FastQCFastQC Report
Wed 25 May 2016
SRR1032993_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032993_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6454314
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93000.033.052751
TCAACGC91500.031.5398854
ATCAACG95050.030.6091563
GTACATG204700.030.2169651
CAACGCA96350.030.0493495
AACGCAG98150.029.4978086
GGTATCA61300.029.44311
TACATGG206750.028.2123362
TATCAAC109250.027.3190172
ACATGGG211350.026.6865123
ACGCAGA117100.024.4432957
CATGGGG142600.023.1712044
CGCAGAG124600.022.5946048
GAGTACA144800.019.248591
GCAGAGT148600.018.312849
AGTACAT145650.017.8454482
GTATATA41900.017.1628821
TACACCG11650.016.5410845
GTCGTAC6000.016.4505651
ACACCGT12350.016.3644286
Produced by FastQC (version 0.11.5)