FastQCFastQC Report
Wed 25 May 2016
SRR1032987_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032987_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310913
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73762.372367832802102No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11480.3692351236519541No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7200.23157603573990154No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5440.17496856033681446No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4350.13991052159285716No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4120.1325129537844992No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3940.12672355289100168No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3700.1190043516996716No Hit
GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3460.11128515050834156No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3270.10517411623187194No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3220.10356594931701152No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3210.10324431593403943No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG250.005230181456.4061857
TGCACGA550.00271251134.1855665
GAGAACA4300.032.7942961
TAGACCA600.004154119631.3367675
CGATGCT600.004154119631.3367679
CCGTATC1401.8824721E-830.21273294
GCGCCGG1252.3548637E-730.07845794
TACATGA2200.029.912372
ATGCACG650.006144056628.9262474
CTATAAC909.4959117E-426.1139743
ATGGGAC1101.01523634E-425.6391743
GTACCCA1101.01523634E-425.6391741
TAAACAG2202.4301698E-923.5025775
TAACAAC1802.148081E-723.5025771
CATGGGG6050.023.308344
CAACACA10000.023.0325246
TACCACA2253.1523086E-922.9802972
TGGACAT1050.002330204522.3834065
AGGACAG1050.002330204522.3834065
GTGTAAG1050.002330204522.3834061
Produced by FastQC (version 0.11.5)