##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032988_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2907205 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.811030181910116 33.0 31.0 34.0 30.0 34.0 2 31.842823949463487 34.0 31.0 34.0 30.0 34.0 3 31.91021444996139 34.0 31.0 34.0 30.0 34.0 4 35.25161349130866 37.0 35.0 37.0 33.0 37.0 5 35.29619995837927 37.0 35.0 37.0 33.0 37.0 6 35.37572513806216 37.0 37.0 37.0 35.0 37.0 7 35.29256657167279 37.0 36.0 37.0 33.0 37.0 8 35.297203327594715 37.0 37.0 37.0 33.0 37.0 9 36.97146124886274 39.0 38.0 39.0 35.0 39.0 10-11 36.96883432712863 39.0 38.0 39.0 34.5 39.0 12-13 36.94333285750403 39.0 38.0 39.0 34.5 39.0 14-15 38.449446977423335 41.0 39.0 41.0 35.0 41.0 16-17 38.4855921753024 41.0 39.0 41.0 35.0 41.0 18-19 38.49811313615655 41.0 39.0 41.0 35.0 41.0 20-21 38.53445680645156 41.0 39.0 41.0 35.0 41.0 22-23 38.53342041582895 41.0 39.0 41.0 35.0 41.0 24-25 38.56724328005765 41.0 39.0 41.0 35.0 41.0 26-27 38.543921395292045 41.0 39.0 41.0 34.5 41.0 28-29 38.5235380717906 41.0 39.0 41.0 34.0 41.0 30-31 38.481674150945665 41.0 39.0 41.0 34.0 41.0 32-33 38.42721531505346 41.0 39.0 41.0 34.0 41.0 34-35 38.360014859633225 41.0 39.0 41.0 34.0 41.0 36-37 38.2792145720718 41.0 39.0 41.0 34.0 41.0 38-39 38.18929538852609 41.0 39.0 41.0 33.5 41.0 40-41 38.10484933123051 41.0 38.5 41.0 33.0 41.0 42-43 37.99485622788899 40.5 38.0 41.0 33.0 41.0 44-45 37.877751482953556 40.0 38.0 41.0 33.0 41.0 46-47 37.7576084245865 40.0 38.0 41.0 33.0 41.0 48-49 37.62752041909669 40.0 38.0 41.0 33.0 41.0 50-51 37.49216618711098 40.0 37.5 41.0 32.0 41.0 52-53 37.3348203859033 40.0 37.0 41.0 32.0 41.0 54-55 37.168754009435176 40.0 37.0 41.0 31.5 41.0 56-57 36.987305848744754 40.0 36.0 41.0 31.0 41.0 58-59 36.776835482877885 40.0 36.0 41.0 31.0 41.0 60-61 36.53397748008826 39.0 35.0 41.0 31.0 41.0 62-63 36.28174053773297 39.0 35.0 41.0 31.0 41.0 64-65 36.00364938145057 38.5 35.0 41.0 30.0 41.0 66-67 35.710117105604866 38.0 35.0 40.0 30.0 41.0 68-69 35.39723583304239 37.0 35.0 40.0 30.0 41.0 70-71 35.060238098104534 37.0 35.0 39.5 29.5 41.0 72-73 34.76517445450183 36.0 34.5 39.0 29.5 41.0 74-75 34.481551524574286 36.0 34.0 39.0 29.0 41.0 76-77 34.158049569947764 35.0 34.0 38.5 29.0 40.0 78-79 33.79104844687595 35.0 34.0 37.0 29.0 39.0 80-81 33.46899823025896 35.0 34.0 37.0 29.0 39.0 82-83 33.154875043211604 35.0 34.0 36.5 28.5 39.0 84-85 32.98752100385077 35.0 34.0 36.0 29.0 37.0 86-87 32.75746533182215 35.0 34.0 36.0 28.5 37.0 88-89 32.52657432138429 35.0 34.0 35.5 28.0 37.0 90-91 32.33351002079317 35.0 34.0 35.0 27.0 36.0 92-93 32.14943218658471 35.0 34.0 35.0 27.0 36.0 94-95 31.97341811120991 35.0 34.0 35.0 27.0 36.0 96-97 31.807439791827548 35.0 33.5 35.0 26.5 36.0 98-99 31.634162365571058 35.0 33.0 35.0 25.5 35.0 100 31.51119890066232 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36418827436519763 1101 2 0.42492981039506006 1101 3 0.4287013938595905 1101 4 0.3895141715994015 1101 5 0.3684984678115697 1101 6 0.38528870630237577 1101 7 0.3786054776850918 1101 8 0.39054758607726825 1101 9 0.41791080544387427 1101 10-11 0.444403270846756 1101 12-13 0.24872610596009537 1101 14-15 0.25050862128508555 1101 16-17 0.3024097659087275 1101 18-19 0.24552628188296666 1101 20-21 0.2676940013851592 1101 22-23 0.3320171968625587 1101 24-25 0.3068921166069032 1101 26-27 0.31061724345434527 1101 28-29 0.31505551006432597 1101 30-31 0.28017870805427236 1101 32-33 0.292853313777627 1101 34-35 0.312458789997784 1101 36-37 0.29453422129770956 1101 38-39 0.2775965338381283 1101 40-41 0.31313251596611025 1101 42-43 0.3399571658727538 1101 44-45 0.3372843080755885 1101 46-47 0.3780488076313233 1101 48-49 0.3167263321056524 1101 50-51 0.3067034308414023 1101 52-53 0.2358575037127082 1101 54-55 0.28931250341663173 1101 56-57 0.2725701922206767 1101 58-59 0.3022449070510618 1101 60-61 0.3380564566387463 1101 62-63 0.33630943053625373 1101 64-65 0.2889668234851186 1101 66-67 0.2984865953724878 1101 68-69 0.3197393817738785 1101 70-71 0.3590623774858983 1101 72-73 0.37097548446273265 1101 74-75 0.28893242228333094 1101 76-77 0.26767363332849925 1101 78-79 0.33793793336220546 1101 80-81 0.35879894392711265 1101 82-83 0.37106621989374133 1101 84-85 0.3936660509776857 1101 86-87 0.3323240679342021 1101 88-89 0.320085854348072 1101 90-91 0.3655672733418385 1101 92-93 0.3736424982777322 1101 94-95 0.42091595909397483 1101 96-97 0.38369193578413885 1101 98-99 0.3274491057364841 1101 100 0.37895173117058434 1102 1 0.40304477874275335 1102 2 0.39178587490618 1102 3 0.43892966293609703 1102 4 0.3891911446899883 1102 5 0.33639823324886464 1102 6 0.2950169218683456 1102 7 0.4122937478809021 1102 8 0.3620088950137301 1102 9 0.4198344355986805 1102 10-11 0.4292489886783386 1102 12-13 0.4886850255774462 1102 14-15 0.5324909853904174 1102 16-17 0.5227607557554634 1102 18-19 0.5111031991065786 1102 20-21 0.4421487584718662 1102 22-23 0.44975481064609113 1102 24-25 0.48142748314425887 1102 26-27 0.4977453574975783 1102 28-29 0.5342332936677181 1102 30-31 0.5295418344834744 1102 32-33 0.5611936329360674 1102 34-35 0.5973519309288307 1102 36-37 0.5989776577149186 1102 38-39 0.6476186547994374 1102 40-41 0.6206809380925762 1102 42-43 0.6056022231191989 1102 44-45 0.5803275946632951 1102 46-47 0.5231292256222488 1102 48-49 0.5702309777039005 1102 50-51 0.5736234134431157 1102 52-53 0.5311391507768377 1102 54-55 0.5337122084704831 1102 56-57 0.5687246992315451 1102 58-59 0.5406206020743838 1102 60-61 0.6260198259763285 1102 62-63 0.5032012341948047 1102 64-65 0.6110454672826577 1102 66-67 0.6596450150345206 1102 68-69 0.5887458825677001 1102 70-71 0.5138069947677408 1102 72-73 0.5682455634325052 1102 74-75 0.5701736900019441 1102 76-77 0.5594748372664782 1102 78-79 0.5311491644874664 1102 80-81 0.471074064669466 1102 82-83 0.45752609042769876 1102 84-85 0.4414307342198498 1102 86-87 0.42158798877395043 1102 88-89 0.40733777900917545 1102 90-91 0.33652312862185596 1102 92-93 0.30384394335678166 1102 94-95 0.3566413606930041 1102 96-97 0.40583243579837713 1102 98-99 0.4154546943965691 1102 100 0.39580470287087266 1103 1 0.1330111473105049 1103 2 0.21260843908137517 1103 3 0.23858857168762881 1103 4 0.2364623304035689 1103 5 0.17280144401752295 1103 6 0.20935371313409945 1103 7 0.19565715711490128 1103 8 0.2527645316458944 1103 9 0.3002601102832827 1103 10-11 0.2970628294373512 1103 12-13 0.24373109856025366 1103 14-15 0.33203243796182136 1103 16-17 0.3681151765007371 1103 18-19 0.3111786370710803 1103 20-21 0.3439442457192641 1103 22-23 0.28279834380291646 1103 24-25 0.3054168682527276 1103 26-27 0.35523972681315286 1103 28-29 0.4089493241508748 1103 30-31 0.3840928680697573 1103 32-33 0.40768205844440786 1103 34-35 0.34668405111028733 1103 36-37 0.35809507661629425 1103 38-39 0.3299258201322175 1103 40-41 0.2806975617950016 1103 42-43 0.3917566684422269 1103 44-45 0.3746732504050456 1103 46-47 0.3753413451143288 1103 48-49 0.3768160544747232 1103 50-51 0.3285203714029805 1103 52-53 0.3286114450744435 1103 54-55 0.32855934777556683 1103 56-57 0.3609554880743673 1103 58-59 0.3551838008988497 1103 60-61 0.31144950859549425 1103 62-63 0.4065087590146774 1103 64-65 0.3967279335851899 1103 66-67 0.33192709869591397 1103 68-69 0.35209874884505865 1103 70-71 0.3480618064903567 1103 72-73 0.3103224696929132 1103 74-75 0.31727845136077093 1103 76-77 0.30769608799309367 1103 78-79 0.27021651371128996 1103 80-81 0.27806171453615747 1103 82-83 0.2823623027487585 1103 84-85 0.26779696783475515 1103 86-87 0.21602777805718887 1103 88-89 0.19706433465258755 1103 90-91 0.24746998841121126 1103 92-93 0.27146809332153055 1103 94-95 0.27347133266275847 1103 96-97 0.22434643340916338 1103 98-99 0.23228908736448162 1103 100 0.23478277041136053 1104 1 0.16290982637376672 1104 2 0.18870499870863355 1104 3 0.17919441916154 1104 4 0.1110773962643492 1104 5 0.14879029193196658 1104 6 0.0954289604653411 1104 7 0.09168640524375604 1104 8 0.12322247227284322 1104 9 0.20230772064364544 1104 10-11 0.19719600082267164 1104 12-13 0.19967142032700025 1104 14-15 0.2612442943583275 1104 16-17 0.27570470903844324 1104 18-19 0.23312925576846766 1104 20-21 0.18298873701710505 1104 22-23 0.20930254649163516 1104 24-25 0.22523040215811108 1104 26-27 0.26016690162824574 1104 28-29 0.265641535494332 1104 30-31 0.2364505151822911 1104 32-33 0.2802138953695845 1104 34-35 0.27652874354134127 1104 36-37 0.25160950591393316 1104 38-39 0.31886058160873176 1104 40-41 0.28005637394167593 1104 42-43 0.24982767293063546 1104 44-45 0.17890406186359797 1104 46-47 0.22506026331543438 1104 48-49 0.23221370418374931 1104 50-51 0.22413654630206992 1104 52-53 0.2824406291588133 1104 54-55 0.27878158640421447 1104 56-57 0.24643469492858827 1104 58-59 0.23593394640179355 1104 60-61 0.3484594790027131 1104 62-63 0.33493667387225656 1104 64-65 0.3201019578118789 1104 66-67 0.2548068498033942 1104 68-69 0.24065736936949378 1104 70-71 0.22201710838839261 1104 72-73 0.18521790105781832 1104 74-75 0.1450952151908993 1104 76-77 0.15312775902916087 1104 78-79 0.14402151259286455 1104 80-81 0.1635370278378403 1104 82-83 0.1403104076972923 1104 84-85 0.09650624684810083 1104 86-87 0.0599597180455973 1104 88-89 0.05077785942149404 1104 90-91 0.05279114318777545 1104 92-93 0.010958012973460995 1104 94-95 0.057676682071054586 1104 96-97 0.10054228159818379 1104 98-99 0.1256136539630468 1104 100 0.14551698563731463 1105 1 -0.0368448603049103 1105 2 -0.038001030506251254 1105 3 -0.08308857530404978 1105 4 -0.061913037777401314 1105 5 -0.15428074359726907 1105 6 -0.08743866124422794 1105 7 -0.08716511843956454 1105 8 -0.10336833728952399 1105 9 -0.08759151556595413 1105 10-11 -0.1133653631871141 1105 12-13 -0.1180153815231435 1105 14-15 -0.06379034539617834 1105 16-17 -0.07656043092615761 1105 18-19 -0.072750826935156 1105 20-21 -0.0656966056113717 1105 22-23 0.003804319215298335 1105 24-25 -0.006099525794382998 1105 26-27 0.02377969450944306 1105 28-29 0.033947597525227025 1105 30-31 0.004406571952166871 1105 32-33 0.04114817221627476 1105 34-35 0.06184396473921083 1105 36-37 0.07636672083448559 1105 38-39 0.04555294695456524 1105 40-41 0.08049312346574311 1105 42-43 -0.009199343691676631 1105 44-45 -0.008169844157613682 1105 46-47 -0.04450403936363756 1105 48-49 -0.07065296986122149 1105 50-51 -0.030101355236709537 1105 52-53 0.03766059642916275 1105 54-55 0.0282419061876098 1105 56-57 0.030028836770569 1105 58-59 -0.010732041143121762 1105 60-61 -0.02983777451984082 1105 62-63 0.022020863304277327 1105 64-65 -0.05112627459365626 1105 66-67 -0.006098764166232229 1105 68-69 -0.042068932141013704 1105 70-71 -0.03127773052112559 1105 72-73 -0.04928837932227026 1105 74-75 -0.004757658175890356 1105 76-77 -0.06700052339580509 1105 78-79 -0.07431120676592684 1105 80-81 -0.057681637018639265 1105 82-83 -0.08457804286199888 1105 84-85 -0.05469801441552846 1105 86-87 -0.13468743710846098 1105 88-89 -0.07978097748270585 1105 90-91 -0.13459703182156346 1105 92-93 -0.17965332954911162 1105 94-95 -0.1422544986167722 1105 96-97 -0.08805601224192827 1105 98-99 -0.08896073441263397 1105 100 -0.10640811431629871 1106 1 0.12664984270947244 1106 2 0.13696850687962225 1106 3 0.05798082260864135 1106 4 0.028781319604107125 1106 5 0.08161626314068826 1106 6 0.07852278652198663 1106 7 0.025343706590135184 1106 8 0.03280539657971815 1106 9 0.0705702895087299 1106 10-11 0.07513144974456054 1106 12-13 0.1271163122987744 1106 14-15 0.09005651483918342 1106 16-17 0.12279780985322475 1106 18-19 0.14425687196717973 1106 20-21 0.20315930431068807 1106 22-23 0.13164409843705727 1106 24-25 0.18313578551899923 1106 26-27 0.19190501004047178 1106 28-29 0.21531985930627684 1106 30-31 0.20413317368002737 1106 32-33 0.19186431968658724 1106 34-35 0.21488643267338148 1106 36-37 0.2604682098238982 1106 38-39 0.24705980506033853 1106 40-41 0.2837485345290105 1106 42-43 0.285280336492967 1106 44-45 0.2487515317970619 1106 46-47 0.2733448059431254 1106 48-49 0.2857955823747602 1106 50-51 0.23293629377756275 1106 52-53 0.23857290608019355 1106 54-55 0.2562052412912337 1106 56-57 0.23786919102677473 1106 58-59 0.2613353706049466 1106 60-61 0.3177229971107991 1106 62-63 0.3099742030846997 1106 64-65 0.33577390489143255 1106 66-67 0.3480501148773456 1106 68-69 0.38378671584519 1106 70-71 0.2802449978796062 1106 72-73 0.3114671099063244 1106 74-75 0.29578841684227797 1106 76-77 0.36129049388824797 1106 78-79 0.33139538704264027 1106 80-81 0.34327337932611357 1106 82-83 0.3053639978193914 1106 84-85 0.34878327258591924 1106 86-87 0.38127034289997397 1106 88-89 0.3508313587773273 1106 90-91 0.33200942749890316 1106 92-93 0.31484992595488137 1106 94-95 0.3753023909847073 1106 96-97 0.3427102495902794 1106 98-99 0.28588755378851616 1106 100 0.2943753209307758 1107 1 0.09882321681191542 1107 2 0.14623256168397702 1107 3 0.10817379118595483 1107 4 0.11312682572686583 1107 5 0.10439839910441151 1107 6 0.09673513032150538 1107 7 0.007962215019453822 1107 8 0.07090463957053572 1107 9 0.08486425237068573 1107 10-11 0.014392873516854365 1107 12-13 0.08087296029679436 1107 14-15 0.10364839707720819 1107 16-17 0.1281624978571969 1107 18-19 0.1687784736530631 1107 20-21 0.12906291643026435 1107 22-23 0.13946176749580985 1107 24-25 0.12542491559048585 1107 26-27 0.14100190933562118 1107 28-29 0.1703421931806801 1107 30-31 0.15372459790027193 1107 32-33 0.1166970818265014 1107 34-35 0.1526469722489665 1107 36-37 0.16626037234865976 1107 38-39 0.041878644131081444 1107 40-41 0.08038431209602237 1107 42-43 0.046290911920031874 1107 44-45 0.07129911985507675 1107 46-47 0.0806996263180153 1107 48-49 0.04706257327661234 1107 50-51 0.018989589381256167 1107 52-53 -0.026908456865754715 1107 54-55 0.029391001491120505 1107 56-57 -0.013617137520100187 1107 58-59 0.08325011572475916 1107 60-61 0.1683742404885109 1107 62-63 0.10836292674147074 1107 64-65 0.10041945881966541 1107 66-67 0.10404051223977007 1107 68-69 0.12785792428723397 1107 70-71 0.1318267013385892 1107 72-73 0.09361487550654601 1107 74-75 0.10811961720029473 1107 76-77 -0.00991626442323934 1107 78-79 0.008311783900879277 1107 80-81 0.08105355722710073 1107 82-83 0.06308950732221064 1107 84-85 0.1227751010703173 1107 86-87 0.07255699520646175 1107 88-89 0.033828345574228535 1107 90-91 0.10817589629615298 1107 92-93 0.1254055992960943 1107 94-95 0.06778145261683477 1107 96-97 0.05443507993434693 1107 98-99 0.11531970195800056 1107 100 0.11311761627125705 1108 1 0.1261092073186738 1108 2 0.16552574686974353 1108 3 0.16248060374214646 1108 4 0.07712666685764447 1108 5 0.1061668272537375 1108 6 0.11647180861807982 1108 7 0.15343108033131614 1108 8 0.13858378503153546 1108 9 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2214 70-71 -0.18023624602552246 2214 72-73 -0.16779896498866975 2214 74-75 -0.1951571328953321 2214 76-77 -0.1393369415489687 2214 78-79 -0.14440266179713745 2214 80-81 -0.15857746160699548 2214 82-83 -0.13423645999171896 2214 84-85 -0.09999806089742691 2214 86-87 -0.07362170243128219 2214 88-89 -0.12547148946873676 2214 90-91 -0.14081871816716784 2214 92-93 -0.1583945777201592 2214 94-95 -0.10902753471955862 2214 96-97 -0.17329168858681498 2214 98-99 -0.20610061649228228 2214 100 -0.16244184167160824 2215 1 -0.10343679564563502 2215 2 -0.11961525589171629 2215 3 -0.14413243281304844 2215 4 -0.040665632473711355 2215 5 -0.08660637479521682 2215 6 -0.09006353993083138 2215 7 -0.09523468235092736 2215 8 -0.1167220628907586 2215 9 -0.14490210048964514 2215 10-11 -0.08727462957210008 2215 12-13 -0.16896282969299392 2215 14-15 -0.19407383337227913 2215 16-17 -0.23622623835326095 2215 18-19 -0.21501083481850713 2215 20-21 -0.2612796213410533 2215 22-23 -0.23084964881509507 2215 24-25 -0.1734444575444769 2215 26-27 -0.1861664946241106 2215 28-29 -0.21329583520274298 2215 30-31 -0.20446863685418037 2215 32-33 -0.2688940974841074 2215 34-35 -0.25638787975255894 2215 36-37 -0.22372551507240956 2215 38-39 -0.25712989806503117 2215 40-41 -0.1979068314762884 2215 42-43 -0.2428140857706893 2215 44-45 -0.2789245850365205 2215 46-47 -0.2230343713949381 2215 48-49 -0.1612622622246036 2215 50-51 -0.1796990493031032 2215 52-53 -0.23977522919000194 2215 54-55 -0.1851022257295014 2215 56-57 -0.20919578515024284 2215 58-59 -0.20173407270454646 2215 60-61 -0.15204064967870323 2215 62-63 -0.09932338610851588 2215 64-65 -0.12979556233626965 2215 66-67 -0.2585492408142329 2215 68-69 -0.19029323822481814 2215 70-71 -0.1744301396676775 2215 72-73 -0.1575259315171209 2215 74-75 -0.10737046391169258 2215 76-77 -0.08315061029589543 2215 78-79 -0.11565199059507592 2215 80-81 -0.10127118759226761 2215 82-83 -0.13407736573474693 2215 84-85 -0.10577881299811054 2215 86-87 -0.18097601979793154 2215 88-89 -0.06854069198616486 2215 90-91 -0.09226642771769633 2215 92-93 -0.056452504635998935 2215 94-95 -0.07042919960463223 2215 96-97 -0.0652035063321108 2215 98-99 -0.057097576279613094 2215 100 -0.04399679750590124 2216 1 0.03580471823402931 2216 2 -0.06927555659081008 2216 3 -0.06110838014962994 2216 4 0.0587458237230436 2216 5 0.03676500902751201 2216 6 0.0017224200499015296 2216 7 0.05119714031334155 2216 8 0.015161858174970178 2216 9 -0.025759081565453812 2216 10-11 -0.05747801763038751 2216 12-13 -0.052397188103768144 2216 14-15 -0.21052454758310546 2216 16-17 -0.19561034682688927 2216 18-19 -0.19329478835904723 2216 20-21 -0.13910612671060818 2216 22-23 -0.13463270210615974 2216 24-25 -0.19468147280460357 2216 26-27 -0.2223882931922816 2216 28-29 -0.21422993566831394 2216 30-31 -0.21903635113333308 2216 32-33 -0.20930419300266578 2216 34-35 -0.2578369024514586 2216 36-37 -0.22827119405469887 2216 38-39 -0.21924065323175057 2216 40-41 -0.20655155698801764 2216 42-43 -0.1560631745256913 2216 44-45 -0.1359805894336219 2216 46-47 -0.12652400338114234 2216 48-49 -0.19422748540322488 2216 50-51 -0.13824179310419282 2216 52-53 -0.15952094086840418 2216 54-55 -0.1402488063720213 2216 56-57 -0.12848718825869554 2216 58-59 -0.11805267471626024 2216 60-61 -0.1562349215526595 2216 62-63 -0.13025799969830842 2216 64-65 -0.10821025190718103 2216 66-67 -0.12537348456455533 2216 68-69 -0.15280719700171375 2216 70-71 -0.13554420981051152 2216 72-73 -0.13099198053529193 2216 74-75 -0.13567707928621786 2216 76-77 -0.19268001867611417 2216 78-79 -0.131628860172178 2216 80-81 -0.07664402558967964 2216 82-83 -0.0873995546369315 2216 84-85 -0.11336001594385436 2216 86-87 -0.06565712484898256 2216 88-89 0.03654268862559462 2216 90-91 -0.02982245991478294 2216 92-93 0.007161102865183011 2216 94-95 -0.03460776932410781 2216 96-97 -0.003434345291598362 2216 98-99 0.007717516432666827 2216 100 -0.031818326538637365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36189.0 3 5402.0 4 3464.0 5 3095.0 6 2398.0 7 2869.0 8 3139.0 9 3552.0 10 3689.0 11 3945.0 12 4311.0 13 4764.0 14 5052.0 15 5351.0 16 5406.0 17 5277.0 18 5239.0 19 4993.0 20 5140.0 21 5554.0 22 6382.0 23 9388.0 24 14492.0 25 19688.0 26 20565.0 27 17720.0 28 18417.0 29 22984.0 30 28354.0 31 34211.0 32 44867.0 33 60593.0 34 89735.0 35 142370.0 36 277428.0 37 591566.0 38 960526.0 39 428288.0 40 802.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.91359470046027 19.30780201218104 12.90647492415681 23.872128363201877 2 15.717383424139328 19.836270371106036 40.37249333972711 24.073852865027533 3 18.51223151159052 25.296312335750194 29.35332134887778 26.838134803781504 4 12.44433367024602 16.336363711474217 38.45961591953754 32.75968669874222 5 13.055444725850531 38.20986712943185 35.18644807644576 13.548240068271863 6 31.472112847113447 37.67924111307639 17.627610859772123 13.221035180038045 7 28.173416071751916 32.367022869649816 21.935090319866653 17.52447073873162 8 25.613018048876206 36.556117443345535 19.94182223767509 17.889042270103168 9 25.42985989190678 17.157630211022752 20.55540638231953 36.85710351475094 10-11 24.212277796510424 27.012932067958722 28.383938162638746 20.390851972892108 12-13 24.739362400680083 24.350972746974254 28.443978342435923 22.465686509909748 14-15 22.010267153919873 26.250141136361133 25.396455480772406 26.343136228946584 16-17 22.011669434858565 29.57983149886834 25.956385917505013 22.45211314876808 18-19 21.90011906896947 28.587034459778337 27.845719110827773 21.66712736042442 20-21 22.92655640474656 27.90686226840995 27.33399826681273 21.832583060030757 22-23 22.69167575732436 27.63054488524429 27.38835874873537 22.28942060869598 24-25 22.383890596393478 27.705257838547613 27.455945148109983 22.454906416948926 26-27 22.24530109756805 27.69864924896218 27.49081888948193 22.56523076398784 28-29 22.328966676431033 27.774712309745446 27.500661762453266 22.395659251370255 30-31 22.448435487021847 28.066551289984176 27.209591124199754 22.275422098794227 32-33 22.132815793056405 27.88347495295649 27.61228953913798 22.37141971484912 34-35 22.393671547505043 27.57300776358283 27.62560488409701 22.407715804815115 36-37 22.5434597445226 27.98043913501654 27.13927168581316 22.3368294346477 38-39 22.382487256264987 28.097229302553085 27.29267615165191 22.22760728953002 40-41 22.219177733250213 27.756692014332202 27.614182392351022 22.409947860066566 42-43 22.14411743899465 27.762085103958633 27.54144291625666 22.55235454079006 44-45 22.249419600564014 27.87480171407169 27.57467048734275 22.301108198021545 46-47 22.31233356220726 27.947400192405574 27.423939294991413 22.31632695039575 48-49 22.174206345176223 27.801169858407576 27.53627254764287 22.488351248773327 50-51 22.205274459087878 27.74585607969492 27.50402196182226 22.54484749939494 52-53 22.312492393728665 27.693506555207286 27.539358527312256 22.45464252375179 54-55 22.364930765107445 28.061915317951012 27.331627578556926 22.241526338384613 56-57 22.181642919540923 27.830535461465967 27.459538082184707 22.528283536808402 58-59 22.073836100361078 27.43009217764633 27.820432691521518 22.67563903047107 60-61 22.406783704526596 27.864564820789827 27.314036022938932 22.414615451744645 62-63 22.110040878601623 27.846608766359232 27.44918317672297 22.594167178316184 64-65 22.14496064427412 27.594053463336582 27.58968146553497 22.671304426854324 66-67 22.009441396112113 27.658667333134357 27.484218726709347 22.847672544044183 68-69 22.40745091660492 27.770069477972825 27.407059333827334 22.41542027159493 70-71 22.331977882484562 27.55474085070677 27.45102089115515 22.66226037565352 72-73 22.038190819153172 27.662766756892314 27.650873219713045 22.648169204241466 74-75 22.129120905127937 27.758700250382145 27.50761241249663 22.604566431993288 76-77 22.163489342366162 27.675320830579427 27.39082465851509 22.770365168539325 78-79 22.20349359875233 27.677941190898338 27.486130099349676 22.632435110999655 80-81 22.07491239840918 27.570877337806678 27.644889881383712 22.709320382400435 82-83 22.025986856826155 27.605949196710423 27.701287122237023 22.6667768242264 84-85 22.040733498327256 27.559234556260844 27.568288051228322 22.831743894183575 86-87 22.20946229829753 27.614242484797817 27.541899912510754 22.634395304393895 88-89 21.86416041955141 27.60714042906946 27.599498452241516 22.92920069913761 90-91 21.974381150309586 27.48743368315068 27.659304582047874 22.878880584491863 92-93 22.134942463056223 27.59673538513437 27.52551556061946 22.742806591189947 94-95 21.866115769048463 27.36304407766128 27.672123664880484 23.098716488409774 96-97 22.012436056440034 27.29836243214952 27.693133560588773 22.996067950821676 98-99 21.925739205325232 27.40509577424355 27.651747109320706 23.017417911110513 100 22.06885038242563 27.211872749678157 27.642592095389553 23.07668477250666 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2725.0 1 1701.5 2 621.0 3 494.0 4 405.0 5 345.0 6 275.5 7 274.0 8 303.5 9 305.5 10 293.0 11 292.0 12 325.0 13 335.5 14 352.0 15 414.5 16 496.0 17 640.5 18 911.0 19 1261.0 20 1775.5 21 2562.0 22 3698.5 23 5507.5 24 7916.0 25 10952.5 26 14771.0 27 19582.5 28 25692.0 29 32829.5 30 40290.5 31 48246.5 32 56420.0 33 64163.5 34 71710.5 35 78518.0 36 85196.5 37 92116.0 38 97265.5 39 99498.0 40 101413.0 41 103673.5 42 104834.5 43 104844.0 44 103839.5 45 103180.0 46 102357.0 47 100047.0 48 97845.5 49 95548.5 50 93119.0 51 90417.0 52 84780.0 53 80003.0 54 77779.0 55 74536.5 56 69110.0 57 62205.5 58 56139.0 59 52525.5 60 48266.0 61 42301.5 62 37097.0 63 32193.5 64 26577.0 65 21098.0 66 16986.5 67 13445.5 68 10159.5 69 7691.0 70 5748.0 71 4356.5 72 3450.0 73 2629.0 74 1956.0 75 1407.0 76 990.0 77 691.0 78 529.5 79 361.0 80 229.0 81 166.5 82 132.0 83 94.5 84 71.5 85 59.5 86 46.5 87 35.0 88 26.0 89 21.5 90 18.5 91 17.0 92 12.5 93 7.0 94 4.5 95 3.0 96 2.0 97 2.0 98 1.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07560526347471197 2 0.07890740419062295 3 0.08018010425821365 4 0.08245032600040245 5 0.08076485834332288 6 0.07976733666872476 7 0.0793545690792359 8 0.07962974747222848 9 0.07928577448098775 10-11 0.07892460284018499 12-13 0.07778949196909059 14-15 0.07612122296157306 16-17 0.07498611209047865 18-19 0.07450454990274163 20-21 0.07404018636456665 22-23 0.0759492364659527 24-25 0.07631040810675546 26-27 0.07538168103040549 28-29 0.07168397137456767 30-31 0.07661998379887211 32-33 0.07503770803916476 34-35 0.07233752005792506 36-37 0.06936215368369275 38-39 0.07058325780259735 40-41 0.0735414255272676 42-43 0.0782710541568276 44-45 0.07942336367748404 46-47 0.08267390844470893 48-49 0.08580406266499954 50-51 0.08695637218565598 52-53 0.08700796813434208 54-55 0.08695637218565598 56-57 0.08487533558864958 58-59 0.0837058274184311 60-61 0.08122922188149787 62-63 0.08202035976135154 64-65 0.08098844078762936 66-67 0.08042088535208215 68-69 0.08005971371127939 70-71 0.07799587576383503 72-73 0.07794427981514891 74-75 0.07723913518310542 76-77 0.07722193653354338 78-79 0.07785828656733873 80-81 0.0788386095923748 82-83 0.07825385550726557 84-85 0.0773767243796017 86-87 0.07830545145595168 88-89 0.07558806482514993 90-91 0.0728362808952241 92-93 0.07275028764741392 94-95 0.07319745253602687 96-97 0.07237191735704912 98-99 0.07195914976756025 100 0.07103042269121028 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2907205.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.738454630372296 #Duplication Level Percentage of deduplicated Percentage of total 1 77.63255348356786 48.70546434568773 2 13.531842050668494 16.979337171224586 3 4.088072976347483 7.694381428566829 4 1.6262085461665556 4.081032443727766 5 0.8615644936003974 2.702661244644411 6 0.5208987310525542 1.9608228843095508 7 0.33831315592844863 1.4857671208852528 8 0.24172857446504847 1.2132541761552014 9 0.17516434424437305 0.9890586237811062 >10 0.9161050455544762 10.12277402259188 >50 0.05068825569797117 2.1886558816658748 >100 0.016476149768255878 1.6599264834104264 >500 2.7442352730017996E-4 0.11359715909168554 >1k 1.0976941088981363E-4 0.10326701425767446 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.439729912407278E-5 0.0 4 0.0 0.0 0.0 3.439729912407278E-5 0.0 5 0.0 0.0 0.0 3.439729912407278E-5 0.0 6 0.0 0.0 0.0 3.439729912407278E-5 0.0 7 0.0 0.0 0.0 3.439729912407278E-5 0.0 8 0.0 0.0 0.0 3.439729912407278E-5 0.0 9 0.0 0.0 0.0 1.375891964962911E-4 0.0 10-11 0.0 0.0 0.0 1.375891964962911E-4 0.0 12-13 0.0 0.0 0.0 1.375891964962911E-4 0.0 14-15 0.0 1.719864956203639E-5 0.0 1.375891964962911E-4 0.0 16-17 0.0 3.439729912407278E-5 0.0 1.375891964962911E-4 0.0 18-19 0.0 3.439729912407278E-5 0.0 1.375891964962911E-4 0.0 20-21 0.0 3.439729912407278E-5 0.0 1.375891964962911E-4 0.0 22-23 0.0 3.439729912407278E-5 0.0 2.751783929925822E-4 0.0 24-25 0.0 3.439729912407278E-5 0.0 4.299662390509097E-4 0.0 26-27 0.0 3.439729912407278E-5 0.0 6.363500337953464E-4 0.0 28-29 0.0 3.439729912407278E-5 0.0 0.0012211041189045836 0.0 30-31 0.0 3.439729912407278E-5 0.0 0.004695231330435934 0.0 32-33 0.0 3.439729912407278E-5 0.0 0.01356973450444671 0.0 34-35 0.0 3.439729912407278E-5 0.0 0.025883967590864768 0.0 36-37 0.0 3.439729912407278E-5 0.0 0.047623060637278766 0.0 38-39 0.0 3.439729912407278E-5 0.0 0.08731754382645875 0.0 40-41 0.0 3.439729912407278E-5 0.0 0.1355425571984088 0.0 42-43 0.0 3.439729912407278E-5 0.0 0.18407714626247546 0.0 44-45 0.0 3.439729912407278E-5 0.0 0.2406607033215752 0.0 46-47 0.0 3.439729912407278E-5 0.0 0.3107280016373114 0.0 48-49 0.0 3.439729912407278E-5 0.0 0.39164764782669265 0.0 50-51 0.0 3.439729912407278E-5 0.0 0.4824737161638068 0.0 52-53 0.0 3.439729912407278E-5 0.0 0.5723882560741331 0.0 54-55 0.0 3.439729912407278E-5 0.0 0.6705065518255506 0.0 56-57 0.0 3.439729912407278E-5 0.0 0.7879045337360111 0.0 58-59 0.0 3.439729912407278E-5 0.0 0.9200073610220125 0.0 60-61 0.0 3.439729912407278E-5 0.0 1.064286832197936 0.0 62-63 0.0 3.439729912407278E-5 0.0 1.214190261780645 0.0 64-65 0.0 3.439729912407278E-5 0.0 1.3717298917688985 0.0 66-67 0.0 6.879459824814555E-5 0.0 1.5460726023792613 0.0 68-69 0.0 1.0319189737221833E-4 0.0 1.7424639817281546 0.0 70-71 0.0 1.0319189737221833E-4 0.0 1.9614543866015641 0.0 72-73 0.0 1.0319189737221833E-4 0.0 2.1895944730419767 0.0 74-75 0.0 1.0319189737221833E-4 0.0 2.4216558515825337 0.0 76-77 0.0 1.0319189737221833E-4 0.0 2.671638222966733 0.0 78-79 0.0 1.375891964962911E-4 0.0 2.958116128721573 0.0 80-81 0.0 1.375891964962911E-4 0.0 3.266419120770637 0.0 82-83 0.0 1.375891964962911E-4 0.0 3.592402324569475 0.0 84-85 0.0 1.375891964962911E-4 0.0 3.9109213144583888 0.0 86-87 0.0 1.547878460583275E-4 0.0 4.236990511505036 0.0 88 0.0 1.719864956203639E-4 0.0 4.505908596057037 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2400 0.0 33.49001 1 GTATCAA 3890 0.0 32.866215 1 TCAACGC 3960 0.0 30.7422 4 ATCAACG 4160 0.0 29.377201 3 CAACGCA 4170 0.0 29.192526 5 AACGCAG 4285 0.0 28.847813 6 GTACATG 9365 0.0 27.454271 1 TACATGG 9490 0.0 25.606764 2 TATCAAC 4915 0.0 25.438326 2 ACATGGG 9575 0.0 24.20129 3 ACGCAGA 5350 0.0 22.7538 7 CGCAGAG 5590 0.0 21.272043 8 CATGGGG 6640 0.0 20.103914 4 TATACTG 1725 0.0 17.983027 5 GAGTACA 6940 0.0 17.677116 1 GCAGAGT 6730 0.0 17.249437 9 GTCGTAC 200 2.9925763E-4 16.451233 1 CATGGGA 3255 0.0 16.173254 4 GTATTAG 1455 0.0 16.152412 1 GTACGGG 205 3.6120234E-4 16.049982 1 >>END_MODULE