##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032996_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1072839 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.80620670948763 34.0 31.0 34.0 31.0 34.0 2 32.85577612297838 34.0 31.0 34.0 31.0 34.0 3 32.92792208336945 34.0 31.0 34.0 31.0 34.0 4 36.34369835548484 37.0 37.0 37.0 35.0 37.0 5 36.327944826763385 37.0 37.0 37.0 35.0 37.0 6 36.38835184030409 37.0 37.0 37.0 35.0 37.0 7 36.35286375681719 37.0 37.0 37.0 35.0 37.0 8 36.295877573429 37.0 37.0 37.0 35.0 37.0 9 38.13066545865689 39.0 39.0 39.0 37.0 39.0 10-11 38.13864428865841 39.0 39.0 39.0 37.0 39.0 12-13 38.12976224764387 39.0 39.0 39.0 37.0 39.0 14-15 39.66514174074581 41.0 40.0 41.0 37.0 41.0 16-17 39.68345529944381 41.0 40.0 41.0 37.0 41.0 18-19 39.714904566295594 41.0 40.0 41.0 37.0 41.0 20-21 39.721962009211076 41.0 40.0 41.0 37.0 41.0 22-23 39.676564703557574 41.0 40.0 41.0 37.0 41.0 24-25 39.652219950989846 41.0 40.0 41.0 37.0 41.0 26-27 39.56466860358357 41.0 40.0 41.0 36.5 41.0 28-29 39.4983012362526 41.0 40.0 41.0 36.0 41.0 30-31 39.37594550533677 41.0 39.0 41.0 36.0 41.0 32-33 39.21997988514586 41.0 39.0 41.0 35.0 41.0 34-35 39.11533230988061 41.0 39.0 41.0 35.0 41.0 36-37 39.00953125305847 41.0 39.0 41.0 35.0 41.0 38-39 38.892639995376754 41.0 39.0 41.0 35.0 41.0 40-41 38.778393589345654 41.0 39.0 41.0 35.0 41.0 42-43 38.632394049806166 41.0 39.0 41.0 35.0 41.0 44-45 38.44727074612314 41.0 38.0 41.0 35.0 41.0 46-47 38.195706438710744 41.0 38.0 41.0 34.0 41.0 48-49 37.962337312495166 40.5 38.0 41.0 34.0 41.0 50-51 37.78610537088976 40.0 38.0 41.0 33.0 41.0 52-53 37.625553321607434 40.0 37.5 41.0 33.0 41.0 54-55 37.46545054756585 40.0 37.0 41.0 33.0 41.0 56-57 37.257037169603265 40.0 37.0 41.0 33.0 41.0 58-59 37.07839153871177 40.0 36.0 41.0 33.0 41.0 60-61 36.868668551385625 39.5 36.0 41.0 32.0 41.0 62-63 36.61682601024012 39.0 35.0 41.0 32.0 41.0 64-65 36.314749929859005 39.0 35.0 41.0 31.5 41.0 66-67 36.02803868986865 38.0 35.0 41.0 31.0 41.0 68-69 35.726408622356196 37.0 35.0 40.0 31.0 41.0 70-71 35.39742356495243 37.0 35.0 40.0 31.0 41.0 72-73 35.0542635008608 36.0 35.0 39.0 30.5 41.0 74-75 34.71680746132458 36.0 35.0 39.0 30.0 41.0 76-77 34.386716925838826 35.5 35.0 38.5 30.0 40.0 78-79 34.05672565967494 35.0 34.0 37.0 30.0 39.0 80-81 33.744281760823384 35.0 34.0 37.0 29.5 39.0 82-83 33.44836597103573 35.0 34.0 36.5 29.0 39.0 84-85 33.18601486336719 35.0 34.0 36.0 29.0 37.0 86-87 33.045816753492375 35.0 34.0 36.0 29.0 37.0 88-89 32.86645293469011 35.0 34.0 35.5 29.0 37.0 90-91 32.70201027367573 35.0 34.0 35.0 29.0 36.0 92-93 32.53703071942761 35.0 34.0 35.0 29.0 36.0 94-95 32.43767704194199 35.0 34.0 35.0 29.0 36.0 96-97 32.374771983494256 35.0 34.0 35.0 29.0 36.0 98-99 32.25602816452422 35.0 34.0 35.0 29.0 36.0 100 32.17261863150016 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10755377693738666 1101 2 0.12056590339656026 1101 3 0.0796347440931271 1101 4 0.06409981955827959 1101 5 0.08104533564195293 1101 6 0.12615312043747196 1101 7 0.05337152253355981 1101 8 -0.06978601173549492 1101 9 0.11409445258359341 1101 10-11 0.13255230710397825 1101 12-13 0.1510946113069167 1101 14-15 0.20202213200580132 1101 16-17 0.15842570260638666 1101 18-19 0.15614105169463244 1101 20-21 0.19768509807557422 1101 22-23 0.23255281764308222 1101 24-25 0.1953485356578284 1101 26-27 0.21616752975142361 1101 28-29 0.18430504914077517 1101 30-31 0.21183292293359557 1101 32-33 0.17785605977437768 1101 34-35 0.25331998127445843 1101 36-37 0.21831179129899425 1101 38-39 0.19471334799232665 1101 40-41 0.22310249806945137 1101 42-43 0.1517350564601614 1101 44-45 0.21120673569568282 1101 46-47 0.0587589533918802 1101 48-49 0.14688266353361001 1101 50-51 0.011652657340647465 1101 52-53 0.14825091162451542 1101 54-55 0.050692195755388525 1101 56-57 0.11078773298201838 1101 58-59 0.055091956271887454 1101 60-61 -0.009594778629775647 1101 62-63 0.027182912197829978 1101 64-65 0.09628494385437847 1101 66-67 0.1646468697922998 1101 68-69 0.09407086416765509 1101 70-71 0.1345496466837588 1101 72-73 0.1799322842617599 1101 74-75 0.1742904532330769 1101 76-77 0.06647368591072222 1101 78-79 0.14883958577643597 1101 80-81 0.03540185006841057 1101 82-83 0.05740429341987863 1101 84-85 0.17613595134394444 1101 86-87 0.08333407357989842 1101 88-89 -0.0032456049321396563 1101 90-91 0.056106130211759364 1101 92-93 0.07637558536232092 1101 94-95 0.17023066565074885 1101 96-97 0.19015959046420505 1101 98-99 0.15392205152830485 1101 100 0.12611887900599328 1102 1 0.17665142064618777 1102 2 0.19454932502935662 1102 3 0.18486379329502967 1102 4 0.1192212833252384 1102 5 0.0903569590286466 1102 6 0.09954321501433583 1102 7 0.08864971313184355 1102 8 0.06567709723777426 1102 9 0.023482085363681904 1102 10-11 0.06503961051002705 1102 12-13 0.055032304402921284 1102 14-15 0.11504573651493644 1102 16-17 0.1482965528149407 1102 18-19 0.14829383813849262 1102 20-21 0.20642097866897302 1102 22-23 0.23555679584616485 1102 24-25 0.219467100857635 1102 26-27 0.14948181346688472 1102 28-29 0.14488982986690502 1102 30-31 0.13104528977534358 1102 32-33 0.14628852675189563 1102 34-35 0.13788922356290811 1102 36-37 0.04454335145797472 1102 38-39 0.13777437105607504 1102 40-41 0.09926509199399192 1102 42-43 0.08258919783763474 1102 44-45 0.06333490417182475 1102 46-47 0.10573091500145892 1102 48-49 0.06999287526326015 1102 50-51 0.01409885516926579 1102 52-53 0.05714386884370981 1102 54-55 0.0601406644119038 1102 56-57 0.03131277448466108 1102 58-59 0.06897454255767599 1102 60-61 -0.058198247834624794 1102 62-63 -0.004796574719854618 1102 64-65 -0.036573850734960445 1102 66-67 -0.005589827618464938 1102 68-69 -0.08247300573149374 1102 70-71 -0.0062998244648113655 1102 72-73 0.056105041133804434 1102 74-75 -0.09609618310546608 1102 76-77 -0.132582058092666 1102 78-79 -0.04556838235256322 1102 80-81 -0.04815459541470801 1102 82-83 -0.033094154248978214 1102 84-85 -0.03767497434458278 1102 86-87 -0.04112161785978685 1102 88-89 -0.16094902459852278 1102 90-91 -0.20259288015350307 1102 92-93 -0.14985611660522835 1102 94-95 -0.18089704336562562 1102 96-97 -0.1838988006324911 1102 98-99 -0.1449177652928384 1102 100 -0.013999805313005709 1103 1 0.13622643520278643 1103 2 0.15759640772563444 1103 3 0.1388474603207328 1103 4 0.05871289832102633 1103 5 -0.0013314584909096538 1103 6 0.0824128215087967 1103 7 0.08993257641814978 1103 8 -0.012004712325612843 1103 9 0.08163317546129889 1103 10-11 0.1030198942191447 1103 12-13 0.13774941960108578 1103 14-15 0.14778161056303674 1103 16-17 0.17927622198681803 1103 18-19 0.15111775103288494 1103 20-21 0.12901281971226553 1103 22-23 0.08645016086111212 1103 24-25 0.13186259927153543 1103 26-27 0.08822441918544399 1103 28-29 0.09346503133964745 1103 30-31 0.06199524062581929 1103 32-33 0.12503790986139762 1103 34-35 0.13068622860157575 1103 36-37 0.13840752662662936 1103 38-39 0.14510175265680658 1103 40-41 0.08126130257183206 1103 42-43 0.1760035640238371 1103 44-45 0.09631484497722909 1103 46-47 0.10588286840958716 1103 48-49 -0.019886893283462825 1103 50-51 0.016631636091453572 1103 52-53 0.05331948395244268 1103 54-55 0.05021528294834354 1103 56-57 0.11925480089765017 1103 58-59 0.06427608938957974 1103 60-61 -0.03346077087977761 1103 62-63 0.1041291415791008 1103 64-65 0.1718766413654791 1103 66-67 0.15252912366096893 1103 68-69 0.20414003348458465 1103 70-71 0.21264621055977528 1103 72-73 0.135903440647553 1103 74-75 0.03471284104436023 1103 76-77 0.10582611565597233 1103 78-79 0.19604762197205616 1103 80-81 0.19796286053561118 1103 82-83 0.21237178465499085 1103 84-85 0.11249466213713788 1103 86-87 0.2335254266464304 1103 88-89 0.23555994670379476 1103 90-91 0.23157431431593523 1103 92-93 0.1099035280658498 1103 94-95 -0.0099521486639631 1103 96-97 0.017526199602841075 1103 98-99 0.025828110785802494 1103 100 -0.0013486755461400435 1104 1 0.12001358546176277 1104 2 0.21173063521882085 1104 3 0.10402253454532939 1104 4 0.05958763851891291 1104 5 0.08140484861689856 1104 6 0.08571715795311974 1104 7 0.07567666379464555 1104 8 0.1511687681180618 1104 9 0.14243553251185403 1104 10-11 0.13992345079729773 1104 12-13 0.18142654508074685 1104 14-15 0.12077716157878626 1104 16-17 0.16062179911644847 1104 18-19 0.2089181879324613 1104 20-21 0.16298375096195628 1104 22-23 0.1307727547801889 1104 24-25 0.12453959086676036 1104 26-27 0.10671116569514538 1104 28-29 0.0899168797790324 1104 30-31 0.1577681709028198 1104 32-33 0.24520903385335657 1104 34-35 0.2106389797985102 1104 36-37 0.1304264312808101 1104 38-39 0.10374258176507567 1104 40-41 0.17429601799467775 1104 42-43 0.21464847382721075 1104 44-45 0.001721637666371123 1104 46-47 -0.06876147163635693 1104 48-49 -0.043660791976996904 1104 50-51 -0.13449951633705126 1104 52-53 -0.03641538635715591 1104 54-55 -0.16024335895013309 1104 56-57 -0.14600128838488047 1104 58-59 -0.16349599223972433 1104 60-61 -0.03767107296670247 1104 62-63 0.014324120955329533 1104 64-65 -0.1393758112221093 1104 66-67 0.0021129483673121285 1104 68-69 -0.12688774988953355 1104 70-71 -0.12376967301123187 1104 72-73 0.05825901975764225 1104 74-75 -0.017635119912704056 1104 76-77 -0.04446922144409626 1104 78-79 -0.017943986510481125 1104 80-81 -0.06258866636724747 1104 82-83 -0.07491813333329844 1104 84-85 -0.06183592635127866 1104 86-87 -0.058974649278454194 1104 88-89 -0.037214844695263594 1104 90-91 -0.04270400363351712 1104 92-93 -0.08882739286662655 1104 94-95 -0.01086260563764796 1104 96-97 -0.002245709217149283 1104 98-99 -0.09034829501153041 1104 100 -0.1371686664180416 1105 1 0.05718405234019741 1105 2 0.12303598581433306 1105 3 0.06847910964431492 1105 4 0.02374784594316992 1105 5 0.06269477650351973 1105 6 0.06102592257178685 1105 7 0.09038949759115411 1105 8 0.008867994664662149 1105 9 0.038779696510793826 1105 10-11 0.08321105410171725 1105 12-13 0.1349537343313827 1105 14-15 0.1318541103825126 1105 16-17 0.1315011915461568 1105 18-19 0.18836678676735374 1105 20-21 0.13598117885479866 1105 22-23 0.2346999162153054 1105 24-25 0.151541335131256 1105 26-27 0.08924844552003464 1105 28-29 0.19516921036311174 1105 30-31 0.23888785294500536 1105 32-33 0.16790570650533 1105 34-35 0.19061822578285614 1105 36-37 0.1753733210345203 1105 38-39 0.22077262997035518 1105 40-41 0.16875236957614703 1105 42-43 0.10786877352281721 1105 44-45 0.16405372012943076 1105 46-47 0.21545119617769615 1105 48-49 0.23854311560595676 1105 50-51 0.2912717639795801 1105 52-53 0.2144271505170181 1105 54-55 0.25319037514183407 1105 56-57 0.25804429594373346 1105 58-59 0.20929004602813706 1105 60-61 0.22418425490452165 1105 62-63 0.17152999466462404 1105 64-65 0.15010814477308543 1105 66-67 0.3369167372376296 1105 68-69 0.3064878862040743 1105 70-71 0.1853666470128701 1105 72-73 0.28061921575767457 1105 74-75 0.25043192540919534 1105 76-77 0.29701587862967216 1105 78-79 0.2703222129217764 1105 80-81 0.24309534277544742 1105 82-83 0.24835339326652672 1105 84-85 0.3939574821998377 1105 86-87 0.2973406312898419 1105 88-89 0.29773857673708903 1105 90-91 0.24552784032806585 1105 92-93 0.2755231355867451 1105 94-95 0.2982561227297893 1105 96-97 0.2563870341127128 1105 98-99 0.23785758780402944 1105 100 0.1501150907293436 1106 1 0.0016724109784220786 1106 2 0.02853647667448911 1106 3 0.04708363825587014 1106 4 0.07557982177237221 1106 5 0.047956273623043444 1106 6 0.0179365749118503 1106 7 -0.0027108418443972937 1106 8 -0.09883910507002724 1106 9 0.005269463048655609 1106 10-11 -0.0065677453424228815 1106 12-13 -0.03149329523054689 1106 14-15 0.06417691462652897 1106 16-17 0.039139324833143974 1106 18-19 0.05288243834155537 1106 20-21 0.04630535290420568 1106 22-23 0.03573826203640351 1106 24-25 0.07412828325087162 1106 26-27 0.07808425378855333 1106 28-29 0.043331123582838416 1106 30-31 -0.014869985143363351 1106 32-33 0.10683805986432304 1106 34-35 -0.03688791889748444 1106 36-37 -0.03446880214796977 1106 38-39 0.0858559254078557 1106 40-41 0.009021235550292772 1106 42-43 0.10314429408457926 1106 44-45 0.0833915469958697 1106 46-47 0.07823197255418535 1106 48-49 0.061382874564657186 1106 50-51 0.11576663832017431 1106 52-53 0.01169674583714908 1106 54-55 0.1064374726384898 1106 56-57 0.07315350340100224 1106 58-59 0.0041110943657542975 1106 60-61 -0.03020749515281551 1106 62-63 -0.14374250216680906 1106 64-65 -0.098618872693919 1106 66-67 -0.09091193867800484 1106 68-69 0.04661850183081384 1106 70-71 -0.03135664515487946 1106 72-73 -0.06152182340558454 1106 74-75 -0.03575224595774529 1106 76-77 -0.12509925645012743 1106 78-79 -0.18536763805831669 1106 80-81 -0.07713218301157099 1106 82-83 -0.029153029184314505 1106 84-85 0.009800678370098126 1106 86-87 -0.09337393729685317 1106 88-89 -0.15756378439155583 1106 90-91 -0.13855833043095345 1106 92-93 -0.16326378507326922 1106 94-95 -0.17190795233809553 1106 96-97 -0.1975576244795576 1106 98-99 -0.23209696894170406 1106 100 -0.27965619303862255 1107 1 0.008259731510740664 1107 2 -0.00942181913260498 1107 3 0.0037446274571522054 1107 4 0.04867624681426008 1107 5 0.007917649945206051 1107 6 0.021334213132377045 1107 7 0.028816580851547258 1107 8 0.09780146524424538 1107 9 0.06736332919501109 1107 10-11 0.014611852837560946 1107 12-13 -0.034333565488658735 1107 14-15 0.018397208739990845 1107 16-17 0.0989950703166329 1107 18-19 0.07871625120353798 1107 20-21 0.07283856646108688 1107 22-23 0.02560069977936763 1107 24-25 0.07974847427048104 1107 26-27 0.14361535050034746 1107 28-29 0.08547140887066718 1107 30-31 0.08948025258368375 1107 32-33 0.04940302110034622 1107 34-35 0.019050490789496166 1107 36-37 0.012971091483159114 1107 38-39 -0.08152974593031814 1107 40-41 0.001225579714628111 1107 42-43 0.0529014161900605 1107 44-45 0.2502644398086318 1107 46-47 0.13215520876327247 1107 48-49 0.06335749471838881 1107 50-51 0.10396389122646355 1107 52-53 0.14138320137282534 1107 54-55 0.06500312270443231 1107 56-57 0.1788125948604673 1107 58-59 0.11962541237992497 1107 60-61 0.1277594264039834 1107 62-63 0.15153067155940647 1107 64-65 0.1843374300422198 1107 66-67 0.2703482203140908 1107 68-69 0.2155027242044767 1107 70-71 0.2604704569968348 1107 72-73 0.2628052124385576 1107 74-75 0.23502556679967768 1107 76-77 0.2570814207173484 1107 78-79 0.23589452745844852 1107 80-81 0.2019150483495622 1107 82-83 0.1507130718115235 1107 84-85 0.18634159898053326 1107 86-87 0.12143260028339853 1107 88-89 0.2099219825448415 1107 90-91 0.16069483493858883 1107 92-93 0.15452936068459877 1107 94-95 0.09146301678641322 1107 96-97 0.16107733140957237 1107 98-99 0.1766353725841654 1107 100 0.1349433111729681 1108 1 0.004503606863607956 1108 2 0.044852641699336004 1108 3 0.0130954918767614 1108 4 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44-45 -0.3723826104355936 2214 46-47 -0.3756504571617043 2214 48-49 -0.315756121704716 2214 50-51 -0.3338890328010251 2214 52-53 -0.37432252213761075 2214 54-55 -0.36268041089982006 2214 56-57 -0.3695395332290161 2214 58-59 -0.3162356181869157 2214 60-61 -0.34877129988412037 2214 62-63 -0.5417670616969801 2214 64-65 -0.4874288444074324 2214 66-67 -0.49807937521894985 2214 68-69 -0.5668187993555804 2214 70-71 -0.4332748172748495 2214 72-73 -0.46664174092311583 2214 74-75 -0.36732612931685793 2214 76-77 -0.2883740632906395 2214 78-79 -0.3642725588905833 2214 80-81 -0.46553395475324066 2214 82-83 -0.466077106998398 2214 84-85 -0.407586133059894 2214 86-87 -0.541954447804244 2214 88-89 -0.5329797154234797 2214 90-91 -0.4120930981364097 2214 92-93 -0.5251653216235539 2214 94-95 -0.626515513699303 2214 96-97 -0.5671900539447137 2214 98-99 -0.6189480154012799 2214 100 -0.5984363002939155 2215 1 -0.14930884804159916 2215 2 -0.23405457933789364 2215 3 -0.152357810592747 2215 4 -0.07540757304313672 2215 5 -0.10657308987213554 2215 6 -0.07278535955592247 2215 7 -0.16332045573170717 2215 8 -0.0819811684347087 2215 9 -0.15296915028739733 2215 10-11 -0.1553955016144215 2215 12-13 -0.17603245339294205 2215 14-15 -0.20312740113055128 2215 16-17 -0.18001821107014138 2215 18-19 -0.22653862028984406 2215 20-21 -0.16651164570361487 2215 22-23 -0.23002080206747166 2215 24-25 -0.25534068302582824 2215 26-27 -0.23115128099285442 2215 28-29 -0.12779266027827418 2215 30-31 -0.19910806305052375 2215 32-33 -0.22713070506139843 2215 34-35 -0.10217132967639486 2215 36-37 -0.15301220951021577 2215 38-39 -0.1470858365278005 2215 40-41 -0.19675655241793066 2215 42-43 -0.2569351870725569 2215 44-45 -0.2944467024552182 2215 46-47 -0.2134517962968303 2215 48-49 -0.33498612313377407 2215 50-51 -0.38219498583134737 2215 52-53 -0.4436175473692643 2215 54-55 -0.3009217035889833 2215 56-57 -0.3188723199016579 2215 58-59 -0.33957273390560516 2215 60-61 -0.26885796891696145 2215 62-63 -0.2551401298099236 2215 64-65 -0.30379793619714235 2215 66-67 -0.35498914894493794 2215 68-69 -0.34885854496561564 2215 70-71 -0.33631197817840786 2215 72-73 -0.3684708055578483 2215 74-75 -0.32341351646139316 2215 76-77 -0.31115304541686584 2215 78-79 -0.35474078258568653 2215 80-81 -0.3941732569953871 2215 82-83 -0.44291910617950236 2215 84-85 -0.4941136362147205 2215 86-87 -0.5564253071454885 2215 88-89 -0.5969182276601472 2215 90-91 -0.5787767905852519 2215 92-93 -0.5295885372275038 2215 94-95 -0.5713614790157209 2215 96-97 -0.46539882234185015 2215 98-99 -0.45055453975443527 2215 100 -0.4974094391166375 2216 1 -0.18274104902530297 2216 2 -0.2405521519853906 2216 3 -0.15708468514336715 2216 4 -0.045268626786118205 2216 5 -0.18669970684309334 2216 6 -0.17560253011571092 2216 7 -0.09753859360242245 2216 8 -0.06401671152355703 2216 9 -0.11911534217153985 2216 10-11 -0.1426189386148735 2216 12-13 -0.16695247223821497 2216 14-15 -0.28570768863757223 2216 16-17 -0.2577126786563042 2216 18-19 -0.3243070065483522 2216 20-21 -0.2772985134452455 2216 22-23 -0.27826253697304537 2216 24-25 -0.25322097927095655 2216 26-27 -0.28682805508118037 2216 28-29 -0.338622155456882 2216 30-31 -0.32999814934515115 2216 32-33 -0.45406217004765637 2216 34-35 -0.45511821054852675 2216 36-37 -0.4302456058771824 2216 38-39 -0.428082193230253 2216 40-41 -0.36777075093266376 2216 42-43 -0.46585228163416303 2216 44-45 -0.37438105516510234 2216 46-47 -0.43959021982323776 2216 48-49 -0.5740349038049857 2216 50-51 -0.5341965436515466 2216 52-53 -0.4977674865266408 2216 54-55 -0.4661280434608557 2216 56-57 -0.468033733981315 2216 58-59 -0.3791885061610003 2216 60-61 -0.5671509786136681 2216 62-63 -0.3943709586776194 2216 64-65 -0.40588948095167154 2216 66-67 -0.5694002304190846 2216 68-69 -0.49385315300970234 2216 70-71 -0.5137685203620634 2216 72-73 -0.5347359601432444 2216 74-75 -0.5885732876734053 2216 76-77 -0.5185075228923921 2216 78-79 -0.5636271511662514 2216 80-81 -0.5815528649526058 2216 82-83 -0.6129775581449479 2216 84-85 -0.5346723201159307 2216 86-87 -0.5390661650252468 2216 88-89 -0.6369401792344433 2216 90-91 -0.694907386116995 2216 92-93 -0.7098240304806183 2216 94-95 -0.6511017524276852 2216 96-97 -0.7037038268392521 2216 98-99 -0.6656157933589029 2216 100 -0.6457010616066015 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 1.0 5 8.0 6 27.0 7 73.0 8 149.0 9 315.0 10 598.0 11 880.0 12 1434.0 13 2334.0 14 2581.0 15 3149.0 16 3886.0 17 5684.0 18 6983.0 19 4000.0 20 2635.0 21 2381.0 22 2359.0 23 2492.0 24 2727.0 25 3373.0 26 4023.0 27 5140.0 28 6549.0 29 8489.0 30 10675.0 31 13546.0 32 17134.0 33 22396.0 34 31797.0 35 49342.0 36 92459.0 37 213884.0 38 367232.0 39 181262.0 40 833.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.68026799995526 19.051560536745598 12.858722115961807 23.40944934733734 2 16.567272415438765 19.618355902881348 39.86355738829565 23.95081429338424 3 18.700662447953516 24.844827602277693 29.763552592700304 26.69095735706849 4 12.528595692794164 16.555204835585254 38.16683664052115 32.74936283109944 5 12.298372626494524 37.05627996155972 34.943555986812484 15.701791425133269 6 30.839793847682557 37.66893891586401 17.339594835565656 14.151672400887772 7 27.46213286354781 32.28386603655752 21.88156557888948 18.372435521005194 8 25.679647763941354 35.24292769621227 20.119907367218413 18.957517172627966 9 25.106354420279857 16.236526265160865 21.266969169335624 37.39015014522365 10-11 24.214537316410013 26.23958487713441 28.17654839169717 21.36932941475841 12-13 25.418364460261262 23.53769929024732 28.68712299195079 22.35681325754063 14-15 22.396376335070627 25.447773262747862 26.43729962580505 25.71855077637646 16-17 21.22527238476603 29.043873311838965 26.701676579617256 23.029177723777753 18-19 20.94911857573957 27.09827909632167 29.97369126265627 21.978911065282485 20-21 22.340490977676986 26.549183987532142 29.622618118841693 21.48770691594918 22-23 21.856898178894753 26.54264974891351 29.805259417199714 21.795192654992018 24-25 21.563670817819524 26.486331302731227 29.875064606889506 22.074933272559747 26-27 21.384543895733216 26.303696028986195 30.110138410578436 22.201621664702156 28-29 21.57827914429829 26.429750633181605 30.067853204406962 21.924117018113144 30-31 21.877747169512304 27.068598250201596 29.41744857578342 21.63620600450268 32-33 21.299366715113162 26.668630629807556 30.10904656736844 21.922956087710844 34-35 21.26777726188678 26.14294717959906 30.202278995094062 22.386996563420098 36-37 22.087004197337816 26.865852561224578 29.295720934612817 21.75142230682479 38-39 21.8651901327976 26.962766039285857 29.41707155255653 21.754972275360014 40-41 21.4925886740933 26.64813349479139 29.704334981019976 22.15494285009533 42-43 21.44951111202311 26.813661068138384 29.43517885474389 22.30164896509462 44-45 21.83587045316386 27.011986800663045 29.149624365681166 22.002518380491928 46-47 21.83208072905488 27.60098130518442 28.622832049001808 21.944105916758893 48-49 21.7905776603294 27.160575042935065 28.735910510307917 22.31293678642762 50-51 21.819673982115578 27.042604037291714 28.636581225931007 22.501140754661698 52-53 21.94854315075811 27.17512656134312 28.425367413959044 22.450962873939723 54-55 22.057787469987897 27.866301622449846 28.089835216875642 21.98607569068661 56-57 21.55173097458084 27.459124032768884 28.317252272908572 22.671892719741706 58-59 21.494562885066777 26.79966825015953 28.893909087855867 22.811859776917824 60-61 22.08004664723032 27.379965014577262 28.180338192419825 22.359650145772594 62-63 21.74537298993223 27.242508644813274 28.392631489090952 22.619486876163545 64-65 21.728842817981075 26.942299823179432 28.682868538522555 22.645988820316933 66-67 21.61321503040065 27.117862046402113 28.326058243594797 22.942864679602437 68-69 22.229534081783978 27.22152495459475 28.243346971608496 22.305593992012778 70-71 22.391710219333934 26.9435115613806 28.24850699872022 22.41627122056525 72-73 21.896621953527042 27.17019981562937 28.600470340843316 22.33270789000027 74-75 21.93982508093013 27.18665149197596 28.342183682733385 22.531339744360523 76-77 22.03999017559493 27.239934062769 27.979700579350947 22.740375182285124 78-79 22.205195746985336 27.477282238993922 27.98616567816793 22.331356335852817 80-81 21.996175558903897 27.13204668185693 28.233985327680404 22.637792431558772 82-83 21.721209669488932 27.299216051623798 28.368528907193397 22.611045371693876 84-85 21.91652242321541 27.195506501907555 28.127333178603685 22.760637896273344 86-87 22.136281419775614 27.360960225347597 28.00593569446833 22.496822660408462 88-89 21.634355020772105 27.292038928705587 28.145107393228958 22.928498657293353 90-91 21.47022992266314 27.19354907865952 28.168578882758737 23.167642115918603 92-93 21.855938301616657 27.471262975004475 27.81306858855813 22.859730134820737 94-95 21.59797992132021 27.189269124310993 28.08083670331586 23.13191425105294 96-97 21.56261534847824 27.10866339310016 28.167399304273903 23.1613219541477 98-99 21.404516427907634 27.230553699110494 28.11875780056467 23.246172072417203 100 21.723296785444973 27.178728588352957 27.860843984978175 23.237130641223892 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 59.0 1 50.0 2 188.5 3 393.0 4 268.5 5 100.0 6 255.0 7 664.5 8 1145.0 9 1268.0 10 919.5 11 594.5 12 558.0 13 636.0 14 720.5 15 838.5 16 934.5 17 1025.5 18 1184.0 19 1376.5 20 1599.5 21 1866.5 22 2373.0 23 3132.0 24 4102.5 25 5393.0 26 7096.5 27 9271.5 28 11707.5 29 14361.5 30 16996.5 31 19388.0 32 21662.0 33 23925.5 34 26147.5 35 28130.0 36 29819.5 37 31328.5 38 32761.0 39 33120.5 40 33268.0 41 34198.5 42 34841.5 43 35193.5 44 35254.0 45 35486.5 46 35808.5 47 35371.0 48 35341.5 49 35405.5 50 35114.0 51 34228.0 52 31796.0 53 30127.0 54 29299.5 55 27934.0 56 26218.0 57 24416.0 58 22631.0 59 21036.0 60 19122.0 61 16558.0 62 14105.5 63 11813.0 64 9537.0 65 7519.5 66 5920.0 67 4542.5 68 3517.0 69 2722.0 70 1983.0 71 1410.0 72 1067.5 73 801.5 74 614.0 75 450.5 76 288.0 77 207.0 78 143.0 79 88.5 80 58.0 81 34.0 82 15.5 83 7.0 84 4.0 85 3.5 86 2.0 87 1.5 88 2.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.7963189257661214E-4 2 6.52474416012095E-4 3 0.0 4 0.01165132885735884 5 5.592637851532243E-4 6 0.003728425234354829 7 8.388956777298364E-4 8 0.14093447385861252 9 0.0010253169394475777 10-11 0.0 12-13 4.660531542943536E-5 14-15 2.330265771471768E-4 16-17 0.0 18-19 1.3981594628830607E-4 20-21 0.0 22-23 1.3981594628830607E-4 24-25 4.194478388649182E-4 26-27 0.004893558120090713 28-29 0.007643271730427399 30-31 0.013422330843677383 32-33 0.016591492292878988 34-35 0.02432797465416526 36-37 0.03481417062578821 38-39 0.03891543838357852 40-41 0.049168607778054305 42-43 0.06636596917151595 44-45 0.10425609061564689 46-47 0.11231881018493922 48-49 0.1083573583734372 50-51 0.10998854441346745 52-53 0.10490856503165898 54-55 0.11073422946033841 56-57 0.0959603444692074 58-59 0.08827046742335057 60-61 0.08985504814795137 62-63 0.08887633652393323 64-65 0.0 66-67 0.0 68-69 4.660531542943536E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 4.660531542943536E-5 78-79 0.0 80-81 2.330265771471768E-4 82-83 4.660531542943536E-5 84-85 0.0 86-87 6.990797314415304E-4 88-89 5.592637851532243E-4 90-91 0.0 92-93 6.52474416012095E-4 94-95 8.855009931592717E-4 96-97 2.7963189257661214E-4 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1072839.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.28185152976931 #Duplication Level Percentage of deduplicated Percentage of total 1 85.29244718937183 64.20973345920979 2 9.949342687790068 14.980098780820155 3 2.285101146725062 5.160799357747851 4 0.8854073827115106 2.666204285145983 5 0.45904495374591203 1.7278877026694783 6 0.27670198075061414 1.249838245971649 7 0.17118461449331207 0.9020966312726422 8 0.11869389354793702 0.7148396857252829 9 0.09736079910658557 0.6596551100845504 >10 0.4448552118227923 5.6284958560515 >50 0.015769111345140203 0.8011435450493889 >100 0.0034711773628702874 0.4962210062667144 >500 2.479404905794679E-4 0.12113857060814062 >1k 3.7191073586920187E-4 0.6818477633767914 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3260 0.3038666565999185 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2590 0.24141553392447515 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1456 0.13571467853051575 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.321063085887071E-5 0.0 10-11 4.660531542943536E-5 0.0 0.0 9.321063085887071E-5 0.0 12-13 1.8642126171774143E-4 0.0 0.0 9.321063085887071E-5 0.0 14-15 1.8642126171774143E-4 0.0 0.0 9.321063085887071E-5 0.0 16-17 1.8642126171774143E-4 0.0 0.0 9.321063085887071E-5 0.0 18-19 1.8642126171774143E-4 0.0 0.0 9.321063085887071E-5 0.0 20-21 1.8642126171774143E-4 0.0 0.0 1.3981594628830607E-4 0.0 22-23 1.8642126171774143E-4 0.0 0.0 1.8642126171774143E-4 0.0 24-25 1.8642126171774143E-4 0.0 0.0 2.7963189257661214E-4 0.0 26-27 1.8642126171774143E-4 0.0 0.0 5.592637851532243E-4 0.0 28-29 2.3302657714717679E-4 0.0 0.0 0.00130494883202419 0.0 30-31 2.7963189257661214E-4 0.0 0.0 0.004846952804661278 0.0 32-33 2.7963189257661214E-4 0.0 0.0 0.012583435165947546 0.0 34-35 2.7963189257661214E-4 0.0 0.0 0.02362889492272373 0.0 36-37 2.7963189257661214E-4 0.0 0.0 0.041292309470479724 0.0 38-39 2.7963189257661214E-4 0.0 0.0 0.07559382162654416 0.0 40-41 2.7963189257661214E-4 0.0 0.0 0.1233642699417154 0.0 42-43 2.7963189257661214E-4 0.0 0.0 0.17346498402835842 0.0 44-45 2.7963189257661214E-4 0.0 0.0 0.23037007416769897 0.0 46-47 2.7963189257661214E-4 0.0 0.0 0.2925415649505657 0.0 48-49 2.7963189257661214E-4 0.0 0.0 0.37400765632121874 0.0 50-51 2.7963189257661214E-4 0.0 0.0 0.46451517888518223 0.0 52-53 2.7963189257661214E-4 0.0 0.0 0.5585181001063533 0.0 54-55 2.7963189257661214E-4 0.0 0.0 0.6558766040384438 0.0 56-57 2.7963189257661214E-4 0.0 0.0 0.7678691770153769 0.0 58-59 2.7963189257661214E-4 0.0 0.0 0.895381320030312 0.0 60-61 2.7963189257661214E-4 0.0 0.0 1.0271811520647554 0.0 62-63 2.7963189257661214E-4 0.0 0.0 1.1608451967163758 0.0 64-65 2.7963189257661214E-4 0.0 0.0 1.3131513675397706 0.0 66-67 2.7963189257661214E-4 0.0 0.0 1.4787400532605544 0.0 68-69 2.7963189257661214E-4 0.0 0.0 1.6618989428982354 0.0 70-71 2.7963189257661214E-4 0.0 0.0 1.863979590600267 0.0 72-73 2.7963189257661214E-4 0.0 0.0 2.098683959102903 0.0 74-75 2.7963189257661214E-4 0.0 0.0 2.327189820653425 0.0 76-77 2.7963189257661214E-4 0.0 0.0 2.5598435552771663 0.0 78-79 2.7963189257661214E-4 0.0 0.0 2.8262861435872484 0.0 80-81 2.7963189257661214E-4 0.0 0.0 3.116963495920637 0.0 82-83 2.7963189257661214E-4 0.0 0.0 3.425304262801781 0.0 84-85 2.7963189257661214E-4 0.0 0.0 3.7239511240736025 0.0 86-87 2.7963189257661214E-4 0.0 0.0 4.03121996869987 0.0 88 2.7963189257661214E-4 0.0 0.0 4.285265543105722 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2070 0.0 48.579994 1 TAACGCA 125 0.0 45.17237 4 GTATCAA 3580 0.0 37.27772 1 CGTATAC 55 0.002701518 34.221493 3 TATAACG 125 6.195478E-9 33.879276 2 TCAACGC 4275 0.0 30.48915 4 ATCAACG 4265 0.0 30.450308 3 CAACGCA 4370 0.0 29.82077 5 GTCGTAC 95 3.7569887E-5 29.678482 1 TATCAAC 4435 0.0 29.283102 2 GTACATG 3445 0.0 28.917498 1 AACGCAG 4610 0.0 28.574434 6 ATAACGC 150 3.6559868E-8 28.23273 3 TACATGG 3335 0.0 27.230968 2 GTCTCGC 325 0.0 26.02575 1 ACGCAGA 5230 0.0 25.09473 7 ACATGGG 3490 0.0 24.80813 3 CGCAGAG 5395 0.0 24.326101 8 GCAGAGT 5785 0.0 22.575325 9 ACTGTCG 105 0.0023241153 22.399609 8 >>END_MODULE