FastQCFastQC Report
Wed 25 May 2016
SRR1032993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6454314
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99600.1543153927745071No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77990.1208339104667049No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99600.044.855871
GTATCAA170000.040.4014131
TCAACGC196250.034.0905954
ATCAACG197300.033.9807053
CAACGCA200650.033.3172575
AACGCAG205300.032.6316266
TATCAAC208650.032.3349382
ACGCAGA238000.027.9692157
CGCAGAG246550.026.9227528
GTACATG204750.026.7071291
TACATGG206450.025.1135432
GCAGAGT275000.023.8455379
ACATGGG214150.023.2440743
GTCGTAC5750.021.2424011
GAGTACA149850.020.3463291
GAGTACT166000.020.1335112-13
CAGAGTA271400.019.72220610-11
CATGGGG146000.019.5927494
AGAGTAC250200.019.58159310-11
AGTACTT173150.018.29835912-13
Produced by FastQC (version 0.11.5)