FastQCFastQC Report
Wed 25 May 2016
SRR1032994_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032994_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2893420
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36900.031.3339861
TCAACGC35550.031.0700994
GGTATCA25950.030.9717431
CAACGCA37950.029.2290445
ATCAACG37850.029.1820893
GTACATG86250.029.1542281
AACGCAG40250.027.208496
TACATGG87650.026.9730762
ACATGGG88100.025.7149413
GTATACG2451.2732926E-1124.9392951
TATCAAC45700.024.889352
TATAGCG1201.8385096E-423.5008957
GAGTACA62200.022.1403311
ACGCAGA49550.021.8171797
CATGGGG58500.021.291414
CGCAGAG51450.020.7367128
AGTACAT63350.019.8097532
GTCTAGG10050.019.1745281
ACCGTCC3004.2455213E-918.8000668
TATACCG1851.6502917E-417.7844625
Produced by FastQC (version 0.11.5)