##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032991_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1304 Sequences flagged as poor quality 0 Sequence length 100 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 17.479294478527606 24.0 2.0 30.0 2.0 31.0 2 17.521472392638035 23.0 2.0 30.0 2.0 31.0 3 17.4409509202454 23.0 2.0 30.0 2.0 31.0 4 19.20782208588957 25.0 2.0 35.0 2.0 37.0 5 19.0920245398773 24.0 2.0 35.0 2.0 37.0 6 18.970092024539877 23.0 2.0 35.0 2.0 37.0 7 18.76840490797546 21.0 2.0 35.0 2.0 37.0 8 18.907208588957054 23.0 2.0 35.0 2.0 37.0 9 19.471625766871167 25.0 2.0 35.0 2.0 39.0 10-11 19.45245398773006 24.0 2.0 35.0 2.0 39.0 12-13 19.370398773006137 24.5 2.0 35.0 2.0 39.0 14-15 19.689800613496935 25.0 2.0 36.0 2.0 40.0 16-17 19.28144171779141 20.5 2.0 36.0 2.0 40.0 18-19 18.911042944785276 17.5 2.0 36.0 2.0 40.0 20-21 18.73696319018405 18.0 2.0 36.0 2.0 40.0 22-23 18.289110429447852 15.5 2.0 36.0 2.0 40.0 24-25 18.054831288343557 13.5 2.0 36.0 2.0 40.0 26-27 17.726610429447852 10.0 2.0 36.0 2.0 40.0 28-29 17.34394171779141 8.0 2.0 35.5 2.0 40.0 30-31 17.234662576687114 5.0 2.0 35.5 2.0 40.0 32-33 16.913726993865033 2.0 2.0 35.0 2.0 40.0 34-35 16.63765337423313 2.0 2.0 34.5 2.0 40.0 36-37 16.50153374233129 2.0 2.0 35.0 2.0 39.5 38-39 16.32898773006135 2.0 2.0 35.0 2.0 40.0 40-41 16.34355828220859 2.0 2.0 34.5 2.0 40.0 42-43 16.201303680981596 2.0 2.0 34.5 2.0 39.0 44-45 16.14455521472393 2.0 2.0 34.5 2.0 39.0 46-47 15.776073619631902 2.0 2.0 34.0 2.0 39.0 48-49 15.614647239263803 2.0 2.0 33.5 2.0 39.0 50-51 15.460122699386503 2.0 2.0 33.5 2.0 39.0 52-53 15.295628834355828 2.0 2.0 33.5 2.0 39.0 54-55 14.98926380368098 2.0 2.0 33.0 2.0 38.5 56-57 14.914493865030675 2.0 2.0 33.0 2.0 39.0 58-59 14.506134969325153 2.0 2.0 32.0 2.0 38.0 60-61 14.327837423312882 2.0 2.0 32.5 2.0 38.5 62-63 14.061733128834355 2.0 2.0 31.5 2.0 38.0 64-65 13.795245398773005 2.0 2.0 31.0 2.0 37.5 66-67 13.679447852760736 2.0 2.0 32.0 2.0 37.0 68-69 13.44823619631902 2.0 2.0 31.0 2.0 37.0 70-71 13.308665644171779 2.0 2.0 31.0 2.0 36.5 72-73 13.24463190184049 2.0 2.0 31.0 2.0 36.0 74-75 13.164493865030675 2.0 2.0 31.0 2.0 36.0 76-77 12.869631901840492 2.0 2.0 30.0 2.0 35.0 78-79 12.544861963190185 2.0 2.0 29.5 2.0 35.0 80-81 12.324769938650306 2.0 2.0 29.0 2.0 35.0 82-83 12.043711656441719 2.0 2.0 29.0 2.0 35.0 84-85 12.054831288343557 2.0 2.0 29.0 2.0 35.0 86-87 11.86771472392638 2.0 2.0 29.0 2.0 34.5 88-89 11.748466257668712 2.0 2.0 29.0 2.0 34.5 90-91 11.428680981595093 2.0 2.0 29.0 2.0 34.0 92-93 11.201687116564418 2.0 2.0 29.0 2.0 34.0 94-95 10.94478527607362 2.0 2.0 27.0 2.0 34.0 96-97 10.800996932515337 2.0 2.0 27.0 2.0 34.0 98-99 10.73888036809816 2.0 2.0 29.0 2.0 34.0 100 10.601993865030675 2.0 2.0 27.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1102 1 -14.438524590163933 1102 2 -14.173497267759561 1102 3 -14.25 1102 4 -16.329234972677593 1102 5 -15.975409836065573 1102 6 -16.737704918032787 1102 7 -16.77459016393443 1102 8 -16.228142076502735 1102 9 -16.5 1102 10-11 -17.079234972677593 1102 12-13 -17.07103825136612 1102 14-15 -16.97267759562842 1102 16-17 -16.27732240437158 1102 18-19 -15.803278688524589 1102 20-21 -15.539617486338795 1102 22-23 -15.325819672131146 1102 24-25 -15.074453551912566 1102 26-27 -15.394808743169403 1102 28-29 -14.229508196721312 1102 30-31 -14.40983606557377 1102 32-33 -13.741120218579235 1102 34-35 -13.900956284153006 1102 36-37 -14.052595628415304 1102 38-39 -14.592213114754099 1102 40-41 -14.280054644808743 1102 42-43 -14.325819672131146 1102 44-45 -14.35928961748634 1102 46-47 -14.581284153005463 1102 48-49 -14.189207650273229 1102 50-51 -13.392759562841531 1102 52-53 -13.55327868852459 1102 54-55 -12.913251366120218 1102 56-57 -12.681010928961747 1102 58-59 -12.713114754098363 1102 60-61 -12.271857923497267 1102 62-63 -11.152322404371587 1102 64-65 -11.264344262295085 1102 66-67 -11.51639344262295 1102 68-69 -11.407103825136613 1102 70-71 -11.247950819672132 1102 72-73 -11.012978142076504 1102 74-75 -11.32103825136612 1102 76-77 -11.183060109289617 1102 78-79 -10.705601092896172 1102 80-81 -9.776639344262296 1102 82-83 -9.775273224043715 1102 84-85 -9.683743169398907 1102 86-87 -9.422814207650275 1102 88-89 -9.489071038251367 1102 90-91 -9.584699453551913 1102 92-93 -8.94808743169399 1102 94-95 -8.276639344262296 1102 96-97 -8.154371584699453 1102 98-99 -8.165983606557377 1102 100 -8.368852459016393 1103 1 -2.438524590163933 1103 2 -2.6734972677595614 1103 3 -2.25 1103 4 -4.329234972677593 1103 5 -13.475409836065573 1103 6 -11.237704918032787 1103 7 -2.2745901639344304 1103 8 -2.728142076502735 1103 9 -13.5 1103 10-11 -8.329234972677593 1103 12-13 -10.321038251366119 1103 14-15 -8.722677595628419 1103 16-17 -2.5273224043715814 1103 18-19 -11.553278688524589 1103 20-21 -12.539617486338795 1103 22-23 -7.325819672131146 1103 24-25 -10.824453551912566 1103 26-27 -11.144808743169403 1103 28-29 -3.479508196721312 1103 30-31 -0.6598360655737707 1103 32-33 0.25887978142076484 1103 34-35 -0.15095628415300588 1103 36-37 -1.302595628415304 1103 38-39 -0.5922131147540988 1103 40-41 -3.030054644808743 1103 42-43 -1.0758196721311464 1103 44-45 -0.8592896174863398 1103 46-47 -2.5812841530054627 1103 48-49 -3.6892076502732287 1103 50-51 -2.3927595628415315 1103 52-53 -0.053278688524590834 1103 54-55 -5.1632513661202175 1103 56-57 0.0689890710382528 1103 58-59 1.2868852459016367 1103 60-61 -5.021857923497267 1103 62-63 -0.6523224043715867 1103 64-65 1.4856557377049153 1103 66-67 0.7336065573770494 1103 68-69 0.3428961748633874 1103 70-71 2.2520491803278677 1103 72-73 1.4870218579234962 1103 74-75 -0.8210382513661205 1103 76-77 0.06693989071038331 1103 78-79 -0.7056010928961722 1103 80-81 2.2233606557377037 1103 82-83 2.4747267759562845 1103 84-85 3.316256830601093 1103 86-87 3.3271857923497254 1103 88-89 0.7609289617486326 1103 90-91 0.165300546448087 1103 92-93 0.30191256830601 1103 94-95 4.473360655737704 1103 96-97 4.845628415300547 1103 98-99 1.3340163934426226 1103 100 3.1311475409836067 1104 1 1.561475409836067 1104 2 0.8265027322404386 1104 3 -0.25 1104 4 -3.329234972677593 1104 5 1.5245901639344268 1104 6 7.262295081967213 1104 7 14.22540983606557 1104 8 11.271857923497265 1104 9 8.0 1104 10-11 12.170765027322407 1104 12-13 7.428961748633881 1104 14-15 10.277322404371581 1104 16-17 1.7226775956284186 1104 18-19 0.6967213114754109 1104 20-21 -0.5396174863387948 1104 22-23 -2.5758196721311464 1104 24-25 -2.3244535519125655 1104 26-27 -0.39480874316940273 1104 28-29 -14.229508196721312 1104 30-31 -14.40983606557377 1104 32-33 -13.741120218579235 1104 34-35 -13.900956284153006 1104 36-37 -14.052595628415304 1104 38-39 -14.592213114754099 1104 40-41 -14.280054644808743 1104 42-43 -14.325819672131146 1104 44-45 -14.35928961748634 1104 46-47 -14.581284153005463 1104 48-49 -14.189207650273229 1104 50-51 -13.392759562841531 1104 52-53 -13.55327868852459 1104 54-55 -12.913251366120218 1104 56-57 -12.681010928961747 1104 58-59 -12.713114754098363 1104 60-61 -12.271857923497267 1104 62-63 -11.152322404371587 1104 64-65 -11.264344262295085 1104 66-67 -11.51639344262295 1104 68-69 -11.407103825136613 1104 70-71 -11.247950819672132 1104 72-73 -11.012978142076504 1104 74-75 -11.32103825136612 1104 76-77 -11.183060109289617 1104 78-79 -10.705601092896172 1104 80-81 -9.776639344262296 1104 82-83 -9.775273224043715 1104 84-85 -9.683743169398907 1104 86-87 -9.422814207650275 1104 88-89 -9.489071038251367 1104 90-91 -9.584699453551913 1104 92-93 -8.94808743169399 1104 94-95 -8.276639344262296 1104 96-97 -8.154371584699453 1104 98-99 -8.165983606557377 1104 100 -8.368852459016393 1105 1 -14.438524590163933 1105 2 -14.173497267759561 1105 3 -14.25 1105 4 -16.329234972677593 1105 5 -15.975409836065573 1105 6 -16.737704918032787 1105 7 -16.77459016393443 1105 8 -16.228142076502735 1105 9 -16.5 1105 10-11 -17.079234972677593 1105 12-13 -17.07103825136612 1105 14-15 -16.97267759562842 1105 16-17 -16.27732240437158 1105 18-19 -15.803278688524589 1105 20-21 -15.539617486338795 1105 22-23 -15.325819672131146 1105 24-25 -15.074453551912566 1105 26-27 -15.394808743169403 1105 28-29 -14.229508196721312 1105 30-31 -14.40983606557377 1105 32-33 -13.741120218579235 1105 34-35 -13.900956284153006 1105 36-37 -14.052595628415304 1105 38-39 -14.592213114754099 1105 40-41 -14.280054644808743 1105 42-43 -14.325819672131146 1105 44-45 -14.35928961748634 1105 46-47 -14.581284153005463 1105 48-49 -14.189207650273229 1105 50-51 -13.392759562841531 1105 52-53 -13.55327868852459 1105 54-55 -12.913251366120218 1105 56-57 -12.681010928961747 1105 58-59 -12.713114754098363 1105 60-61 -12.271857923497267 1105 62-63 -11.152322404371587 1105 64-65 -11.264344262295085 1105 66-67 -11.51639344262295 1105 68-69 -11.407103825136613 1105 70-71 -11.247950819672132 1105 72-73 -11.012978142076504 1105 74-75 -11.32103825136612 1105 76-77 -11.183060109289617 1105 78-79 -10.705601092896172 1105 80-81 -9.776639344262296 1105 82-83 -9.775273224043715 1105 84-85 -9.683743169398907 1105 86-87 -9.422814207650275 1105 88-89 -9.489071038251367 1105 90-91 -9.584699453551913 1105 92-93 -8.94808743169399 1105 94-95 -8.276639344262296 1105 96-97 -8.154371584699453 1105 98-99 -8.165983606557377 1105 100 -8.368852459016393 1106 1 -0.43852459016393297 1106 2 0.3265027322404386 1106 3 -0.25 1106 4 0.17076502732240684 1106 5 0.5245901639344268 1106 6 0.2622950819672134 1106 7 -0.27459016393443036 1106 8 1.2718579234972651 1106 9 1.5 1106 10-11 1.1707650273224068 1106 12-13 1.4289617486338813 1106 14-15 1.5273224043715814 1106 16-17 2.2226775956284186 1106 18-19 2.696721311475411 1106 20-21 2.960382513661205 1106 22-23 3.1741803278688536 1106 24-25 2.1755464480874345 1106 26-27 3.1051912568305973 1106 28-29 4.770491803278688 1106 30-31 4.340163934426229 1106 32-33 3.758879781420765 1106 34-35 5.099043715846994 1106 36-37 5.447404371584696 1106 38-39 3.6577868852459012 1106 40-41 4.969945355191257 1106 42-43 4.924180327868854 1106 44-45 5.14071038251366 1106 46-47 4.668715846994537 1106 48-49 5.310792349726771 1106 50-51 6.1072404371584685 1106 52-53 4.696721311475409 1106 54-55 5.8367486338797825 1106 56-57 6.568989071038253 1106 58-59 4.786885245901637 1106 60-61 5.728142076502733 1106 62-63 3.0976775956284133 1106 64-65 6.485655737704915 1106 66-67 6.233606557377049 1106 68-69 6.092896174863387 1106 70-71 5.752049180327868 1106 72-73 4.987021857923496 1106 74-75 5.4289617486338795 1106 76-77 5.066939890710383 1106 78-79 5.294398907103828 1106 80-81 6.723360655737704 1106 82-83 6.7247267759562845 1106 84-85 6.066256830601093 1106 86-87 6.5771857923497254 1106 88-89 6.010928961748633 1106 90-91 6.915300546448087 1106 92-93 7.30191256830601 1106 94-95 7.973360655737704 1106 96-97 8.345628415300547 1106 98-99 5.834016393442623 1106 100 7.131147540983607 1107 1 -0.43852459016393297 1107 2 -1.1734972677595614 1107 3 0.25 1107 4 0.17076502732240684 1107 5 0.5245901639344268 1107 6 -0.2377049180327866 1107 7 -0.27459016393443036 1107 8 0.2718579234972651 1107 9 -1.0 1107 10-11 -0.8292349726775932 1107 12-13 -1.3210382513661187 1107 14-15 -2.4726775956284186 1107 16-17 -1.5273224043715814 1107 18-19 0.19672131147541094 1107 20-21 1.4603825136612052 1107 22-23 -3.3258196721311464 1107 24-25 -4.5744535519125655 1107 26-27 0.10519125683059727 1107 28-29 3.520491803278688 1107 30-31 3.3401639344262293 1107 32-33 1.0088797814207648 1107 34-35 3.599043715846994 1107 36-37 -9.802595628415304 1107 38-39 -2.5922131147540988 1107 40-41 -5.280054644808743 1107 42-43 1.4241803278688536 1107 44-45 1.6407103825136602 1107 46-47 -1.5812841530054627 1107 48-49 -0.4392076502732287 1107 50-51 -9.392759562841531 1107 52-53 -3.553278688524591 1107 54-55 -2.1632513661202175 1107 56-57 -0.4310109289617472 1107 58-59 0.7868852459016367 1107 60-61 1.978142076502733 1107 62-63 -0.15232240437158673 1107 64-65 -4.264344262295085 1107 66-67 -4.266393442622951 1107 68-69 -6.407103825136613 1107 70-71 -2.2479508196721323 1107 72-73 -6.262978142076504 1107 74-75 0.6789617486338795 1107 76-77 2.8169398907103833 1107 78-79 3.0443989071038278 1107 80-81 -1.2766393442622963 1107 82-83 0.9747267759562845 1107 84-85 -2.183743169398907 1107 86-87 -3.9228142076502746 1107 88-89 0.5109289617486326 1107 90-91 -0.584699453551913 1107 92-93 1.05191256830601 1107 94-95 1.7233606557377037 1107 96-97 5.595628415300547 1107 98-99 2.8340163934426226 1107 100 5.631147540983607 1108 1 14.228142076502735 1108 2 15.15983606557377 1108 3 11.75 1108 4 16.670765027322407 1108 5 17.024590163934427 1108 6 16.928961748633878 1108 7 16.558743169398905 1108 8 17.43852459016393 1108 9 12.5 1108 10-11 15.754098360655735 1108 12-13 16.42896174863388 1108 14-15 18.693989071038253 1108 16-17 20.72267759562842 1108 18-19 19.69672131147541 1108 20-21 19.960382513661205 1108 22-23 19.840846994535525 1108 24-25 20.758879781420763 1108 26-27 16.27185792349727 1108 28-29 18.93715846994536 1108 30-31 17.423497267759565 1108 32-33 15.0922131147541 1108 34-35 21.265710382513667 1108 36-37 21.114071038251367 1108 38-39 19.4077868852459 1108 40-41 21.719945355191257 1108 42-43 21.507513661202182 1108 44-45 21.80737704918033 1108 46-47 21.5853825136612 1108 48-49 20.977459016393443 1108 50-51 21.273907103825138 1108 52-53 19.11338797814208 1108 54-55 14.586748633879782 1108 56-57 18.485655737704924 1108 58-59 22.786885245901637 1108 60-61 19.3948087431694 1108 62-63 22.014344262295076 1108 64-65 23.40232240437159 1108 66-67 19.98360655737705 1108 68-69 24.25956284153006 1108 70-71 22.085382513661195 1108 72-73 20.15368852459016 1108 74-75 23.67896174863388 1108 76-77 17.65027322404372 1108 78-79 19.794398907103826 1108 80-81 22.723360655737704 1108 82-83 12.224726775956285 1108 84-85 11.649590163934429 1108 86-87 10.410519125683058 1108 88-89 11.844262295081968 1108 90-91 12.081967213114751 1108 92-93 13.55191256830601 1108 94-95 12.55669398907104 1108 96-97 13.178961748633883 1108 98-99 13.00068306010929 1108 100 12.631147540983607 1109 1 -0.43852459016393297 1109 2 -1.6734972677595614 1109 3 -2.25 1109 4 -0.8292349726775932 1109 5 0.5245901639344268 1109 6 -1.2377049180327866 1109 7 -0.27459016393443036 1109 8 -2.228142076502735 1109 9 0.0 1109 10-11 0.9207650273224068 1109 12-13 0.9289617486338813 1109 14-15 -0.2226775956284186 1109 16-17 0.7226775956284186 1109 18-19 1.696721311475411 1109 20-21 0.4603825136612052 1109 22-23 1.4241803278688536 1109 24-25 2.6755464480874345 1109 26-27 1.6051912568305973 1109 28-29 3.020491803278688 1109 30-31 2.8401639344262293 1109 32-33 1.7588797814207648 1109 34-35 2.349043715846994 1109 36-37 3.947404371584696 1109 38-39 3.4077868852459012 1109 40-41 -2.780054644808743 1109 42-43 0.9241803278688536 1109 44-45 -0.3592896174863398 1109 46-47 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0.2718579234972651 1110 9 2.0 1110 10-11 0.42076502732240684 1110 12-13 1.4289617486338813 1110 14-15 1.5273224043715814 1110 16-17 2.9726775956284186 1110 18-19 2.446721311475411 1110 20-21 2.710382513661205 1110 22-23 3.6741803278688536 1110 24-25 3.1755464480874345 1110 26-27 0.8551912568305973 1110 28-29 3.020491803278688 1110 30-31 1.5901639344262293 1110 32-33 3.008879781420765 1110 34-35 2.349043715846994 1110 36-37 1.947404371584696 1110 38-39 -0.5922131147540988 1110 40-41 1.719945355191257 1110 42-43 0.17418032786885362 1110 44-45 2.64071038251366 1110 46-47 3.9187158469945373 1110 48-49 0.3107923497267713 1110 50-51 0.8572404371584685 1110 52-53 2.446721311475409 1110 54-55 4.8367486338797825 1110 56-57 3.318989071038253 1110 58-59 4.036885245901637 1110 60-61 0.9781420765027331 1110 62-63 -1.1523224043715867 1110 64-65 1.7356557377049153 1110 66-67 3.9836065573770494 1110 68-69 -7.407103825136613 1110 70-71 1.7520491803278677 1110 72-73 -6.762978142076504 1110 74-75 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2211 68-69 0.5928961748633874 2211 70-71 0.4187158469945338 2211 72-73 0.6536885245901622 2211 74-75 0.34562841530054556 2211 76-77 -0.1830601092896167 2211 78-79 0.29439890710382777 2211 80-81 0.5566939890710358 2211 82-83 1.2247267759562845 2211 84-85 1.3162568306010929 2211 86-87 1.5771857923497254 2211 88-89 1.5109289617486326 2211 90-91 1.415300546448087 2211 92-93 2.05191256830601 2211 94-95 2.556693989071036 2211 96-97 2.5122950819672134 2211 98-99 2.1673497267759547 2211 100 2.6311475409836067 2212 1 -14.438524590163933 2212 2 -14.173497267759561 2212 3 -14.25 2212 4 -16.329234972677593 2212 5 -15.975409836065573 2212 6 -16.737704918032787 2212 7 -16.77459016393443 2212 8 -16.228142076502735 2212 9 -16.5 2212 10-11 -17.079234972677593 2212 12-13 -17.07103825136612 2212 14-15 -16.97267759562842 2212 16-17 -16.27732240437158 2212 18-19 -15.803278688524589 2212 20-21 -15.539617486338795 2212 22-23 -15.325819672131146 2212 24-25 -15.074453551912566 2212 26-27 -15.394808743169403 2212 28-29 -14.229508196721312 2212 30-31 -14.40983606557377 2212 32-33 -13.741120218579235 2212 34-35 -13.900956284153006 2212 36-37 -14.052595628415304 2212 38-39 -14.592213114754099 2212 40-41 -14.280054644808743 2212 42-43 -14.325819672131146 2212 44-45 -14.35928961748634 2212 46-47 -14.581284153005463 2212 48-49 -14.189207650273229 2212 50-51 -13.392759562841531 2212 52-53 -13.55327868852459 2212 54-55 -12.913251366120218 2212 56-57 -12.681010928961747 2212 58-59 -12.713114754098363 2212 60-61 -12.271857923497267 2212 62-63 -11.152322404371587 2212 64-65 -11.264344262295085 2212 66-67 -11.51639344262295 2212 68-69 -11.407103825136613 2212 70-71 -11.247950819672132 2212 72-73 -11.012978142076504 2212 74-75 -11.32103825136612 2212 76-77 -11.183060109289617 2212 78-79 -10.705601092896172 2212 80-81 -9.776639344262296 2212 82-83 -9.775273224043715 2212 84-85 -9.683743169398907 2212 86-87 -9.422814207650275 2212 88-89 -9.489071038251367 2212 90-91 -9.584699453551913 2212 92-93 -8.94808743169399 2212 94-95 -8.276639344262296 2212 96-97 -8.154371584699453 2212 98-99 -8.165983606557377 2212 100 -8.368852459016393 2213 1 10.811475409836067 2213 2 11.326502732240439 2213 3 12.25 2213 4 12.920765027322407 2213 5 10.774590163934427 2213 6 7.762295081967213 2213 7 14.72540983606557 2213 8 15.771857923497265 2213 9 15.0 2213 10-11 9.420765027322407 2213 12-13 8.803961748633881 2213 14-15 10.527322404371581 2213 16-17 14.847677595628419 2213 18-19 2.571721311475411 2213 20-21 5.335382513661205 2213 22-23 5.174180327868854 2213 24-25 7.3005464480874345 2213 26-27 7.605191256830597 2213 28-29 6.145491803278688 2213 30-31 3.3401639344262293 2213 32-33 -0.49112021857923516 2213 34-35 -1.6509562841530059 2213 36-37 -1.052595628415304 2213 38-39 -1.5922131147540988 2213 40-41 1.469945355191257 2213 42-43 -3.4508196721311464 2213 44-45 -2.10928961748634 2213 46-47 2.5437158469945373 2213 48-49 2.1857923497267713 2213 50-51 1.7322404371584685 2213 52-53 0.19672131147540917 2213 54-55 2.7117486338797825 2213 56-57 2.943989071038253 2213 58-59 1.1618852459016367 2213 60-61 1.853142076502733 2213 62-63 5.597677595628413 2213 64-65 1.9856557377049153 2213 66-67 -2.5163934426229506 2213 68-69 -1.7821038251366126 2213 70-71 -2.6229508196721323 2213 72-73 -1.6379781420765038 2213 74-75 -1.5710382513661205 2213 76-77 -1.4330601092896167 2213 78-79 -1.0806010928961722 2213 80-81 -0.5266393442622963 2213 82-83 -0.27527322404371546 2213 84-85 -0.3087431693989071 2213 86-87 0.20218579234972545 2213 88-89 0.01092896174863256 2213 90-91 -0.334699453551913 2213 92-93 0.30191256830601 2213 94-95 0.2233606557377037 2213 96-97 0.5956284153005473 2213 98-99 0.5840163934426226 2213 100 0.3811475409836067 2214 1 -2.938524590163933 2214 2 -10.173497267759561 2214 3 -10.25 2214 4 -3.829234972677593 2214 5 -1.9754098360655732 2214 6 -4.237704918032787 2214 7 -3.7745901639344304 2214 8 -8.728142076502735 2214 9 -4.0 2214 10-11 -1.5792349726775932 2214 12-13 -1.5710382513661187 2214 14-15 -2.9726775956284186 2214 16-17 -12.277322404371581 2214 18-19 -7.553278688524589 2214 20-21 -1.7896174863387948 2214 22-23 -0.8258196721311464 2214 24-25 -3.5744535519125655 2214 26-27 -6.394808743169403 2214 28-29 -14.229508196721312 2214 30-31 -14.40983606557377 2214 32-33 -13.741120218579235 2214 34-35 -13.900956284153006 2214 36-37 -14.052595628415304 2214 38-39 -14.592213114754099 2214 40-41 -14.280054644808743 2214 42-43 -14.325819672131146 2214 44-45 -14.35928961748634 2214 46-47 -14.581284153005463 2214 48-49 -14.189207650273229 2214 50-51 -13.392759562841531 2214 52-53 -13.55327868852459 2214 54-55 -12.913251366120218 2214 56-57 -12.681010928961747 2214 58-59 -12.713114754098363 2214 60-61 -12.271857923497267 2214 62-63 -11.152322404371587 2214 64-65 -11.264344262295085 2214 66-67 -11.51639344262295 2214 68-69 -11.407103825136613 2214 70-71 -11.247950819672132 2214 72-73 -11.012978142076504 2214 74-75 -11.32103825136612 2214 76-77 -11.183060109289617 2214 78-79 -10.705601092896172 2214 80-81 -9.776639344262296 2214 82-83 -9.775273224043715 2214 84-85 -9.683743169398907 2214 86-87 -9.422814207650275 2214 88-89 -9.489071038251367 2214 90-91 -9.584699453551913 2214 92-93 -8.94808743169399 2214 94-95 -8.276639344262296 2214 96-97 -8.154371584699453 2214 98-99 -8.165983606557377 2214 100 -8.368852459016393 2215 1 -5.938524590163933 2215 2 -1.4234972677595614 2215 3 -4.25 2215 4 -2.579234972677593 2215 5 -3.475409836065573 2215 6 -1.7377049180327866 2215 7 -9.27459016393443 2215 8 -8.728142076502735 2215 9 -9.0 2215 10-11 -9.579234972677593 2215 12-13 -9.571038251366119 2215 14-15 -9.472677595628419 2215 16-17 -8.777322404371581 2215 18-19 -8.553278688524589 2215 20-21 -15.539617486338795 2215 22-23 -15.325819672131146 2215 24-25 -15.074453551912566 2215 26-27 -15.394808743169403 2215 28-29 -14.229508196721312 2215 30-31 -14.40983606557377 2215 32-33 -13.741120218579235 2215 34-35 -13.900956284153006 2215 36-37 -14.052595628415304 2215 38-39 -14.592213114754099 2215 40-41 -14.280054644808743 2215 42-43 -14.325819672131146 2215 44-45 -14.35928961748634 2215 46-47 -14.581284153005463 2215 48-49 -14.189207650273229 2215 50-51 -13.392759562841531 2215 52-53 -13.55327868852459 2215 54-55 -12.913251366120218 2215 56-57 -12.681010928961747 2215 58-59 -12.713114754098363 2215 60-61 -12.271857923497267 2215 62-63 -11.152322404371587 2215 64-65 -11.264344262295085 2215 66-67 -11.51639344262295 2215 68-69 -11.407103825136613 2215 70-71 -11.247950819672132 2215 72-73 -11.012978142076504 2215 74-75 -11.32103825136612 2215 76-77 -11.183060109289617 2215 78-79 -10.705601092896172 2215 80-81 -9.776639344262296 2215 82-83 -9.775273224043715 2215 84-85 -9.683743169398907 2215 86-87 -9.422814207650275 2215 88-89 -9.489071038251367 2215 90-91 -9.584699453551913 2215 92-93 -8.94808743169399 2215 94-95 -8.276639344262296 2215 96-97 -8.154371584699453 2215 98-99 -8.165983606557377 2215 100 -8.368852459016393 2216 1 -2.938524590163933 2216 2 -1.1734972677595614 2216 3 -0.25 2216 4 -2.329234972677593 2216 5 -0.9754098360655732 2216 6 -3.7377049180327866 2216 7 -9.27459016393443 2216 8 -8.728142076502735 2216 9 -1.5 2216 10-11 -1.8292349726775932 2216 12-13 -0.5710382513661187 2216 14-15 -6.972677595628419 2216 16-17 -2.0273224043715814 2216 18-19 -9.803278688524589 2216 20-21 -7.539617486338795 2216 22-23 -3.3258196721311464 2216 24-25 -0.5744535519125655 2216 26-27 -1.1448087431694027 2216 28-29 -0.9795081967213122 2216 30-31 -0.6598360655737707 2216 32-33 -2.741120218579235 2216 34-35 1.0990437158469941 2216 36-37 3.197404371584696 2216 38-39 1.1577868852459012 2216 40-41 -2.030054644808743 2216 42-43 -1.5758196721311464 2216 44-45 2.39071038251366 2216 46-47 2.1687158469945373 2216 48-49 2.3107923497267713 2216 50-51 3.3572404371584685 2216 52-53 -6.553278688524591 2216 54-55 -0.4132513661202175 2216 56-57 1.3189890710382528 2216 58-59 3.7868852459016367 2216 60-61 -0.2718579234972669 2216 62-63 -0.40232240437158673 2216 64-65 4.485655737704915 2216 66-67 2.7336065573770494 2216 68-69 -2.1571038251366126 2216 70-71 -1.9979508196721323 2216 72-73 0.7370218579234962 2216 74-75 4.6789617486338795 2216 76-77 -4.683060109289617 2216 78-79 0.7943989071038278 2216 80-81 5.723360655737704 2216 82-83 4.2247267759562845 2216 84-85 6.316256830601093 2216 86-87 5.3271857923497254 2216 88-89 5.760928961748633 2216 90-91 5.165300546448087 2216 92-93 2.05191256830601 2216 94-95 1.4733606557377037 2216 96-97 4.595628415300547 2216 98-99 6.084016393442623 2216 100 6.131147540983607 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 498.0 3 29.0 4 24.0 5 28.0 6 28.0 7 27.0 8 27.0 9 16.0 10 22.0 11 13.0 12 15.0 13 15.0 14 18.0 15 12.0 16 13.0 17 9.0 18 8.0 19 10.0 20 11.0 21 11.0 22 12.0 23 12.0 24 14.0 25 14.0 26 17.0 27 21.0 28 24.0 29 20.0 30 26.0 31 21.0 32 22.0 33 30.0 34 40.0 35 49.0 36 53.0 37 47.0 38 45.0 39 3.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.944785276073624 21.319018404907975 12.116564417177914 23.619631901840492 2 19.478527607361965 21.088957055214724 33.12883435582822 26.30368098159509 3 21.932515337423315 27.837423312883438 24.309815950920246 25.920245398773005 4 14.570552147239264 18.865030674846626 32.74539877300614 33.81901840490797 5 18.021472392638035 37.26993865030675 29.06441717791411 15.644171779141105 6 32.668711656441715 34.66257668711656 16.027607361963188 16.641104294478527 7 28.834355828220858 31.441717791411044 18.865030674846626 20.858895705521473 8 26.07361963190184 35.35276073619632 18.25153374233129 20.322085889570552 9 27.76073619631902 17.791411042944784 19.24846625766871 35.19938650306749 10-11 25.72852760736196 26.11196319018405 25.4601226993865 22.699386503067483 12-13 24.578220858895705 25.191717791411044 25.191717791411044 25.03834355828221 14-15 24.041411042944784 26.265337423312886 23.466257668711656 26.226993865030675 16-17 27.14723926380368 27.952453987730063 22.085889570552148 22.81441717791411 18-19 25.07668711656442 27.99079754601227 24.118098159509202 22.81441717791411 20-21 24.616564417177912 27.37730061349693 25.4601226993865 22.54601226993865 22-23 26.533742331288344 27.799079754601223 22.7760736196319 22.891104294478527 24-25 24.003067484662576 26.035276073619634 24.233128834355828 25.72852760736196 26-27 25.920245398773005 25.345092024539877 23.044478527607364 25.690184049079758 28-29 26.380368098159508 26.07361963190184 22.737730061349694 24.80828220858896 30-31 24.118098159509202 26.878834355828218 23.236196319018404 25.766871165644172 32-33 24.003067484662576 26.035276073619634 21.472392638036812 28.48926380368098 34-35 27.837423312883438 22.354294478527606 23.96472392638037 25.84355828220859 36-37 26.725460122699385 22.124233128834355 22.929447852760738 28.22085889570552 38-39 21.779141104294478 24.654907975460123 24.961656441717793 28.60429447852761 40-41 24.079754601226995 24.118098159509202 22.430981595092025 29.371165644171782 42-43 23.88803680981595 24.309815950920246 25.11503067484663 26.68711656441718 44-45 25.153374233128833 24.42484662576687 25.4601226993865 24.961656441717793 46-47 23.6579754601227 24.961656441717793 25.4601226993865 25.920245398773005 48-49 24.884969325153374 23.044478527607364 23.734662576687114 28.335889570552148 50-51 24.42484662576687 24.654907975460123 24.003067484662576 26.91717791411043 52-53 27.032208588957058 23.734662576687114 24.15644171779141 25.07668711656442 54-55 23.466257668711656 26.95552147239264 22.469325153374236 27.108895705521473 56-57 25.07668711656442 24.348159509202453 23.58128834355828 26.993865030674847 58-59 26.533742331288344 24.233128834355828 23.044478527607364 26.188650306748468 60-61 26.41871165644172 23.773006134969325 23.12116564417178 26.68711656441718 62-63 26.150306748466257 25.57515337423313 23.811349693251536 24.46319018404908 64-65 24.731595092024538 25.920245398773005 23.849693251533743 25.49846625766871 66-67 26.993865030674847 23.734662576687114 22.7760736196319 26.495398773006134 68-69 27.300613496932513 22.891104294478527 22.354294478527606 27.453987730061353 70-71 26.226993865030675 23.044478527607364 23.31288343558282 27.415644171779142 72-73 26.95552147239264 24.15644171779141 23.044478527607364 25.84355828220859 74-75 30.214723926380366 23.044478527607364 22.04754601226994 24.69325153374233 76-77 28.412576687116562 22.85276073619632 22.737730061349694 25.996932515337424 78-79 28.796012269938647 23.427914110429448 23.466257668711656 24.309815950920246 80-81 27.952453987730063 23.619631901840492 22.23926380368098 26.188650306748468 82-83 29.026073619631905 23.734662576687114 22.469325153374236 24.76993865030675 84-85 26.533742331288344 25.153374233128833 21.855828220858893 26.45705521472393 86-87 27.72239263803681 23.734662576687114 22.085889570552148 26.45705521472393 88-89 28.642638036809814 23.31288343558282 22.00920245398773 26.035276073619634 90-91 26.188650306748468 22.277607361963188 24.76993865030675 26.763803680981596 92-93 27.914110429447852 23.159509202453986 23.92638036809816 25.0 94-95 25.61349693251534 23.811349693251536 24.42484662576687 26.150306748466257 96-97 26.91717791411043 22.584355828220858 23.504601226993866 26.993865030674847 98-99 26.840490797546014 23.27453987730061 25.345092024539877 24.539877300613497 100 27.070552147239262 23.006134969325153 22.3159509202454 27.607361963190186 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.5 16 1.0 17 0.5 18 0.0 19 0.0 20 2.0 21 2.5 22 1.0 23 2.5 24 2.5 25 2.5 26 2.5 27 4.5 28 6.0 29 7.5 30 12.0 31 15.5 32 14.0 33 11.5 34 17.0 35 20.0 36 24.5 37 30.0 38 28.5 39 27.0 40 31.5 41 32.5 42 27.0 43 29.0 44 30.5 45 32.5 46 38.0 47 38.0 48 35.0 49 32.5 50 36.5 51 41.0 52 37.5 53 36.0 54 36.0 55 38.0 56 40.0 57 35.0 58 32.5 59 29.5 60 24.0 61 30.0 62 35.0 63 26.0 64 22.0 65 23.0 66 24.5 67 24.0 68 17.5 69 16.5 70 17.0 71 13.0 72 11.5 73 10.5 74 8.0 75 7.0 76 7.5 77 7.5 78 6.0 79 5.0 80 6.5 81 8.0 82 9.0 83 5.0 84 0.0 85 2.0 86 2.5 87 1.0 88 1.0 89 1.5 90 2.5 91 1.5 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1304.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.76993865030674 #Duplication Level Percentage of deduplicated Percentage of total 1 99.76940814757879 99.5398773006135 2 0.23059185242121444 0.4601226993865031 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.15337423312883436 No Hit ACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACT 2 0.15337423312883436 No Hit GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC 2 0.15337423312883436 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.03834355828220859 0.0 34-35 0.0 0.0 0.0 0.15337423312883436 0.0 36-37 0.0 0.0 0.0 0.15337423312883436 0.0 38-39 0.0 0.0 0.0 0.15337423312883436 0.0 40-41 0.0 0.0 0.0 0.23006134969325154 0.0 42-43 0.0 0.0 0.0 0.2684049079754601 0.0 44-45 0.0 0.0 0.0 0.3450920245398773 0.0 46-47 0.0 0.0 0.0 0.3834355828220859 0.0 48-49 0.0 0.0 0.0 0.4601226993865031 0.0 50-51 0.0 0.0 0.0 0.5751533742331288 0.0 52-53 0.0 0.0 0.0 0.6901840490797546 0.0 54-55 0.0 0.0 0.0 0.7285276073619632 0.0 56-57 0.0 0.0 0.0 0.8052147239263804 0.0 58-59 0.0 0.0 0.0 0.8819018404907976 0.0 60-61 0.0 0.0 0.0 0.9969325153374233 0.0 62-63 0.0 0.0 0.0 0.9969325153374233 0.0 64-65 0.0 0.0 0.0 0.9969325153374233 0.0 66-67 0.0 0.0 0.0 0.9969325153374233 0.0 68-69 0.0 0.0 0.0 0.9969325153374233 0.0 70-71 0.0 0.0 0.0 1.1503067484662577 0.0 72-73 0.0 0.0 0.0 1.3803680981595092 0.0 74-75 0.0 0.0 0.0 1.7638036809815951 0.0 76-77 0.0 0.0 0.0 2.0322085889570554 0.0 78-79 0.0 0.0 0.0 2.147239263803681 0.0 80-81 0.0 0.0 0.0 2.2622699386503067 0.0 82-83 0.0 0.0 0.0 2.415644171779141 0.0 84-85 0.0 0.0 0.0 2.5690184049079754 0.0 86-87 0.0 0.0 0.0 2.684049079754601 0.0 88 0.0 0.0 0.0 2.684049079754601 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE