FastQCFastQC Report
Wed 25 May 2016
SRR1032990_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032990_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179138
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47550.032.124611
GGTATCA32450.031.4304121
CCGCGTA902.5953388E-531.3333599
TCAACGC48100.030.1938464
ATCAACG48900.029.6998773
CAACGCA49800.029.1631365
GTACATG105800.027.9428061
AACGCAG52850.027.6575456
TACATGG104350.026.6645532
TATCAAC57850.025.836212
ACATGGG108450.024.7030353
ACGCAGA64550.022.2076117
CGCAGAG65850.021.5550678
CATGGGG64400.021.3838944
GAGTACA74500.018.0432531
GCAGAGT78150.017.6813969
GTATAGG13050.017.6478021
CATGGGA37800.016.9103344
AGTACAT75450.016.6947772
GTACCGA1700.001919630616.5882476
Produced by FastQC (version 0.11.5)