FastQCFastQC Report
Wed 25 May 2016
SRR1032991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACT100.7668711656441718No Hit
CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCC80.6134969325153374No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG80.6134969325153374TruSeq Adapter, Index 1 (96% over 28bp)
CTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTG80.6134969325153374No Hit
GTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCG50.3834355828220859No Hit
CCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTC50.3834355828220859No Hit
GTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGC50.3834355828220859No Hit
GTCTTCATGCAGGAACCACAGTGCCAGATCCCCACAGCTCGTCTCTTCAT40.3067484662576687No Hit
ATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAA40.3067484662576687No Hit
CAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCA40.3067484662576687No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG30.23006134969325154RNA PCR Primer, Index 39 (96% over 30bp)
GGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCC30.23006134969325154No Hit
GAGTGCCCAGGTTCACAGTGCAGCTTGTGGCTCGTGTCCATCTTGCAGGT30.23006134969325154No Hit
GTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTT30.23006134969325154No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG30.23006134969325154TruSeq Adapter, Index 3 (96% over 28bp)
ACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGG30.23006134969325154No Hit
CTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGG30.23006134969325154No Hit
GCTCAATGGTCTCTTTGGTCGGCAGGGTGTTCTTCTCCTGCGTCTCCGTT30.23006134969325154No Hit
CCATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGC30.23006134969325154No Hit
GTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTA30.23006134969325154No Hit
CCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTC30.23006134969325154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.23006134969325154No Hit
CGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGC30.23006134969325154No Hit
TTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTAC30.23006134969325154No Hit
TTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTA30.23006134969325154No Hit
CTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCT30.23006134969325154No Hit
GGGGTAGGAAATCCTCCAGGATCTTAGGAAATTTCACTCCGCTTCTCCTG30.23006134969325154No Hit
GTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCC30.23006134969325154No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT30.23006134969325154TruSeq Adapter, Index 1 (96% over 26bp)
CGCTATGACTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGA20.15337423312883436No Hit
GAGTACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCAT20.15337423312883436No Hit
GTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATC20.15337423312883436No Hit
GTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTG20.15337423312883436No Hit
GATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGA20.15337423312883436No Hit
GAGCACGCCATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGG20.15337423312883436No Hit
TTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGA20.15337423312883436No Hit
GGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGC20.15337423312883436No Hit
GTATCAACGCAGAGTACATGGGGCTTCCTTTTGCGGGTGGCGGCGAACGC20.15337423312883436No Hit
CGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACAC20.15337423312883436No Hit
GTACCTGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGA20.15337423312883436No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.15337423312883436No Hit
GTCTCAAAGAGTAGAGGAGCGTCTACTGGTCTTTCAACTCCTTCAGTCTT20.15337423312883436No Hit
CTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCA20.15337423312883436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.15337423312883436No Hit
GTAGAGGAGCGTCTACTGGTCTTTCAACTCCTTCAGTCTTCTGATGGCGG20.15337423312883436No Hit
TCTTGCAGGTGGCTCTTCCTCCACATCACGACTGGGGTCTCGAAGAGGAC20.15337423312883436No Hit
GGCCAGTGATGTCTCAAAGAGTAGAGGAGCGTCTACTGGTCTTTCAACTC20.15337423312883436No Hit
ACCGGGACCTGACCACCGCAGGCGCTGTCACCCAGTGCTACCGAGACATG20.15337423312883436No Hit
CTCTTCCTCCACATCACGACTGGGGTCTCGAAGAGGACGGGGGTAGGAAA20.15337423312883436No Hit
CCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAA20.15337423312883436No Hit
GGTTCATCTTGCTAGTGATAGTGGAGAAAATCAATCTCTTCTGGCGGCGG20.15337423312883436No Hit
GTACATGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAA20.15337423312883436No Hit
CCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTCACCCAGTGCTACC20.15337423312883436No Hit
GTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTG20.15337423312883436No Hit
AGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTC20.15337423312883436No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA20.15337423312883436RNA PCR Primer, Index 1 (96% over 28bp)
GCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTC20.15337423312883436No Hit
GTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCC20.15337423312883436No Hit
CCCTTCAGAGACCCACAGGCCGGTGGCATCGGCGCGGAGTGCCCAGGTTC20.15337423312883436No Hit
ACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTCACCCAG20.15337423312883436No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG20.15337423312883436RNA PCR Primer, Index 23 (96% over 30bp)
GGCTATATTAGTGCTGCAGAACTTCGCCATGTGATGACAAACCTTGGAGA20.15337423312883436No Hit
AAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCA20.15337423312883436No Hit
TACCGGGACCTGACCACCGCAGGCGCTGTCACCCAGTGCTACCGAGACAT20.15337423312883436No Hit
GAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACT20.15337423312883436No Hit
GCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGT20.15337423312883436No Hit
GACTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACCTGAC20.15337423312883436No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)