FastQCFastQC Report
Wed 25 May 2016
SRR1032990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032990_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179138
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59610.18750365665158292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46870.14742990080959054No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49400.047.8366281
GTATCAA90000.043.2222481
TCAACGC106350.036.0025144
ATCAACG106500.035.9082033
CAACGCA108700.035.177025
AACGCAG113400.033.8020136
TATCAAC115900.033.2399252
ACGCAGA130650.029.1586077
CGCAGAG132750.028.51938
GTACATG101900.027.3400971
TACATGG100850.026.7729112
GCAGAGT146350.025.6760379
ACATGGG107450.024.6026943
TAGGTCG2355.2204996E-922.0152135
GAGTACT94050.021.25061612-13
GTGGTAT26650.020.9782521
AGAGTAC134650.020.7733310-11
CATGGGG68100.020.445164
CAGAGTA146950.020.10535610-11
GAGTACA76450.020.0336321
Produced by FastQC (version 0.11.5)