FastQCFastQC Report
Wed 25 May 2016
SRR1032992_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032992_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2892454
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21550.040.7853741
GTATCAA36200.034.666721
TCAACGC37350.032.2145424
ATCAACG38450.031.4151653
CAACGCA39200.030.6942125
AACGCAG41950.028.6810826
GTACATG88050.028.291541
TACATGG88700.027.1299462
TATCAAC46100.026.7117882
ACATGGG89950.025.3421333
ACGCAGA50450.023.2898337
CGCAGAG51700.022.4540128
GTACTAG4800.020.5630781
GAGTACA63800.019.5224931
CATGGGG58750.018.48024
GTACGAC2803.943387E-818.4644853
ACACCGT5100.018.4309356
GCAGAGT62450.018.0620219
GTATTAG14650.017.6455481
AGTACAT64650.017.3025862
Produced by FastQC (version 0.11.5)