##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032989_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2799146
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73849917081853	33.0	31.0	34.0	30.0	34.0
2	31.753772757833996	34.0	31.0	34.0	30.0	34.0
3	31.91925537288873	34.0	31.0	34.0	30.0	34.0
4	35.21909682453148	37.0	35.0	37.0	33.0	37.0
5	35.31029714062789	37.0	35.0	37.0	33.0	37.0
6	35.364353985108316	37.0	37.0	37.0	35.0	37.0
7	35.32074211205846	37.0	37.0	37.0	35.0	37.0
8	35.33012032955766	37.0	37.0	37.0	35.0	37.0
9	36.98996015213211	39.0	38.0	39.0	35.0	39.0
10-11	36.98500221138876	39.0	38.0	39.0	35.0	39.0
12-13	36.93183617431888	39.0	38.0	39.0	35.0	39.0
14-15	38.427368383071126	41.0	39.0	41.0	35.0	41.0
16-17	38.450915386335694	41.0	39.0	41.0	35.0	41.0
18-19	38.45625647965487	41.0	39.0	41.0	35.0	41.0
20-21	38.503814020419085	41.0	39.0	41.0	35.0	41.0
22-23	38.49313754980983	41.0	39.0	41.0	34.0	41.0
24-25	38.52352092388178	41.0	39.0	41.0	34.0	41.0
26-27	38.501700340032286	41.0	39.0	41.0	34.0	41.0
28-29	38.478540776365364	41.0	39.0	41.0	34.0	41.0
30-31	38.42804519664212	41.0	39.0	41.0	34.0	41.0
32-33	38.38128200529733	41.0	39.0	41.0	34.0	41.0
34-35	38.307864612992674	41.0	39.0	41.0	34.0	41.0
36-37	38.21295709477104	41.0	39.0	41.0	33.5	41.0
38-39	38.118795875599204	41.0	39.0	41.0	33.0	41.0
40-41	38.028917748484716	41.0	38.0	41.0	33.0	41.0
42-43	37.908089645913435	40.5	38.0	41.0	33.0	41.0
44-45	37.76264296324665	40.0	38.0	41.0	33.0	41.0
46-47	37.63064716881506	40.0	38.0	41.0	33.0	41.0
48-49	37.49449385634047	40.0	38.0	41.0	32.0	41.0
50-51	37.341758700689425	40.0	37.0	41.0	32.0	41.0
52-53	37.180310887677884	40.0	37.0	41.0	31.5	41.0
54-55	37.00483879726174	40.0	36.5	41.0	31.0	41.0
56-57	36.811504651775934	40.0	36.0	41.0	31.0	41.0
58-59	36.59217275554759	39.5	35.0	41.0	31.0	41.0
60-61	36.337053336982066	39.0	35.0	41.0	30.5	41.0
62-63	36.073619060956446	39.0	35.0	41.0	30.0	41.0
64-65	35.78850442242027	38.0	35.0	40.0	30.0	41.0
66-67	35.48628117290059	37.5	35.0	40.0	30.0	41.0
68-69	35.16878272873226	37.0	35.0	40.0	29.5	41.0
70-71	34.82944083659802	36.5	34.0	39.0	29.0	41.0
72-73	34.53799337369326	36.0	34.0	39.0	29.0	41.0
74-75	34.26092011634977	35.5	34.0	39.0	29.0	40.5
76-77	33.94045630345827	35.0	34.0	37.5	29.0	40.0
78-79	33.58459955286362	35.0	34.0	37.0	29.0	39.0
80-81	33.27128077635107	35.0	34.0	37.0	28.5	39.0
82-83	32.966267032873596	35.0	34.0	36.0	28.0	38.5
84-85	32.81164969601443	35.0	34.0	36.0	28.0	37.0
86-87	32.58994171793826	35.0	34.0	36.0	28.0	37.0
88-89	32.36882124047834	35.0	34.0	35.0	27.0	36.5
90-91	32.179380246689526	35.0	34.0	35.0	27.0	36.0
92-93	31.99693120687524	35.0	33.5	35.0	27.0	36.0
94-95	31.821181353169862	35.0	33.0	35.0	26.0	36.0
96-97	31.65135544912627	35.0	33.0	35.0	25.5	35.5
98-99	31.47344565092353	35.0	33.0	35.0	25.0	35.0
100	31.34630633771872	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.339727278864693
1101	2	0.38178903512222817
1101	3	0.4198434730943923
1101	4	0.395439366723771
1101	5	0.3117529081732542
1101	6	0.32969175788979754
1101	7	0.389150710079214
1101	8	0.3318041700643519
1101	9	0.41657342809638465
1101	10-11	0.4280902213019786
1101	12-13	0.24885753505569852
1101	14-15	0.3144639991431859
1101	16-17	0.27719365586855815
1101	18-19	0.2999958192989638
1101	20-21	0.291617012915097
1101	22-23	0.32249820809413166
1101	24-25	0.3343426785518986
1101	26-27	0.3859880064024779
1101	28-29	0.33890862135273636
1101	30-31	0.31563449970422397
1101	32-33	0.30007432251207433
1101	34-35	0.3020940574388007
1101	36-37	0.28419990885330293
1101	38-39	0.3100238447582271
1101	40-41	0.33246859351075386
1101	42-43	0.30111625411751675
1101	44-45	0.17327080162259278
1101	46-47	0.14471044622250417
1101	48-49	0.2684538600902542
1101	50-51	0.27947424221822104
1101	52-53	0.23996668723332704
1101	54-55	0.22298633385025823
1101	56-57	0.2624401450791609
1101	58-59	0.2653965905614655
1101	60-61	0.3138495149342617
1101	62-63	0.3117822381029569
1101	64-65	0.18571400899084267
1101	66-67	0.28992528581608923
1101	68-69	0.32285699903225407
1101	70-71	0.2536249344929118
1101	72-73	0.1333939834121125
1101	74-75	0.1308161960616303
1101	76-77	0.12908932815403773
1101	78-79	0.17717010651657006
1101	80-81	0.242398830636958
1101	82-83	0.19942387334706524
1101	84-85	0.1971516492640788
1101	86-87	0.16410753057630245
1101	88-89	0.16300054215179216
1101	90-91	0.19372091114900059
1101	92-93	0.241281301583669
1101	94-95	0.24792886395590585
1101	96-97	0.19900879325004794
1101	98-99	0.1620064047655596
1101	100	0.24721125521076104
1102	1	0.12841426769064768
1102	2	0.13664045950741865
1102	3	0.1698538927301918
1102	4	0.1649585613712361
1102	5	0.1307760022820048
1102	6	0.1138971844361194
1102	7	0.1526681945078039
1102	8	0.14242330342478482
1102	9	0.1947791024761898
1102	10-11	0.19200581973452557
1102	12-13	0.21030586163421816
1102	14-15	0.25664851704692637
1102	16-17	0.21195568677165966
1102	18-19	0.2086798794380158
1102	20-21	0.18669575599093235
1102	22-23	0.18160808867811795
1102	24-25	0.25738164015756837
1102	26-27	0.2696663392383698
1102	28-29	0.28026747053263534
1102	30-31	0.25688712454334706
1102	32-33	0.2999143915921678
1102	34-35	0.3179150506133084
1102	36-37	0.30912458677467214
1102	38-39	0.3738299880636049
1102	40-41	0.3643388328211117
1102	42-43	0.323047139904034
1102	44-45	0.28091347161160485
1102	46-47	0.2894571595616853
1102	48-49	0.26077640669958413
1102	50-51	0.2771973070400975
1102	52-53	0.23384092041106896
1102	54-55	0.2293465075127159
1102	56-57	0.2160152829610169
1102	58-59	0.05704394477117347
1102	60-61	0.1980652632137634
1102	62-63	0.26216798840484756
1102	64-65	0.2387736616155749
1102	66-67	0.23318484439835885
1102	68-69	0.23266582039382655
1102	70-71	0.22788423824864878
1102	72-73	0.2041224711998808
1102	74-75	0.21061207469706744
1102	76-77	0.1998959981755064
1102	78-79	0.1614279948801709
1102	80-81	0.12705311271566444
1102	82-83	0.16769391450252868
1102	84-85	0.11505037376079486
1102	86-87	0.15420069470779652
1102	88-89	0.0909457536088567
1102	90-91	0.09180246738664266
1102	92-93	0.10067111372533333
1102	94-95	0.0831390371008851
1102	96-97	0.15817626854571998
1102	98-99	0.14843262633148768
1102	100	0.14543518982747727
1103	1	0.10386237452956237
1103	2	0.18023373829879574
1103	3	0.24534168943261747
1103	4	0.2001570066824243
1103	5	0.13566445266526728
1103	6	0.08232010530559108
1103	7	0.1358336561001181
1103	8	0.14076212505249686
1103	9	0.17901667114887232
1103	10-11	0.20296113017683837
1103	12-13	0.15141368959174173
1103	14-15	0.2515292324473677
1103	16-17	0.25169042173231304
1103	18-19	0.2489886617685002
1103	20-21	0.23240093081730606
1103	22-23	0.23051776592687645
1103	24-25	0.21499848821082423
1103	26-27	0.3277791369757779
1103	28-29	0.3094048650346508
1103	30-31	0.2559446906038616
1103	32-33	0.3320733305320118
1103	34-35	0.354826289815783
1103	36-37	0.31769116037759915
1103	38-39	0.2630365086327586
1103	40-41	0.352039315376139
1103	42-43	0.28159660626768357
1103	44-45	0.26364003435992345
1103	46-47	0.23672466203027653
1103	48-49	0.2826161814449222
1103	50-51	0.3305268218488777
1103	52-53	0.3212943028501485
1103	54-55	0.3182075980651575
1103	56-57	0.2840834656593856
1103	58-59	0.23629963429708312
1103	60-61	0.30922238833218785
1103	62-63	0.2819619055993954
1103	64-65	0.2747479422635024
1103	66-67	0.2264837077351487
1103	68-69	0.26605318197676553
1103	70-71	0.29983313340945017
1103	72-73	0.30320336774207846
1103	74-75	0.3499827232684325
1103	76-77	0.3030223361392359
1103	78-79	0.2670095755783919
1103	80-81	0.20396918361148408
1103	82-83	0.25122919160642
1103	84-85	0.23281047925143383
1103	86-87	0.2070292785675889
1103	88-89	0.22662532500106636
1103	90-91	0.16697976969921058
1103	92-93	0.21101047007112328
1103	94-95	0.16820087823512608
1103	96-97	0.10055098038689891
1103	98-99	0.1301813654571724
1103	100	0.19089998190040447
1104	1	-0.06047294935560643
1104	2	0.026821539204853195
1104	3	0.05460821907975699
1104	4	0.029626220442530382
1104	5	0.05303592868816054
1104	6	0.03098678886495776
1104	7	0.026946157500518098
1104	8	0.036852335162109284
1104	9	0.12683346749273738
1104	10-11	0.09908716224475
1104	12-13	0.10586880287723943
1104	14-15	0.13438872766516852
1104	16-17	0.11489520589955049
1104	18-19	0.11019330084715762
1104	20-21	0.1283582716883842
1104	22-23	0.10273958655443494
1104	24-25	0.13116266133906151
1104	26-27	0.1509961649211533
1104	28-29	0.13814628850538213
1104	30-31	0.14601517175385936
1104	32-33	0.22641434219814016
1104	34-35	0.12406885540887203
1104	36-37	0.14781356214407992
1104	38-39	0.20501159249922551
1104	40-41	0.19583438624895422
1104	42-43	0.1711588915208324
1104	44-45	0.12092760339436381
1104	46-47	0.06256518215739248
1104	48-49	0.13042607707761533
1104	50-51	0.17187477705817855
1104	52-53	0.11065086587666428
1104	54-55	0.11042544805052046
1104	56-57	0.08783232041786704
1104	58-59	0.12349268609769126
1104	60-61	0.1572686960272378
1104	62-63	0.15156039482599226
1104	64-65	0.11089959121191839
1104	66-67	0.15741266951189914
1104	68-69	0.09129337881874022
1104	70-71	0.08092048355050707
1104	72-73	0.11146340969171575
1104	74-75	0.12585093627750155
1104	76-77	0.17312599858396993
1104	78-79	0.08696375048293703
1104	80-81	0.13169700001166262
1104	82-83	0.13833288144819988
1104	84-85	0.1594570770464685
1104	86-87	0.1705250309322608
1104	88-89	0.15913196428269316
1104	90-91	0.1860574063657623
1104	92-93	0.15433929596881768
1104	94-95	0.18413528276292368
1104	96-97	0.24411288053791225
1104	98-99	0.1824790128741789
1104	100	0.06191353494860863
1105	1	0.05140017352339399
1105	2	0.024812565401493458
1105	3	0.09467510417168157
1105	4	0.06509744208425872
1105	5	0.06831328262854441
1105	6	0.09545941996288576
1105	7	0.09574986634482485
1105	8	0.0708621592959986
1105	9	0.10526503876804583
1105	10-11	0.08008840790980543
1105	12-13	0.08686374888267068
1105	14-15	0.13721934383795542
1105	16-17	0.13568893089507128
1105	18-19	0.12920626029692528
1105	20-21	0.09424447229017119
1105	22-23	0.1213915199230513
1105	24-25	0.15547860631487254
1105	26-27	0.13571198634951287
1105	28-29	0.18515181633316757
1105	30-31	0.1782691819349722
1105	32-33	0.15960957093476225
1105	34-35	0.15339732219339197
1105	36-37	0.1764616039288569
1105	38-39	0.12900175243090928
1105	40-41	0.12407802076715768
1105	42-43	0.1373240682253538
1105	44-45	0.17664556046909752
1105	46-47	0.09839749164164147
1105	48-49	0.12714049578177367
1105	50-51	0.09922033951310283
1105	52-53	0.11797777145730493
1105	54-55	0.12115472567534624
1105	56-57	0.11358004832519697
1105	58-59	0.13088907321819931
1105	60-61	0.09720928529616657
1105	62-63	0.14520128989398273
1105	64-65	0.2105204011122197
1105	66-67	0.11375111835412355
1105	68-69	0.14351091767637314
1105	70-71	0.10764712915284491
1105	72-73	0.10465946850113284
1105	74-75	0.09656829642861453
1105	76-77	0.08834082531461007
1105	78-79	0.14040271305005092
1105	80-81	0.09780896929699168
1105	82-83	0.06561322389769231
1105	84-85	0.02557007157052027
1105	86-87	-0.058622647973592734
1105	88-89	-0.06353355016387496
1105	90-91	-0.09327160083073238
1105	92-93	-0.11925106100633442
1105	94-95	-0.11460970769525858
1105	96-97	-0.07638965195613423
1105	98-99	0.011947474153291893
1105	100	0.018706763629772638
1106	1	0.05577679482971476
1106	2	0.07660016883902543
1106	3	0.040018060275041734
1106	4	0.1167980927806056
1106	5	0.09645698652547452
1106	6	0.1135310980058648
1106	7	0.07287404935136266
1106	8	0.09464373401014115
1106	9	0.05119492587440533
1106	10-11	0.08892498023274698
1106	12-13	0.07853802391419151
1106	14-15	0.11984328772445707
1106	16-17	0.09311831802316561
1106	18-19	0.060288966845917
1106	20-21	0.09930584643332452
1106	22-23	0.16308898235222102
1106	24-25	0.12777328915474584
1106	26-27	0.17482985079019642
1106	28-29	0.1239443326005727
1106	30-31	0.12634028029301447
1106	32-33	0.1482477460865681
1106	34-35	0.16753076566686786
1106	36-37	0.1605347601684386
1106	38-39	0.16630447839934703
1106	40-41	0.17390194146626214
1106	42-43	0.18589804326991555
1106	44-45	0.1493170385207776
1106	46-47	0.11654163030227238
1106	48-49	0.19779684405648368
1106	50-51	0.07792778701538339
1106	52-53	0.08895004802499074
1106	54-55	0.1076915429151768
1106	56-57	0.11409089567965935
1106	58-59	0.1560872530074846
1106	60-61	0.08352147462127135
1106	62-63	0.06105639764292192
1106	64-65	0.17089531234795174
1106	66-67	0.08010040751546654
1106	68-69	0.12078726797998485
1106	70-71	0.04660268180997207
1106	72-73	0.05424499376815817
1106	74-75	0.0795943967430901
1106	76-77	0.09843821238473538
1106	78-79	0.13195380503155718
1106	80-81	0.03563413631871981
1106	82-83	0.10457892104130195
1106	84-85	0.048898767424326195
1106	86-87	0.04586556268391462
1106	88-89	0.04283501352175989
1106	90-91	0.023422600870645738
1106	92-93	-0.026280465524983754
1106	94-95	-0.019639199166640253
1106	96-97	0.05449233868195691
1106	98-99	0.059843942422784124
1106	100	0.016119919579512754
1107	1	0.037786046827225306
1107	2	0.005016889286526549
1107	3	0.09208282086986586
1107	4	0.14890607001705547
1107	5	0.1021863816901103
1107	6	0.036184376208900915
1107	7	0.08989595184345944
1107	8	0.07304489895101796
1107	9	0.10049385528289179
1107	10-11	0.09323955831150244
1107	12-13	0.038262756379239704
1107	14-15	0.08063736951762479
1107	16-17	0.12517965697116296
1107	18-19	0.07318100937145289
1107	20-21	0.058686805025189415
1107	22-23	0.0678744038782213
1107	24-25	0.05471743105258042
1107	26-27	0.08970263723595195
1107	28-29	0.07383081410307568
1107	30-31	0.11345346057823491
1107	32-33	0.0659741351707197
1107	34-35	0.0872157087833969
1107	36-37	0.10680654846449045
1107	38-39	0.03400842351296518
1107	40-41	0.10411660745763385
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2216	34-35	-0.28022337409879583
2216	36-37	-0.317413888803344
2216	38-39	-0.2777563776503911
2216	40-41	-0.21220593156099454
2216	42-43	-0.20677815830828195
2216	44-45	-0.198567122765688
2216	46-47	-0.18339335893732311
2216	48-49	-0.13212902873165433
2216	50-51	-0.1449563742710822
2216	52-53	-0.21992392076984402
2216	54-55	-0.1748175735362807
2216	56-57	-0.16424296072874967
2216	58-59	-0.13305370825506913
2216	60-61	-0.14561530204061768
2216	62-63	-0.1071757580242263
2216	64-65	-0.1770336839456803
2216	66-67	-0.2188893103228864
2216	68-69	-0.14281268419600934
2216	70-71	-0.18000838944779218
2216	72-73	-0.20607266225766807
2216	74-75	-0.23019495344820484
2216	76-77	-0.258833278528698
2216	78-79	-0.14206468193640376
2216	80-81	-0.18880430521643632
2216	82-83	-0.19987319517104396
2216	84-85	-0.1746803364733296
2216	86-87	-0.18545692057768548
2216	88-89	-0.20402093160019064
2216	90-91	-0.23818779786201816
2216	92-93	-0.2513148390304778
2216	94-95	-0.1958893486166673
2216	96-97	-0.23885750887901835
2216	98-99	-0.2481015680984946
2216	100	-0.29904776334731764
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36912.0
3	5181.0
4	3491.0
5	3051.0
6	2552.0
7	2973.0
8	3454.0
9	3716.0
10	3873.0
11	3990.0
12	4463.0
13	4720.0
14	5056.0
15	5252.0
16	5325.0
17	5217.0
18	5093.0
19	4972.0
20	5072.0
21	5442.0
22	6522.0
23	8736.0
24	13551.0
25	18665.0
26	20252.0
27	17812.0
28	18285.0
29	23180.0
30	28569.0
31	34320.0
32	44582.0
33	61152.0
34	89358.0
35	143678.0
36	279739.0
37	586283.0
38	903878.0
39	380123.0
40	656.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.962409523111496	19.1290264338207	12.955986535501621	23.952577507566183
2	15.655489069342387	19.843941729855583	39.9416640685558	24.558905132246224
3	18.472123947436824	25.153788369326634	29.56683970577036	26.80724797746618
4	12.413924041128388	16.357963002757074	38.15245478498128	33.075658171133256
5	13.16633373294914	38.184523820165026	35.148397144933966	13.500745301951872
6	31.44115389277201	37.39625430348215	17.632611965620054	13.529979838125787
7	28.254862897816572	32.30164053527987	21.885936246376332	17.557560320527212
8	25.986514321427386	36.12358427726767	19.869905641724586	18.01999575958036
9	25.726516567581058	16.861727307440933	20.277964094635934	37.13379203034208
10-11	24.467743925003084	26.78215104856601	28.258456191911446	20.49164883451946
12-13	24.945176996916825	24.15989457107916	28.25552641077057	22.639402021233444
14-15	22.278290301757618	25.938234102187828	25.221930023866673	26.56154557218788
16-17	22.34742460476785	29.24626755480015	25.807384054569372	22.59892378586263
18-19	22.328249084969364	28.281387003213222	27.571403672635782	21.818960239181635
20-21	23.189543385639762	27.603097270939347	27.18327891276609	22.024080430654802
22-23	23.113539355961347	27.264375763879066	27.123760341695835	22.498324538463752
24-25	22.740554853213737	27.4203143948928	27.116702514701508	22.722428237191956
26-27	22.555360744907613	27.428747330192373	27.240010611301035	22.775881313598983
28-29	22.652190576157317	27.5090468117541	27.266261201708332	22.572501410380255
30-31	22.785233729563913	27.730581081576304	26.973193432565274	22.51099175629451
32-33	22.543146250796607	27.60510935269675	27.284358126618347	22.567386269888303
34-35	22.726040118372705	27.32042769334998	27.28653541751762	22.666996770759688
36-37	22.863189234475975	27.572292678880956	26.8919667015648	22.672551385078265
38-39	22.68809804282581	27.784991482386506	26.968730616396364	22.558179858391327
40-41	22.573513257743343	27.50615064691953	27.254189974592997	22.66614612074413
42-43	22.57136651331332	27.475765731381063	27.19817441880213	22.75469333650349
44-45	22.53883074080351	27.561012584728278	27.25304464042459	22.647112034043623
46-47	22.62213384339272	27.577585585804627	27.16137755365644	22.638903017146212
48-49	22.59900661742542	27.537940156047807	27.129942464476976	22.733110762049797
50-51	22.512399986197902	27.5136577290938	27.13560184740709	22.838340437301206
52-53	22.650635495381753	27.438014371701552	27.160595800050523	22.750754332866173
54-55	22.617091641258536	27.75229316792751	27.05530527837144	22.575309912442517
56-57	22.542814710672083	27.467813767606934	27.148798517126156	22.840573004594827
58-59	22.49477832064928	27.17055686173272	27.3864500393045	22.948214778313496
60-61	22.72810240346222	27.499111043812448	27.042038286703878	22.730748266021457
62-63	22.42678883455312	27.50513353263203	27.194189659851393	22.873887972963455
64-65	22.48083676266654	27.3438663427996	27.209517644373005	22.965779250160853
66-67	22.3513993155513	27.350005783292954	27.202929582017195	23.095665319138554
68-69	22.62428053193977	27.426240445528517	27.151235422034297	22.798243600497415
70-71	22.54687825133736	27.210909405540225	27.28490135241333	22.957310990709086
72-73	22.34233625457668	27.36833712589055	27.309325900886527	22.98000071864624
74-75	22.3932444731107	27.44410452925421	27.221450446103677	22.941200551531416
76-77	22.447031036904736	27.31648768861878	27.154596739406163	23.081884535070323
78-79	22.40992216998039	27.397089342827318	27.2329986268866	22.959989860305686
80-81	22.370614980293322	27.28683545267796	27.30158395320238	23.040965613826334
82-83	22.28057228829509	27.327155806117602	27.392567231289366	22.99970467429794
84-85	22.29090649716216	27.267851808576992	27.300655556534803	23.140586137726043
86-87	22.4587447240978	27.219704567353673	27.314183663071635	23.007367045476894
88-89	22.190233514980726	27.25105953033864	27.334917414910436	23.223789539770195
90-91	22.22894949752795	27.180218163929336	27.375636871570297	23.215195466972418
92-93	22.387035177622064	27.250559646831253	27.286686653010744	23.07571852253594
94-95	22.176245531260484	26.97828924165095	27.453089742675324	23.392375484413243
96-97	22.29298019533491	26.97451837058718	27.43106370952562	23.30143772455229
98-99	22.173433665677074	27.12705712765775	27.352522466093504	23.346986740571676
100	22.320207928154673	26.85108254204324	27.36257293215879	23.46613659764329
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2530.0
1	1536.0
2	522.0
3	480.0
4	393.0
5	308.0
6	279.5
7	265.0
8	276.5
9	286.0
10	267.0
11	265.5
12	274.0
13	283.5
14	319.5
15	395.5
16	476.0
17	606.5
18	804.0
19	1064.5
20	1525.0
21	2142.5
22	3081.0
23	4728.0
24	6938.0
25	9666.0
26	13345.5
27	17899.5
28	23731.5
29	30579.5
30	36951.5
31	43359.0
32	49922.5
33	56849.5
34	63914.5
35	69996.5
36	76037.0
37	81828.5
38	86910.5
39	89933.0
40	92325.5
41	96120.5
42	98978.5
43	100471.0
44	99986.5
45	99490.5
46	100344.5
47	98920.5
48	96354.5
49	94390.0
50	92570.0
51	90152.0
52	84925.0
53	80896.0
54	78808.5
55	75396.0
56	70034.5
57	63259.5
58	57640.5
59	54484.0
60	50238.0
61	43741.5
62	38484.0
63	33702.5
64	27999.5
65	22505.0
66	18234.5
67	14406.5
68	11016.0
69	8460.5
70	6245.5
71	4672.0
72	3762.0
73	2811.5
74	2187.0
75	1674.5
76	1079.5
77	731.5
78	541.5
79	385.0
80	253.0
81	173.0
82	131.0
83	96.0
84	71.5
85	54.5
86	45.0
87	39.5
88	33.0
89	28.0
90	19.0
91	16.0
92	13.0
93	10.5
94	9.5
95	6.0
96	3.5
97	3.5
98	3.0
99	1.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07720211807458419
2	0.08009585780806003
3	0.08156059026574534
4	0.08370410117943115
5	0.08191784208469298
6	0.08156059026574534
7	0.08052455999079719
8	0.08048883480890243
9	0.07995295708048097
10-11	0.07970288080721763
12-13	0.07825601094047971
14-15	0.0767912784827944
16-17	0.0757016604350041
18-19	0.07532654602510909
20-21	0.07513005752468789
22-23	0.07700562957416297
24-25	0.07695204180132084
26-27	0.07602318707205698
28-29	0.07245066888258062
30-31	0.07729143102932108
32-33	0.07568379784405672
34-35	0.07318303511142327
36-37	0.06993204355899978
38-39	0.07148608897142199
40-41	0.07439769129584524
42-43	0.07961356785248072
44-45	0.08088181180974482
46-47	0.08386486449795759
48-49	0.08713371864132846
50-51	0.08822333668911875
52-53	0.08822333668911875
54-55	0.08820547409817137
56-57	0.08625845168490676
58-59	0.08547249768322196
60-61	0.08311463567816756
62-63	0.0835612004518521
64-65	0.08286455940490421
66-67	0.08204288022132465
68-69	0.08173921617521915
70-71	0.07930990380637523
72-73	0.07932776639732261
74-75	0.07882761385079592
76-77	0.0786489879413221
78-79	0.07922059085163832
80-81	0.08043524703606028
82-83	0.07981005635290192
84-85	0.07893478939648021
86-87	0.07970288080721763
88-89	0.0768805914375313
90-91	0.0741297524316345
92-93	0.0739332639312133
94-95	0.07418334020447666
96-97	0.07332593583900232
98-99	0.0729508214291073
100	0.07237921851879109
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2799146.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.75645155282757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.55945034479963	44.396051835425
2	13.943511363263283	16.385424997837596
3	4.511378730001016	7.952178173572843
4	2.018698377756097	4.74446213729591
5	1.1041423555578675	3.2437743410880375
6	0.6586030777410656	2.3218307897901633
7	0.4188891553463113	1.7228708253477283
8	0.31097762741710966	1.4617553519477335
9	0.22801407724233452	1.2057568274566723
>10	1.1621563640181827	12.036366892072996
>50	0.06316792229256109	2.5062781637389357
>100	0.020827933726658508	1.8962056529775122
>500	1.2178055860762664E-4	0.06003375139708541
>1k	6.089027930381316E-5	0.06701026005169289
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.5725181894763616E-5	0.0
4	0.0	0.0	0.0	3.5725181894763616E-5	0.0
5	0.0	0.0	0.0	3.5725181894763616E-5	0.0
6	0.0	0.0	0.0	3.5725181894763616E-5	0.0
7	0.0	0.0	0.0	3.5725181894763616E-5	0.0
8	0.0	0.0	0.0	3.5725181894763616E-5	0.0
9	0.0	0.0	0.0	3.5725181894763616E-5	0.0
10-11	0.0	0.0	0.0	7.145036378952723E-5	0.0
12-13	0.0	0.0	0.0	7.145036378952723E-5	0.0
14-15	0.0	0.0	0.0	8.931295473690904E-5	0.0
16-17	0.0	0.0	0.0	1.0717554568429085E-4	0.0
18-19	0.0	0.0	0.0	1.0717554568429085E-4	0.0
20-21	0.0	0.0	0.0	1.0717554568429085E-4	0.0
22-23	0.0	0.0	0.0	1.0717554568429085E-4	0.0
24-25	0.0	0.0	0.0	1.964885004211999E-4	0.0
26-27	0.0	0.0	0.0	3.929770008423998E-4	0.0
28-29	0.0	0.0	0.0	0.001786259094738181	0.0
30-31	0.0	0.0	0.0	0.007091448606110579	0.0
32-33	0.0	0.0	0.0	0.017951903902118718	0.0
34-35	0.0	0.0	0.0	0.033778159481499	0.0
36-37	0.0	0.0	0.0	0.06360868636362663	0.0
38-39	0.0	0.0	0.0	0.11303447551503208	0.0
40-41	0.0	0.0	0.0	0.16615782099254559	0.0
42-43	0.0	0.0	0.0	0.2218355169755347	0.0
44-45	0.0	0.0	0.0	0.2891596222562167	0.0
46-47	0.0	0.0	0.0	0.37473929548512297	0.0
48-49	0.0	0.0	0.0	0.4681606461399298	0.0
50-51	0.0	0.0	0.0	0.5688520713103211	0.0
52-53	0.0	3.5725181894763616E-5	0.0	0.671865633303872	0.0
54-55	0.0	3.5725181894763616E-5	0.0	0.7869721693688003	0.0
56-57	0.0	3.5725181894763616E-5	0.0	0.9214953417935328	0.0
58-59	0.0	3.5725181894763616E-5	0.0	1.0689688926551169	0.0
60-61	3.5725181894763616E-5	3.5725181894763616E-5	0.0	1.231304119184923	0.0
62-63	3.5725181894763616E-5	3.5725181894763616E-5	0.0	1.3972654516770473	0.0
64-65	3.5725181894763616E-5	3.5725181894763616E-5	0.0	1.570353957957177	0.0
66-67	3.5725181894763616E-5	3.5725181894763616E-5	0.0	1.7682536030632199	0.0
68-69	3.5725181894763616E-5	3.5725181894763616E-5	0.0	1.9918753791334929	0.0
70-71	3.5725181894763616E-5	3.5725181894763616E-5	0.0	2.2393437141185206	0.0
72-73	3.5725181894763616E-5	3.5725181894763616E-5	0.0	2.49443937543808	0.0
74-75	3.5725181894763616E-5	3.5725181894763616E-5	0.0	2.7475344265715327	0.0
76-77	3.5725181894763616E-5	3.5725181894763616E-5	0.0	3.0163485577386817	0.0
78-79	3.5725181894763616E-5	3.5725181894763616E-5	0.0	3.3302478684570223	0.0
80-81	3.5725181894763616E-5	3.5725181894763616E-5	0.0	3.672834500236858	0.0
82-83	3.5725181894763616E-5	3.5725181894763616E-5	0.0	4.0234235727611205	0.0
84-85	3.5725181894763616E-5	3.5725181894763616E-5	0.0	4.369654173094222	0.0
86-87	3.5725181894763616E-5	3.5725181894763616E-5	0.0	4.7340510284208115	0.0
88	3.5725181894763616E-5	3.5725181894763616E-5	0.0	5.029176756053453	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2390	0.0	36.18513	1
GTATCAA	4050	0.0	34.815807	1
TCAACGC	4390	0.0	30.620462	4
ATCAACG	4410	0.0	30.588177	3
CAACGCA	4570	0.0	29.517254	5
AACGCAG	4665	0.0	29.016905	6
GTACATG	10190	0.0	27.674982	1
TACATGG	10180	0.0	26.224716	2
TATCAAC	5270	0.0	26.131678	2
CGCGTAT	95	1.3358658E-8	24.735779	12-13
ACATGGG	10375	0.0	24.735163	3
ACGCAGA	5860	0.0	23.099634	7
CGCAGAG	6175	0.0	21.61681	8
GAGTACA	7465	0.0	21.155325	1
CATGGGG	7180	0.0	21.013134	4
AGTACAT	7525	0.0	19.86236	2
TCCGTAC	170	8.631154E-5	19.353493	3
TATACTG	1655	0.0	18.175745	5
GCAGAGT	7155	0.0	17.933426	9
CGCGAAC	85	0.0019204386	16.587523	28-29
>>END_MODULE