FastQCFastQC Report
Wed 25 May 2016
SRR1032988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2907205
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62600.21532709251669557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48660.16737725753773813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39700.051.8357961
GTATCAA76800.046.1271131
TCAACGC93850.037.3864444
ATCAACG94300.037.158163
CAACGCA96000.036.6433455
AACGCAG98000.035.942896
TATCAAC99800.035.251752
ACGCAGA114750.030.4499037
CGCAGAG118750.029.4237168
GTCGTAC2250.029.2342341
GTACATG94400.027.1748311
GCAGAGT132900.026.1215219
TACATGG96350.025.4328822
ACATGGG95700.024.6718333
GCGTAGA1503.3024942E-521.9256761
GTGGTAT19150.021.5903681
AGAGTAC122650.021.41032410-11
CAGAGTA126950.021.27718210-11
CGTACAC2458.436473E-921.1171953
GAGTACT87250.020.4865212-13
Produced by FastQC (version 0.11.5)