73 0.24504071875971078 1102 74-75 0.047500822584019886 1102 76-77 -0.030364825884568347 1102 78-79 -0.1060182149859017 1102 80-81 -0.05682954715536681 1102 82-83 0.030083339807411846 1102 84-85 -0.04396160737319832 1102 86-87 -0.038101418729553416 1102 88-89 -7.668352890988217E-4 1102 90-91 0.12875300463702288 1102 92-93 -0.006289697043388998 1102 94-95 0.02030663828959689 1102 96-97 0.12941268095768876 1102 98-99 0.02769403629445577 1102 100 -0.018775325415571587 1103 1 -0.045135198801094845 1103 2 -0.06816378197943607 1103 3 -0.05850773246310581 1103 4 0.06966681030803556 1103 5 -0.09452841416207747 1103 6 -0.037365334086779 1103 7 0.02930413465553272 1103 8 -0.03571711281724532 1103 9 -0.1787918985764776 1103 10-11 0.004423688636329359 1103 12-13 0.0590782872751916 1103 14-15 -4.943223452755774E-4 1103 16-17 0.022393261778091755 1103 18-19 -0.08530892514207977 1103 20-21 -0.08282316909146914 1103 22-23 -0.05854126761862943 1103 24-25 0.002670095619173196 1103 26-27 0.009031046176630753 1103 28-29 0.02061765253404957 1103 30-31 0.10207897379439856 1103 32-33 0.1274651474814732 1103 34-35 0.0013166288818240446 1103 36-37 -0.004255815152895082 1103 38-39 0.07316153730873509 1103 40-41 0.10558438071549858 1103 42-43 0.09706231295960777 1103 44-45 0.0929265756007851 1103 46-47 0.13098354853080707 1103 48-49 0.040655912938461825 1103 50-51 -0.010493190823410714 1103 52-53 0.046829654259916254 1103 54-55 0.16875227020383932 1103 56-57 0.04823588352927999 1103 58-59 -0.031867489602319665 1103 60-61 -0.08162583705673399 1103 62-63 -0.047650323286795526 1103 64-65 -0.04681325141781656 1103 66-67 -0.006898955259032391 1103 68-69 0.13721999259843898 1103 70-71 0.12017925757925951 1103 72-73 0.07260990798742029 1103 74-75 0.04092917386264361 1103 76-77 0.05019065122896649 1103 78-79 0.02660147521741152 1103 80-81 0.09378586414754864 1103 82-83 0.12514229907448993 1103 84-85 0.04863920295532864 1103 86-87 0.012008915581390056 1103 88-89 0.09519174194290514 1103 90-91 0.0573907087151575 1103 92-93 0.11836992059880203 1103 94-95 0.2276073944258883 1103 96-97 0.11604952172531213 1103 98-99 0.12060009815966666 1103 100 0.1417587437487775 1104 1 0.17837225702238868 1104 2 0.20170319334861375 1104 3 0.20767250165928886 1104 4 0.1205386204470642 1104 5 0.17927382269190417 1104 6 0.1577522040478314 1104 7 0.134301617371392 1104 8 0.24645482645824757 1104 9 0.17075300126042237 1104 10-11 0.1455000339931729 1104 12-13 0.18808642599486802 1104 14-15 0.1816821938104738 1104 16-17 0.19498506351619227 1104 18-19 0.24885829866352083 1104 20-21 0.2348771239571903 1104 22-23 0.2088317357173608 1104 24-25 0.3226656727274104 1104 26-27 0.2693053937894021 1104 28-29 0.2714535747989473 1104 30-31 0.295970378541611 1104 32-33 0.39530909896311783 1104 34-35 0.41892957623444005 1104 36-37 0.46356401146461224 1104 38-39 0.48808588832208954 1104 40-41 0.3531414174122389 1104 42-43 0.3720526138231861 1104 44-45 0.38555347816071617 1104 46-47 0.3173562511614847 1104 48-49 0.3922180620532174 1104 50-51 0.4956716957790377 1104 52-53 0.5232465302012201 1104 54-55 0.4536329098720415 1104 56-57 0.4495074435225561 1104 58-59 0.43780853999255953 1104 60-61 0.4642695782352675 1104 62-63 0.4034737285611314 1104 64-65 0.4589842015269312 1104 66-67 0.5975426717662913 1104 68-69 0.5341075857026638 1104 70-71 0.5558984715364303 1104 72-73 0.4755977980755688 1104 74-75 0.5371051262494078 1104 76-77 0.5171241885115094 1104 78-79 0.5690081956175064 1104 80-81 0.5898292153307167 1104 82-83 0.5069142104609554 1104 84-85 0.5058088003224626 1104 86-87 0.4064910800778847 1104 88-89 0.47831212202072493 1104 90-91 0.47505156359939704 1104 92-93 0.4654743166708961 1104 94-95 0.45728957500563183 1104 96-97 0.49308478979575554 1104 98-99 0.4902430630028043 1104 100 0.42481953883415 1105 1 0.1607719080039196 1105 2 0.10713458630151962 1105 3 0.23769297420844282 1105 4 0.1935771093361751 1105 5 0.2276757138156924 1105 6 0.2360777101287468 1105 7 0.16354184820704631 1105 8 0.2723100086291623 1105 9 0.28600774086665126 1105 10-11 0.22778904380227516 1105 12-13 0.2553844468714104 1105 14-15 0.30583086110794966 1105 16-17 0.2395416254524818 1105 18-19 0.16367887618311272 1105 20-21 0.25096272785599183 1105 22-23 0.24948336379414826 1105 24-25 0.2545830639633735 1105 26-27 0.2576814479435612 1105 28-29 0.20091227331437267 1105 30-31 0.29642858945771167 1105 32-33 0.33247521966754334 1105 34-35 0.2568040405167622 1105 36-37 0.2928512637637368 1105 38-39 0.20690809172469216 1105 40-41 0.09314790769874293 1105 42-43 0.17833887850601116 1105 44-45 0.18142486121262635 1105 46-47 0.13402014455521538 1105 48-49 0.16602412666303223 1105 50-51 0.039072796313078584 1105 52-53 0.0069225076143624165 1105 54-55 -0.03668733278519909 1105 56-57 0.19528668768563762 1105 58-59 0.15868606977626598 1105 60-61 0.1581650250178086 1105 62-63 0.10027697271096514 1105 64-65 -0.01462520905737108 1105 66-67 0.18121997372769982 1105 68-69 0.21657362966607963 1105 70-71 0.204921327444616 1105 72-73 0.15874701919979373 1105 74-75 0.12955631871538031 1105 76-77 0.18044057590544327 1105 78-79 0.12912985537582244 1105 80-81 0.09300337107607248 1105 82-83 0.09007142026691639 1105 84-85 0.053116283440687084 1105 86-87 -0.0012100505068417533 1105 88-89 0.06278084157163732 1105 90-91 0.11701954430072803 1105 92-93 0.05416813823272193 1105 94-95 0.08697278445230694 1105 96-97 0.003928286967429528 1105 98-99 -0.07852975958471475 1105 100 -0.08262814771812543 1106 1 -0.04555879920771844 1106 2 -0.07510746608917174 1106 3 -0.07877453100769216 1106 4 -0.07937328164565116 1106 5 0.008381060421697839 1106 6 0.06481790979018598 1106 7 -0.0811247597310043 1106 8 -0.0231362217473432 1106 9 -0.04999933196398132 1106 10-11 0.059550791312062756 1106 12-13 -0.04646300474295373 1106 14-15 0.02014707346111777 1106 16-17 -0.0687677600953549 1106 18-19 -0.06625736358202516 1106 20-21 -0.015362241410684874 1106 22-23 -0.07085110363647118 1106 24-25 -0.09768588454829796 1106 26-27 -0.011381941666080309 1106 28-29 -0.007703294690593054 1106 30-31 3.792002750486745E-4 1106 32-33 -0.049141571321257516 1106 34-35 0.0782702243389295 1106 36-37 0.14027311700091616 1106 38-39 0.011552692302608136 1106 40-41 0.043074615967498175 1106 42-43 0.13176966166803794 1106 44-45 0.09900440079611172 1106 46-47 0.1296627189957249 1106 48-49 0.23929641397614176 1106 50-51 0.23867570353851875 1106 52-53 0.1046876058300441 1106 54-55 0.25573695292794696 1106 56-57 0.1649035548629243 1106 58-59 0.12611311335370345 1106 60-61 0.19586502970022934 1106 62-63 0.12548127291580613 1106 64-65 0.14547119317971635 1106 66-67 0.21664662525190437 1106 68-69 0.15623116244498192 1106 70-71 0.24776663387707032 1106 72-73 0.14778401267535912 1106 74-75 0.16587858170372982 1106 76-77 0.13382671737952023 1106 78-79 0.1791888617669315 1106 80-81 0.09659831660527374 1106 82-83 0.17202641668367136 1106 84-85 0.14395319009313567 1106 86-87 0.12857960579580663 1106 88-89 0.15452347847083558 1106 90-91 0.22030771215029787 1106 92-93 0.19635647774446596 1106 94-95 0.19724410274317705 1106 96-97 0.19320507574994394 1106 98-99 0.15138227446518826 1106 100 0.21120542648191432 1107 1 0.20154548203170108 1107 2 0.2303271021793698 1107 3 0.29987743037016656 1107 4 0.30236226993529414 1107 5 0.2792445925278173 1107 6 0.2278195938102172 1107 7 0.26604705912978943 1107 8 0.24920222130483438 1107 9 0.25945712664299236 1107 10-11 0.24349125211314515 1107 12-13 0.2917622895377221 1107 14-15 0.32195029335662184 1107 16-17 0.4148550394063264 1107 18-19 0.33753054752969547 1107 20-21 0.34960672088164557 1107 22-23 0.277631683111089 1107 24-25 0.4256518808213201 1107 26-27 0.42630585770297813 1107 28-29 0.40736401421772683 1107 30-31 0.42151874484007834 1107 32-33 0.39726980544296 1107 34-35 0.473580760938205 1107 36-37 0.4470455605750985 1107 38-39 0.44339120319992276 1107 40-41 0.5259345901440611 1107 42-43 0.5418191213478494 1107 44-45 0.5172977170317026 1107 46-47 0.5094878438365456 1107 48-49 0.6134994258388531 1107 50-51 0.5925947498780957 1107 52-53 0.4971384603904525 1107 54-55 0.5574156299132298 1107 56-57 0.7039095102435127 1107 58-59 0.7188305051176442 1107 60-61 0.699086232131279 1107 62-63 0.6201572556996737 1107 64-65 0.5600532075690339 1107 66-67 0.5051964037846304 1107 68-69 0.4281592673593124 1107 70-71 0.6410177919531534 1107 72-73 0.46048512160697186 1107 74-75 0.5616211745717976 1107 76-77 0.5124796397366183 1107 78-79 0.6291672012947345 1107 80-81 0.5333380138506882 1107 82-83 0.4301246848837579 1107 84-85 0.45633083341069636 1107 86-87 0.4550911396609081 1107 88-89 0.4083968975158285 1107 90-91 0.5034856167004556 1107 92-93 0.5532251537802928 1107 94-95 0.5447695619663335 1107 96-97 0.5019582897450299 1107 98-99 0.5635382006512195 1107 100 0.5885573751364106 1108 1 -0.01234436486057433 1108 2 0.0070807555534564415 1108 3 0.0233232671128647 1108 4 -0.03319926656688921 1108 5 -0.05773817017361438 1108 6 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88-89 -0.3582483999276995 2214 90-91 -0.2934082749464366 2214 92-93 -0.2813600161754941 2214 94-95 -0.3418503218336042 2214 96-97 -0.3770919072811729 2214 98-99 -0.5609950440588314 2214 100 -0.5298025566525055 2215 1 -0.4027622069639456 2215 2 -0.4884149731057761 2215 3 -0.4789514356797113 2215 4 -0.4351664252494203 2215 5 -0.46497909172019547 2215 6 -0.4655912541172569 2215 7 -0.4790155961787903 2215 8 -0.5475584639413995 2215 9 -0.5698265092106425 2215 10-11 -0.47688000999376357 2215 12-13 -0.5460375357663452 2215 14-15 -0.5711692258274823 2215 16-17 -0.6294793573624062 2215 18-19 -0.5603826623387533 2215 20-21 -0.5969478149283276 2215 22-23 -0.5965242407685949 2215 24-25 -0.5411346795587946 2215 26-27 -0.5980124308213846 2215 28-29 -0.686412213521443 2215 30-31 -0.6530436209443451 2215 32-33 -0.6504532429283145 2215 34-35 -0.6541044609189797 2215 36-37 -0.6827437373834186 2215 38-39 -0.6291769442031807 2215 40-41 -0.6551109324256785 2215 42-43 -0.6924964729713352 2215 44-45 -0.5900470472031785 2215 46-47 -0.6507747825987664 2215 48-49 -0.6442899455691986 2215 50-51 -0.5955063610145714 2215 52-53 -0.6599230957077467 2215 54-55 -0.6173835156659777 2215 56-57 -0.59141542641418 2215 58-59 -0.593949936788988 2215 60-61 -0.6366099919607393 2215 62-63 -0.5666781169665214 2215 64-65 -0.6361800656433161 2215 66-67 -0.6630549684079483 2215 68-69 -0.5288990278324874 2215 70-71 -0.5933999035611279 2215 72-73 -0.627044887438089 2215 74-75 -0.5980731676922986 2215 76-77 -0.7839247202535091 2215 78-79 -0.6192247444564458 2215 80-81 -0.5869443173238054 2215 82-83 -0.7220686229131985 2215 84-85 -0.60120849296203 2215 86-87 -0.5766574248279426 2215 88-89 -0.5136777291183066 2215 90-91 -0.5337938249310561 2215 92-93 -0.5742894245291055 2215 94-95 -0.5440418566514182 2215 96-97 -0.4160172509243303 2215 98-99 -0.37392901220144026 2215 100 -0.37773276765013364 2216 1 -0.07985859397751227 2216 2 -0.08213453072425381 2216 3 -0.29089391145739896 2216 4 -0.2507516799937335 2216 5 -0.10805961375591266 2216 6 -0.2358410368962396 2216 7 -0.30239150768381506 2216 8 -0.2847844290289032 2216 9 -0.35440808228234033 2216 10-11 -0.44009582487659316 2216 12-13 -0.4700450541675707 2216 14-15 -0.5744689628286466 2216 16-17 -0.5061413801276373 2216 18-19 -0.5299005444707774 2216 20-21 -0.5145216759084335 2216 22-23 -0.5444880416735742 2216 24-25 -0.5804229673074772 2216 26-27 -0.5432851802988452 2216 28-29 -0.6105077612101084 2216 30-31 -0.6399199969691622 2216 32-33 -0.6758380890819069 2216 34-35 -0.7205504968914553 2216 36-37 -0.6548018932385631 2216 38-39 -0.6666421570771774 2216 40-41 -0.5843932072165572 2216 42-43 -0.6249475428188873 2216 44-45 -0.6165447702394502 2216 46-47 -0.6478610002201393 2216 48-49 -0.6572271393303524 2216 50-51 -0.6007948598261095 2216 52-53 -0.5984522053984662 2216 54-55 -0.5686353854780535 2216 56-57 -0.6876612560428939 2216 58-59 -0.6761373984625436 2216 60-61 -0.7167644827119659 2216 62-63 -0.6940848882467279 2216 64-65 -0.6441402987044214 2216 66-67 -0.6430401680788549 2216 68-69 -0.7116580705405724 2216 70-71 -0.7681080910108804 2216 72-73 -0.7051426821423803 2216 74-75 -0.7349422446760556 2216 76-77 -0.7485318905306784 2216 78-79 -0.6989884837020313 2216 80-81 -0.7134822045327454 2216 82-83 -0.8217315187111538 2216 84-85 -0.8399839809411702 2216 86-87 -0.8268657003069819 2216 88-89 -0.8513299426465011 2216 90-91 -0.9216551436694225 2216 92-93 -0.9839961379096316 2216 94-95 -0.9847783104479717 2216 96-97 -0.9690007696118208 2216 98-99 -1.0080322323101818 2216 100 -1.0001306854398528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17609.0 3 2776.0 4 1759.0 5 1589.0 6 1229.0 7 1317.0 8 1469.0 9 1620.0 10 1683.0 11 1849.0 12 2024.0 13 2271.0 14 2361.0 15 2483.0 16 2548.0 17 2513.0 18 2385.0 19 2484.0 20 2431.0 21 2542.0 22 2945.0 23 4169.0 24 6722.0 25 8947.0 26 9114.0 27 7637.0 28 7931.0 29 9873.0 30 11950.0 31 14070.0 32 18041.0 33 24146.0 34 34863.0 35 53647.0 36 102186.0 37 216721.0 38 334918.0 39 147656.0 40 361.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.05906068190065 19.017604868506847 12.45157565112268 23.471758798469825 2 16.32761383798225 19.596158976722144 39.84380684436468 24.232420340930926 3 18.61864383063275 24.890623979701303 29.37993824569259 27.11079394397336 4 12.604341190562371 16.540480837043035 38.26210581698639 32.5930721554082 5 12.796634281263264 37.60397766759796 34.721661217274495 14.87772683386428 6 31.13077125431672 37.62530387296944 17.753084438905443 13.4908404338084 7 28.039033509265842 32.12120872908819 21.696321462086825 18.14343629955914 8 25.417513603065668 36.26097363715146 20.10688422865277 18.2146285311301 9 24.897038150222155 17.543579798115484 20.913265777300165 36.64611627436219 10-11 24.00955776003642 26.991343818635766 28.164282279478194 20.834816141849622 12-13 24.7253211097321 24.306207554025118 28.28363530870796 22.684836027534818 14-15 22.11920999290139 26.50257779320007 25.257895920025447 26.120316293873092 16-17 21.826185994362223 29.812024394293573 25.618438996543087 22.74335061480112 18-19 21.697280739065867 28.479295857679077 27.75344758109651 22.069975822158543 20-21 23.014171794649364 28.012357528830034 27.059611457951476 21.913859218569126 22-23 22.59731537363693 27.962230534593246 27.177131236998964 22.263322854770855 24-25 22.378747619587315 27.847481951306424 27.28355210042998 22.490218328676285 26-27 22.196492484895632 27.614522205773845 27.470264663385073 22.718720645945446 28-29 22.304186538492033 27.71255663062144 27.434778593741694 22.54847823714484 30-31 22.560257231847274 28.454594045496904 26.859114818722258 22.12603390393357 32-33 21.994883598364805 27.984524867251064 27.564718749489874 22.455872784894257 34-35 22.077020802668475 27.361310879659428 27.76496771678075 22.796700600891356 36-37 22.739907210321405 28.07979091541663 26.964365330910677 22.215936543351287 38-39 22.448063016950496 28.20866669620392 27.20782022073655 22.135450066109033 40-41 22.024773672877263 27.81695707958142 27.61029993036806 22.54796931717325 42-43 21.943084592336653 27.909389074002906 27.532854109842052 22.614672223818392 44-45 22.242329997826495 28.031783552440714 27.385468856838752 22.340417592894035 46-47 22.148961704743634 28.5273376627314 27.105707370564662 22.217993261960302 48-49 21.97562052995238 27.933351773652394 27.606230464495468 22.484797231899762 50-51 22.011374931956116 27.759806365875768 27.626633927859256 22.602184774308867 52-53 22.076439722683624 27.86117202226106 27.511051467161856 22.55133678789346 54-55 22.260688987341418 28.488013575627306 27.198832749801525 22.05246468722975 56-57 21.806868404174683 28.148329543466854 27.345997317940647 22.69880473441782 58-59 21.755938471068173 27.4906222392628 27.879819133523824 22.873620156145197 60-61 22.343369495607867 28.09038656272753 27.243350698501406 22.322893243163193 62-63 22.059214509401876 28.075584416311322 27.255083412082648 22.610117662204154 64-65 22.035422500918866 27.658926429185783 27.636491183554824 22.669159886340527 66-67 21.872662040967107 27.790205432553904 27.33763253487465 22.999499991604335 68-69 22.501837707138513 28.058616327794716 27.174044858711106 22.265501106355668 70-71 22.558309938773807 27.605564694682926 27.446704014007352 22.38942135253592 72-73 22.116662321240923 27.84105723042308 27.758595197705986 22.283685250630008 74-75 22.111548396063878 27.811167525948697 27.553522838681033 22.523761239306392 76-77 22.20573200399994 27.85928568230325 27.258688303159655 22.676294010537156 78-79 22.437720029775733 27.97132313570532 27.344325727089387 22.246631107429568 80-81 22.073864842161626 27.717016395078907 27.582687848910236 22.626430913849227 82-83 21.94989307444211 27.814917339664134 27.662819523184307 22.57237006270945 84-85 22.117093292363833 27.77078162006759 27.49942398301519 22.61270110455338 86-87 22.43721446545134 27.819757883027325 27.412482713426506 22.330544938094825 88-89 21.899582198815157 27.77710927100736 27.53765947384475 22.785649056332733 90-91 21.913484234478982 27.645709867028334 27.54105257070314 22.899753327789544 92-93 22.28529294071228 27.953216046981698 27.247949540937828 22.513541471368196 94-95 22.046327112358124 27.610832108291234 27.56153504615362 22.781305733197023 96-97 22.02921234496274 27.56438179577849 27.648563867842075 22.757841991416694 98-99 22.035902953545172 27.681166922943106 27.553844869504225 22.729085254007497 100 22.37436491050683 27.59527391746798 27.37253204712651 22.65782912489868 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1474.0 1 970.5 2 430.5 3 334.0 4 234.0 5 193.5 6 174.5 7 154.0 8 144.0 9 145.0 10 149.0 11 147.5 12 150.5 13 158.0 14 162.5 15 176.0 16 235.5 17 312.5 18 424.0 19 571.5 20 805.0 21 1158.0 22 1679.5 23 2472.0 24 3507.0 25 4866.5 26 6777.5 27 9018.0 28 11711.5 29 14600.5 30 17044.0 31 19690.0 32 22100.5 33 24133.5 34 26231.5 35 28193.5 36 29847.0 37 31273.5 38 32839.5 39 33652.5 40 34102.0 41 34747.0 42 34992.5 43 35338.0 44 35404.0 45 35403.0 46 35818.0 47 35823.5 48 35827.0 49 35756.5 50 35239.5 51 34285.0 52 32300.0 53 30633.5 54 29653.0 55 28574.5 56 26960.5 57 24945.5 58 23029.0 59 21119.0 60 18972.0 61 16422.5 62 14149.5 63 12118.5 64 9869.0 65 7777.0 66 6217.5 67 4824.0 68 3632.5 69 2802.0 70 2131.0 71 1576.0 72 1209.0 73 950.5 74 736.5 75 536.0 76 373.5 77 267.5 78 195.0 79 129.0 80 91.5 81 66.0 82 50.5 83 49.5 84 39.0 85 23.5 86 20.5 87 12.0 88 6.5 89 7.5 90 8.0 91 5.5 92 3.0 93 3.5 94 2.5 95 1.5 96 1.0 97 1.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07512776847224979 2 0.0776444555054393 3 0.07913582559918124 4 0.08155930200151187 5 0.07960187875347559 6 0.07876298307574577 7 0.07801729802887479 8 0.07811050865973367 9 0.0782969299214514 10-11 0.07741142892829213 12-13 0.0759200588345502 14-15 0.07438208342537883 16-17 0.0731703452242135 18-19 0.07298392396249577 20-21 0.07270429206991916 22-23 0.0754074003648264 24-25 0.07536079504939698 26-27 0.07438208342537883 28-29 0.07084007945274175 30-31 0.07550061099568528 32-33 0.07424226747909053 34-35 0.0713061326070361 36-37 0.06869623494298771 38-39 0.06958173593614699 40-41 0.0720984229693365 42-43 0.07708519172028608 44-45 0.07811050865973367 46-47 0.0806738010083526 48-49 0.08505470065871953 50-51 0.0858003857055905 52-53 0.08598680696730823 54-55 0.08556735912844331 56-57 0.08402938371927196 58-59 0.0827710402026772 60-61 0.0806738010083526 62-63 0.08127967010893526 64-65 0.08062719569292318 66-67 0.0798815106460522 68-69 0.07932224686089898 70-71 0.07741142892829213 72-73 0.07741142892829213 74-75 0.07643271730427398 76-77 0.07671234919685059 78-79 0.07689877045856834 80-81 0.07834353523688084 82-83 0.077504639559151 84-85 0.07661913856599173 86-87 0.07797069271344535 88-89 0.07438208342537883 90-91 0.07163236981504215 92-93 0.07102650071445948 94-95 0.07149255386875385 96-97 0.07065365819102401 98-99 0.07032742098301795 100 0.06953513062071756 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1072839.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.657309648238 #Duplication Level Percentage of deduplicated Percentage of total 1 86.22899323627934 66.96311628405564 2 9.415862111113919 14.624210391357709 3 2.1374199271213277 4.979588433863256 4 0.7848938267792163 2.438109717887363 5 0.4248602119494213 1.6496750518286116 6 0.2441254264553539 1.1374874301150941 7 0.16699303335858626 0.907776079043839 8 0.10554632985092073 0.6557155215574413 9 0.08886290679393723 0.6210768842225366 >10 0.3879093534276569 5.057252757045605 >50 0.012734939170795222 0.637646513616585 >100 0.0015588726215605276 0.16622153253767608 >500 1.1991253902007651E-4 0.06583644217585671 >1k 1.1991253902007651E-4 0.09628696069283713 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 1.8642126171774143E-4 0.0 0.0 9.321063085887071E-5 0.0 10-11 1.8642126171774143E-4 0.0 0.0 9.321063085887071E-5 0.0 12-13 1.8642126171774143E-4 0.0 0.0 9.321063085887071E-5 0.0 14-15 1.8642126171774143E-4 0.0 0.0 9.321063085887071E-5 0.0 16-17 1.8642126171774143E-4 0.0 0.0 9.321063085887071E-5 0.0 18-19 1.8642126171774143E-4 0.0 0.0 9.321063085887071E-5 0.0 20-21 1.8642126171774143E-4 0.0 0.0 1.3981594628830607E-4 0.0 22-23 1.8642126171774143E-4 0.0 0.0 1.8642126171774143E-4 0.0 24-25 1.8642126171774143E-4 0.0 0.0 2.7963189257661214E-4 0.0 26-27 1.8642126171774143E-4 0.0 0.0 4.6605315429435357E-4 0.0 28-29 1.8642126171774143E-4 0.0 0.0 0.0012583435165947547 0.0 30-31 1.8642126171774143E-4 0.0 0.0 0.004846952804661278 0.0 32-33 1.8642126171774143E-4 0.0 0.0 0.012443619219659242 0.0 34-35 1.8642126171774143E-4 0.0 0.0 0.023442473661005984 0.0 36-37 1.8642126171774143E-4 0.0 0.0 0.04101267757790311 0.0 38-39 1.8642126171774143E-4 0.0 0.0 0.0752675844185381 0.0 40-41 1.8642126171774143E-4 0.0 0.0 0.12294482210285046 0.0 42-43 1.8642126171774143E-4 0.0 0.0 0.17313874682035235 0.0 44-45 1.8642126171774143E-4 0.0 0.0 0.22995062632883406 0.0 46-47 1.8642126171774143E-4 0.0 0.0 0.29226193305798914 0.0 48-49 1.8642126171774143E-4 0.0 0.0 0.37424068289836593 0.0 50-51 1.8642126171774143E-4 0.0 0.0 0.46451517888518223 0.0 52-53 1.8642126171774143E-4 0.0 0.0 0.5583316788446355 0.0 54-55 1.8642126171774143E-4 0.0 0.0 0.6551309189915728 0.0 56-57 1.8642126171774143E-4 0.0 0.0 0.7664244122370645 0.0 58-59 1.8642126171774143E-4 0.0 0.0 0.8925383957891166 0.0 60-61 1.8642126171774143E-4 0.0 0.0 1.0236857534075476 0.0 62-63 1.8642126171774143E-4 0.0 0.0 1.1568371395894443 0.0 64-65 1.8642126171774143E-4 0.0 0.0 1.3085374413122566 0.0 66-67 1.8642126171774143E-4 0.0 0.0 1.4723085197312924 0.0 68-69 1.8642126171774143E-4 0.0 0.0 1.6539760392752314 0.0 70-71 1.8642126171774143E-4 0.0 0.0 1.8544721062526623 0.0 72-73 1.8642126171774143E-4 0.0 0.0 2.087778315292416 0.0 74-75 1.8642126171774143E-4 0.0 0.0 2.3146995961183365 0.0 76-77 1.8642126171774143E-4 0.0 0.0 2.5467940669569247 0.0 78-79 1.8642126171774143E-4 0.0 0.0 2.810626757602958 0.0 80-81 1.8642126171774143E-4 0.0 0.0 3.0979485272254275 0.0 82-83 1.8642126171774143E-4 0.0 0.0 3.4030735273419404 0.0 84-85 1.8642126171774143E-4 0.0 0.0 3.699995991942873 0.0 86-87 1.8642126171774143E-4 0.0 0.0 4.005120992059386 0.0 88 1.8642126171774143E-4 0.0 0.0 4.258420881418368 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1075 0.0 32.792973 1 GTATCAA 1520 0.0 29.37704 1 ACCGCGT 65 0.006153299 28.923738 8 GTACATG 3475 0.0 26.240671 1 TCAACGC 1600 0.0 25.851797 4 ATCAACG 1605 0.0 25.771263 3 TACATGG 3540 0.0 24.69663 2 CAACGCA 1715 0.0 24.665287 5 ATACCGT 135 1.4570645E-5 24.370928 6 AACGCAG 1750 0.0 23.903404 6 ACATGGG 3610 0.0 22.91572 3 TCTGTCG 250 4.3655746E-10 22.560513 8 TATCAAC 1895 0.0 22.32345 2 GACCGCG 150 3.2905267E-5 21.933834 7 GAGTACA 2605 0.0 21.652176 1 GTCTCGC 275 1.4351826E-9 20.510513 1 CTATCGC 115 0.0039590118 20.434296 9 CATACCG 165 6.857063E-5 19.93985 5 CATGGGG 2460 0.0 19.68023 4 TATAACG 120 0.005061879 19.584694 2 >>END_MODULE