FastQCFastQC Report
Wed 25 May 2016
SRR1032994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2893420
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65150.22516606645423065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54490.1883238520505146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29410.10164442078923903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44550.050.8321381
GTATCAA78900.042.6358641
TCAACGC92400.035.8478624
ATCAACG93500.035.37583
CAACGCA94700.034.9735835
AACGCAG98500.033.672116
TATCAAC100050.033.2473752
TACACCG4650.029.3401625
ACGCAGA115500.028.5094227
CGCAGAG117750.027.9247068
GTACATG89250.026.4788321
TACATGG89950.025.8916132
GTGGTAT20850.025.6884481
GCAGAGT129250.025.1864369
ACATGGG91100.024.738863
TGGTATC21200.022.6370262
GAGTACT88350.021.98495112-13
GAGTACA63750.020.9303841
CAGAGTA128950.020.8003810-11
CCGCGCA1857.2198127E-620.3133839
Produced by FastQC (version 0.11.5)