##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032992_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2892454 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.86971512770817 34.0 31.0 34.0 31.0 34.0 2 32.914158012538834 34.0 31.0 34.0 31.0 34.0 3 32.98771181840748 34.0 33.0 34.0 31.0 34.0 4 36.392801752421995 37.0 37.0 37.0 35.0 37.0 5 36.373596261167854 37.0 37.0 37.0 35.0 37.0 6 36.43345685013487 37.0 37.0 37.0 35.0 37.0 7 36.403246170898484 37.0 37.0 37.0 35.0 37.0 8 36.33873935419543 37.0 37.0 37.0 35.0 37.0 9 38.1838058617354 39.0 39.0 39.0 37.0 39.0 10-11 38.20015616497272 39.0 39.0 39.0 37.0 39.0 12-13 38.195682973696385 39.0 39.0 39.0 37.0 39.0 14-15 39.759961437588984 41.0 40.0 41.0 37.0 41.0 16-17 39.777549098447196 41.0 40.0 41.0 37.0 41.0 18-19 39.801569359443576 41.0 40.0 41.0 37.5 41.0 20-21 39.81365909362776 41.0 40.0 41.0 37.5 41.0 22-23 39.7783387393542 41.0 40.0 41.0 37.0 41.0 24-25 39.76185688692024 41.0 40.0 41.0 37.0 41.0 26-27 39.688874049509515 41.0 40.0 41.0 37.0 41.0 28-29 39.62871769092958 41.0 40.0 41.0 37.0 41.0 30-31 39.51516100169614 41.0 40.0 41.0 36.5 41.0 32-33 39.390541042312165 41.0 39.5 41.0 35.5 41.0 34-35 39.29887355166236 41.0 39.0 41.0 35.0 41.0 36-37 39.20849130184957 41.0 39.0 41.0 35.0 41.0 38-39 39.11111118793937 41.0 39.0 41.0 35.0 41.0 40-41 39.00847411920812 41.0 39.0 41.0 35.0 41.0 42-43 38.8743437233574 41.0 39.0 41.0 35.0 41.0 44-45 38.70415916726766 41.0 39.0 41.0 35.0 41.0 46-47 38.48115700370688 41.0 39.0 41.0 35.0 41.0 48-49 38.270588745750146 41.0 38.0 41.0 34.5 41.0 50-51 38.118667055725 41.0 38.0 41.0 34.0 41.0 52-53 37.97748918392479 40.0 38.0 41.0 34.0 41.0 54-55 37.82835612943197 40.0 37.5 41.0 34.0 41.0 56-57 37.62748638353453 40.0 37.0 41.0 33.5 41.0 58-59 37.45918292909758 40.0 37.0 41.0 33.0 41.0 60-61 37.27115919561729 40.0 36.0 41.0 33.0 41.0 62-63 37.03645036360129 39.0 36.0 41.0 33.0 41.0 64-65 36.751737279140826 39.0 35.0 41.0 32.5 41.0 66-67 36.47956510285039 39.0 35.0 41.0 32.0 41.0 68-69 36.186233903806254 38.0 35.0 40.5 32.0 41.0 70-71 35.862676640665676 37.0 35.0 40.0 32.0 41.0 72-73 35.522402084873264 37.0 35.0 39.5 31.0 41.0 74-75 35.177309129203095 36.0 35.0 39.0 31.0 41.0 76-77 34.829405584323894 36.0 35.0 39.0 31.0 40.5 78-79 34.484494826884024 35.0 35.0 37.5 31.0 39.5 80-81 34.153727768877225 35.0 35.0 37.0 30.5 39.0 82-83 33.84438940083403 35.0 34.0 37.0 30.0 39.0 84-85 33.56552152601216 35.0 34.0 36.0 30.0 37.5 86-87 33.41261261198969 35.0 34.0 36.0 30.0 37.0 88-89 33.22223153764934 35.0 34.0 36.0 30.0 37.0 90-91 33.04989016247104 35.0 34.0 35.0 30.0 36.5 92-93 32.88871888714566 35.0 34.0 35.0 30.0 36.0 94-95 32.79019441622926 35.0 34.0 35.0 30.0 36.0 96-97 32.732667312946035 35.0 34.0 35.0 30.0 36.0 98-99 32.62473612372055 35.0 34.0 35.0 30.0 36.0 100 32.542206375624296 35.0 34.0 35.0 30.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19099474856660237 1101 2 0.18413290652492265 1101 3 0.20549673792547196 1101 4 0.16442936445631773 1101 5 0.1610996181679596 1101 6 0.14598008815519137 1101 7 0.1386195350474253 1101 8 0.05439315297072511 1101 9 0.12346101474603444 1101 10-11 0.19161792409106937 1101 12-13 0.19191293660288267 1101 14-15 0.27996492219946845 1101 16-17 0.27471878349010836 1101 18-19 0.26592451494261127 1101 20-21 0.24860491264814044 1101 22-23 0.2684372039191416 1101 24-25 0.2913508782645593 1101 26-27 0.2891861394635171 1101 28-29 0.23320461702694217 1101 30-31 0.272145278958547 1101 32-33 0.2542428525335154 1101 34-35 0.24441727254474443 1101 36-37 0.25408575869315086 1101 38-39 0.27639817727098404 1101 40-41 0.2542022297807165 1101 42-43 0.26038668143444 1101 44-45 0.2907322740734344 1101 46-47 0.32533949304314547 1101 48-49 0.27327092774985573 1101 50-51 0.2915199672920039 1101 52-53 0.2999444182347375 1101 54-55 0.3217043939533113 1101 56-57 0.2913384549112621 1101 58-59 0.2742732713068108 1101 60-61 0.2896069549991722 1101 62-63 0.26565217683226194 1101 64-65 0.26109629661475253 1101 66-67 0.2649389072688635 1101 68-69 0.19940870081067885 1101 70-71 0.25281136394428927 1101 72-73 0.195609994770912 1101 74-75 0.26531227037258276 1101 76-77 0.2501064835922051 1101 78-79 0.23434429512268196 1101 80-81 0.24500360852028535 1101 82-83 0.2531171529714058 1101 84-85 0.29283333125159317 1101 86-87 0.2803350714302866 1101 88-89 0.23505688841341765 1101 90-91 0.2843093724212338 1101 92-93 0.25819233017750065 1101 94-95 0.32598608436809684 1101 96-97 0.3259916453393146 1101 98-99 0.29682210762167216 1101 100 0.2712694907036024 1102 1 0.18952311979619907 1102 2 0.15416134103939072 1102 3 0.19482083135810058 1102 4 0.08126437259289787 1102 5 0.13958003662747132 1102 6 0.14162734058512427 1102 7 0.13433419221349396 1102 8 0.06948022867634052 1102 9 0.16062952268335096 1102 10-11 0.21371611457747264 1102 12-13 0.2377377181152056 1102 14-15 0.2308383308390063 1102 16-17 0.2894377971209252 1102 18-19 0.2800743054892081 1102 20-21 0.3083564543301307 1102 22-23 0.3257570330109658 1102 24-25 0.27100161765053343 1102 26-27 0.28704129969566594 1102 28-29 0.3092040455735514 1102 30-31 0.2959840047017437 1102 32-33 0.3084241795754039 1102 34-35 0.31989208408252523 1102 36-37 0.32609427547981085 1102 38-39 0.333779773386631 1102 40-41 0.3660591503420676 1102 42-43 0.34346394801626445 1102 44-45 0.3782874330479373 1102 46-47 0.33766644392858325 1102 48-49 0.3251450365189186 1102 50-51 0.32193901844767936 1102 52-53 0.32191089689299446 1102 54-55 0.24407603826769275 1102 56-57 0.2449136597321413 1102 58-59 0.2743672538295243 1102 60-61 0.2911976881861378 1102 62-63 0.2992813785464534 1102 64-65 0.3816538180385223 1102 66-67 0.3264844495452266 1102 68-69 0.27599872618596066 1102 70-71 0.28580251292183334 1102 72-73 0.29317144258251204 1102 74-75 0.3043866658100214 1102 76-77 0.3122830159766181 1102 78-79 0.30680135077848547 1102 80-81 0.3027979657154489 1102 82-83 0.2987863398193795 1102 84-85 0.3118349927659523 1102 86-87 0.2911494736664366 1102 88-89 0.2745444166476716 1102 90-91 0.26763521874872254 1102 92-93 0.27750338438695366 1102 94-95 0.28456463495439266 1102 96-97 0.30745679879443344 1102 98-99 0.25562505154830006 1102 100 0.2633756402768128 1103 1 0.17959167715826396 1103 2 0.19398829203564105 1103 3 0.19026245833009625 1103 4 0.09509344115557639 1103 5 0.11992774994046584 1103 6 0.1252491217561129 1103 7 0.10739231712555863 1103 8 0.05332657670997065 1103 9 0.1197656362881574 1103 10-11 0.14538069286782473 1103 12-13 0.17287496446343908 1103 14-15 0.1983193836815289 1103 16-17 0.24202329773041953 1103 18-19 0.24215247182974053 1103 20-21 0.24047150944900153 1103 22-23 0.2552850026585034 1103 24-25 0.21538911451737164 1103 26-27 0.2746415914535092 1103 28-29 0.3270022230003633 1103 30-31 0.2875272595805356 1103 32-33 0.3062776021218312 1103 34-35 0.2858631008695838 1103 36-37 0.26837217500244037 1103 38-39 0.3398716121600458 1103 40-41 0.3557516089486654 1103 42-43 0.33741750613255306 1103 44-45 0.28917805436093147 1103 46-47 0.29317131375232464 1103 48-49 0.25276496384927327 1103 50-51 0.22375151538465587 1103 52-53 0.26066855265610656 1103 54-55 0.26882981908840264 1103 56-57 0.2887231804717132 1103 58-59 0.30089046871249536 1103 60-61 0.26862439071425825 1103 62-63 0.23133915777358993 1103 64-65 0.2614822518207589 1103 66-67 0.31745807941427273 1103 68-69 0.27219935572531284 1103 70-71 0.2586928096216923 1103 72-73 0.18880267991667665 1103 74-75 0.21789609967768087 1103 76-77 0.27647907705474495 1103 78-79 0.26603458567129934 1103 80-81 0.2762337712601308 1103 82-83 0.26720019278921114 1103 84-85 0.2606715937500894 1103 86-87 0.3312204217522776 1103 88-89 0.2683988537444435 1103 90-91 0.26009109735940683 1103 92-93 0.3074111896016447 1103 94-95 0.28932530917789734 1103 96-97 0.224088888014208 1103 98-99 0.2742344334885942 1103 100 0.2474365266688423 1104 1 0.10019275955757934 1104 2 0.12446299707626451 1104 3 0.12085803315058286 1104 4 0.0829616299926812 1104 5 0.06563709315125266 1104 6 0.07590531010324497 1104 7 0.08068664965525585 1104 8 0.013919896531874087 1104 9 0.08289391554792047 1104 10-11 0.10141549942049721 1104 12-13 0.12027660059018075 1104 14-15 0.19213484127919855 1104 16-17 0.1674293018729358 1104 18-19 0.16704515640090278 1104 20-21 0.16415569921779394 1104 22-23 0.1566413135457836 1104 24-25 0.1185333875633674 1104 26-27 0.15154511648955804 1104 28-29 0.2070020211640653 1104 30-31 0.20318445478917369 1104 32-33 0.2530172384081908 1104 34-35 0.24576646536458924 1104 36-37 0.27858169847391423 1104 38-39 0.2954068738883606 1104 40-41 0.3453560538942213 1104 42-43 0.2541574544531784 1104 44-45 0.19832072222840935 1104 46-47 0.2874069736317253 1104 48-49 0.31429574365190405 1104 50-51 0.32168210759553517 1104 52-53 0.3044139881399488 1104 54-55 0.31648278779867667 1104 56-57 0.34616866031313265 1104 58-59 0.32590117531583473 1104 60-61 0.27475009319735477 1104 62-63 0.2496683291033719 1104 64-65 0.3114867583379848 1104 66-67 0.3516399255621252 1104 68-69 0.3308914356691872 1104 70-71 0.2795211260471717 1104 72-73 0.29854788711671176 1104 74-75 0.23861824905323914 1104 76-77 0.24510368335797494 1104 78-79 0.30317437748760767 1104 80-81 0.33946444183916924 1104 82-83 0.28159439739646785 1104 84-85 0.25484684773902444 1104 86-87 0.21437887089788177 1104 88-89 0.24298473555096933 1104 90-91 0.2809376544498434 1104 92-93 0.3033221197096765 1104 94-95 0.32317447343644545 1104 96-97 0.33713133465055023 1104 98-99 0.30596681062829845 1104 100 0.3335488789571599 1105 1 0.06896580540374941 1105 2 0.10173599828156199 1105 3 0.08765583235597063 1105 4 0.06873542357332241 1105 5 0.05789798552213199 1105 6 0.07496389007735615 1105 7 0.06178442430817199 1105 8 0.0051476319008259 1105 9 0.06073022865894018 1105 10-11 0.08946555856964267 1105 12-13 0.08901454755683602 1105 14-15 0.11662437299653305 1105 16-17 0.14294540606471173 1105 18-19 0.1159664378169154 1105 20-21 0.12020687996865576 1105 22-23 0.09733600909508766 1105 24-25 0.09806995959139186 1105 26-27 0.10166118273659919 1105 28-29 0.0951934523501734 1105 30-31 0.0965507665609735 1105 32-33 0.11939825934475579 1105 34-35 0.09144668053355076 1105 36-37 0.1207900541276743 1105 38-39 0.18122572684135463 1105 40-41 0.13944644048410737 1105 42-43 0.11882920459825641 1105 44-45 0.06267697306542885 1105 46-47 0.1704809993448606 1105 48-49 0.20017868414563367 1105 50-51 0.21792889903342427 1105 52-53 0.1340528156476566 1105 54-55 0.1094274108065747 1105 56-57 0.14272704498346656 1105 58-59 0.12405834463863386 1105 60-61 0.1475983756401007 1105 62-63 0.16654358020822713 1105 64-65 0.17624059187220098 1105 66-67 0.24279238527373792 1105 68-69 0.16146880944714326 1105 70-71 0.16805721260581663 1105 72-73 0.2104738893041187 1105 74-75 0.15919595763767802 1105 76-77 0.1624242828847855 1105 78-79 0.14270726664433653 1105 80-81 0.13665630827370734 1105 82-83 0.16991737962940334 1105 84-85 0.1275081965627436 1105 86-87 0.12508506146973275 1105 88-89 0.10929357779015447 1105 90-91 0.1255659076528275 1105 92-93 0.10514187664016816 1105 94-95 0.08004587426676579 1105 96-97 0.132227396210304 1105 98-99 0.17197100686953348 1105 100 0.11075207301099255 1106 1 0.025954893602893492 1106 2 -0.004018854125639848 1106 3 -7.274759798079344E-4 1106 4 -0.0032250950354466568 1106 5 -0.009403793258229598 1106 6 -0.0019404342984046252 1106 7 -0.028199960597859786 1106 8 -0.10108813859348231 1106 9 -0.09814435868036497 1106 10-11 -0.01289654272607521 1106 12-13 -0.06065986768134479 1106 14-15 0.002597300578692341 1106 16-17 -0.07051861153141914 1106 18-19 -0.028612524295368758 1106 20-21 -0.008490472703265084 1106 22-23 0.005181032303276822 1106 24-25 -0.012793508192586955 1106 26-27 -0.010575019350859804 1106 28-29 0.0030736999935072618 1106 30-31 0.058464225431620775 1106 32-33 0.0589377414061687 1106 34-35 0.0293308285994911 1106 36-37 0.03398340115940357 1106 38-39 0.02703076200547372 1106 40-41 0.06277284420063012 1106 42-43 0.06351314303324074 1106 44-45 0.09028609442486157 1106 46-47 0.04739910253314861 1106 48-49 0.052495602195214985 1106 50-51 0.027530881681229857 1106 52-53 -0.04479796705054895 1106 54-55 -0.004889311551693254 1106 56-57 0.05625151242833937 1106 58-59 0.03039003068008128 1106 60-61 -0.03787492254921432 1106 62-63 0.014560758151944242 1106 64-65 0.003719833179687271 1106 66-67 -0.028697669988950736 1106 68-69 0.06392117000703479 1106 70-71 0.12220727566934642 1106 72-73 0.038914457452271733 1106 74-75 0.11421732954097052 1106 76-77 0.0585535624593021 1106 78-79 -0.0367430820480763 1106 80-81 -0.022923866856615405 1106 82-83 0.04262653811657202 1106 84-85 0.031946341355535424 1106 86-87 0.040808107337895194 1106 88-89 0.0742794437844978 1106 90-91 0.04766958919604747 1106 92-93 0.050040244494262254 1106 94-95 0.06738831930900346 1106 96-97 0.031965491544141855 1106 98-99 0.01924361960313803 1106 100 0.1111197582106982 1107 1 0.03399062912557582 1107 2 0.05537594259180878 1107 3 0.07300302730845942 1107 4 0.03710676265280455 1107 5 0.028284257177602967 1107 6 0.04137149096214898 1107 7 0.007077939201749928 1107 8 0.03311356754029049 1107 9 0.06857883502676998 1107 10-11 0.05933980708187647 1107 12-13 0.07945613876071178 1107 14-15 0.09073178335678023 1107 16-17 0.09584440690164797 1107 18-19 0.09349067647898579 1107 20-21 0.07989855017212477 1107 22-23 0.11618202004529365 1107 24-25 0.10357012949010169 1107 26-27 0.12436890539316181 1107 28-29 0.14560674913875715 1107 30-31 0.14535712566470238 1107 32-33 0.13439012139494366 1107 34-35 0.16136606789334706 1107 36-37 0.10800785369480081 1107 38-39 0.07529857570553844 1107 40-41 0.06907056107648657 1107 42-43 0.07871629137088831 1107 44-45 0.10300817638459847 1107 46-47 0.1262264448034074 1107 48-49 0.08930548411337469 1107 50-51 0.18594071393505374 1107 52-53 0.19060060610502916 1107 54-55 0.23067622581266534 1107 56-57 0.23986050339397735 1107 58-59 0.185606763126259 1107 60-61 0.21630287484878608 1107 62-63 0.2759023150512192 1107 64-65 0.19754247873949282 1107 66-67 0.1552550174741114 1107 68-69 0.13429974502710706 1107 70-71 0.1628612208931628 1107 72-73 0.1809226731070126 1107 74-75 0.16204835201951795 1107 76-77 0.1674347214085543 1107 78-79 0.12821330697313726 1107 80-81 0.12972264281152945 1107 82-83 0.06885282208662602 1107 84-85 0.1450236031362948 1107 86-87 0.08297138931737891 1107 88-89 0.08571983447867382 1107 90-91 0.08907914851366172 1107 92-93 0.1290960872854825 1107 94-95 0.11043637686285024 1107 96-97 0.14754115351033192 1107 98-99 0.14630522208954488 1107 100 0.15968385306025823 1108 1 0.03107570215021127 1108 2 0.05544925984634119 1108 3 0.06698638293302395 1108 4 0.01610280337009584 1108 5 0.013224933353910728 1108 6 0.04184591448699848 1108 7 0.048639714473644347 1108 8 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-0.17959450951759237 2214 34-35 -0.18915420489266666 2214 36-37 -0.20552020731300047 2214 38-39 -0.15531363040131652 2214 40-41 -0.20377960196177725 2214 42-43 -0.19164639442880116 2214 44-45 -0.14786994934922149 2214 46-47 -0.1354033212716388 2214 48-49 -0.1901637716183373 2214 50-51 -0.21251560186753693 2214 52-53 -0.18054232062424092 2214 54-55 -0.1503087255923461 2214 56-57 -0.15982530666529726 2214 58-59 -0.13240369071255031 2214 60-61 -0.12365665251758173 2214 62-63 -0.16314456815946698 2214 64-65 -0.12937203180283774 2214 66-67 -0.21947184999934422 2214 68-69 -0.2192974464984303 2214 70-71 -0.1828971376033124 2214 72-73 -0.19837159864477627 2214 74-75 -0.2025114282848932 2214 76-77 -0.20711903101809526 2214 78-79 -0.2334925988009786 2214 80-81 -0.2507691103277736 2214 82-83 -0.23829897111605902 2214 84-85 -0.2515958151425792 2214 86-87 -0.2552588292304705 2214 88-89 -0.23062329000281778 2214 90-91 -0.21925157316372434 2214 92-93 -0.20501013250578382 2214 94-95 -0.24204936786108533 2214 96-97 -0.16177139467949075 2214 98-99 -0.12941714102769453 2214 100 -0.08843580013122931 2215 1 -0.16236535243088213 2215 2 -0.18457687819704205 2215 3 -0.17007395036002748 2215 4 -0.1255618105840881 2215 5 -0.14534898675471197 2215 6 -0.13252033408917185 2215 7 -0.17218174564658995 2215 8 -0.10095765899987441 2215 9 -0.18072321717148299 2215 10-11 -0.14754450629073546 2215 12-13 -0.18532799985735693 2215 14-15 -0.2534813155511202 2215 16-17 -0.2631744498113875 2215 18-19 -0.23997356373205747 2215 20-21 -0.23636991155220954 2215 22-23 -0.22654352237508846 2215 24-25 -0.23405346354773116 2215 26-27 -0.26603243029050105 2215 28-29 -0.2625490227015277 2215 30-31 -0.27140399878687305 2215 32-33 -0.24853010409530185 2215 34-35 -0.2497746042128739 2215 36-37 -0.2460407574997987 2215 38-39 -0.3092279721422173 2215 40-41 -0.1852967940270247 2215 42-43 -0.1968495983919496 2215 44-45 -0.2114080517123753 2215 46-47 -0.21827026208604394 2215 48-49 -0.27454585864706615 2215 50-51 -0.2185253386230528 2215 52-53 -0.2651087966257606 2215 54-55 -0.2653925903724925 2215 56-57 -0.3375635147161731 2215 58-59 -0.29295967316069493 2215 60-61 -0.2943782995921538 2215 62-63 -0.29749716010866223 2215 64-65 -0.269848122699365 2215 66-67 -0.23588807419343283 2215 68-69 -0.2607280743416922 2215 70-71 -0.2827099338958732 2215 72-73 -0.31048520276618063 2215 74-75 -0.30972876571448893 2215 76-77 -0.3087237955210185 2215 78-79 -0.2853921312210801 2215 80-81 -0.26316819533228397 2215 82-83 -0.32908986702383203 2215 84-85 -0.3372308765268528 2215 86-87 -0.36161073514772824 2215 88-89 -0.33673659945172574 2215 90-91 -0.32572594786724096 2215 92-93 -0.28593478714817877 2215 94-95 -0.32275635764231936 2215 96-97 -0.3185153597373471 2215 98-99 -0.34295756117375475 2215 100 -0.3873842343516003 2216 1 -0.20312668895284958 2216 2 -0.1977465102638405 2216 3 -0.21589136994669644 2216 4 -0.11115748979830187 2216 5 -0.11464099989348853 2216 6 -0.1483793720414539 2216 7 -0.11688047523753653 2216 8 -0.10968245739061899 2216 9 -0.1879234347924239 2216 10-11 -0.18820605671189838 2216 12-13 -0.24435442121313145 2216 14-15 -0.2946939465706109 2216 16-17 -0.27745212853106693 2216 18-19 -0.284356760491697 2216 20-21 -0.27129872286414525 2216 22-23 -0.34120302816209147 2216 24-25 -0.30094574952786957 2216 26-27 -0.2909120631873563 2216 28-29 -0.29283123775563524 2216 30-31 -0.3399929341825043 2216 32-33 -0.3698039103371755 2216 34-35 -0.3582381340596257 2216 36-37 -0.3535678712633086 2216 38-39 -0.36308764861887965 2216 40-41 -0.21766954598750488 2216 42-43 -0.19640799870742853 2216 44-45 -0.21369402766824663 2216 46-47 -0.23111103238217368 2216 48-49 -0.27412433427276284 2216 50-51 -0.1883387003305046 2216 52-53 -0.17917300280095816 2216 54-55 -0.15395572423279447 2216 56-57 -0.15085415861975093 2216 58-59 -0.17204621302326473 2216 60-61 -0.16675121002187154 2216 62-63 -0.21055737221136184 2216 64-65 -0.2147639284863203 2216 66-67 -0.2004379524063964 2216 68-69 -0.23905003439594452 2216 70-71 -0.2375857410557245 2216 72-73 -0.13352875236139994 2216 74-75 -0.1787653843374173 2216 76-77 -0.18206053035600434 2216 78-79 -0.1957225201628887 2216 80-81 -0.22231535806050573 2216 82-83 -0.17132267045405314 2216 84-85 -0.1525200232770274 2216 86-87 -0.13731468428123605 2216 88-89 -0.13625148548472765 2216 90-91 -0.18224111552598288 2216 92-93 -0.22266978973910767 2216 94-95 -0.19342722841670934 2216 96-97 -0.18544989910287768 2216 98-99 -0.18053281644541386 2216 100 -0.1384852813114108 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 3.0 4 10.0 5 21.0 6 83.0 7 155.0 8 300.0 9 589.0 10 1095.0 11 1725.0 12 2912.0 13 4634.0 14 5548.0 15 7311.0 16 9192.0 17 13365.0 18 16458.0 19 9026.0 20 5918.0 21 5516.0 22 5544.0 23 5851.0 24 6462.0 25 7675.0 26 9535.0 27 12265.0 28 15432.0 29 20440.0 30 25780.0 31 32985.0 32 42336.0 33 55529.0 34 78320.0 35 121884.0 36 230930.0 37 549748.0 38 1030008.0 39 555355.0 40 2505.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48771577303524 19.666379597358503 13.316086126324095 23.529818503282165 2 15.736245940706747 20.141942590279406 40.25681458230551 23.864996886708337 3 18.293626510397758 25.504053656934435 30.1467268232813 26.055593009386506 4 12.212282957732405 16.64301044112608 38.8559468984873 32.28875970265421 5 12.343881647206146 38.08570420029892 35.7938306002862 13.776583552208743 6 30.6681275459648 38.30700731108085 17.557726975074004 13.467138167880341 7 27.300300854715037 32.81864427992281 22.355593539817438 17.52546132554472 8 25.46533701856778 36.07624322131869 20.086120369783707 18.37229939032983 9 25.429968974055615 16.188635252581058 21.17283878056353 37.2085569927998 10-11 23.935073816212807 26.681098472093247 28.756965538604934 20.626862173089012 12-13 24.997817595967437 24.047222901672544 29.116830786330976 21.838128716029047 14-15 21.950853125505084 25.88198077582951 26.648613935751754 25.518552162913654 16-17 21.052953651121157 29.48997633151642 27.180898987503344 22.276171029859075 18-19 20.98355702197842 27.796828329473573 30.04988330828825 21.169731340259755 20-21 21.95972693083451 27.169455417441384 29.845764185013834 21.025053466710276 22-23 21.62533492368062 26.947224671126552 29.957924942522773 21.469515462670056 24-25 21.444029205085048 27.058179050466535 29.851839700128767 21.645952044319653 26-27 21.156499429431054 27.10943254796332 29.990663102937344 21.743404919668283 28-29 21.267720536105266 27.149893481864602 30.077651141567035 21.504734840463097 30-31 21.46569278150964 27.512042458331592 29.639246640542254 21.38301811961651 32-33 21.258062820671526 27.19355057334461 30.04907022114276 21.4993163848411 34-35 21.370628267878786 26.99051090738162 30.0451276131575 21.59373321158209 36-37 21.686533220743993 27.362651178080977 29.43190399862209 21.51891160255294 38-39 21.475114364831597 27.5573415200544 29.552301373899848 21.41524274121416 40-41 21.307705732050685 27.227296965260805 29.77634712937392 21.688650173314585 42-43 21.382704294587203 27.214984693821066 29.53497448047563 21.867336531116106 44-45 21.488659551885426 27.472531276072772 29.377088309206567 21.66172086283524 46-47 21.659318207189347 27.693806784697784 28.97376275451703 21.673112253595843 48-49 21.641169935727575 27.56800248387824 28.869927126072227 21.92090045432196 50-51 21.701233064530363 27.61477143671073 28.654342136552646 22.029653362206258 52-53 21.732513459438756 27.667005809853336 28.61387812685663 21.98660260385128 54-55 21.78568752095285 28.03584653330571 28.456989127276174 21.72147681846527 56-57 21.576407358251902 27.755182909419997 28.53168924519258 22.136720487135516 58-59 21.51274183744288 27.33862493461632 28.934306938136782 22.21432628980402 60-61 21.772513867339004 27.694153560675154 28.54504590319778 21.988286668788056 62-63 21.526818179144072 27.59337334491946 28.67912279044304 22.20068568549343 64-65 21.530800489826284 27.462251776519174 28.770241462785577 22.236706270868957 66-67 21.399943957573704 27.556901126950617 28.64409308159532 22.39906183388036 68-69 21.781382872890262 27.73274001845149 28.51427483867912 21.97160226997913 70-71 21.77082911978172 27.471958922063727 28.50018651988468 22.25702543826987 72-73 21.559098260508204 27.64469201584537 28.54925264152861 22.246957082117813 74-75 21.6438878543963 27.72567861061922 28.414159741174792 22.216273793809684 76-77 21.692609778456017 27.673420858887642 28.232842705519072 22.40112665713726 78-79 21.75810259352484 27.84891788234452 28.308320254759494 22.08465926937114 80-81 21.64842489712859 27.696316543161046 28.3725435150734 22.282715044636962 82-83 21.531719314670717 27.752606873967355 28.439913720172765 22.275760091189163 84-85 21.474066657585567 27.657432062878094 28.30444321672877 22.564058062807568 86-87 21.662473826078102 27.71850525016936 28.228820018320235 22.3902009054323 88-89 21.35844406879102 27.72374134446626 28.33633800372454 22.581476583018187 90-91 21.39804245637308 27.626184916151576 28.31836592592823 22.657406701547114 92-93 21.51052307775283 27.790643744521255 28.130012548279065 22.568820629446847 94-95 21.295627084428506 27.545667059473704 28.31413868514341 22.844567170954384 96-97 21.353575688430222 27.531469861190338 28.243772580338465 22.871181870040967 98-99 21.251066352861457 27.674456192452602 28.16518162355302 22.909295831132923 100 21.322344279286725 27.61098361460545 28.087291967305273 22.97938013880255 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 54.0 1 67.0 2 225.0 3 385.0 4 260.5 5 166.0 6 485.5 7 1170.5 8 1934.0 9 2201.5 10 1743.5 11 1292.5 12 1320.5 13 1538.5 14 1743.5 15 1927.5 16 2133.5 17 2429.0 18 2772.0 19 3166.0 20 3777.5 21 4682.5 22 6074.0 23 8292.0 24 11003.0 25 14573.5 26 19419.0 27 25324.0 28 32817.0 29 40709.5 30 47775.0 31 54907.5 32 62440.5 33 69843.5 34 77539.5 35 84040.5 36 88490.5 37 93203.0 38 97051.0 39 98522.5 40 100008.5 41 101830.5 42 102110.5 43 102121.5 44 101572.0 45 100726.0 46 100339.5 47 97573.0 48 94232.0 49 91552.5 50 89207.0 51 86623.0 52 80689.5 53 75743.5 54 73403.0 55 70605.5 56 65197.0 57 58442.5 58 52994.5 59 48465.0 60 43537.0 61 37162.5 62 31712.5 63 27286.5 64 22363.5 65 17644.5 66 13712.5 67 10207.5 68 7710.5 69 5827.0 70 4110.0 71 3013.0 72 2320.0 73 1614.5 74 1122.5 75 800.5 76 495.5 77 344.5 78 251.0 79 149.5 80 82.5 81 40.5 82 18.5 83 9.0 84 3.5 85 1.0 86 1.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.7286359610213335E-4 2 5.877362267472534E-4 3 1.3829087688170668E-4 4 0.01151271550040208 5 5.877362267472534E-4 6 0.00456359893709632 7 9.680361381719467E-4 8 0.14420281186839964 9 0.0012446178919353602 10-11 0.0 12-13 5.185907883064E-5 14-15 5.185907883064E-5 16-17 0.0 18-19 1.2100451727149333E-4 20-21 0.0 22-23 1.3829087688170668E-4 24-25 4.321589902553334E-4 26-27 0.005721785030980614 28-29 0.007727002745765361 30-31 0.013656224092068535 32-33 0.016456614348923093 34-35 0.026465416563236612 36-37 0.03873873188648808 38-39 0.043838207971501016 40-41 0.055748509742938004 42-43 0.07322501930886369 44-45 0.11424555066389994 46-47 0.12192069433083463 48-49 0.1183597042511307 50-51 0.12007105385254183 52-53 0.11445298697922249 54-55 0.12195526705005508 56-57 0.10057204021222117 58-59 0.09612944579239635 60-61 0.09820380894562195 62-63 0.09649245934421083 64-65 0.0 66-67 8.643179805106668E-5 68-69 1.5557723649192002E-4 70-71 3.457271922042667E-5 72-73 0.0 74-75 0.0 76-77 1.2100451727149333E-4 78-79 1.7286359610213335E-5 80-81 2.7658175376341337E-4 82-83 1.7286359610213335E-4 84-85 0.0 86-87 8.124589016800267E-4 88-89 4.321589902553334E-4 90-91 8.643179805106668E-5 92-93 8.816043401208799E-4 94-95 8.470316209004534E-4 96-97 1.3829087688170668E-4 98-99 5.185907883064E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2892454.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.20562258577155 #Duplication Level Percentage of deduplicated Percentage of total 1 77.85247409327174 48.42861620814619 2 13.525565536725562 16.82732450073338 3 3.9603367871329045 7.390656464788097 4 1.6262032327327633 4.046359381725436 5 0.8508624203348975 2.6464213295884367 6 0.5038073121471799 1.8803788509227684 7 0.3170394410284018 1.3805145069391718 8 0.22550898744374037 1.1222341570099845 9 0.170663338498054 0.9554597301453952 >10 0.8900927086967894 9.930007081098042 >50 0.053923040238920056 2.291661417684521 >100 0.022744017726990827 2.28102324863703 >500 5.008397286438917E-4 0.19215740737458853 >1k 1.6694657611894615E-4 0.19748838200989768 >5k 1.1129771741263077E-4 0.4296973331969956 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6863 0.23727257200978824 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5550 0.19187859167336802 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3121 0.10790145668695163 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.457271922042667E-5 0.0 3 0.0 0.0 0.0 3.457271922042667E-5 0.0 4 0.0 0.0 0.0 3.457271922042667E-5 0.0 5 0.0 0.0 0.0 3.457271922042667E-5 0.0 6 0.0 0.0 0.0 3.457271922042667E-5 0.0 7 0.0 0.0 0.0 3.457271922042667E-5 0.0 8 0.0 0.0 0.0 3.457271922042667E-5 0.0 9 0.0 0.0 0.0 1.7286359610213335E-4 0.0 10-11 0.0 0.0 0.0 1.7286359610213335E-4 0.0 12-13 0.0 0.0 0.0 1.7286359610213335E-4 0.0 14-15 0.0 0.0 0.0 1.7286359610213335E-4 0.0 16-17 0.0 0.0 0.0 2.2472267493277336E-4 0.0 18-19 0.0 0.0 0.0 2.938681133736267E-4 0.0 20-21 0.0 0.0 0.0 3.802999114246934E-4 0.0 22-23 0.0 0.0 0.0 4.840180690859734E-4 0.0 24-25 0.0 0.0 0.0 5.877362267472534E-4 0.0 26-27 0.0 0.0 0.0 8.643179805106667E-4 0.0 28-29 0.0 0.0 0.0 0.0022990858281583737 0.0 30-31 0.0 0.0 0.0 0.0066033893711014945 0.0 32-33 0.0 0.0 0.0 0.015782446324124774 0.0 34-35 0.0 0.0 0.0 0.028366916120360082 0.0 36-37 1.7286359610213335E-5 0.0 0.0 0.05348399663400005 0.0 38-39 3.457271922042667E-5 0.0 0.0 0.0973567773247215 0.0 40-41 3.457271922042667E-5 0.0 0.0 0.15001102869743133 0.0 42-43 3.457271922042667E-5 0.0 0.0 0.20302829362195562 0.0 44-45 3.457271922042667E-5 0.0 0.0 0.2652246154995032 0.0 46-47 3.457271922042667E-5 0.0 0.0 0.3421316294053423 0.0 48-49 3.457271922042667E-5 0.0 0.0 0.43191698122079036 0.0 50-51 3.457271922042667E-5 0.0 0.0 0.5295330539396651 0.0 52-53 3.457271922042667E-5 0.0 0.0 0.6263020950376392 0.0 54-55 3.457271922042667E-5 0.0 0.0 0.7320427567733143 0.0 56-57 3.457271922042667E-5 0.0 0.0 0.8611545767019977 0.0 58-59 3.457271922042667E-5 0.0 0.0 1.0092641058423055 0.0 60-61 3.457271922042667E-5 0.0 0.0 1.1723436224050583 0.0 62-63 3.457271922042667E-5 0.0 0.0 1.333417921253026 0.0 64-65 3.457271922042667E-5 0.0 0.0 1.5035675588963557 0.0 66-67 3.457271922042667E-5 0.0 0.0 1.6966561957424386 0.0 68-69 3.457271922042667E-5 0.0 0.0 1.9174030079648632 0.0 70-71 3.457271922042667E-5 0.0 0.0 2.1625754463165188 0.0 72-73 3.457271922042667E-5 0.0 0.0 2.4117237473785234 0.0 74-75 3.457271922042667E-5 0.0 0.0 2.659938585021577 0.0 76-77 3.457271922042667E-5 0.0 0.0 2.9249730505653675 0.0 78-79 3.457271922042667E-5 0.0 0.0 3.2308378975084824 0.0 80-81 3.457271922042667E-5 0.0 0.0 3.561404952334592 0.0 82-83 3.457271922042667E-5 0.0 0.0 3.9039514543705796 0.0 84-85 3.457271922042667E-5 0.0 0.0 4.242089934705962 0.0 86-87 3.457271922042667E-5 0.0 0.0 4.594869961631197 0.0 88 3.457271922042667E-5 0.0 0.0 4.881771672081907 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4320 0.0 54.151226 1 GTATCAA 8050 0.0 43.82347 1 TCAACGC 9760 0.0 35.38346 4 ATCAACG 9790 0.0 35.275032 3 CAACGCA 9905 0.0 34.908745 5 TATCAAC 10345 0.0 33.65369 2 AACGCAG 10345 0.0 33.423985 6 TAGACCG 125 2.3461143E-7 30.107965 5 ACGCAGA 12080 0.0 28.350344 7 CGCAGAG 12245 0.0 27.929426 8 GTACATG 9000 0.0 26.357946 1 TACATGG 9080 0.0 24.81882 2 GCAGAGT 13730 0.0 24.767702 9 GTACTAG 545 0.0 24.133718 1 TACACCG 470 0.0 24.022312 5 ACATGGG 9235 0.0 23.537436 3 CGCGCAC 270 4.7293724E-11 22.613861 94 GTGGTAT 2090 0.0 22.026348 1 TATAACG 220 6.248956E-8 21.38498 2 GAGTACT 9010 0.0 21.29462 12-13 >>END_MODULE