FastQCFastQC Report
Wed 25 May 2016
SRR1032996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072839
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32600.3038666565999185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25900.24141553392447515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14560.13571467853051575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20700.048.5799941
TAACGCA1250.045.172374
GTATCAA35800.037.277721
CGTATAC550.00270151834.2214933
TATAACG1256.195478E-933.8792762
TCAACGC42750.030.489154
ATCAACG42650.030.4503083
CAACGCA43700.029.820775
GTCGTAC953.7569887E-529.6784821
TATCAAC44350.029.2831022
GTACATG34450.028.9174981
AACGCAG46100.028.5744346
ATAACGC1503.6559868E-828.232733
TACATGG33350.027.2309682
GTCTCGC3250.026.025751
ACGCAGA52300.025.094737
ACATGGG34900.024.808133
CGCAGAG53950.024.3261018
GCAGAGT57850.022.5753259
ACTGTCG1050.002324115322.3996098
Produced by FastQC (version 0.11.5)