FastQCFastQC Report
Wed 25 May 2016
SRR1032992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032992_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2892454
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68630.23727257200978824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55500.19187859167336802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31210.10790145668695163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43200.054.1512261
GTATCAA80500.043.823471
TCAACGC97600.035.383464
ATCAACG97900.035.2750323
CAACGCA99050.034.9087455
TATCAAC103450.033.653692
AACGCAG103450.033.4239856
TAGACCG1252.3461143E-730.1079655
ACGCAGA120800.028.3503447
CGCAGAG122450.027.9294268
GTACATG90000.026.3579461
TACATGG90800.024.818822
GCAGAGT137300.024.7677029
GTACTAG5450.024.1337181
TACACCG4700.024.0223125
ACATGGG92350.023.5374363
CGCGCAC2704.7293724E-1122.61386194
GTGGTAT20900.022.0263481
TATAACG2206.248956E-821.384982
GAGTACT90100.021.2946212-13
Produced by FastQC (version 0.11.5)