##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032993_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6454314 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06104583693945 34.0 31.0 34.0 30.0 34.0 2 32.14698541161772 34.0 31.0 34.0 30.0 34.0 3 32.21986472923381 34.0 31.0 34.0 30.0 34.0 4 35.558428052927084 37.0 37.0 37.0 35.0 37.0 5 35.618515461131885 37.0 37.0 37.0 35.0 37.0 6 35.6945100594734 37.0 37.0 37.0 35.0 37.0 7 35.629418401397885 37.0 37.0 37.0 35.0 37.0 8 35.629171589730525 37.0 37.0 37.0 35.0 37.0 9 37.34889579279843 39.0 39.0 39.0 35.0 39.0 10-11 37.33859376844696 39.0 39.0 39.0 35.0 39.0 12-13 37.29894183952005 39.0 39.0 39.0 35.0 39.0 14-15 38.83620962661563 41.0 40.0 41.0 36.0 41.0 16-17 38.86137953622956 41.0 40.0 41.0 36.0 41.0 18-19 38.8787100379684 41.0 40.0 41.0 36.0 41.0 20-21 38.916473075217596 41.0 40.0 41.0 36.0 41.0 22-23 38.90695386062717 41.0 40.0 41.0 36.0 41.0 24-25 38.93526337578246 41.0 40.0 41.0 36.0 41.0 26-27 38.90690598567098 41.0 40.0 41.0 36.0 41.0 28-29 38.8863556994593 41.0 40.0 41.0 35.0 41.0 30-31 38.84196895285851 41.0 39.0 41.0 35.0 41.0 32-33 38.79317228755837 41.0 39.0 41.0 35.0 41.0 34-35 38.727112749705086 41.0 39.0 41.0 35.0 41.0 36-37 38.64619981302428 41.0 39.0 41.0 35.0 41.0 38-39 38.56235279225646 41.0 39.0 41.0 35.0 41.0 40-41 38.48577850411368 41.0 39.0 41.0 34.5 41.0 42-43 38.38547969621558 41.0 39.0 41.0 34.0 41.0 44-45 38.27115786433694 40.5 38.0 41.0 34.0 41.0 46-47 38.16066897272119 40.0 38.0 41.0 34.0 41.0 48-49 38.0360912871608 40.0 38.0 41.0 33.0 41.0 50-51 37.905590431454065 40.0 38.0 41.0 33.0 41.0 52-53 37.75692893156422 40.0 37.5 41.0 33.0 41.0 54-55 37.594238132820934 40.0 37.0 41.0 33.0 41.0 56-57 37.41252067996692 40.0 37.0 41.0 32.5 41.0 58-59 37.20592227152258 40.0 36.0 41.0 32.0 41.0 60-61 36.962962833850355 39.5 36.0 41.0 32.0 41.0 62-63 36.71080179861097 39.0 35.0 41.0 31.0 41.0 64-65 36.43301991505217 39.0 35.0 41.0 31.0 41.0 66-67 36.13320943170723 38.0 35.0 40.0 31.0 41.0 68-69 35.81992578916985 37.5 35.0 40.0 31.0 41.0 70-71 35.477458797325326 37.0 35.0 40.0 30.5 41.0 72-73 35.17544443917665 36.5 35.0 39.0 30.0 41.0 74-75 34.88390175935041 36.0 35.0 39.0 30.0 41.0 76-77 34.55004551994217 35.5 34.5 38.5 30.0 40.0 78-79 34.17886997750652 35.0 34.0 37.0 30.0 39.0 80-81 33.85464915403868 35.0 34.0 37.0 29.5 39.0 82-83 33.53529670232963 35.0 34.0 36.5 29.0 39.0 84-85 33.3611736274374 35.0 34.0 36.0 29.0 37.0 86-87 33.13003976875002 35.0 34.0 36.0 29.0 37.0 88-89 32.899351348570896 35.0 34.0 35.5 29.0 37.0 90-91 32.705092903753986 35.0 34.0 35.0 29.0 36.0 92-93 32.52145441018209 35.0 34.0 35.0 29.0 36.0 94-95 32.347979506420046 35.0 34.0 35.0 28.5 36.0 96-97 32.183074064881254 35.0 34.0 35.0 28.0 36.0 98-99 32.017571116000866 35.0 34.0 35.0 27.0 35.0 100 31.896959924788288 35.0 34.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2928175533844666 1101 2 0.37707172346109985 1101 3 0.31311815359615736 1101 4 0.25103447383515487 1101 5 0.2507782883624472 1101 6 0.2527523824624254 1101 7 0.27970451603231794 1101 8 0.24994197220504333 1101 9 0.2915570108950476 1101 10-11 0.3188862553830063 1101 12-13 0.1519476457384883 1101 14-15 0.22306879840657245 1101 16-17 0.2242507157224196 1101 18-19 0.2286298741612498 1101 20-21 0.22477843642226958 1101 22-23 0.25756217378569346 1101 24-25 0.23787720801178125 1101 26-27 0.30101866758655405 1101 28-29 0.2841859377827518 1101 30-31 0.2621031394782207 1101 32-33 0.27797137327232235 1101 34-35 0.31080893356103445 1101 36-37 0.27686993944806915 1101 38-39 0.3000229185366621 1101 40-41 0.30051878485609507 1101 42-43 0.2734107232530576 1101 44-45 0.2600122317504727 1101 46-47 0.22832130554839836 1101 48-49 0.3035161487113385 1101 50-51 0.27806363348071983 1101 52-53 0.24142574404147155 1101 54-55 0.2785019374548554 1101 56-57 0.30085620439361804 1101 58-59 0.2838028656100988 1101 60-61 0.31894977135251423 1101 62-63 0.3088421817260496 1101 64-65 0.30242121321314386 1101 66-67 0.3065894821140347 1101 68-69 0.30282315693833084 1101 70-71 0.3107895569822148 1101 72-73 0.27661191673724517 1101 74-75 0.3168824293492776 1101 76-77 0.2933958251760629 1101 78-79 0.34654478104281594 1101 80-81 0.29839134994949745 1101 82-83 0.26053944875280166 1101 84-85 0.2311105884821174 1101 86-87 0.18749140888307636 1101 88-89 0.2080023116097678 1101 90-91 0.25293729409541754 1101 92-93 0.2300847108172448 1101 94-95 0.1995824805644233 1101 96-97 0.1991120383493623 1101 98-99 0.23629554533869168 1101 100 0.19050369688562085 1102 1 0.25097883192312054 1102 2 0.23663339368165737 1102 3 0.26587537214021495 1102 4 0.22598911870831273 1102 5 0.24717516434665754 1102 6 0.17076433346655762 1102 7 0.1935918335730804 1102 8 0.20578657567237002 1102 9 0.1955388879518054 1102 10-11 0.16509252728535273 1102 12-13 0.21535034055417412 1102 14-15 0.2690904148643014 1102 16-17 0.2621125806080826 1102 18-19 0.2385699980175673 1102 20-21 0.22473461438622877 1102 22-23 0.23530138923025135 1102 24-25 0.2574966601726416 1102 26-27 0.26653019445597437 1102 28-29 0.287344438249292 1102 30-31 0.2980212316834425 1102 32-33 0.2892871756318982 1102 34-35 0.32786882497438796 1102 36-37 0.3130859466470781 1102 38-39 0.34580911403460135 1102 40-41 0.3401752233597506 1102 42-43 0.3546019120733064 1102 44-45 0.33373240306359975 1102 46-47 0.330227415774651 1102 48-49 0.3622504901928352 1102 50-51 0.3753858022294736 1102 52-53 0.3262540938067531 1102 54-55 0.3306246292481845 1102 56-57 0.35269227693536465 1102 58-59 0.3174427154861448 1102 60-61 0.41154844857482686 1102 62-63 0.38596766547301087 1102 64-65 0.3420103740040048 1102 66-67 0.3141247152351525 1102 68-69 0.35520226888449713 1102 70-71 0.3492334928249008 1102 72-73 0.3101875373899645 1102 74-75 0.30437932880413854 1102 76-77 0.22859718076310998 1102 78-79 0.23252396007752196 1102 80-81 0.21976462803948493 1102 82-83 0.22920685629313198 1102 84-85 0.1917598086798762 1102 86-87 0.19334274624587522 1102 88-89 0.16180437269893133 1102 90-91 0.13919323242360804 1102 92-93 0.12996383140532686 1102 94-95 0.10754043846516481 1102 96-97 0.0892774874937956 1102 98-99 0.10360065098423377 1102 100 0.06566884267812867 1103 1 0.25391438804572886 1103 2 0.27158886176787433 1103 3 0.31266613409157173 1103 4 0.2922745921546621 1103 5 0.2446684662629508 1103 6 0.20247203416683135 1103 7 0.2398290357507591 1103 8 0.24138047434234267 1103 9 0.28580739538249134 1103 10-11 0.32923536983267354 1103 12-13 0.2843834363058164 1103 14-15 0.352378680026348 1103 16-17 0.38403808949416174 1103 18-19 0.345070442789698 1103 20-21 0.33583479988005394 1103 22-23 0.32141578970355056 1103 24-25 0.35077319224872383 1103 26-27 0.40477961343493263 1103 28-29 0.40989417383644877 1103 30-31 0.4202088531197816 1103 32-33 0.4031120130894763 1103 34-35 0.3696665043993832 1103 36-37 0.3791081064795563 1103 38-39 0.37409210587465935 1103 40-41 0.423822332385285 1103 42-43 0.42087854073057684 1103 44-45 0.45416620400146 1103 46-47 0.4722521628025831 1103 48-49 0.467648097470466 1103 50-51 0.4416330896860572 1103 52-53 0.4380752503699128 1103 54-55 0.4577236347753555 1103 56-57 0.4286337469646426 1103 58-59 0.45779099312306926 1103 60-61 0.41141954964493266 1103 62-63 0.420554191622891 1103 64-65 0.41842943653364273 1103 66-67 0.3565008308111004 1103 68-69 0.3791298254795876 1103 70-71 0.3933544013396997 1103 72-73 0.35758381164364295 1103 74-75 0.34539294215518623 1103 76-77 0.352356557120757 1103 78-79 0.36364201818117436 1103 80-81 0.37129019945398767 1103 82-83 0.33821648613395183 1103 84-85 0.32339424580545995 1103 86-87 0.3209118833184874 1103 88-89 0.3537549514379634 1103 90-91 0.27211689383887006 1103 92-93 0.2880914210192316 1103 94-95 0.2732910743696024 1103 96-97 0.2799270467152013 1103 98-99 0.22852116211408458 1103 100 0.22389497825570714 1104 1 0.07896287383887568 1104 2 0.11794096374364216 1104 3 0.09950373824304393 1104 4 0.0541086457407971 1104 5 0.08089456506860415 1104 6 0.05693745499741709 1104 7 0.08064863099652086 1104 8 0.08942708565115254 1104 9 0.13988953196840015 1104 10-11 0.15196593973583106 1104 12-13 0.14664436210448883 1104 14-15 0.18335073902578358 1104 16-17 0.15848451321259205 1104 18-19 0.2231337626120009 1104 20-21 0.21746153506555999 1104 22-23 0.21406747542083338 1104 24-25 0.2274462568643827 1104 26-27 0.24766255843656637 1104 28-29 0.21596147303127822 1104 30-31 0.2170167784547843 1104 32-33 0.2638049499634718 1104 34-35 0.2531422044248117 1104 36-37 0.23986006379796976 1104 38-39 0.2728213969517128 1104 40-41 0.26585256893250175 1104 42-43 0.1930378661961285 1104 44-45 0.17819299983109005 1104 46-47 0.15871685984827621 1104 48-49 0.2261037722575594 1104 50-51 0.21426394990256625 1104 52-53 0.19909926703390113 1104 54-55 0.24440471023343946 1104 56-57 0.23780587640508344 1104 58-59 0.23218141590295005 1104 60-61 0.24937649769227477 1104 62-63 0.2482393099316198 1104 64-65 0.2730858769080413 1104 66-67 0.2621615501179093 1104 68-69 0.2683988926380607 1104 70-71 0.28368678904266886 1104 72-73 0.2522479139674658 1104 74-75 0.26943617032246436 1104 76-77 0.25692592739776643 1104 78-79 0.24159817356153468 1104 80-81 0.17647659983867214 1104 82-83 0.2255027963269285 1104 84-85 0.2073704678380821 1104 86-87 0.18705797928916468 1104 88-89 0.20914041881651002 1104 90-91 0.22152382179808683 1104 92-93 0.14576384968337663 1104 94-95 0.1481160084005566 1104 96-97 0.1508383151549495 1104 98-99 0.20691649808448176 1104 100 0.14776588913814948 1105 1 0.02881551877327837 1105 2 0.04432282660735609 1105 3 0.05442386494595297 1105 4 0.0894125854688852 1105 5 0.0340599577762859 1105 6 0.02435076688845328 1105 7 0.03319060430278853 1105 8 0.0723593298211469 1105 9 0.10952958180246242 1105 10-11 0.0638374859821198 1105 12-13 0.10236989115659867 1105 14-15 0.1567312112506727 1105 16-17 0.16592612912245386 1105 18-19 0.1410144363285042 1105 20-21 0.1272829438051346 1105 22-23 0.16855857600222635 1105 24-25 0.14535849701277215 1105 26-27 0.16893161126380107 1105 28-29 0.22705616891879288 1105 30-31 0.1823065602973699 1105 32-33 0.2136935213990938 1105 34-35 0.23888316765227557 1105 36-37 0.24878080091058052 1105 38-39 0.16960381268631863 1105 40-41 0.18650434876333577 1105 42-43 0.1747115722397794 1105 44-45 0.16460127108240385 1105 46-47 0.19293745749743607 1105 48-49 0.12908309224891923 1105 50-51 0.16765024887239832 1105 52-53 0.18863903762374434 1105 54-55 0.1879094702891848 1105 56-57 0.1831644944581967 1105 58-59 0.1661614164647034 1105 60-61 0.23723818806647756 1105 62-63 0.21212458534285616 1105 64-65 0.22911411934529013 1105 66-67 0.22132325451249102 1105 68-69 0.21815433505641124 1105 70-71 0.23176734784607333 1105 72-73 0.20929257553265757 1105 74-75 0.24127850175378285 1105 76-77 0.21118854154255473 1105 78-79 0.25486165725222776 1105 80-81 0.24267790647270715 1105 82-83 0.23086642787215794 1105 84-85 0.23996921958901396 1105 86-87 0.23433039747881423 1105 88-89 0.2487155224210369 1105 90-91 0.23321084074346743 1105 92-93 0.22547389097005777 1105 94-95 0.23720219075323 1105 96-97 0.2401996989727948 1105 98-99 0.18390426955902228 1105 100 0.1758904634878462 1106 1 0.03720203283472756 1106 2 0.07488323863943691 1106 3 0.0692841174344423 1106 4 0.08037686393642929 1106 5 0.06102661514412944 1106 6 0.08270648618740495 1106 7 0.04230280728958746 1106 8 0.0710290924110808 1106 9 0.06098217217768109 1106 10-11 0.08083146239552264 1106 12-13 0.12228761982765946 1106 14-15 0.16452142087403843 1106 16-17 0.1168475049440616 1106 18-19 0.08200760536298901 1106 20-21 0.12657368136671465 1106 22-23 0.15468469120975925 1106 24-25 0.1591447160023165 1106 26-27 0.19341750506715272 1106 28-29 0.1148558311929122 1106 30-31 0.10482176851918723 1106 32-33 0.12829408081815075 1106 34-35 0.11702888890525998 1106 36-37 0.1466483259139295 1106 38-39 0.11147268205173333 1106 40-41 0.10080188906353271 1106 42-43 0.12445805380445307 1106 44-45 0.09837045424244195 1106 46-47 0.08804149070122946 1106 48-49 0.12278502146224213 1106 50-51 0.12553531263690587 1106 52-53 0.11432747012300126 1106 54-55 0.14172447847526826 1106 56-57 0.07063302832675333 1106 58-59 0.05620039590773729 1106 60-61 0.1334079547168372 1106 62-63 0.07696731083139952 1106 64-65 0.1229531394372998 1106 66-67 0.1443313021469521 1106 68-69 0.09243796688826222 1106 70-71 0.10164161325363352 1106 72-73 0.09762516613638184 1106 74-75 0.048023862475346846 1106 76-77 0.08466831162103006 1106 78-79 0.0863865275303084 1106 80-81 0.05763904772206985 1106 82-83 0.02906837441926058 1106 84-85 0.03104613122353328 1106 86-87 0.049758145046780555 1106 88-89 0.0538699389589965 1106 90-91 0.03138846959323871 1106 92-93 0.034513846537329584 1106 94-95 0.022556714721424953 1106 96-97 0.005790986135615128 1106 98-99 -0.013148063727392412 1106 100 0.01382776269672803 1107 1 0.08019481674686091 1107 2 0.11809965043374149 1107 3 0.0999592161782914 1107 4 0.12234533652312507 1107 5 0.10889373172780381 1107 6 0.09510977397400211 1107 7 0.0854073463130689 1107 8 0.10721837745978746 1107 9 0.09247866243289593 1107 10-11 0.07534929551311365 1107 12-13 0.06374894904539019 1107 14-15 0.12140338309954757 1107 16-17 0.11137750512129685 1107 18-19 0.1018184557724382 1107 20-21 0.11318493662316342 1107 22-23 0.10985305545132462 1107 24-25 0.08373185237075376 1107 26-27 0.14131128863667186 1107 28-29 0.11514098428570207 1107 30-31 0.05104959014882837 1107 32-33 0.07443446006310239 1107 34-35 0.0933059785159287 1107 36-37 0.09063879909017913 1107 38-39 0.03269752235064516 1107 40-41 0.061348553609335 1107 42-43 0.04627408484627438 1107 44-45 0.07858870316113808 1107 46-47 0.03941180699696645 1107 48-49 0.07289299061248755 1107 50-51 0.026425257908236688 1107 52-53 0.0187032164785208 1107 54-55 0.022619714358505405 1107 56-57 0.018937769711705243 1107 58-59 0.03150406726530264 1107 60-61 0.09081907439961157 1107 62-63 0.025744230397378942 1107 64-65 0.07758033960669763 1107 66-67 0.04139030295207391 1107 68-69 0.031845036496498835 1107 70-71 0.12158853150108229 1107 72-73 0.09909235843286268 1107 74-75 0.033430097492818334 1107 76-77 0.02436021343256556 1107 78-79 0.028520991969124054 1107 80-81 0.030297327556972675 1107 82-83 -0.036646240955491294 1107 84-85 -0.020998650915025507 1107 86-87 -0.04409624799070855 1107 88-89 -0.08221769257935563 1107 90-91 -0.07196956827280587 1107 92-93 -0.020295997509201413 1107 94-95 -0.07039768492531806 1107 96-97 -0.09689022628026578 1107 98-99 -0.05346381106547682 1107 100 -0.0385318922490967 1108 1 0.02935383022037996 1108 2 0.03400001683283449 1108 3 0.013340537101100836 1108 4 0.01617518843003296 1108 5 0.03448878142393852 1108 6 0.01896643255731334 1108 7 0.011065636798953449 1108 8 -0.042954393332770735 1108 9 -0.014928630416498834 1108 10-11 0.022269953908491402 1108 12-13 0.04604189066309061 1108 14-15 0.024572808342952612 1108 16-17 0.06776400895842016 1108 18-19 0.059031294688885794 1108 20-21 0.08972281820876304 1108 22-23 0.11189882704837828 1108 24-25 0.0816857078073383 1108 26-27 0.11267439476103647 1108 28-29 0.043495111413463405 1108 30-31 0.05572658324251023 1108 32-33 0.08677741401780281 1108 34-35 0.08076837699231021 1108 36-37 0.10565336730962827 1108 38-39 0.12086232582552014 1108 40-41 0.07118721214108348 1108 42-43 0.06698056938150643 1108 44-45 0.05499292484200424 1108 46-47 0.09977838511120041 1108 48-49 0.10594386987659732 1108 50-51 0.06960521388247543 1108 52-53 0.1158474406197385 1108 54-55 0.11349733721763755 1108 56-57 0.13247163796463468 1108 58-59 0.1488782986391044 1108 60-61 0.15614971219774532 1108 62-63 0.07212109055051741 1108 64-65 0.1343752692015272 1108 66-67 0.1307228334574262 1108 68-69 0.1419581083513961 1108 70-71 0.14799379391137535 1108 72-73 0.11304784012540381 1108 74-75 0.12205012429673445 1108 76-77 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74-75 -0.3471764553052523 2214 76-77 -0.33104523043556355 2214 78-79 -0.3123203124927798 2214 80-81 -0.32662486994362183 2214 82-83 -0.3279961158646998 2214 84-85 -0.32042321255588746 2214 86-87 -0.3090282189803446 2214 88-89 -0.29457669531140596 2214 90-91 -0.28215457771445074 2214 92-93 -0.31862811524910484 2214 94-95 -0.32958853870285765 2214 96-97 -0.4033837937658191 2214 98-99 -0.40099605189594456 2214 100 -0.3533758593492635 2215 1 -0.2270179205939229 2215 2 -0.26286139961580446 2215 3 -0.25114770628372796 2215 4 -0.24437135629026585 2215 5 -0.24114731614415774 2215 6 -0.1911270824925424 2215 7 -0.27188246112021375 2215 8 -0.280266865372532 2215 9 -0.3098681409799866 2215 10-11 -0.30105190036155705 2215 12-13 -0.27678849496770397 2215 14-15 -0.3412227446863412 2215 16-17 -0.37008363058862415 2215 18-19 -0.31737334309190857 2215 20-21 -0.3504493222908778 2215 22-23 -0.3406279400083747 2215 24-25 -0.3474108425448037 2215 26-27 -0.323965127648286 2215 28-29 -0.33914898816354366 2215 30-31 -0.30977117693411316 2215 32-33 -0.37888278512333784 2215 34-35 -0.38215741503913847 2215 36-37 -0.35824450601099755 2215 38-39 -0.326641314101316 2215 40-41 -0.31125060661582893 2215 42-43 -0.3401989044900091 2215 44-45 -0.32954341921450236 2215 46-47 -0.28982119493436187 2215 48-49 -0.3016628090923277 2215 50-51 -0.27715821949578867 2215 52-53 -0.32775752457530416 2215 54-55 -0.3615375045078295 2215 56-57 -0.30976296177823315 2215 58-59 -0.297638212261063 2215 60-61 -0.31284922072361354 2215 62-63 -0.2857206991034644 2215 64-65 -0.29220357678102005 2215 66-67 -0.35327940868950236 2215 68-69 -0.2745197055214774 2215 70-71 -0.26344056817256245 2215 72-73 -0.2676153924796054 2215 74-75 -0.25739680343103544 2215 76-77 -0.2206364747298224 2215 78-79 -0.22162777701301906 2215 80-81 -0.24777666458167147 2215 82-83 -0.27283558106987016 2215 84-85 -0.23657342357552835 2215 86-87 -0.24481464334203906 2215 88-89 -0.2005783917668893 2215 90-91 -0.24808276219008008 2215 92-93 -0.24755983523727565 2215 94-95 -0.29119786199314746 2215 96-97 -0.2566433182683596 2215 98-99 -0.28092579525570827 2215 100 -0.2658149474862803 2216 1 -0.1374474497108693 2216 2 -0.17304480648374465 2216 3 -0.2137446440332127 2216 4 -0.20276869458371038 2216 5 -0.17063546091841175 2216 6 -0.19582808493217385 2216 7 -0.19258434046073347 2216 8 -0.23900962317956242 2216 9 -0.2493696918490116 2216 10-11 -0.3150768195403373 2216 12-13 -0.3339545086182767 2216 14-15 -0.3910794192074576 2216 16-17 -0.3664937934612027 2216 18-19 -0.3664796002270876 2216 20-21 -0.3560712827582222 2216 22-23 -0.35773955803897906 2216 24-25 -0.3959327400981749 2216 26-27 -0.41419520230247286 2216 28-29 -0.38877694155823406 2216 30-31 -0.3964674258305152 2216 32-33 -0.47835309688011307 2216 34-35 -0.4306688526379432 2216 36-37 -0.4237405961088925 2216 38-39 -0.41974300780415774 2216 40-41 -0.3924566808319554 2216 42-43 -0.3716058098338806 2216 44-45 -0.37435506965650944 2216 46-47 -0.32363552871623114 2216 48-49 -0.3404375232656349 2216 50-51 -0.3469798141250209 2216 52-53 -0.40886328927318516 2216 54-55 -0.3483286063931814 2216 56-57 -0.3564362913234831 2216 58-59 -0.3708342749991971 2216 60-61 -0.34005016387409626 2216 62-63 -0.3877466139542278 2216 64-65 -0.35487620218333404 2216 66-67 -0.3712868230138042 2216 68-69 -0.3988332288255805 2216 70-71 -0.44863155730249815 2216 72-73 -0.4177977813095666 2216 74-75 -0.39800245311761273 2216 76-77 -0.4073076679378147 2216 78-79 -0.3513162226868971 2216 80-81 -0.33161782790600824 2216 82-83 -0.29935277096885926 2216 84-85 -0.33236679618687504 2216 86-87 -0.3333047021433657 2216 88-89 -0.320702052531189 2216 90-91 -0.34574379102157593 2216 92-93 -0.3614462299117065 2216 94-95 -0.3526929471641651 2216 96-97 -0.28184428918351756 2216 98-99 -0.29668398192955436 2216 100 -0.36908969414810855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55107.0 3 8384.0 4 5471.0 5 5026.0 6 4316.0 7 5457.0 8 6552.0 9 6900.0 10 7031.0 11 7462.0 12 8337.0 13 9057.0 14 9861.0 15 10193.0 16 10007.0 17 10026.0 18 9953.0 19 9829.0 20 9954.0 21 10437.0 22 12498.0 23 17688.0 24 27097.0 25 37912.0 26 40946.0 27 35368.0 28 36713.0 29 47206.0 30 58573.0 31 71621.0 32 93049.0 33 127975.0 34 188715.0 35 299622.0 36 584583.0 37 1299493.0 38 2228938.0 39 1035095.0 40 1862.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.304150868736905 19.40812981506402 13.12671323518809 24.161006081010996 2 15.34973725708491 20.01846720636599 40.43865429730807 24.19314123924103 3 18.13253224085556 25.4669847839877 29.895709432172367 26.504773542984374 4 12.147471988949711 16.533660243923844 39.04667066311349 32.27219710401295 5 12.844538575435068 38.37063348781835 35.69954516620669 13.085282770539894 6 30.934610813354617 38.094153420099225 17.97162574011634 12.99961002642982 7 27.65360243899489 32.541500406096 22.50466490955332 17.30023224535579 8 25.392962249698414 36.50788777557596 20.23550134744977 17.863648627275857 9 25.468796638355773 16.795676999949606 20.634665405533223 37.1008609561614 10-11 24.03890926417771 26.782153800257113 28.92498070709187 20.253956228473307 12-13 24.65811687176463 24.190555696871133 28.823193899694644 22.328133531669597 14-15 21.95006361051911 26.021826819683362 25.734017324078735 26.294092245718794 16-17 21.892113970686566 29.41517719085377 26.291338375739333 22.40137046272033 18-19 21.883778251851723 28.547446768626507 28.006795220677215 21.561979758844554 20-21 22.88854009711661 27.849028911402723 27.512355094900958 21.750075896579705 22-23 22.663245282223127 27.578613075623977 27.48766713864592 22.270474503506975 24-25 22.41472061073271 27.680469920342617 27.49096490412685 22.41384456479782 26-27 22.17789301201711 27.706539388030528 27.55420908874401 22.56135851120836 28-29 22.263133409879014 27.773158731041008 27.644130344098176 22.3195775149818 30-31 22.398157841855483 28.06535096641668 27.31555160548467 22.22093958624317 32-33 22.18078142701239 27.848030904122655 27.636425205661435 22.334762463203518 34-35 22.347793715893594 27.593807929338375 27.676540870010673 22.381857484757354 36-37 22.54769658683837 27.879721886514258 27.263090737276713 22.309490789370663 38-39 22.331544870655396 28.052410889603525 27.448249483140202 22.167794756600877 40-41 22.23619813323003 27.745680558429335 27.656496587883616 22.361624720457016 42-43 22.188365698216575 27.723353047180193 27.589322457070363 22.498958797532868 44-45 22.216628954101587 27.802334576532576 27.609094183623107 22.371942285742726 46-47 22.29818728201621 27.875029025739188 27.523618907841392 22.303164784403208 48-49 22.200861753417694 27.826061741291465 27.517013361419057 22.45606314387179 50-51 22.199011066766715 27.789754227436035 27.542699247778685 22.46853545801856 52-53 22.261279742387323 27.70148048823718 27.576262442138024 22.460977327237476 54-55 22.269967047877497 27.988780771467336 27.43738709052142 22.303865090133748 56-57 22.168464444866913 27.776479534604892 27.490360350609393 22.564695669918805 58-59 22.08685144235395 27.433989493955956 27.78226300092519 22.6968960627649 60-61 22.331475548679123 27.811591391577 27.452951033783275 22.403982025960605 62-63 22.117901013413537 27.783313049969955 27.4696232178855 22.629162718731013 64-65 22.156267129367965 27.552908668439013 27.605171896478335 22.685652305714687 66-67 22.010714336662577 27.567096017500692 27.54841903913653 22.873770606700205 68-69 22.262265422429575 27.678370128385993 27.50478125320779 22.554583195976647 70-71 22.210231186141918 27.489067741394287 27.57889197244584 22.721809100017957 72-73 22.051391425676083 27.57977205624857 27.650136647322604 22.718699870752744 74-75 22.128129359725335 27.62421207564694 27.53162066750629 22.71603789712143 76-77 22.139848954173484 27.508531544736396 27.483226476791828 22.868393024298292 78-79 22.15147198727178 27.580107055170323 27.564306869987192 22.704114087570705 80-81 22.082246378159923 27.46807049795882 27.6723669014862 22.77731622239506 82-83 21.997330690124755 27.434000867545095 27.795981924980996 22.772686517349157 84-85 21.9841813582721 27.39589376473176 27.649836466482082 22.97008841051406 86-87 22.12038766425287 27.452186036479798 27.63561848871972 22.791807810547617 88-89 21.932760008003783 27.35400481017724 27.699700928009293 23.013534253809684 90-91 21.917248560692183 27.33680919271623 27.753569663242594 22.992372583348995 92-93 22.072617042524676 27.3602585445427 27.662492327094697 22.904632085837928 94-95 21.884017737181388 27.157122777319053 27.787920070584054 23.17093941491551 96-97 21.978088682067273 27.109156802418354 27.81085822778353 23.101896287730842 98-99 21.8778368554335 27.19313586997727 27.738215232546366 23.190812042042865 100 21.9414089735756 27.021521269244325 27.76677392132016 23.270295835859915 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5135.0 1 2954.5 2 680.0 3 564.0 4 474.0 5 386.5 6 358.0 7 357.5 8 371.0 9 378.0 10 385.0 11 388.5 12 415.5 13 456.5 14 525.0 15 615.5 16 734.0 17 922.0 18 1247.5 19 1764.5 20 2483.5 21 3562.0 22 5586.0 23 8895.5 24 13536.5 25 19922.5 26 28740.0 27 40661.0 28 56238.0 29 74312.5 30 91819.5 31 108985.5 32 126391.5 33 144415.5 34 161842.0 35 177016.0 36 191016.5 37 204244.5 38 215955.0 39 222800.0 40 229640.0 41 237593.5 42 239755.5 43 240137.5 44 238137.0 45 233758.0 46 231417.5 47 226584.0 48 222778.0 49 218368.5 50 210936.0 51 203991.0 52 191593.5 53 180583.0 54 173669.0 55 163210.0 56 149538.5 57 135877.5 58 123127.5 59 113040.5 60 102145.0 61 88579.5 62 77415.0 63 66386.0 64 54685.5 65 44072.5 66 35046.0 67 27094.0 68 20622.5 69 15811.0 70 11536.0 71 8405.0 72 6592.0 73 5059.0 74 3755.0 75 2596.5 76 1718.0 77 1281.0 78 905.0 79 580.5 80 368.5 81 235.0 82 167.5 83 112.0 84 80.0 85 70.0 86 63.0 87 46.5 88 34.0 89 29.0 90 23.0 91 16.5 92 14.0 93 18.0 94 11.5 95 5.5 96 7.5 97 6.5 98 4.5 99 3.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07580976072747622 2 0.07814928124042307 3 0.07934228176689265 4 0.08137193201322403 5 0.07971412608683122 6 0.07993103527346206 7 0.0790169179869464 8 0.07892395690696177 9 0.0788464893403079 10-11 0.07828872286040003 12-13 0.07742883287054209 14-15 0.0760576569407686 16-17 0.07457802641767972 18-19 0.07407448723442957 20-21 0.07356320129451402 22-23 0.07557735802751463 24-25 0.07561609181084156 26-27 0.07501959154760676 28-29 0.07081310267830168 30-31 0.07625907261406867 32-33 0.07465549398433358 34-35 0.0719828629347751 36-37 0.06862077054199718 38-39 0.07045675187169388 40-41 0.07319910373124085 42-43 0.07808730718709998 44-45 0.07890846339363099 46-47 0.08206139335644347 48-49 0.08498966737595971 50-51 0.0859425184458023 52-53 0.08619041465909467 54-55 0.08620590817242545 56-57 0.08409879035944022 58-59 0.08303748469628221 60-61 0.08110854228660087 62-63 0.0816275749831818 64-65 0.08101558120661623 66-67 0.08053528229336224 68-69 0.08007047689343903 70-71 0.07827322934706926 72-73 0.07820350853708077 74-75 0.07764574205717292 76-77 0.07766898232716908 78-79 0.07781617070381143 80-81 0.07910987906693105 82-83 0.0787302879903271 84-85 0.07784715773047299 86-87 0.0786063398836809 88-89 0.0753527020842184 90-91 0.07260260346800605 92-93 0.07237020076804444 94-95 0.07279627238464073 96-97 0.07178919401814042 98-99 0.07144058996819802 100 0.07057295322167467 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6454314.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.20007768570988 #Duplication Level Percentage of deduplicated Percentage of total 1 72.43259649562376 38.53419760544861 2 15.106859514553067 16.073721995226574 3 5.205901001313975 8.308630130820548 4 2.365977441236223 5.034807347056166 5 1.2893653808102272 3.4297169212184495 6 0.8054719486401164 2.571070214478859 7 0.5208403861923712 1.9396124305102496 8 0.3815996979642051 1.6240906861231312 9 0.2788284790575413 1.3350327072164574 >10 1.4782604092040927 14.181427487317134 >50 0.09559588530333304 3.461515569584242 >100 0.03826625703621067 3.303581030360761 >500 3.7882827934509014E-4 0.1189983584830915 >1k 5.827478543091049E-5 0.08359751615571634 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.5493513330773805E-5 0.0 10-11 0.0 0.0 0.0 3.873378332693451E-5 0.0 12-13 0.0 0.0 0.0 4.6480539992321415E-5 0.0 14-15 0.0 0.0 0.0 7.746756665386902E-5 0.0 16-17 0.0 0.0 0.0 9.296107998464283E-5 0.0 18-19 0.0 0.0 0.0 9.296107998464283E-5 0.0 20-21 0.0 0.0 0.0 1.0070783665002973E-4 0.0 22-23 0.0 0.0 0.0 1.2394810664619044E-4 0.0 24-25 0.0 0.0 0.0 3.098702666154761E-4 0.0 26-27 0.0 0.0 0.0 5.732599932386308E-4 0.0 28-29 0.0 1.5493513330773805E-5 0.0 0.0018824618696890172 0.0 30-31 0.0 1.5493513330773805E-5 0.0 0.0071812434288136585 0.0 32-33 0.0 1.5493513330773805E-5 0.0 0.01852249518694008 0.0 34-35 0.0 1.5493513330773805E-5 0.0 0.034016008517713886 0.0 36-37 0.0 1.5493513330773805E-5 0.0 0.06100570873992186 0.0 38-39 0.0 1.5493513330773805E-5 0.0 0.10881094412202444 0.0 40-41 0.0 1.5493513330773805E-5 0.0 0.16708979451573008 0.0 42-43 0.0 1.5493513330773805E-5 0.0 0.22849833460225208 0.0 44-45 0.0 1.5493513330773805E-5 0.0 0.3017284253601545 0.0 46-47 0.0 1.5493513330773805E-5 0.0 0.38897239892574176 0.0 48-49 0.0 1.5493513330773805E-5 0.0 0.4909739439388911 0.0 50-51 0.0 1.5493513330773805E-5 0.0 0.6000482777875387 0.0 52-53 0.0 1.5493513330773805E-5 0.0 0.7139643345520531 0.0 54-55 0.0 1.5493513330773805E-5 0.0 0.8373159409350087 0.0 56-57 0.0 1.5493513330773805E-5 0.0 0.9805457249213472 0.0 58-59 0.0 1.5493513330773805E-5 0.0 1.1437543943477184 0.0 60-61 0.0 1.5493513330773805E-5 0.0 1.3179479647256083 0.0 62-63 0.0 2.3240269996160708E-5 0.0 1.496425491539457 0.0 64-65 0.0 3.098702666154761E-5 0.0 1.6877084071211907 0.0 66-67 0.0 3.098702666154761E-5 0.0 1.903881651868812 0.0 68-69 0.0 3.098702666154761E-5 0.0 2.150646838688046 0.0 70-71 1.5493513330773805E-5 3.098702666154761E-5 0.0 2.4149739228677127 0.0 72-73 1.5493513330773805E-5 3.098702666154761E-5 0.0 2.693431091205045 0.0 74-75 1.5493513330773805E-5 3.098702666154761E-5 0.0 2.972817870342224 0.0 76-77 1.5493513330773805E-5 3.098702666154761E-5 0.0 3.2731906132859354 0.0 78-79 1.5493513330773805E-5 3.098702666154761E-5 0.0 3.6138387441329938 0.0 80-81 1.5493513330773805E-5 3.098702666154761E-5 0.0 3.9853654470482844 0.0 82-83 1.5493513330773805E-5 3.098702666154761E-5 0.0 4.367853810645098 0.0 84-85 1.5493513330773805E-5 3.098702666154761E-5 0.0 4.75024146640526 0.0 86-87 1.5493513330773805E-5 3.873378332693451E-5 0.0 5.157837068354592 0.0 88 1.5493513330773805E-5 4.6480539992321415E-5 0.0 5.48396003045405 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9300 0.0 33.05275 1 TCAACGC 9150 0.0 31.539885 4 ATCAACG 9505 0.0 30.609156 3 GTACATG 20470 0.0 30.216965 1 CAACGCA 9635 0.0 30.049349 5 AACGCAG 9815 0.0 29.497808 6 GGTATCA 6130 0.0 29.4431 1 TACATGG 20675 0.0 28.212336 2 TATCAAC 10925 0.0 27.319017 2 ACATGGG 21135 0.0 26.686512 3 ACGCAGA 11710 0.0 24.443295 7 CATGGGG 14260 0.0 23.171204 4 CGCAGAG 12460 0.0 22.594604 8 GAGTACA 14480 0.0 19.24859 1 GCAGAGT 14860 0.0 18.31284 9 AGTACAT 14565 0.0 17.845448 2 GTATATA 4190 0.0 17.162882 1 TACACCG 1165 0.0 16.541084 5 GTCGTAC 600 0.0 16.450565 1 ACACCGT 1235 0.0 16.364428 6 >>END_MODULE