FastQCFastQC Report
Wed 25 May 2016
SRR1032988_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032988_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2907205
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.033.490011
GTATCAA38900.032.8662151
TCAACGC39600.030.74224
ATCAACG41600.029.3772013
CAACGCA41700.029.1925265
AACGCAG42850.028.8478136
GTACATG93650.027.4542711
TACATGG94900.025.6067642
TATCAAC49150.025.4383262
ACATGGG95750.024.201293
ACGCAGA53500.022.75387
CGCAGAG55900.021.2720438
CATGGGG66400.020.1039144
TATACTG17250.017.9830275
GAGTACA69400.017.6771161
GCAGAGT67300.017.2494379
GTCGTAC2002.9925763E-416.4512331
CATGGGA32550.016.1732544
GTATTAG14550.016.1524121
GTACGGG2053.6120234E-416.0499821
Produced by FastQC (version 0.11.5)