FastQCFastQC Report
Wed 25 May 2016
SRR1032995_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032995_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532916
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC23150.035.3290024
GTATCAA25250.034.6234661
CAACGCA23900.034.4158975
ATCAACG24100.033.9363673
AACGCAG24650.033.1780856
GGTATCA14450.033.178081
TATCAAC29200.028.6520792
GTACATG49200.027.99121
TACATGG50500.026.432972
ACGCAGA30600.026.4195867
ACATGGG51400.025.2394073
CGCAGAG31750.025.1657568
GAGTACA38300.020.4944671
GCAGAGT36600.020.2899429
CATGGGG37550.019.7779064
AGTACAT38300.019.1445332
CCGGTCG1801.3377021E-418.278079
TACACTG11900.017.3789965
CATACAC8450.017.2440053
CTTACAC6400.016.8919983
Produced by FastQC (version 0.11.5)