##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032994_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2893420
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.814454866559295	34.0	31.0	34.0	31.0	34.0
2	32.867652466631185	34.0	31.0	34.0	31.0	34.0
3	32.94710515583635	34.0	31.0	34.0	31.0	34.0
4	36.35386946934769	37.0	37.0	37.0	35.0	37.0
5	36.33676306930899	37.0	37.0	37.0	35.0	37.0
6	36.39757518784	37.0	37.0	37.0	35.0	37.0
7	36.36079518355441	37.0	37.0	37.0	35.0	37.0
8	36.303628232334056	37.0	37.0	37.0	35.0	37.0
9	38.14203641365581	39.0	39.0	39.0	37.0	39.0
10-11	38.151253188268555	39.0	39.0	39.0	37.0	39.0
12-13	38.14077061055775	39.0	39.0	39.0	37.0	39.0
14-15	39.68712613447063	41.0	40.0	41.0	37.0	41.0
16-17	39.7077398027248	41.0	40.0	41.0	37.0	41.0
18-19	39.73128823330177	41.0	40.0	41.0	37.0	41.0
20-21	39.74521189457458	41.0	40.0	41.0	37.0	41.0
22-23	39.70750547794651	41.0	40.0	41.0	37.0	41.0
24-25	39.691475831369104	41.0	40.0	41.0	37.0	41.0
26-27	39.61283809471145	41.0	40.0	41.0	37.0	41.0
28-29	39.553860829053505	41.0	40.0	41.0	37.0	41.0
30-31	39.44406809243041	41.0	39.5	41.0	36.0	41.0
32-33	39.31776720973796	41.0	39.0	41.0	35.5	41.0
34-35	39.228060392200234	41.0	39.0	41.0	35.0	41.0
36-37	39.13847626683993	41.0	39.0	41.0	35.0	41.0
38-39	39.040311292518886	41.0	39.0	41.0	35.0	41.0
40-41	38.94012448935861	41.0	39.0	41.0	35.0	41.0
42-43	38.80126511187453	41.0	39.0	41.0	35.0	41.0
44-45	38.6300139627154	41.0	39.0	41.0	35.0	41.0
46-47	38.407375182310204	41.0	38.0	41.0	35.0	41.0
48-49	38.1996163709382	41.0	38.0	41.0	34.0	41.0
50-51	38.04454209896939	40.0	38.0	41.0	34.0	41.0
52-53	37.906329706713855	40.0	38.0	41.0	34.0	41.0
54-55	37.75967851193397	40.0	37.5	41.0	33.5	41.0
56-57	37.55549747357799	40.0	37.0	41.0	33.0	41.0
58-59	37.387625543474506	40.0	36.5	41.0	33.0	41.0
60-61	37.203770105964566	40.0	36.0	41.0	33.0	41.0
62-63	36.973227875662715	39.0	35.5	41.0	33.0	41.0
64-65	36.68821826765557	39.0	35.0	41.0	32.0	41.0
66-67	36.41849403128478	39.0	35.0	41.0	32.0	41.0
68-69	36.13072419489739	38.0	35.0	40.5	32.0	41.0
70-71	35.81391761306689	37.0	35.0	40.0	31.0	41.0
72-73	35.47304262775539	37.0	35.0	39.5	31.0	41.0
74-75	35.125992251384176	36.0	35.0	39.0	31.0	41.0
76-77	34.77517678041902	36.0	35.0	39.0	31.0	40.5
78-79	34.42908789598468	35.0	35.0	37.5	30.5	39.5
80-81	34.096170103199675	35.0	34.0	37.0	30.0	39.0
82-83	33.783350153106014	35.0	34.0	36.5	30.0	39.0
84-85	33.50259848207312	35.0	34.0	36.0	30.0	37.5
86-87	33.351619709547876	35.0	34.0	36.0	30.0	37.0
88-89	33.15956238638013	35.0	34.0	36.0	30.0	37.0
90-91	32.97946219352876	35.0	34.0	35.0	30.0	36.0
92-93	32.81439265644117	35.0	34.0	35.0	29.0	36.0
94-95	32.71362263342343	35.0	34.0	35.0	29.5	36.0
96-97	32.648249130786404	35.0	34.0	35.0	30.0	36.0
98-99	32.532771080589754	35.0	34.0	35.0	29.0	36.0
100	32.4485321868239	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17184966267048196
1101	2	0.2123762200150665
1101	3	0.16853400716183842
1101	4	0.08493679068677551
1101	5	0.13174105844534267
1101	6	0.1282278098515377
1101	7	0.06914930251600282
1101	8	-0.0010865399188588754
1101	9	0.06363470590125075
1101	10-11	0.16020679957389206
1101	12-13	0.22984695668863253
1101	14-15	0.2811588702712058
1101	16-17	0.27799003590844507
1101	18-19	0.264481300678824
1101	20-21	0.32658503430700847
1101	22-23	0.2763753867331502
1101	24-25	0.24032467757832165
1101	26-27	0.3083808613082013
1101	28-29	0.31613137387235923
1101	30-31	0.24340453564425246
1101	32-33	0.23007901444321277
1101	34-35	0.2498054405157859
1101	36-37	0.249057848757694
1101	38-39	0.25662082979427936
1101	40-41	0.22181917592295264
1101	42-43	0.23850201359454104
1101	44-45	0.18059163975157588
1101	46-47	0.12887391799525005
1101	48-49	0.06445095448270877
1101	50-51	0.08477110377324237
1101	52-53	0.06325914395782206
1101	54-55	0.07051066129605488
1101	56-57	0.07841652961505474
1101	58-59	0.113474060951404
1101	60-61	0.07832954798792002
1101	62-63	0.06139998413713954
1101	64-65	0.13026313418647106
1101	66-67	0.1299698306337831
1101	68-69	0.10327712451512383
1101	70-71	0.13210025387126478
1101	72-73	0.12799370618945716
1101	74-75	0.17225078374363534
1101	76-77	0.12713492901768575
1101	78-79	0.10354813487342085
1101	80-81	0.12731917379664992
1101	82-83	0.1352103240430651
1101	84-85	0.14254261235664956
1101	86-87	0.1883097729411034
1101	88-89	0.14665932350130362
1101	90-91	0.1505858416926742
1101	92-93	0.219901265060372
1101	94-95	0.20827186444681445
1101	96-97	0.1987673289693319
1101	98-99	0.17299623606383818
1101	100	0.17494401500830037
1102	1	0.18136039593014175
1102	2	0.19581023601934788
1102	3	0.18291652882486176
1102	4	0.14286040379284515
1102	5	0.10769944072503534
1102	6	0.16650299976537042
1102	7	0.14775785763900728
1102	8	-0.018112083546938607
1102	9	0.05351448575328277
1102	10-11	0.1634768433976319
1102	12-13	0.19187188202975136
1102	14-15	0.22623376969119846
1102	16-17	0.24182552842241023
1102	18-19	0.2500689565290273
1102	20-21	0.2749988137169481
1102	22-23	0.2719749891782186
1102	24-25	0.26930229167899
1102	26-27	0.2577436150625303
1102	28-29	0.3024677859944873
1102	30-31	0.3207960399706593
1102	32-33	0.2992187507524591
1102	34-35	0.28444140703454934
1102	36-37	0.32904987393139606
1102	38-39	0.3209494982388179
1102	40-41	0.31074194402597044
1102	42-43	0.30307206492116023
1102	44-45	0.2792017619403353
1102	46-47	0.2964168116942787
1102	48-49	0.2800760732430163
1102	50-51	0.19341608535272314
1102	52-53	0.25270438899208614
1102	54-55	0.2688058840987111
1102	56-57	0.21106669007306778
1102	58-59	0.18192647857078015
1102	60-61	0.2146022768784448
1102	62-63	0.159271681997474
1102	64-65	0.19992471512506427
1102	66-67	0.2716071848922681
1102	68-69	0.22564889157014534
1102	70-71	0.2127196268324596
1102	72-73	0.23788122619903618
1102	74-75	0.1798372658589784
1102	76-77	0.2654318062350285
1102	78-79	0.2560139525998153
1102	80-81	0.22728372577847722
1102	82-83	0.21716818887816913
1102	84-85	0.2801769414742239
1102	86-87	0.268957240966337
1102	88-89	0.21226211287834218
1102	90-91	0.17432108632474552
1102	92-93	0.20895745538402366
1102	94-95	0.1820180700650127
1102	96-97	0.19910160156432966
1102	98-99	0.22035467100059236
1102	100	0.2481787209778048
1103	1	0.14028749784752392
1103	2	0.15149557528383895
1103	3	0.13233702816668114
1103	4	0.07676248143508246
1103	5	0.1113805094129745
1103	6	0.07858348497598655
1103	7	0.07057696256341472
1103	8	0.040743937817431686
1103	9	0.07195441432063632
1103	10-11	0.058070051752544316
1103	12-13	0.10973938303452968
1103	14-15	0.12604905139830436
1103	16-17	0.12892422076747323
1103	18-19	0.14906718020190368
1103	20-21	0.15127339116347116
1103	22-23	0.14531529322519532
1103	24-25	0.08152561846113571
1103	26-27	0.14248958230555075
1103	28-29	0.14735611036752516
1103	30-31	0.09513697791183517
1103	32-33	0.10909696865200402
1103	34-35	0.1558069876907524
1103	36-37	0.09240631936935984
1103	38-39	0.08580112616827762
1103	40-41	0.05947100301209218
1103	42-43	0.10764471198068293
1103	44-45	0.06388746116483901
1103	46-47	0.10774198643345301
1103	48-49	0.11371982994700858
1103	50-51	0.12287581571607831
1103	52-53	0.0643386448993084
1103	54-55	0.1027302860038688
1103	56-57	0.18626088181925837
1103	58-59	0.14162778168095258
1103	60-61	0.03389757556097095
1103	62-63	0.049620158997058184
1103	64-65	0.16120797568604672
1103	66-67	0.14877055017751672
1103	68-69	0.21936941227483686
1103	70-71	0.1801126248798326
1103	72-73	0.15904889127715904
1103	74-75	0.1739305755345839
1103	76-77	0.1023061197608115
1103	78-79	0.10368385141521941
1103	80-81	0.1009648556458842
1103	82-83	0.04609073230091809
1103	84-85	0.10533026783309651
1103	86-87	0.08465927598272316
1103	88-89	0.06842571951035126
1103	90-91	0.1140287520995642
1103	92-93	0.10735521506683199
1103	94-95	0.10072182572270094
1103	96-97	0.09994229485005945
1103	98-99	0.06571651196818351
1103	100	0.04185283892907421
1104	1	0.10224377511799787
1104	2	0.12849840916445743
1104	3	0.15388914008247667
1104	4	0.05921378816891831
1104	5	0.06633080404659353
1104	6	0.060033937047414554
1104	7	0.10189021882719373
1104	8	0.002295374999739863
1104	9	0.10620838400261334
1104	10-11	0.07122454322841776
1104	12-13	0.11748194972069115
1104	14-15	0.16604668983112703
1104	16-17	0.18469656569122606
1104	18-19	0.18538189838822206
1104	20-21	0.17819525702645223
1104	22-23	0.1982312664157746
1104	24-25	0.2118082193337898
1104	26-27	0.23823988593560586
1104	28-29	0.24247947413883963
1104	30-31	0.18379976860951075
1104	32-33	0.18325580091325833
1104	34-35	0.20092135501565878
1104	36-37	0.17132764544556522
1104	38-39	0.1724425278146242
1104	40-41	0.15552493670828227
1104	42-43	0.14815964608170873
1104	44-45	0.15143975300956214
1104	46-47	0.14723919753506465
1104	48-49	0.14559536411100993
1104	50-51	0.18928926990167838
1104	52-53	0.17064852749568615
1104	54-55	0.13273905753910498
1104	56-57	0.17797852834888062
1104	58-59	0.19580888014706233
1104	60-61	0.136808488250459
1104	62-63	0.1867990983160226
1104	64-65	0.1342439389805392
1104	66-67	0.1858612118661469
1104	68-69	0.2514136298089156
1104	70-71	0.23948073697555827
1104	72-73	0.2325076875526335
1104	74-75	0.2622447539341195
1104	76-77	0.2255068304800858
1104	78-79	0.19534639471062576
1104	80-81	0.18477799945408435
1104	82-83	0.18071411856334407
1104	84-85	0.1769223014798058
1104	86-87	0.1838422861936877
1104	88-89	0.2296139251691116
1104	90-91	0.20957382429604365
1104	92-93	0.16842421781074535
1104	94-95	0.10668478285463578
1104	96-97	0.14608844212188643
1104	98-99	0.20892307037761526
1104	100	0.1947586911174568
1105	1	0.07213161428234116
1105	2	0.1369018430200768
1105	3	0.090182399801634
1105	4	0.08908244374375585
1105	5	0.05756024272238136
1105	6	0.06328268724933395
1105	7	0.06580421182565743
1105	8	0.013724878012006059
1105	9	0.05636418595121029
1105	10-11	0.05090197843059485
1105	12-13	0.0700583653876734
1105	14-15	0.12936296456533825
1105	16-17	0.1414306913111858
1105	18-19	0.1181971977207823
1105	20-21	0.08550770907898908
1105	22-23	0.11068712713237971
1105	24-25	0.10621837285312807
1105	26-27	0.08601837933007772
1105	28-29	0.06322868224339118
1105	30-31	0.09114590181729909
1105	32-33	0.18802379844288453
1105	34-35	0.2193848696094065
1105	36-37	0.18129772760571683
1105	38-39	0.1912644547701774
1105	40-41	0.23216662121934206
1105	42-43	0.1902648580722257
1105	44-45	0.15817730714676514
1105	46-47	0.2296737243605662
1105	48-49	0.29905930554365767
1105	50-51	0.31091893964573103
1105	52-53	0.3046554033985487
1105	54-55	0.29997977074433635
1105	56-57	0.3334412493214245
1105	58-59	0.24820686335054631
1105	60-61	0.19651689285783647
1105	62-63	0.24573870394910813
1105	64-65	0.20272911766151225
1105	66-67	0.24575806987168392
1105	68-69	0.2335528381941927
1105	70-71	0.17630832190499035
1105	72-73	0.21204021612782498
1105	74-75	0.21109895612274698
1105	76-77	0.2043453431754898
1105	78-79	0.23810966917010035
1105	80-81	0.2708785373861389
1105	82-83	0.2621260728595942
1105	84-85	0.2098436317352963
1105	86-87	0.2321427598352912
1105	88-89	0.20611126680267944
1105	90-91	0.16845310298203486
1105	92-93	0.17952910466738103
1105	94-95	0.17950220372607362
1105	96-97	0.1513886651826013
1105	98-99	0.13256510142203126
1105	100	0.19176449106416982
1106	1	0.03512693671216027
1106	2	0.12347307107904015
1106	3	0.07328310277684125
1106	4	0.05804908632943295
1106	5	0.06501891492174394
1106	6	0.06502810141824256
1106	7	0.0754691709664641
1106	8	0.0885933492607407
1106	9	0.12020893329135873
1106	10-11	0.09212804090792304
1106	12-13	0.037897373008995316
1106	14-15	0.03361384466425221
1106	16-17	0.05702351831575214
1106	18-19	0.10923700003144177
1106	20-21	0.13257678180330146
1106	22-23	0.18899713862812462
1106	24-25	0.12921233507587004
1106	26-27	0.12382045888255533
1106	28-29	0.11544560065653542
1106	30-31	0.1974848883864766
1106	32-33	0.17067103434067832
1106	34-35	0.15186190488165607
1106	36-37	0.18078032746736028
1106	38-39	0.17936968797829422
1106	40-41	0.22392666205218603
1106	42-43	0.2022502406168769
1106	44-45	0.15611552833873787
1106	46-47	0.17980172084488544
1106	48-49	0.19099393217208416
1106	50-51	0.23164355279800475
1106	52-53	0.2117266329275509
1106	54-55	0.1550058274191457
1106	56-57	0.18030381687204056
1106	58-59	0.1820614411269048
1106	60-61	0.14665501323249686
1106	62-63	0.14520586557137705
1106	64-65	0.1210985810792451
1106	66-67	0.16421337833220662
1106	68-69	0.161109579548544
1106	70-71	0.16791414676434613
1106	72-73	0.10769763299806812
1106	74-75	0.1318599598069028
1106	76-77	0.13089449394196606
1106	78-79	0.06276080961664121
1106	80-81	0.1325643387602895
1106	82-83	0.1741116329190362
1106	84-85	0.20626970428658353
1106	86-87	0.22741454246710902
1106	88-89	0.1417282335997001
1106	90-91	0.14851846947490088
1106	92-93	0.1525961447364068
1106	94-95	0.17615405290040798
1106	96-97	0.15072667370075266
1106	98-99	0.14749839441513046
1106	100	0.20149773792560666
1107	1	0.008837047566373712
1107	2	0.03578738190787334
1107	3	-0.002860241529482721
1107	4	-0.003527743884980339
1107	5	-0.011117376948980962
1107	6	-0.008901043238509487
1107	7	0.0033300193065954886
1107	8	0.018520720309474825
1107	9	0.027483616060344218
1107	10-11	0.014140140024018422
1107	12-13	0.0030013648837368123
1107	14-15	0.05378927214392348
1107	16-17	0.03937554058575188
1107	18-19	0.00925273809826166
1107	20-21	0.044894857466182714
1107	22-23	0.050683472487349945
1107	24-25	-8.750096041794109E-4
1107	26-27	0.04344660768057196
1107	28-29	0.03555897730809221
1107	30-31	0.07597449847852289
1107	32-33	0.06416650405224544
1107	34-35	0.09609636114124953
1107	36-37	0.07067391151672098
1107	38-39	0.018154271226805463
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2216	30-31	-0.3318732981912831
2216	32-33	-0.41426046747518797
2216	34-35	-0.3870091338884478
2216	36-37	-0.34537547644745814
2216	38-39	-0.36591596440997876
2216	40-41	-0.27321338104872694
2216	42-43	-0.35251728777322455
2216	44-45	-0.3482963143416953
2216	46-47	-0.34034916695622996
2216	48-49	-0.34524532672704566
2216	50-51	-0.30925360654860157
2216	52-53	-0.26175099732807183
2216	54-55	-0.29733651306404596
2216	56-57	-0.31376734026556363
2216	58-59	-0.3088649917878712
2216	60-61	-0.40794767909834917
2216	62-63	-0.3790240738097097
2216	64-65	-0.40764153436496287
2216	66-67	-0.4025994019569197
2216	68-69	-0.4043866058297354
2216	70-71	-0.5185092623828922
2216	72-73	-0.4030493747126087
2216	74-75	-0.4948611574483195
2216	76-77	-0.4521412863779659
2216	78-79	-0.4300322912636716
2216	80-81	-0.4801813681212934
2216	82-83	-0.4408442785622029
2216	84-85	-0.4716515096314424
2216	86-87	-0.3945395740613904
2216	88-89	-0.42482167632794443
2216	90-91	-0.3785519224867002
2216	92-93	-0.4139083690499916
2216	94-95	-0.3837528313891667
2216	96-97	-0.3374744953364015
2216	98-99	-0.33525239640701443
2216	100	-0.31152048679857813
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10.0
3	2.0
4	9.0
5	27.0
6	59.0
7	145.0
8	327.0
9	628.0
10	1111.0
11	1767.0
12	2815.0
13	4366.0
14	5317.0
15	7373.0
16	9103.0
17	13260.0
18	16080.0
19	9165.0
20	6149.0
21	5739.0
22	5693.0
23	6014.0
24	6970.0
25	8177.0
26	10293.0
27	13128.0
28	17127.0
29	21856.0
30	27729.0
31	35253.0
32	44833.0
33	58467.0
34	82064.0
35	127341.0
36	240215.0
37	551135.0
38	1018793.0
39	532833.0
40	2047.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.81635968821639	19.30876192405035	13.082282989282223	23.792595398451034
2	15.886920538785008	19.653563162218553	40.32039825824229	24.13911804075414
3	18.844825232467517	24.666492708443137	29.772466099747184	26.71621595934216
4	12.277484802723153	16.18420547803632	38.41445057355095	33.12385914568958
5	12.564314274269556	37.858318682218794	35.7707811097567	13.806585933754958
6	31.57098345669241	37.83511301050871	17.1118595797554	13.482043953043481
7	27.914381725865166	32.4362575391382	21.652581166592533	17.996779568404104
8	25.61533578966968	36.38079627659464	19.55019696144318	18.4536709722925
9	25.233360291423875	16.168667786464276	20.880043409438095	37.71792851267376
10-11	24.28672643446164	26.59503286767908	28.3588452419628	20.75939545589648
12-13	25.089107446316934	23.77011332967215	28.96841347099295	22.17236575301797
14-15	21.95975655760467	25.75376747653422	26.396900134114855	25.889575831746253
16-17	21.162275093142373	29.484122595406127	26.968518224108497	22.385084087343003
18-19	21.00397212016104	27.83375158160749	29.87609459680371	21.286181701427758
20-21	22.027496872213508	27.06798529076318	29.734449198526313	21.170068638497003
22-23	21.619051947430297	26.837970557633355	29.88573538781416	21.657242107122187
24-25	21.525324526194954	27.012205838058584	29.77233959716397	21.6901300385825
26-27	21.30202690710673	27.016274601870805	29.868118920224845	21.81357957079762
28-29	21.287895873351633	27.10659363353648	29.955023965616018	21.650486527495868
30-31	21.52966606930739	27.463865063745878	29.54804346544888	21.458425401497852
32-33	21.280574644304025	27.162970286077737	29.952193631348255	21.604261438269983
34-35	21.414633395911466	27.047304885260075	29.902834787680018	21.63522693114844
36-37	21.702962074534344	27.347371376235984	29.437346555425364	21.512319993804304
38-39	21.534163754223446	27.543385837988403	29.44108405124425	21.481366356543905
40-41	21.371140293137763	27.28016353553791	29.650965463785532	21.697730707538792
42-43	21.45879321600111	27.233873303676038	29.43273283789217	21.87460064243069
44-45	21.543755463410523	27.403610863487437	29.29829092668611	21.754342746415926
46-47	21.70144115153717	27.626079133579	28.965969446895386	21.706510267988442
48-49	21.71894184309852	27.596411037048917	28.749988971116778	21.934658148735778
50-51	21.743024588568584	27.696875067580716	28.496563655630286	22.063536688220413
52-53	21.750132990800928	27.695751646707233	28.59785744251121	21.956257919980622
54-55	21.802042984578225	27.992795246703633	28.43796263472797	21.767199133990175
56-57	21.63436056898587	27.754840379231656	28.493178978133706	22.11762007364877
58-59	21.519569290705423	27.383377384510304	28.903493243055085	22.19356008172919
60-61	21.761359862511	27.74720275752896	28.538740043947776	21.952697336012267
62-63	21.517872694380827	27.673259046613207	28.626251476051173	22.182616782954796
64-65	21.622716370246977	27.451320582563195	28.739242833740004	22.186720213449828
66-67	21.49425990497747	27.51438695881931	28.599642844676143	22.391710291527083
68-69	21.807485751276086	27.609362908975786	28.549237804298517	22.03391353544961
70-71	21.76813252137609	27.50231559884151	28.490765253575358	22.238786626207048
72-73	21.643349392760125	27.62365989037195	28.542727982802358	22.19026273406557
74-75	21.682075191296114	27.715644462262652	28.383591044507885	22.218689301933352
76-77	21.680732504163764	27.652253373779168	28.28296094202806	22.38405318002901
78-79	21.74796983292606	27.776269566165567	28.359057509635228	22.116703091273145
80-81	21.658492920118476	27.6334152436053	28.399829267196836	22.30826256907939
82-83	21.528083068594352	27.660160170538905	28.510971115110994	22.300785645755745
84-85	21.577043775186457	27.57639748118144	28.329174471732415	22.51738427189969
86-87	21.67237966077326	27.689116348771204	28.26271409039318	22.375789900062347
88-89	21.38464529340355	27.66017634886142	28.335417089682164	22.619761268052862
90-91	21.389682645011987	27.560917614335445	28.379413813983195	22.669985926669376
92-93	21.500688983963798	27.722488941176632	28.219673732309253	22.557148342550313
94-95	21.300791372065834	27.44810806000223	28.406619565095298	22.844481002836638
96-97	21.368834841296643	27.44312347649855	28.353293009614582	22.834748672590226
98-99	21.244002337718804	27.555756701673694	28.29671402586352	22.903526934743983
100	21.336446143318287	27.42436286470682	28.220272203827996	23.018918788146898
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	61.0
1	59.5
2	223.5
3	411.5
4	253.5
5	131.5
6	471.5
7	1152.0
8	1945.5
9	2288.5
10	1779.5
11	1251.5
12	1288.0
13	1510.5
14	1673.0
15	1801.5
16	2070.0
17	2368.0
18	2668.5
19	3222.5
20	3892.5
21	4743.5
22	6146.5
23	8405.5
24	11235.0
25	14493.0
26	19053.5
27	25119.0
28	32542.0
29	40696.0
30	48504.0
31	55951.0
32	62700.0
33	69459.5
34	76576.0
35	81956.0
36	86430.0
37	91735.0
38	96003.0
39	98418.5
40	100671.5
41	102083.5
42	101413.0
43	100896.0
44	100331.0
45	99281.5
46	98279.5
47	96149.0
48	93775.0
49	90966.0
50	88191.0
51	85434.5
52	80501.5
53	76546.5
54	73919.0
55	70342.5
56	65285.0
57	59163.0
58	53876.5
59	49396.5
60	44352.0
61	38092.5
62	32603.0
63	27978.0
64	23302.5
65	19041.0
66	15333.5
67	11844.5
68	8963.0
69	6950.5
70	5091.0
71	3576.5
72	2645.5
73	2015.5
74	1548.5
75	1073.5
76	693.5
77	426.0
78	286.5
79	200.0
80	124.0
81	58.5
82	26.0
83	11.0
84	6.5
85	6.5
86	3.5
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0368353021683683E-4
2	7.257847115178578E-4
3	1.7280588369472804E-4
4	0.010472036551900519
5	3.8017294412840167E-4
6	0.003628923557589289
7	6.566623580399665E-4
8	0.13900505284403922
9	0.0013824470695578243
10-11	0.0
12-13	1.0368353021683683E-4
14-15	1.5552529532525522E-4
16-17	0.0
18-19	1.0368353021683683E-4
20-21	0.0
22-23	1.5552529532525522E-4
24-25	3.2833117901998326E-4
26-27	0.00585811945725128
28-29	0.007344250057025941
30-31	0.014152801874598225
32-33	0.017280588369472803
34-35	0.026594825500618646
36-37	0.037637121468711766
38-39	0.04257936974238099
40-41	0.05431288924525302
42-43	0.07110962114038058
44-45	0.1095416496740881
46-47	0.1164711656102467
48-49	0.11341250146885001
50-51	0.11603915090100989
52-53	0.10999094497169441
54-55	0.11693774149622246
56-57	0.10174810431945586
58-59	0.09170808247679217
60-61	0.09386815602297627
62-63	0.09274491777896055
64-65	0.0
66-67	3.456117673894561E-5
68-69	1.0368353021683683E-4
70-71	0.0
72-73	0.0
74-75	0.0
76-77	1.0368353021683683E-4
78-79	1.7280588369472806E-5
80-81	3.456117673894561E-4
82-83	1.3824470695578245E-4
84-85	0.0
86-87	5.529788278231298E-4
88-89	2.592088255420921E-4
90-91	1.3824470695578245E-4
92-93	0.0010022741254294225
94-95	9.504323603210042E-4
96-97	1.0368353021683683E-4
98-99	3.456117673894561E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2893420.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.08454760883151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.36910357034114	46.03887595277456
2	14.630354708221963	17.873771974169532
3	4.557542499949143	8.351862654522492
4	2.0239409448226424	4.945260680059285
5	1.0438500545675686	3.1881554177357003
6	0.5876410333418739	2.153747200684479
7	0.36486374917343395	1.5601275939984969
8	0.26417541062830413	1.2909628358085807
9	0.18356075659190552	1.0091453197636249
>10	0.9181191973904913	9.812971147446605
>50	0.043330403069155796	1.799383473086268
>100	0.012951607702914092	1.2701070847507656
>500	2.8303209999508693E-4	0.09747474933110907
>1k	1.6981925973839706E-4	0.19446540953172814
>5k	1.1321283982559802E-4	0.4136885063366991
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6515	0.22516606645423065	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5449	0.1883238520505146	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2941	0.10164442078923903	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	3.4561176738945605E-5	0.0
22-23	0.0	0.0	0.0	6.912235347789121E-5	0.0
24-25	0.0	0.0	0.0	1.0368353021683683E-4	0.0
26-27	0.0	0.0	0.0	3.8017294412840167E-4	0.0
28-29	0.0	0.0	0.0	0.001226921774232569	0.0
30-31	0.0	0.0	0.0	0.004907687096930276	0.0
32-33	0.0	0.0	0.0	0.013357894809602477	0.0
34-35	0.0	0.0	0.0	0.02490132784041031	0.0
36-37	0.0	0.0	0.0	0.04632925741855659	0.0
38-39	0.0	0.0	0.0	0.08807915891920289	0.0
40-41	0.0	0.0	0.0	0.13762260577448143	0.0
42-43	0.0	0.0	0.0	0.18796095969475568	0.0
44-45	0.0	0.0	0.0	0.24471041190010437	0.0
46-47	0.0	0.0	0.0	0.3156645077451597	0.0
48-49	0.0	0.0	0.0	0.39819659779776184	0.0
50-51	0.0	0.0	0.0	0.4861893537751173	0.0
52-53	0.0	0.0	0.0	0.5771025291869137	0.0
54-55	0.0	0.0	0.0	0.6776064311437676	0.0
56-57	0.0	0.0	0.0	0.7916237532055492	0.0
58-59	0.0	0.0	0.0	0.9262222560153728	0.0
60-61	0.0	0.0	0.0	1.0744033012836023	0.0
62-63	0.0	0.0	0.0	1.2253665212793163	0.0
64-65	0.0	0.0	0.0	1.3821360189671736	0.0
66-67	0.0	0.0	0.0	1.5613184397702373	0.0
68-69	0.0	0.0	0.0	1.7666982325414216	0.0
70-71	0.0	0.0	0.0	1.994266300779009	0.0
72-73	0.0	0.0	0.0	2.225584256692772	0.0
74-75	0.0	0.0	0.0	2.460600258517602	0.0
76-77	0.0	0.0	0.0	2.711116948109849	0.0
78-79	0.0	0.0	0.0	2.999063392110375	0.0
80-81	0.0	0.0	0.0	3.319030766359533	0.0
82-83	0.0	0.0	0.0	3.645409238893766	0.0
84-85	0.0	0.0	0.0	3.964132410780322	0.0
86-87	0.0	0.0	0.0	4.300274415743307	3.4561176738945605E-5
88	0.0	0.0	0.0	4.577524175543129	3.4561176738945605E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4455	0.0	50.832138	1
GTATCAA	7890	0.0	42.635864	1
TCAACGC	9240	0.0	35.847862	4
ATCAACG	9350	0.0	35.3758	3
CAACGCA	9470	0.0	34.973583	5
AACGCAG	9850	0.0	33.67211	6
TATCAAC	10005	0.0	33.247375	2
TACACCG	465	0.0	29.340162	5
ACGCAGA	11550	0.0	28.509422	7
CGCAGAG	11775	0.0	27.924706	8
GTACATG	8925	0.0	26.478832	1
TACATGG	8995	0.0	25.891613	2
GTGGTAT	2085	0.0	25.688448	1
GCAGAGT	12925	0.0	25.186436	9
ACATGGG	9110	0.0	24.73886	3
TGGTATC	2120	0.0	22.637026	2
GAGTACT	8835	0.0	21.984951	12-13
GAGTACA	6375	0.0	20.930384	1
CAGAGTA	12895	0.0	20.80038	10-11
CCGCGCA	185	7.2198127E-6	20.313383	9
>>END_MODULE