FastQCFastQC Report
Wed 25 May 2016
SRR1032989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032989_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799146
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60530.21624452600900418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49750.17773277992644898No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42850.051.204841
GTATCAA80650.043.3424571
ATCAACG96100.035.8903583
TCAACGC96000.035.878734
CAACGCA98050.035.1241875
AACGCAG99300.034.634046
TATCAAC101000.034.242322
ACGCAGA117900.028.8897847
GTACATG98500.027.9992771
CGCAGAG122450.027.8162948
TACATGG99250.026.644222
ACATGGG98950.026.1543623
GCAGAGT133350.025.2576839
TACACCG5200.023.524095
CATGGGG68400.023.0454884
GTGGTAT21550.021.8020671
ACACCGT5100.021.2174286
CAGAGTA128100.020.86558310-11
AGAGTAC121850.020.60580310-11
GAGTACT85400.020.40756412-13
Produced by FastQC (version 0.11.5)