FastQCFastQC Report
Wed 25 May 2016
SRR1032995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532916
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44190.28827411286724125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38280.24972014122104538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19550.1275347116215109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24500.054.653431
GTATCAA47250.042.0607721
TCAACGC57000.034.5793274
ATCAACG56950.034.4444853
CAACGCA58000.033.8987055
AACGCAG60150.032.765236
TATCAAC60700.032.704022
GTGGTAT10200.030.8612351
ACGCAGA68200.028.6219067
TATAACG1354.6011155E-727.8762042
CGCAGAG70700.027.5423858
GTACATG51000.027.176311
GCAGAGT74950.025.5723449
TACATGG51800.025.4276182
TGGTATC11500.024.5431792
ACATGGG52950.023.4539153
AGAGTAC69850.021.6892910-11
CAGAGTA74200.021.24078810-11
GTACACG1605.429674E-520.5549621
GAGTACT51600.020.30258612-13
Produced by FastQC (version 0.11.5)