##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032995_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1532916 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.59494518942982 33.0 31.0 34.0 30.0 34.0 2 31.68625482413909 34.0 31.0 34.0 30.0 34.0 3 31.749164337771933 34.0 31.0 34.0 30.0 34.0 4 35.04405981802004 37.0 35.0 37.0 33.0 37.0 5 35.11526071878694 37.0 35.0 37.0 33.0 37.0 6 35.143382938138814 37.0 36.0 37.0 33.0 37.0 7 35.10212236025979 37.0 36.0 37.0 33.0 37.0 8 35.09977715673918 37.0 36.0 37.0 33.0 37.0 9 36.712969269027134 39.0 38.0 39.0 33.0 39.0 10-11 36.712376933895925 39.0 38.0 39.0 33.0 39.0 12-13 36.658173050578114 39.0 38.0 39.0 33.0 39.0 14-15 38.13169540927226 41.0 39.0 41.0 33.5 41.0 16-17 38.15702165024045 41.0 39.0 41.0 33.5 41.0 18-19 38.17072200955565 41.0 39.0 41.0 33.5 41.0 20-21 38.219015914766366 41.0 39.0 41.0 34.0 41.0 22-23 38.21738699315553 41.0 39.0 41.0 34.0 41.0 24-25 38.25662104120513 41.0 39.0 41.0 34.0 41.0 26-27 38.23865234624728 41.0 39.0 41.0 33.5 41.0 28-29 38.22300145604847 41.0 39.0 41.0 33.0 41.0 30-31 38.18108787435189 41.0 39.0 41.0 33.0 41.0 32-33 38.125157542879066 41.0 39.0 41.0 33.0 41.0 34-35 38.05661725756663 41.0 39.0 41.0 33.0 41.0 36-37 37.963974216460656 41.0 38.0 41.0 33.0 41.0 38-39 37.86234601243643 41.0 38.0 41.0 33.0 41.0 40-41 37.77294450576548 40.5 38.0 41.0 32.5 41.0 42-43 37.65326541049868 40.0 38.0 41.0 32.0 41.0 44-45 37.51764284540053 40.0 38.0 41.0 32.0 41.0 46-47 37.390041267753745 40.0 38.0 41.0 31.5 41.0 48-49 37.25976178733864 40.0 37.5 41.0 31.0 41.0 50-51 37.1156067912397 40.0 37.0 41.0 31.0 41.0 52-53 36.95698720608305 40.0 37.0 41.0 30.5 41.0 54-55 36.7885856106923 40.0 36.0 41.0 30.0 41.0 56-57 36.60093638529443 40.0 36.0 41.0 30.0 41.0 58-59 36.39167018936458 39.5 35.0 41.0 30.0 41.0 60-61 36.1450281685363 39.0 35.0 41.0 30.0 41.0 62-63 35.8850723066365 39.0 35.0 41.0 30.0 41.0 64-65 35.59867305188282 38.0 35.0 40.5 29.5 41.0 66-67 35.298991921279445 37.5 35.0 40.0 29.0 41.0 68-69 34.97964630808211 37.0 35.0 40.0 29.0 41.0 70-71 34.6428617093174 36.5 34.0 39.5 29.0 41.0 72-73 34.35129909270958 36.0 34.0 39.0 28.5 41.0 74-75 34.07487168246662 36.0 34.0 39.0 28.0 40.5 76-77 33.756156240785536 35.0 34.0 38.0 28.0 40.0 78-79 33.3994083824554 35.0 34.0 37.0 28.0 39.0 80-81 33.08885940260262 35.0 34.0 37.0 27.0 39.0 82-83 32.78522176035739 35.0 34.0 36.0 27.0 39.0 84-85 32.6300524621049 35.0 34.0 36.0 27.0 37.0 86-87 32.41039169791431 35.0 34.0 36.0 27.0 37.0 88-89 32.19067189591602 35.0 34.0 35.0 26.5 37.0 90-91 32.007501715684356 35.0 33.0 35.0 26.0 36.0 92-93 31.831677012960917 35.0 33.0 35.0 26.0 36.0 94-95 31.66749874096167 35.0 33.0 35.0 25.5 36.0 96-97 31.511466381719547 35.0 33.0 35.0 25.0 36.0 98-99 31.34585619825222 35.0 33.0 35.0 25.0 35.0 100 31.226046958867936 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3744572207765948 1101 2 0.4582720728213161 1101 3 0.45509645120208475 1101 4 0.5044329722804761 1101 5 0.3483278908454679 1101 6 0.3682776522234832 1101 7 0.32877081041961276 1101 8 0.30690412297378344 1101 9 0.4072302059247974 1101 10-11 0.3370126925055956 1101 12-13 8.054595868074443E-4 1101 14-15 0.06233353452194734 1101 16-17 0.08975863789257232 1101 18-19 0.16180771695636764 1101 20-21 0.17702651943461234 1101 22-23 0.05920968381145997 1101 24-25 0.034158772304536456 1101 26-27 0.04369444636371611 1101 28-29 0.11215112565292173 1101 30-31 0.15044455991814942 1101 32-33 0.14792758348396973 1101 34-35 0.11254963431814247 1101 36-37 0.1497437760757876 1101 38-39 0.13107268506755787 1101 40-41 0.13673365847546393 1101 42-43 0.12623825508154596 1101 44-45 0.16186433269731992 1101 46-47 0.11171112709748598 1101 48-49 0.1667719999279882 1101 50-51 0.21309357861530032 1101 52-53 0.19813847554966202 1101 54-55 0.1240703195118229 1101 56-57 0.11931140983968902 1101 58-59 0.05058406802459814 1101 60-61 0.14360789462249102 1101 62-63 -0.002829398118997517 1101 64-65 0.07402205711497345 1101 66-67 0.07032073614529111 1101 68-69 0.11327283351641881 1101 70-71 0.12495808886723836 1101 72-73 0.1314720512260692 1101 74-75 0.20061862988911372 1101 76-77 0.11684147677920009 1101 78-79 0.1071971829207925 1101 80-81 0.15913419527759487 1101 82-83 0.03500028845215297 1101 84-85 0.06434742060142185 1101 86-87 0.08138179775160381 1101 88-89 0.13175752604612256 1101 90-91 0.16557280734014057 1101 92-93 0.18651432318566563 1101 94-95 0.2084374644016478 1101 96-97 0.18321526807656596 1101 98-99 0.18687906831559786 1101 100 0.20636843875914224 1102 1 0.4749668889411254 1102 2 0.42573596050716844 1102 3 0.47274254170480745 1102 4 0.4491611917194973 1102 5 0.43719867431268256 1102 6 0.3910358569843737 1102 7 0.38892664551504197 1102 8 0.42736987433136164 1102 9 0.39607169192374414 1102 10-11 0.4166344416134393 1102 12-13 0.47129303706832815 1102 14-15 0.5348181655992121 1102 16-17 0.5050182830306653 1102 18-19 0.4732654031814647 1102 20-21 0.4955429113191201 1102 22-23 0.5304072839666674 1102 24-25 0.5985316301108838 1102 26-27 0.6530124620530486 1102 28-29 0.704129620919268 1102 30-31 0.6705590111148325 1102 32-33 0.7192103324683572 1102 34-35 0.6877931756471725 1102 36-37 0.6793258903143595 1102 38-39 0.6956461668413922 1102 40-41 0.6873120875040613 1102 42-43 0.6917357339315231 1102 44-45 0.7450831054175566 1102 46-47 0.7071783727239236 1102 48-49 0.8372384371794723 1102 50-51 0.8116359560803517 1102 52-53 0.7778855227763941 1102 54-55 0.7374537263664536 1102 56-57 0.7599263970134729 1102 58-59 0.6320612826290386 1102 60-61 0.6022318346714499 1102 62-63 0.7203472509169302 1102 64-65 0.6806583501131769 1102 66-67 0.8115430088841435 1102 68-69 0.7956614541445859 1102 70-71 0.7440862246271962 1102 72-73 0.667879544714971 1102 74-75 0.6718284781497843 1102 76-77 0.6283805394169875 1102 78-79 0.6694622642580086 1102 80-81 0.5756449843887239 1102 82-83 0.5834654293641535 1102 84-85 0.6014770623884331 1102 86-87 0.5822961188244236 1102 88-89 0.5848829241095501 1102 90-91 0.5423789129717207 1102 92-93 0.554440307394426 1102 94-95 0.6125376156902504 1102 96-97 0.5958156045565204 1102 98-99 0.7016354632964195 1102 100 0.6896240537806086 1103 1 0.23526434889749126 1103 2 0.2800721288975332 1103 3 0.22576839964227347 1103 4 0.23263239522588464 1103 5 0.21288440563272815 1103 6 0.23837571929897905 1103 7 0.24770941643303246 1103 8 0.3044533933198821 1103 9 0.32876896766338604 1103 10-11 0.323655517307202 1103 12-13 0.2689960116912786 1103 14-15 0.35212562625960686 1103 16-17 0.27608619973270976 1103 18-19 0.3582581254981392 1103 20-21 0.33197969768689006 1103 22-23 0.4191193082713056 1103 24-25 0.39322014924091775 1103 26-27 0.44931755544900653 1103 28-29 0.4225508723626987 1103 30-31 0.36675087531276773 1103 32-33 0.38315713602985824 1103 34-35 0.4130187043054292 1103 36-37 0.3244150387189819 1103 38-39 0.3173570293831105 1103 40-41 0.3932902897724304 1103 42-43 0.4484894310718346 1103 44-45 0.35601474010240963 1103 46-47 0.3449601329760128 1103 48-49 0.3565111413177249 1103 50-51 0.3662934278157337 1103 52-53 0.23759536506246803 1103 54-55 0.35009607857733016 1103 56-57 0.31750780637432996 1103 58-59 0.3361494858587335 1103 60-61 0.47677640934155363 1103 62-63 0.47412204480166764 1103 64-65 0.41748759107992583 1103 66-67 0.36905518494069156 1103 68-69 0.43206743636987 1103 70-71 0.4225389257669079 1103 72-73 0.5616967065751908 1103 74-75 0.5220568428006587 1103 76-77 0.524993580679336 1103 78-79 0.5213781907013626 1103 80-81 0.5131581849110702 1103 82-83 0.4712402312013637 1103 84-85 0.5574721636643574 1103 86-87 0.5298190283821 1103 88-89 0.49746125890357007 1103 90-91 0.4743116986602658 1103 92-93 0.4802734284668695 1103 94-95 0.35976190984384715 1103 96-97 0.3227315017628385 1103 98-99 0.39767272204116466 1103 100 0.517896222062447 1104 1 0.14438011985386012 1104 2 0.218954496213005 1104 3 0.16297337497191222 1104 4 0.2457939532271567 1104 5 0.12520108577184175 1104 6 0.23630882475427484 1104 7 0.21344595815650536 1104 8 0.21927888206272428 1104 9 0.2089484389747014 1104 10-11 0.24408130923040972 1104 12-13 0.3277040592531435 1104 14-15 0.32549028106380007 1104 16-17 0.35401918871800575 1104 18-19 0.37762966233547246 1104 20-21 0.3261558616981546 1104 22-23 0.3420385661155052 1104 24-25 0.4571932007706039 1104 26-27 0.4004161699263591 1104 28-29 0.3754881449802241 1104 30-31 0.4126237048551147 1104 32-33 0.44473493720615664 1104 34-35 0.46434546559501655 1104 36-37 0.507137094934123 1104 38-39 0.486549975153558 1104 40-41 0.44978092771221867 1104 42-43 0.4436755243746049 1104 44-45 0.4004631639385323 1104 46-47 0.37303500258000355 1104 48-49 0.3537093658669619 1104 50-51 0.432522973611853 1104 52-53 0.3844684031309029 1104 54-55 0.3704852720427354 1104 56-57 0.3663689873487712 1104 58-59 0.35447413657752236 1104 60-61 0.4164102321400662 1104 62-63 0.44931539060176817 1104 64-65 0.45967504103421675 1104 66-67 0.44232377439267623 1104 68-69 0.3219218690276975 1104 70-71 0.359561244778682 1104 72-73 0.3410696007359846 1104 74-75 0.33206481875587457 1104 76-77 0.2799648898626117 1104 78-79 0.33272265991061545 1104 80-81 0.23484102410922247 1104 82-83 0.27792836418501565 1104 84-85 0.3138193867171779 1104 86-87 0.23661050609299394 1104 88-89 0.2810924754618327 1104 90-91 0.3101396178184359 1104 92-93 0.3207913929565116 1104 94-95 0.4106813621033538 1104 96-97 0.4174855585585391 1104 98-99 0.4481126314722985 1104 100 0.4394866019987873 1105 1 -0.05248473413941568 1105 2 0.022102702244470862 1105 3 -0.003879106824587808 1105 4 0.00829000256967305 1105 5 -0.04996801978189325 1105 6 0.003355639718975567 1105 7 0.03439563091581732 1105 8 -0.08876182690507761 1105 9 -0.023913978888629117 1105 10-11 0.003008597977974148 1105 12-13 0.06471847664979435 1105 14-15 0.08564325313363241 1105 16-17 0.10805375686216934 1105 18-19 -0.04386359042040766 1105 20-21 0.03938024685395192 1105 22-23 0.08196155985626774 1105 24-25 0.04514256357199997 1105 26-27 0.15949832880069437 1105 28-29 0.1457887562764526 1105 30-31 0.02621642624212228 1105 32-33 0.1286056107464617 1105 34-35 0.0744782599266145 1105 36-37 0.106498089690092 1105 38-39 0.022705782990094292 1105 40-41 0.02641660874462559 1105 42-43 0.1290366732388577 1105 44-45 0.07837389518672921 1105 46-47 0.03975633996136452 1105 48-49 -0.05083262777193909 1105 50-51 0.08962810594037762 1105 52-53 0.11199460371721415 1105 54-55 0.02369731982849288 1105 56-57 -0.037513692707612734 1105 58-59 0.012179921210744737 1105 60-61 0.035866033968986244 1105 62-63 0.06520254066928288 1105 64-65 0.0661105423606898 1105 66-67 0.09776364473275123 1105 68-69 0.1444130600410034 1105 70-71 0.08856714616853623 1105 72-73 0.03251759179945424 1105 74-75 0.03490256623246779 1105 76-77 0.10198178431062388 1105 78-79 0.046101915193176524 1105 80-81 0.0567139317844223 1105 82-83 0.03778443835899736 1105 84-85 -0.004929998373327749 1105 86-87 0.077915533418917 1105 88-89 0.1528354141241266 1105 90-91 0.16163200861268479 1105 92-93 0.12163428695351186 1105 94-95 0.06435344926073583 1105 96-97 0.11276002803856855 1105 98-99 0.10699849817471829 1105 100 0.05201634620514284 1106 1 0.10831897340474583 1106 2 0.13706053235265614 1106 3 0.15135927704415408 1106 4 0.07291154219234386 1106 5 0.1628880782800124 1106 6 0.14598480014489468 1106 7 0.16461802679350512 1106 8 0.16308868913551322 1106 9 0.08592644175833897 1106 10-11 0.08247663618885781 1106 12-13 0.22520033716772048 1106 14-15 0.2665181510693131 1106 16-17 0.16723666615891375 1106 18-19 0.146818666833461 1106 20-21 0.1534136538238826 1106 22-23 0.17218371361816764 1106 24-25 0.11026941554908376 1106 26-27 0.19301091130790837 1106 28-29 0.23174299305233603 1106 30-31 0.19175245390677986 1106 32-33 0.2330779485998491 1106 34-35 0.17964709812146396 1106 36-37 0.17896564199962484 1106 38-39 0.19207078840847913 1106 40-41 0.3166911096103959 1106 42-43 0.2320957645198547 1106 44-45 0.2053124912824913 1106 46-47 0.19660708976892494 1106 48-49 0.2373321261799788 1106 50-51 0.15866450178801017 1106 52-53 0.18067513109550504 1106 54-55 0.21906117811895598 1106 56-57 0.2533994929183905 1106 58-59 0.2521106176526473 1106 60-61 0.2660611709480918 1106 62-63 0.2242064512567552 1106 64-65 0.2402201824096437 1106 66-67 0.2002648260908373 1106 68-69 0.2688643338205381 1106 70-71 0.2541925062114103 1106 72-73 0.23011496826612898 1106 74-75 0.2816166792454382 1106 76-77 0.23145821308600034 1106 78-79 0.32240028199692716 1106 80-81 0.25371207166724474 1106 82-83 0.2823282755102383 1106 84-85 0.2743983640723471 1106 86-87 0.23753203870288786 1106 88-89 0.2817215812975604 1106 90-91 0.24870999661376203 1106 92-93 0.30692383239363963 1106 94-95 0.27134324867197535 1106 96-97 0.22732901183684717 1106 98-99 0.27682394103789676 1106 100 0.1914178526662944 1107 1 0.12707231301594746 1107 2 0.14345221872752134 1107 3 0.18841428508318359 1107 4 0.26085467714977284 1107 5 0.21162184147971175 1107 6 0.10677327186692764 1107 7 0.17187232126394747 1107 8 0.18148141064009593 1107 9 0.16874999766852028 1107 10-11 0.06109294091954354 1107 12-13 0.1295466839026247 1107 14-15 0.21890398129365707 1107 16-17 0.17157306893938085 1107 18-19 0.2146130737784162 1107 20-21 0.2529262802498806 1107 22-23 0.2551240810412878 1107 24-25 0.3130709749528222 1107 26-27 0.3121269057392908 1107 28-29 0.22465403180021326 1107 30-31 0.24194306633456364 1107 32-33 0.20980430295445984 1107 34-35 0.1584808519762504 1107 36-37 0.21397315387845595 1107 38-39 0.25382394351739634 1107 40-41 0.17584577763806664 1107 42-43 0.29679915912232246 1107 44-45 0.21778256956417863 1107 46-47 0.2601208040395804 1107 48-49 0.23363848623738903 1107 50-51 0.21901771584181517 1107 52-53 0.2312850669030766 1107 54-55 0.28907739994341597 1107 56-57 0.27786221986219317 1107 58-59 0.29481690678450434 1107 60-61 0.32951677897897014 1107 62-63 0.32274804454183226 1107 64-65 0.3100974284853777 1107 66-67 0.3430234707418265 1107 68-69 0.3539306707792136 1107 70-71 0.28283828338536665 1107 72-73 0.21945037665116018 1107 74-75 0.1479711297041817 1107 76-77 0.16546330247376773 1107 78-79 0.15260067064979665 1107 80-81 0.1527270211447629 1107 82-83 0.11915636969746402 1107 84-85 0.1496538518173196 1107 86-87 0.1618351831490017 1107 88-89 0.2105072926736895 1107 90-91 0.23347537919243422 1107 92-93 0.1534436879547023 1107 94-95 0.16276185560782608 1107 96-97 0.1280673804053265 1107 98-99 0.14477361744005535 1107 100 0.14436138298577106 1108 1 -0.08382725031615834 1108 2 -0.046796345778780335 1108 3 -0.04456308931517228 1108 4 -0.08148962993044506 1108 5 -0.04500211240436869 1108 6 -0.04507347498972081 1108 7 -0.15618879010792597 1108 8 -0.1402737172406745 1108 9 -0.17824661789773444 1108 10-11 -0.17822867263376452 1108 12-13 -0.08671487341008088 1108 14-15 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54-55 -0.5549178537452022 2214 56-57 -0.5628159439373874 2214 58-59 -0.6295375555066158 2214 60-61 -0.6121465036368079 2214 62-63 -0.5857510592846822 2214 64-65 -0.6188459028223292 2214 66-67 -0.4578995351401858 2214 68-69 -0.5006825826041208 2214 70-71 -0.41338338778857064 2214 72-73 -0.43429113562118715 2214 74-75 -0.49754185881116797 2214 76-77 -0.41672377361986435 2214 78-79 -0.3257147632143287 2214 80-81 -0.3719746303680793 2214 82-83 -0.4591745683998454 2214 84-85 -0.3859118030901314 2214 86-87 -0.3762236292555343 2214 88-89 -0.40846729670413495 2214 90-91 -0.46098754552996013 2214 92-93 -0.4186233959342367 2214 94-95 -0.4557046461283605 2214 96-97 -0.5193334765035615 2214 98-99 -0.558436718843403 2214 100 -0.5893421319150924 2215 1 -0.17429587679874103 2215 2 -0.2113446492936646 2215 3 -0.27445955159086566 2215 4 -0.19741598926167114 2215 5 -0.19968281547016176 2215 6 -0.151128229404641 2215 7 -0.09468314010132417 2215 8 -0.24128833057969956 2215 9 -0.3095959602010794 2215 10-11 -0.18783739169369085 2215 12-13 -0.2604849458019487 2215 14-15 -0.2406801838006416 2215 16-17 -0.27108646964976657 2215 18-19 -0.28141543853164563 2215 20-21 -0.2550349255072746 2215 22-23 -0.2042385849690831 2215 24-25 -0.288119468683405 2215 26-27 -0.26845455214397873 2215 28-29 -0.3277109038921324 2215 30-31 -0.2559444886983826 2215 32-33 -0.26669947529123306 2215 34-35 -0.31390331676412586 2215 36-37 -0.29914041225679 2215 38-39 -0.24952999014225696 2215 40-41 -0.3141803829533245 2215 42-43 -0.34541054490750156 2215 44-45 -0.34872321402301765 2215 46-47 -0.29138730392070755 2215 48-49 -0.24708288979148563 2215 50-51 -0.31185952991279464 2215 52-53 -0.3918488261028372 2215 54-55 -0.3163470488155511 2215 56-57 -0.27268195689953956 2215 58-59 -0.3326922767680571 2215 60-61 -0.27611048272721916 2215 62-63 -0.2026609995483355 2215 64-65 -0.2129056615635676 2215 66-67 -0.34936033339099737 2215 68-69 -0.38007354043793384 2215 70-71 -0.31967249532520015 2215 72-73 -0.28759566416665194 2215 74-75 -0.30530441333318237 2215 76-77 -0.30438957838160263 2215 78-79 -0.28748200411114766 2215 80-81 -0.34790602870619836 2215 82-83 -0.34890036323662343 2215 84-85 -0.3033491470797429 2215 86-87 -0.28498726209149794 2215 88-89 -0.28125348831567365 2215 90-91 -0.2434481534900783 2215 92-93 -0.2646381117481411 2215 94-95 -0.24759527748304322 2215 96-97 -0.3291769157653839 2215 98-99 -0.39014120112211614 2215 100 -0.3353536762173803 2216 1 0.13750973512863496 2216 2 0.10534880526163093 2216 3 0.06487968048965698 2216 4 0.1517790211558463 2216 5 0.1006543331079186 2216 6 0.1682662019615364 2216 7 0.07009213927741342 2216 8 0.08270658242730633 2216 9 0.09887496546929953 2216 10-11 0.06996356617626986 2216 12-13 0.04935919739149597 2216 14-15 -0.003090282022903068 2216 16-17 0.08686195690000886 2216 18-19 0.026397154481863083 2216 20-21 0.04696848386036834 2216 22-23 0.07794341032273877 2216 24-25 0.014943821085786624 2216 26-27 -0.055855856362391876 2216 28-29 -0.07083480464165604 2216 30-31 -0.026289213649000942 2216 32-33 -0.08780021398755622 2216 34-35 -0.10896054543528066 2216 36-37 -0.07408751294137517 2216 38-39 -0.1253708322019449 2216 40-41 -0.0010946346512454852 2216 42-43 0.003989298153356913 2216 44-45 0.07141965471632972 2216 46-47 0.10534376191255745 2216 48-49 0.09067748036842715 2216 50-51 0.08246522588449068 2216 52-53 0.09419197807499557 2216 54-55 0.1669286499695275 2216 56-57 0.16609067074880812 2216 58-59 0.16591745887066622 2216 60-61 0.11134764428526012 2216 62-63 0.10699806368732112 2216 64-65 0.14096099427970898 2216 66-67 0.09108288440265255 2216 68-69 0.0951942031559696 2216 70-71 0.07592265579636859 2216 72-73 0.03874359497234536 2216 74-75 0.01420664005318173 2216 76-77 0.034410648909570796 2216 78-79 0.06256210120042027 2216 80-81 0.17672069965378512 2216 82-83 0.2602800356052768 2216 84-85 0.17443855213826254 2216 86-87 0.1921123401489453 2216 88-89 0.256292849319955 2216 90-91 0.1650831125758927 2216 92-93 0.17601913856979579 2216 94-95 0.24403499553694985 2216 96-97 0.18642581172873207 2216 98-99 0.24560926563655272 2216 100 0.3169985168718874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23440.0 3 3527.0 4 2260.0 5 2117.0 6 1567.0 7 1718.0 8 2031.0 9 2248.0 10 2401.0 11 2408.0 12 2678.0 13 2979.0 14 3255.0 15 3358.0 16 3413.0 17 3257.0 18 3270.0 19 3088.0 20 3071.0 21 3207.0 22 3893.0 23 5554.0 24 8740.0 25 11774.0 26 12247.0 27 10221.0 28 10362.0 29 13468.0 30 15951.0 31 19178.0 32 24594.0 33 33471.0 34 48429.0 35 76333.0 36 146377.0 37 309909.0 38 487965.0 39 218680.0 40 477.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66343659845941 19.483167325280252 13.292743673425154 23.560652402835185 2 15.71888559121196 19.83354638386439 40.21706345423897 24.230504570684683 3 18.358662455229542 25.293170603681396 29.617771603092507 26.730395337996555 4 12.701309232264842 16.656688392535944 38.325256045561 32.31674632963822 5 13.12945871006788 37.856411173779605 35.02346749642389 13.990662619728628 6 30.987462165825324 37.837373766390456 17.70051786138655 13.474646206397663 7 27.941959913821247 32.587386563948556 21.68329307305608 17.78736044917412 8 25.533362320770802 36.4641724413037 19.91143162686117 18.091033611064322 9 25.044933645752472 17.724389127657254 20.843205242791846 36.38747198379843 10-11 24.12569881093602 27.232783772896813 28.078473130340033 20.56304428582714 12-13 24.67798655049733 24.562920541779736 28.165547506919463 22.59354540080347 14-15 22.097391236224997 26.50813443359273 25.237700423042774 26.156773907139502 16-17 21.891401471295314 29.865329833699956 25.79512912922519 22.44813956577954 18-19 21.80202364685998 28.740087570807727 27.720266955827466 21.737621826504824 20-21 23.02606754189543 27.993164859412843 27.27824962951841 21.70251796917332 22-23 22.702218363190056 27.782975139708572 27.29092677704079 22.223879720060584 24-25 22.44810158728501 27.748321953770372 27.348358904571946 22.45521755437268 26-27 22.213909447772817 27.722243221770242 27.47736455454736 22.58648277590958 28-29 22.321173858576337 27.809649460092682 27.51621092844735 22.35296575288363 30-31 22.41405367893244 28.19722207623867 27.163799966770153 22.224924278058737 32-33 22.122367441799735 27.924767264228546 27.596717544294876 22.356147749676847 34-35 22.34361703968046 27.593141381404983 27.597482609789832 22.465758969124728 36-37 22.679135046919622 28.023827009383922 27.04763769889841 22.249400244798043 38-39 22.391610669579048 28.147133156923605 27.227493805694486 22.233762367802864 40-41 22.20114669578288 27.782385669077243 27.590649516824246 22.425818118315632 42-43 22.120868972815792 27.849899116448768 27.52268451766368 22.50654739307176 44-45 22.282663337907767 27.872915227635666 27.540801017430283 22.303620417026288 46-47 22.244141141445038 28.178147167606284 27.32119831163023 22.25651337931845 48-49 22.163373222900052 27.898245205106935 27.502548799346567 22.435832772646442 50-51 22.167359031070703 27.77542908909873 27.4894186587446 22.567793221085967 52-53 22.24117127799048 27.72699909636509 27.484669546458846 22.54716007918558 54-55 22.273868099954033 28.175078872592035 27.3201143391417 22.230938688312232 56-57 22.119731121910835 27.925596597336096 27.380717258516864 22.57395502223621 58-59 22.08208534982984 27.45208967526585 27.72970105524316 22.736123919661146 60-61 22.431786784081144 27.89615634719796 27.25962806561213 22.412428803108767 62-63 22.167793222904052 27.83521005654653 27.379527688604316 22.6174690319451 64-65 22.18990830959028 27.612726190134207 27.53085508945756 22.666510410817953 66-67 22.00277538479819 27.784467964740667 27.375994782198234 22.83676186826291 68-69 22.45949818191077 27.908901961590804 27.25380878186553 22.377791074632896 70-71 22.40912570366272 27.61609108729253 27.399145077339654 22.575638131705094 72-73 22.099078160499307 27.798096257801685 27.563327501109864 22.53949808058914 74-75 22.201892960725715 27.73417595492671 27.512628714963988 22.55130236938359 76-77 22.28750088135983 27.760549550702347 27.276128075292938 22.67582149264489 78-79 22.339508403442267 27.87663700400235 27.335808323107923 22.448046269447456 80-81 22.210680976650522 27.723167229710434 27.427907928716017 22.638243864923034 82-83 22.179300151170274 27.690241634140524 27.51240193599795 22.61805627869126 84-85 22.199292433583224 27.713257371245177 27.44744664362952 22.640003551542083 86-87 22.368920147886133 27.719607839296895 27.42255596489957 22.488916047917403 88-89 22.15378950694497 27.70744818365327 27.40540921661152 22.733353092790235 90-91 22.10987385371261 27.59354560309389 27.49385850438016 22.80272203881334 92-93 22.313766874385728 27.763576950332503 27.30832718314737 22.6143289921344 94-95 22.08834869440669 27.55422726554615 27.447457034039825 22.909967006007335 96-97 22.187376065934963 27.494585662409726 27.503725162185322 22.814313109469992 98-99 22.13507810166681 27.570908414350924 27.471712524501125 22.82230095948114 100 22.312281063663388 27.48234473880397 27.304388584171434 22.900985613361204 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1704.0 1 1136.5 2 499.0 3 379.5 4 291.5 5 244.0 6 214.5 7 190.5 8 205.5 9 218.5 10 202.5 11 199.5 12 197.5 13 206.0 14 239.0 15 264.0 16 314.5 17 423.0 18 560.0 19 765.5 20 1085.0 21 1526.0 22 2200.0 23 3436.0 24 5175.5 25 7041.0 26 9428.0 27 12640.5 28 16537.5 29 20468.5 30 24034.5 31 27490.5 32 30693.0 33 34035.5 34 37181.0 35 39986.0 36 42955.0 37 45543.0 38 47570.0 39 48688.0 40 49890.5 41 51244.0 42 51725.0 43 52543.5 44 52788.5 45 52689.0 46 52966.5 47 51865.0 48 50930.5 49 50463.0 50 49730.0 51 48452.5 52 45117.0 53 42516.5 54 41523.5 55 39609.5 56 36711.0 57 33314.0 58 30293.5 59 28418.0 60 26312.5 61 23090.0 62 20063.0 63 17620.5 64 14714.0 65 11777.5 66 9544.0 67 7503.0 68 5768.0 69 4460.0 70 3344.0 71 2552.0 72 1997.0 73 1482.5 74 1157.0 75 924.5 76 665.5 77 477.0 78 356.5 79 249.0 80 177.5 81 124.5 82 84.0 83 66.0 84 56.5 85 51.0 86 43.5 87 32.5 88 25.0 89 22.5 90 18.0 91 13.0 92 10.5 93 8.5 94 5.5 95 3.0 96 1.5 97 1.0 98 1.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07586847550681185 2 0.07815170563814325 3 0.07919546798389475 4 0.08187010899488295 5 0.07978258430337996 6 0.08036970062286518 7 0.07932593827711369 8 0.07945640857033262 9 0.07913023283728528 10-11 0.07873882195762846 12-13 0.07740150145213437 14-15 0.07541182948054558 16-17 0.07413974412166094 18-19 0.07352001022887099 20-21 0.07345477508226152 22-23 0.07541182948054558 24-25 0.07498780102758404 26-27 0.07417236169496567 28-29 0.0703887231916165 30-31 0.07570538764028818 32-33 0.0741071265483562 34-35 0.07120416252423485 36-37 0.0679097876204567 38-39 0.06950804871238868 40-41 0.07329168721573784 42-43 0.07824955835805746 44-45 0.07919546798389475 46-47 0.08226151987453977 48-49 0.0856211299249274 50-51 0.08666489227067889 52-53 0.08637133411093628 54-55 0.08689321528381203 56-57 0.08431642699273802 58-59 0.08340313494020546 60-61 0.08141346296861668 62-63 0.0820005792881019 64-65 0.08089158179574092 66-67 0.08066325878260779 68-69 0.07994567216990364 70-71 0.07818432321144798 72-73 0.07802123534492432 74-75 0.0775319717453533 76-77 0.07776029475848643 78-79 0.07779291233179118 80-81 0.07903238011737107 82-83 0.07824955835805746 84-85 0.0774341190254391 86-87 0.07808647049153378 88-89 0.07567277006698345 90-91 0.07325906964243312 92-93 0.07303074662929998 94-95 0.07319383449582365 96-97 0.07198698428354848 98-99 0.07166080855050114 100 0.07032348804500703 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1532916.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.99936967393265 #Duplication Level Percentage of deduplicated Percentage of total 1 82.9570331925354 58.898970666895224 2 10.928589711472627 15.518459618791642 3 2.8521277811412697 6.074978240716268 4 1.1481855950400626 3.2608181406613492 5 0.577765207172622 2.051048276439264 6 0.34547336260946093 1.4717034590643419 7 0.23021347876954726 1.1441508318156792 8 0.168461446661582 0.9568525221864919 9 0.13056551881669254 0.8343062583421647 >10 0.6286358894700795 7.739144110254038 >50 0.02661465369792975 1.274263385519641 >100 0.006150591357488399 0.6489452554507545 >500 1.8357125521232542E-4 0.1263592338631273 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 6.523514660946848E-5 0.0 0.0 0.0 14-15 0.0 6.523514660946848E-5 0.0 0.0 0.0 16-17 0.0 6.523514660946848E-5 0.0 0.0 0.0 18-19 0.0 6.523514660946848E-5 0.0 6.523514660946848E-5 0.0 20-21 0.0 6.523514660946848E-5 0.0 9.785271991420273E-5 0.0 22-23 0.0 6.523514660946848E-5 0.0 1.9570543982840545E-4 0.0 24-25 0.0 6.523514660946848E-5 0.0 2.6094058643787393E-4 0.0 26-27 0.0 6.523514660946848E-5 0.0 3.2617573304734245E-4 0.0 28-29 0.0 6.523514660946848E-5 0.0 0.0012394677855799012 0.0 30-31 0.0 6.523514660946848E-5 0.0 0.005349282021976416 0.0 32-33 0.0 6.523514660946848E-5 0.0 0.014123409240949927 0.0 34-35 0.0 6.523514660946848E-5 0.0 0.025180766591254837 0.0 36-37 0.0 6.523514660946848E-5 0.0 0.045827690493151614 0.0 38-39 0.0 6.523514660946848E-5 0.0 0.081250375102093 0.0 40-41 0.0 6.523514660946848E-5 0.0 0.12769779948803456 0.0 42-43 0.0 6.523514660946848E-5 0.0 0.1743083117404998 0.0 44-45 0.0 6.523514660946848E-5 0.0 0.22799683740009236 0.0 46-47 0.0 6.523514660946848E-5 0.0 0.2923186919570283 0.0 48-49 0.0 6.523514660946848E-5 0.0 0.37027469215534314 0.0 50-51 0.0 6.523514660946848E-5 0.0 0.4563198505332321 0.0 52-53 0.0 6.523514660946848E-5 0.0 0.5386466055543813 0.0 54-55 0.0 6.523514660946848E-5 0.0 0.6316719246194834 0.0 56-57 0.0 6.523514660946848E-5 0.0 0.739962267991201 0.0 58-59 0.0 6.523514660946848E-5 0.0 0.8640068992691055 0.0 60-61 0.0 6.523514660946848E-5 0.0 1.004099376612939 0.0 62-63 0.0 6.523514660946848E-5 0.0 1.143637355210592 0.0 64-65 0.0 6.523514660946848E-5 0.0 1.2941674560119405 0.0 66-67 0.0 6.523514660946848E-5 0.0 1.4623762815444552 0.0 68-69 0.0 6.523514660946848E-5 0.0 1.6541676125762925 0.0 70-71 0.0 6.523514660946848E-5 0.0 1.8714985035057368 0.0 72-73 0.0 6.523514660946848E-5 0.0 2.1005391032515806 0.0 74-75 0.0 6.523514660946848E-5 0.0 2.324393508841972 0.0 76-77 0.0 6.523514660946848E-5 0.0 2.5542169303471294 0.0 78-79 0.0 6.523514660946848E-5 0.0 2.8209960624065507 0.0 80-81 0.0 6.523514660946848E-5 0.0 3.1196099460113924 0.0 82-83 0.0 6.523514660946848E-5 0.0 3.4298683032860247 0.0 84-85 0.0 6.523514660946848E-5 0.0 3.7354949651513847 0.0 86-87 0.0 6.523514660946848E-5 0.0 4.054494832071685 0.0 88 0.0 6.523514660946848E-5 0.0 4.311521309712991 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACGC 2315 0.0 35.329002 4 GTATCAA 2525 0.0 34.623466 1 CAACGCA 2390 0.0 34.415897 5 ATCAACG 2410 0.0 33.936367 3 AACGCAG 2465 0.0 33.178085 6 GGTATCA 1445 0.0 33.17808 1 TATCAAC 2920 0.0 28.652079 2 GTACATG 4920 0.0 27.9912 1 TACATGG 5050 0.0 26.43297 2 ACGCAGA 3060 0.0 26.419586 7 ACATGGG 5140 0.0 25.239407 3 CGCAGAG 3175 0.0 25.165756 8 GAGTACA 3830 0.0 20.494467 1 GCAGAGT 3660 0.0 20.289942 9 CATGGGG 3755 0.0 19.777906 4 AGTACAT 3830 0.0 19.144533 2 CCGGTCG 180 1.3377021E-4 18.27807 9 TACACTG 1190 0.0 17.378996 5 CATACAC 845 0.0 17.244005 3 CTTACAC 640 0.0 16.891998 3 >>END_MODULE