FastQCFastQC Report
Wed 25 May 2016
SRR1032989_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032989_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799146
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.036.185131
GTATCAA40500.034.8158071
TCAACGC43900.030.6204624
ATCAACG44100.030.5881773
CAACGCA45700.029.5172545
AACGCAG46650.029.0169056
GTACATG101900.027.6749821
TACATGG101800.026.2247162
TATCAAC52700.026.1316782
CGCGTAT951.3358658E-824.73577912-13
ACATGGG103750.024.7351633
ACGCAGA58600.023.0996347
CGCAGAG61750.021.616818
GAGTACA74650.021.1553251
CATGGGG71800.021.0131344
AGTACAT75250.019.862362
TCCGTAC1708.631154E-519.3534933
TATACTG16550.018.1757455
GCAGAGT71550.017.9334269
CGCGAAC850.001920438616.58752328-29
Produced by FastQC (version 0.11.5)