##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032987_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 310913 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.072541193195523 33.0 31.0 34.0 22.0 34.0 2 30.024222210071628 34.0 31.0 34.0 20.0 34.0 3 30.24521007484409 34.0 31.0 34.0 23.0 34.0 4 33.180616442541805 37.0 35.0 37.0 20.0 37.0 5 33.61387590740818 37.0 35.0 37.0 27.0 37.0 6 33.46457690736637 37.0 35.0 37.0 25.0 37.0 7 33.596578464071946 37.0 35.0 37.0 27.0 37.0 8 33.32484971680181 37.0 35.0 37.0 22.0 37.0 9 34.63643527288984 39.0 37.0 39.0 17.0 39.0 10-11 34.860914468034466 39.0 37.0 39.0 20.5 39.0 12-13 34.86434629623078 39.0 37.0 39.0 21.5 39.0 14-15 36.315623663211255 41.0 38.0 41.0 25.0 41.0 16-17 36.5072287102823 41.0 38.0 41.0 27.0 41.0 18-19 36.53922801555419 41.0 38.0 41.0 28.5 41.0 20-21 36.6243418576901 41.0 38.0 41.0 29.0 41.0 22-23 36.62510091247391 41.0 37.0 41.0 29.0 41.0 24-25 36.61947554460572 41.0 37.0 41.0 29.0 41.0 26-27 36.39280280978923 41.0 37.0 41.0 28.5 41.0 28-29 36.12670586305494 41.0 37.0 41.0 27.0 41.0 30-31 35.836573253611135 41.0 37.0 41.0 26.5 41.0 32-33 35.57633003444693 40.0 37.0 41.0 25.0 41.0 34-35 35.3110403875039 40.0 36.0 41.0 21.5 41.0 36-37 35.05814809930752 40.0 36.0 41.0 5.5 41.0 38-39 34.812601917578235 40.0 36.0 41.0 2.0 41.0 40-41 34.65363944254502 40.0 36.0 41.0 2.0 41.0 42-43 34.47434330504032 40.0 35.0 41.0 2.0 41.0 44-45 34.34493250523458 40.0 35.0 41.0 2.0 41.0 46-47 34.20631977434202 40.0 35.0 41.0 2.0 41.0 48-49 34.05612824166246 40.0 35.0 41.0 2.0 41.0 50-51 33.93140685658046 40.0 35.0 41.0 2.0 41.0 52-53 33.79319616741661 40.0 35.0 41.0 2.0 41.0 54-55 33.667286346984525 40.0 35.0 41.0 2.0 41.0 56-57 33.49861536828631 40.0 35.0 41.0 2.0 41.0 58-59 33.304811957042645 39.0 34.5 41.0 2.0 41.0 60-61 33.07538764863483 39.0 34.0 41.0 2.0 41.0 62-63 32.834913625355 39.0 34.0 41.0 2.0 41.0 64-65 32.554349609054626 38.0 34.0 40.5 2.0 41.0 66-67 32.2442805543673 37.5 33.0 40.0 2.0 41.0 68-69 31.891543615094896 37.0 33.0 40.0 2.0 41.0 70-71 31.494884421043828 36.0 32.5 39.5 2.0 41.0 72-73 31.162564768922493 36.0 32.0 39.0 2.0 41.0 74-75 30.846828534027203 35.0 32.0 39.0 2.0 40.5 76-77 30.456439582777175 35.0 31.5 37.5 2.0 40.0 78-79 30.02971892458662 35.0 31.0 37.0 2.0 39.0 80-81 29.626048122786763 35.0 31.0 37.0 2.0 39.0 82-83 29.235758877885452 35.0 30.0 36.0 2.0 38.5 84-85 28.982028734726434 35.0 30.0 36.0 2.0 37.0 86-87 28.679574350380975 35.0 30.0 35.5 2.0 37.0 88-89 28.338467674236846 35.0 29.0 35.0 2.0 36.5 90-91 27.997301495916865 35.0 29.0 35.0 2.0 36.0 92-93 27.658209852917054 35.0 28.0 35.0 2.0 36.0 94-95 27.354618494562786 35.0 27.0 35.0 2.0 36.0 96-97 27.07745896762117 35.0 26.5 35.0 2.0 35.0 98-99 26.812185402347282 35.0 25.0 35.0 2.0 35.0 100 26.619481977273384 35.0 25.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15957901688454967 1101 2 0.1331685956772688 1101 3 0.07809298869648273 1101 4 -0.15224166407861617 1101 5 -0.0394016176059111 1101 6 0.17427045707037792 1101 7 0.07124014872048434 1101 8 -0.23532968251308262 1101 9 -0.0635372997821051 1101 10-11 0.10783996724325817 1101 12-13 -0.09245058928019034 1101 14-15 -0.27165501657067637 1101 16-17 -0.13519364904849596 1101 18-19 -0.22221000651853728 1101 20-21 -0.32753597575584337 1101 22-23 -0.24048956272394406 1101 24-25 -0.006086777521041142 1101 26-27 0.38314122682636764 1101 28-29 0.3867660093875287 1101 30-31 0.4593521294658274 1101 32-33 0.5711711914904356 1101 34-35 0.7638238060653606 1101 36-37 0.6498985716336279 1101 38-39 0.5195163420830227 1101 40-41 0.3532441083271749 1101 42-43 0.3077425222169623 1101 44-45 0.2691184073152968 1101 46-47 0.029164233990293553 1101 48-49 0.0749573684550171 1101 50-51 0.039137561429328116 1101 52-53 -0.0271938395737763 1101 54-55 -0.15694650385359665 1101 56-57 -0.1688333685491159 1101 58-59 -0.24555022367081847 1101 60-61 -0.23495044496094408 1101 62-63 7.591244021298849E-4 1101 64-65 -0.19893186170796895 1101 66-67 -0.21816588136606185 1101 68-69 -0.12939940180345388 1101 70-71 0.02985029405090245 1101 72-73 -0.016022616429424374 1101 74-75 0.110645825129712 1101 76-77 0.02926412779386922 1101 78-79 -0.08641856868587183 1101 80-81 -0.2119201207337582 1101 82-83 -0.022039727192083802 1101 84-85 -0.07410349201655109 1101 86-87 0.06894943842881318 1101 88-89 0.06456313641587386 1101 90-91 -0.001375334396779948 1101 92-93 -0.10457347294286734 1101 94-95 -0.1113232237036037 1101 96-97 0.1587968108768365 1101 98-99 0.14013833041088475 1101 100 -0.11066338128978259 1102 1 0.1805027780107018 1102 2 0.12049247740871039 1102 3 0.4220127861801046 1102 4 0.08030040643778591 1102 5 0.2918169355075264 1102 6 0.09019852104143666 1102 7 -0.055150423966182416 1102 8 0.32499494671987605 1102 9 0.2523166421891645 1102 10-11 0.2654717065348322 1102 12-13 0.2280830891182717 1102 14-15 0.4885948625099559 1102 16-17 0.3764962697313834 1102 18-19 0.48911621194035604 1102 20-21 0.34198983189128995 1102 22-23 0.5246002316817311 1102 24-25 0.39613813877762993 1102 26-27 0.08596540064873182 1102 28-29 -0.026259989846124654 1102 30-31 0.08046308004906422 1102 32-33 -0.02558628509517291 1102 34-35 -0.2065713646979077 1102 36-37 -0.3619609483322037 1102 38-39 -0.34133844789417367 1102 40-41 -0.23530969607868713 1102 42-43 -0.33573065881031283 1102 44-45 -0.21874568560102858 1102 46-47 -0.19054153935970675 1102 48-49 -0.14827234064753725 1102 50-51 -0.33692166079218566 1102 52-53 -0.410589883469072 1102 54-55 -0.3579553168337384 1102 56-57 -0.3389559211097719 1102 58-59 -0.5528832764293554 1102 60-61 -0.3821517113164674 1102 62-63 -0.31127879088521837 1102 64-65 -0.3078095415637492 1102 66-67 -0.4939374097240048 1102 68-69 -0.29555517107046114 1102 70-71 -0.44112277704968506 1102 72-73 -0.1922633747351732 1102 74-75 -0.248889844512302 1102 76-77 -0.4105117473796618 1102 78-79 -0.3693541767689368 1102 80-81 -0.5192123435771556 1102 82-83 -0.3615059231631115 1102 84-85 -0.38790651594288406 1102 86-87 -0.284257594367034 1102 88-89 -0.28073859665698464 1102 90-91 -0.4351931827230686 1102 92-93 -0.5186955983598054 1102 94-95 -0.5489885063408373 1102 96-97 -0.5839433161356098 1102 98-99 -0.6875939761024306 1102 100 -0.5334477302410825 1103 1 -0.12882676292430162 1103 2 -0.22834389104849961 1103 3 -0.13848279672035702 1103 4 -0.06936273699441386 1103 5 -0.12695784665052656 1103 6 -0.24953247578886106 1103 7 -0.644064890117825 1103 8 -0.323625688908578 1103 9 -0.22424199736828143 1103 10-11 -0.40732546844980533 1103 12-13 -0.2786239119543623 1103 14-15 -0.08710144833814581 1103 16-17 -0.2045089970421472 1103 18-19 -0.24717450913757943 1103 20-21 0.013295557850334205 1103 22-23 0.06717130902013935 1103 24-25 0.004908461653748475 1103 26-27 0.09016254911871613 1103 28-29 0.3514863854807828 1103 30-31 0.0999754515434077 1103 32-33 -0.08940523824861657 1103 34-35 -0.35148898850324883 1103 36-37 -0.6060584258248767 1103 38-39 -0.4335883364121287 1103 40-41 -0.42657070869725544 1103 42-43 -0.47706579192590226 1103 44-45 -0.4557052989692352 1103 46-47 -0.5950616844671544 1103 48-49 -0.5715274959390158 1103 50-51 -0.45576453739059986 1103 52-53 -0.45779198535764465 1103 54-55 -0.4233289926077859 1103 56-57 -0.5269929934104027 1103 58-59 -0.47181172303245944 1103 60-61 -0.4577606443428692 1103 62-63 -0.3961045775869181 1103 64-65 -0.5866020403284082 1103 66-67 -0.7401861894475275 1103 68-69 -0.6498029384164674 1103 70-71 -0.7051393848618623 1103 72-73 -0.7525962541055691 1103 74-75 -0.47577389112987944 1103 76-77 -0.5239371990326056 1103 78-79 -0.5487197535228212 1103 80-81 -0.32799953941194815 1103 82-83 -0.4316944182157201 1103 84-85 -0.32209595106033007 1103 86-87 -0.22513293935877599 1103 88-89 -0.34605803792100787 1103 90-91 -0.4024897731802035 1103 92-93 -0.4120434344028574 1103 94-95 -0.5857233477952875 1103 96-97 -0.3610876884012839 1103 98-99 -0.330037903645124 1103 100 -0.2952497622273924 1104 1 0.7568862239263261 1104 2 0.8675203647107423 1104 3 0.5610364613687224 1104 4 0.689573039068506 1104 5 0.6982021165266872 1104 6 0.7678162837151703 1104 7 0.4866446303904226 1104 8 0.842474619084463 1104 9 0.9056549215647678 1104 10-11 0.7802888924362108 1104 12-13 0.897432185966963 1104 14-15 1.051157311279539 1104 16-17 0.7431804868706067 1104 18-19 0.8015844266384846 1104 20-21 0.5667425796371504 1104 22-23 0.5523768460759086 1104 24-25 0.7708337477690606 1104 26-27 0.9862148965712407 1104 28-29 0.9956890699072503 1104 30-31 1.13059859761848 1104 32-33 1.1235787711161933 1104 34-35 1.2287511551510732 1104 36-37 1.3435830066269077 1104 38-39 1.1496749124030643 1104 40-41 1.2363303357514894 1104 42-43 1.323880917321837 1104 44-45 1.3069099142960638 1104 46-47 1.2740952556049052 1104 48-49 1.3780713175490291 1104 50-51 1.3039298562267874 1104 52-53 1.514471593044206 1104 54-55 1.3038670205302196 1104 56-57 1.3328133460183835 1104 58-59 1.3104025310220209 1104 60-61 1.3450840294231625 1104 62-63 1.2938595088329734 1104 64-65 1.1065840397497837 1104 66-67 1.2590466692052544 1104 68-69 1.1231402703937263 1104 70-71 1.1732743941332373 1104 72-73 0.9729035242758215 1104 74-75 0.9531121059833971 1104 76-77 1.0695375534932765 1104 78-79 0.852125806717666 1104 80-81 0.7761441603687089 1104 82-83 0.5868448310086158 1104 84-85 0.705941644548215 1104 86-87 0.3756381797583863 1104 88-89 0.6107007614743694 1104 90-91 0.6818275846713462 1104 92-93 0.48220186898733886 1104 94-95 0.7432268618900153 1104 96-97 0.6952318515412088 1104 98-99 0.5167383979380311 1104 100 0.6689730383499537 1105 1 0.06294267347078275 1105 2 0.16122638547507506 1105 3 -0.017214517186697975 1105 4 0.178755959233996 1105 5 0.09537294832666987 1105 6 0.07944297179296456 1105 7 -0.13684292996777003 1105 8 -0.24661016674850345 1105 9 0.12903877177609502 1105 10-11 0.09375838715591556 1105 12-13 0.1931733789682042 1105 14-15 0.08281678514569535 1105 16-17 0.12711712331635994 1105 18-19 0.22795858608446906 1105 20-21 0.2402201035295164 1105 22-23 0.30791641763033795 1105 24-25 0.008536289225240523 1105 26-27 0.24814713927528942 1105 28-29 0.3191504239380052 1105 30-31 0.35399638736567596 1105 32-33 0.38503149378634305 1105 34-35 0.48634262638545067 1105 36-37 0.24244888598858694 1105 38-39 0.01275800318847331 1105 40-41 0.23669276368408987 1105 42-43 0.04686888471447759 1105 44-45 0.12331507526982222 1105 46-47 0.08437538451772753 1105 48-49 0.05034167763512443 1105 50-51 0.06548015564801801 1105 52-53 0.17216398651827092 1105 54-55 0.13707489804072992 1105 56-57 0.18883466793245418 1105 58-59 0.12403930017215714 1105 60-61 0.24097242392318208 1105 62-63 0.18589780352591845 1105 64-65 0.2362858451984735 1105 66-67 0.3810315475967414 1105 68-69 0.2119128106232253 1105 70-71 0.24399802331927845 1105 72-73 0.2117881383737057 1105 74-75 0.2898662014190343 1105 76-77 0.28147983266314824 1105 78-79 0.3405978105538239 1105 80-81 0.19293628285819864 1105 82-83 0.15237458912978852 1105 84-85 0.504532078681116 1105 86-87 0.33894932135061495 1105 88-89 0.09045497957792392 1105 90-91 0.35258132325486713 1105 92-93 0.49235263900730075 1105 94-95 0.4912753268683261 1105 96-97 0.5201206140449735 1105 98-99 0.5513725687586764 1105 100 0.5596034399201031 1106 1 0.04296137062819838 1106 2 -0.2093831213103492 1106 3 0.004775189103561672 1106 4 0.06773901878752753 1106 5 -0.2077007821671586 1106 6 -0.1993141808970691 1106 7 -0.18154146398735094 1106 8 -0.20750537020850857 1106 9 -0.19376970563835982 1106 10-11 -0.12983444628527963 1106 12-13 -0.10454289680459539 1106 14-15 -0.057144793223137924 1106 16-17 -0.08918176410004008 1106 18-19 -0.10306585377129807 1106 20-21 0.06694008791430406 1106 22-23 0.2083746007186562 1106 24-25 0.2169633651747347 1106 26-27 0.26870630755998093 1106 28-29 0.14299905914824507 1106 30-31 0.14177080924340402 1106 32-33 0.0969437404244502 1106 34-35 0.09633225049040561 1106 36-37 0.02608065471913079 1106 38-39 -0.06772452729713763 1106 40-41 0.08558993959146477 1106 42-43 0.16753232560783005 1106 44-45 -0.053532296341323615 1106 46-47 0.03709571819007351 1106 48-49 0.08198302095397025 1106 50-51 -0.08857106634476963 1106 52-53 -0.07551941513159477 1106 54-55 -0.18275179369245365 1106 56-57 0.09493281773572448 1106 58-59 0.09505613788699208 1106 60-61 0.03341856686397193 1106 62-63 0.02878261054453901 1106 64-65 -0.002778372346035951 1106 66-67 0.21670973047960018 1106 68-69 0.08393487737824046 1106 70-71 0.3299998108497171 1106 72-73 -0.1351776828116087 1106 74-75 -0.18410417077767605 1106 76-77 -0.14409623497535762 1106 78-79 -0.10051898504104173 1106 80-81 -0.1847206140300628 1106 82-83 -0.15715325627964916 1106 84-85 -0.16137437389282283 1106 86-87 -0.046751462491751994 1106 88-89 -0.2807606438489998 1106 90-91 -0.1870217217107708 1106 92-93 -0.09868556688743979 1106 94-95 0.05069787235275669 1106 96-97 0.07050277572401953 1106 98-99 0.05706646958155659 1106 100 0.05090207050741924 1107 1 0.5396467799662972 1107 2 0.5094167937474943 1107 3 0.49996026144288663 1107 4 0.5675358962399955 1107 5 0.3392666905267063 1107 6 0.3285366902216751 1107 7 0.5912428844970776 1107 8 0.5364171108410858 1107 9 0.40968891737542634 1107 10-11 0.23451431512881982 1107 12-13 0.47327381279479397 1107 14-15 0.4607807715960348 1107 16-17 0.3427675660571978 1107 18-19 0.313683908482993 1107 20-21 0.40715419754508275 1107 22-23 0.40979138048764696 1107 24-25 0.42435610262037926 1107 26-27 0.5619137049138487 1107 28-29 0.5077609902896967 1107 30-31 0.5134573726868865 1107 32-33 0.7212916708826782 1107 34-35 0.4130972469357559 1107 36-37 0.47779981009865224 1107 38-39 0.5927930658853242 1107 40-41 0.5467450605878739 1107 42-43 0.5479831653349123 1107 44-45 0.5857266756739321 1107 46-47 0.5928736313828864 1107 48-49 0.6128899631286799 1107 50-51 0.6831630462405087 1107 52-53 0.6572474832794271 1107 54-55 0.7668068764088289 1107 56-57 0.7116105638140766 1107 58-59 0.5541961908835162 1107 60-61 0.6522014011621451 1107 62-63 0.5427796006377008 1107 64-65 0.4426209019525018 1107 66-67 0.5878123165989422 1107 68-69 0.4876155502678472 1107 70-71 0.6636955332735184 1107 72-73 0.6868431095509848 1107 74-75 0.42641757718417495 1107 76-77 0.5114079244204426 1107 78-79 0.6747928542336226 1107 80-81 0.4366220260090863 1107 82-83 0.5571110246203652 1107 84-85 0.7517538056675086 1107 86-87 0.6115617894079044 1107 88-89 0.7250006502262885 1107 90-91 0.5619026520716446 1107 92-93 0.21962131435050125 1107 94-95 0.38813751264119034 1107 96-97 0.30085990209394 1107 98-99 0.3500592033340624 1107 100 0.4181938006498811 1108 1 -0.1208857874910656 1108 2 0.05934056072263871 1108 3 -0.05384607121676055 1108 4 0.05997464389096052 1108 5 0.3161485825240149 1108 6 0.17387229281714411 1108 7 0.07348768114991344 1108 8 -0.2425071170779347 1108 9 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-0.7505355662608437 2215 32-33 -0.8264995975234228 2215 34-35 -1.0213942711904807 2215 36-37 -0.956207337064015 2215 38-39 -0.9683668411154471 2215 40-41 -0.8560901241189214 2215 42-43 -0.8709116077642065 2215 44-45 -1.1003363571341254 2215 46-47 -0.9864663324507461 2215 48-49 -1.0313987650208105 2215 50-51 -0.7075628643768965 2215 52-53 -0.8543881836455682 2215 54-55 -0.8471279380232701 2215 56-57 -0.7433537003726087 2215 58-59 -0.8048195673185035 2215 60-61 -0.8582179564543253 2215 62-63 -0.8492056744850061 2215 64-65 -0.818842544185447 2215 66-67 -1.0340661816357937 2215 68-69 -0.9130991659984105 2215 70-71 -0.8986914468349774 2215 72-73 -0.9280389192098895 2215 74-75 -1.0472756975940918 2215 76-77 -1.2175380843628183 2215 78-79 -1.1615220514754938 2215 80-81 -0.9912282280165741 2215 82-83 -0.9822306614211378 2215 84-85 -0.8262622108656892 2215 86-87 -0.7978716042027045 2215 88-89 -0.7467228493001095 2215 90-91 -0.9619606907338287 2215 92-93 -1.0403674912983405 2215 94-95 -1.04889426209402 2215 96-97 -1.306218372253774 2215 98-99 -1.2057295678706232 2215 100 -1.2802691288257044 2216 1 -0.07396701998406385 2216 2 -0.32441294067792015 2216 3 -0.33699266318688004 2216 4 -0.05393758766833656 2216 5 -0.25598026901660376 2216 6 -0.34106701623981195 2216 7 0.025635206826855494 2216 8 -0.1792484802898855 2216 9 -0.299295942391268 2216 10-11 -0.31407094175874306 2216 12-13 -0.344143660523649 2216 14-15 -0.5581548617290224 2216 16-17 -0.3942742971015676 2216 18-19 -0.34012803823662097 2216 20-21 -0.40705735205365556 2216 22-23 -0.43055361827134675 2216 24-25 -0.3656352103605087 2216 26-27 -0.28310573746226453 2216 28-29 -0.4643578150383334 2216 30-31 -0.4122169054631897 2216 32-33 -0.4392113488906375 2216 34-35 -0.48246203162798906 2216 36-37 -0.4581747907121425 2216 38-39 -0.45322745202647496 2216 40-41 -0.3929215233452794 2216 42-43 -0.2580998163809838 2216 44-45 -0.39697167792910903 2216 46-47 -0.37909004860387796 2216 48-49 -0.41165086586114796 2216 50-51 -0.5217885546530141 2216 52-53 -0.54458092740974 2216 54-55 -0.5785538417180831 2216 56-57 -0.7343334255960343 2216 58-59 -0.6234636916348961 2216 60-61 -0.8106822755152763 2216 62-63 -0.6152620970540355 2216 64-65 -0.5259920706627028 2216 66-67 -0.41973711667647606 2216 68-69 -0.6122955112031576 2216 70-71 -0.680320765229002 2216 72-73 -0.896676374191884 2216 74-75 -0.8917134727041365 2216 76-77 -0.9806599997956553 2216 78-79 -1.0017914925852693 2216 80-81 -0.9509121015659936 2216 82-83 -0.847176639812492 2216 84-85 -0.8768657856289259 2216 86-87 -0.7747623605052247 2216 88-89 -1.0057431240766839 2216 90-91 -0.9147251298428074 2216 92-93 -1.0846518717171776 2216 94-95 -1.0406909807814948 2216 96-97 -0.9698338184322424 2216 98-99 -1.096319137031621 2216 100 -1.2130422380694021 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9402.0 3 1067.0 4 802.0 5 888.0 6 2416.0 7 6100.0 8 6740.0 9 3885.0 10 2105.0 11 1995.0 12 1825.0 13 1318.0 14 1050.0 15 857.0 16 851.0 17 859.0 18 770.0 19 685.0 20 650.0 21 685.0 22 765.0 23 1289.0 24 2523.0 25 2264.0 26 2542.0 27 2025.0 28 2139.0 29 2653.0 30 3218.0 31 3998.0 32 5303.0 33 7041.0 34 9515.0 35 15924.0 36 21983.0 37 48811.0 38 90990.0 39 42926.0 40 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.10193887272447 23.15253495378119 13.379959832118857 20.365566341375494 2 15.363916971634014 27.345995513198083 36.06822256339941 21.221864951768488 3 18.655371304974764 32.25969461324544 28.693094036461016 20.391840045318776 4 12.05476982895694 22.984273308398944 35.97923278464797 28.98172407799615 5 11.293037687447494 43.804214531023185 31.722923714220418 13.179824067308898 6 25.569793004445046 42.66696278843709 16.465014178439116 15.29823002867875 7 22.206092988074737 40.54685614046382 20.512737982200623 16.734312889260828 8 21.352242149579638 40.34903631986198 19.633453927463272 18.665267603095106 9 22.27561678227143 24.214880023174597 19.898611133463152 33.610892061090816 10-11 20.161739382332588 33.777459484687064 25.91724737274088 20.14355376023947 12-13 22.08966609374095 32.79339912323053 25.44546584010866 19.671468942919862 14-15 20.439505616530948 34.32151018700312 24.168785606231292 21.07019859023464 16-17 18.42125167567602 37.062010875600066 25.232904885973568 19.283832562750344 18-19 18.540118733233236 35.91157286934356 26.873814156258604 18.67449424116461 20-21 21.062803953691215 34.48713698555184 25.51714371236285 18.932915348394094 22-23 20.337471937687585 34.69459352897157 25.304923678556772 19.663010854784073 24-25 20.375639288438574 34.792740193822276 25.06734857433448 19.76427194340467 26-27 19.659702799172187 34.21285552898464 26.10146798026386 20.02597369157931 28-29 19.39551544683001 35.37660398247889 26.34634757057201 18.881533000119084 30-31 20.147314179593266 35.89049120282977 25.296712386525115 18.665482231051847 32-33 18.753882349911972 35.79920888571328 26.318872485122352 19.1280362792524 34-35 18.64035017138443 35.46531275647318 26.11480343090713 19.779533641235254 36-37 19.12916701532599 35.66929716716981 25.69839144679673 19.503144370707467 38-39 19.118186616545223 35.10807435824815 26.332745436288267 19.440993588918356 40-41 18.372046616865624 35.05743427002636 26.776053015258945 19.79446609784907 42-43 18.381124654399386 34.934388186333045 26.632248761631335 20.052238397636238 44-45 18.454878294105814 34.90661838664252 26.7981492657413 19.84035405351036 46-47 18.398237394846408 35.133169068146444 26.47689799518148 19.99169554182566 48-49 18.02876065246606 34.305809265533085 26.489896754568793 21.17553332743206 50-51 17.690570726392213 33.820620571 26.56072206279745 21.928086639810342 52-53 18.07893055392375 33.454632072116155 26.19634301201775 22.27009436194235 54-55 18.08517829437402 33.330972813246376 26.139809312895522 22.444039579484077 56-57 18.705590577919978 32.20347984787937 25.902190234473704 23.18873933972695 58-59 18.884364689788363 31.614066146294363 26.051017944797618 23.45055121911966 60-61 18.896170360690352 31.563463605873533 25.757687925274077 23.782678108162045 62-63 18.477634615570313 31.036497243152944 26.066769451624012 24.419098689652728 64-65 18.30908848043594 31.143169356694656 26.20954698415917 24.338195178710233 66-67 18.51006646603473 30.740621529845342 26.044224857975117 24.70508714614481 68-69 18.815751569508173 31.04801785722907 26.30432928369916 23.831901289563596 70-71 18.961743709244466 30.53760291476501 26.60334605753571 23.897307318454814 72-73 18.57635759713675 30.00215644874733 27.803061513505163 23.61842444061076 74-75 18.77393834448042 30.05867509510966 27.568282618911212 23.599103941498708 76-77 19.052477594550275 29.61719631342042 28.039440672429027 23.290885419600283 78-79 19.74763071882277 29.115967241237804 28.868458024013865 22.26794401592556 80-81 20.23695530775705 29.18779561075858 28.398266447585467 22.176982633898902 82-83 19.839262034426376 28.988676889009053 28.841907741428813 22.330153335135762 84-85 20.10190074172539 29.03012444700502 28.121193004703976 22.746781806565615 86-87 20.301843310556148 28.84521920493352 27.958003289441923 22.894934195068412 88-89 20.69044871763924 28.49774619282805 27.705493946664994 23.106311142867717 90-91 20.892265455651447 28.190702711749303 27.597532695962553 23.319499136636697 92-93 21.164058627770306 28.774951240079044 27.194518290602694 22.86647184154796 94-95 20.56825697728871 28.897231935810392 27.204144130516294 23.330366956384605 96-97 19.889702805867927 29.422830585827114 27.19033427098286 23.4971323373221 98-99 20.031669606189734 30.24994528624947 26.361245928653272 23.357139178907527 100 19.569373370667183 30.878954652248076 26.236040037333847 23.315631939750894 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6284.0 1 3665.5 2 920.0 3 736.5 4 615.5 5 420.0 6 289.0 7 305.5 8 352.5 9 375.5 10 288.0 11 188.0 12 160.0 13 155.0 14 159.0 15 151.0 16 168.0 17 209.5 18 245.5 19 293.5 20 372.0 21 488.5 22 701.0 23 989.5 24 1251.5 25 1601.5 26 2122.5 27 2709.0 28 3611.5 29 4563.0 30 5193.0 31 5949.0 32 7055.5 33 8360.5 34 9504.5 35 10398.5 36 11184.5 37 11889.0 38 11998.0 39 11671.0 40 11787.5 41 11533.5 42 10560.5 43 9862.5 44 9677.5 45 9495.0 46 9456.0 47 10374.5 48 12524.5 49 12829.5 50 11672.5 51 10598.0 52 8903.0 53 7610.5 54 6512.5 55 5763.5 56 5119.5 57 4439.5 58 3712.5 59 2945.0 60 2303.5 61 1787.0 62 1411.5 63 1083.5 64 864.5 65 712.0 66 556.0 67 460.5 68 371.5 69 274.0 70 232.5 71 203.5 72 164.0 73 126.5 74 111.5 75 102.0 76 70.0 77 56.5 78 48.0 79 30.5 80 25.5 81 21.5 82 19.0 83 15.0 84 11.5 85 8.5 86 4.5 87 1.5 88 2.5 89 2.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06979444410494254 2 0.07204587778574714 3 0.07236751116871923 4 0.07429731146655173 5 0.07268914455169131 6 0.07397567808357965 7 0.07333241131763549 8 0.07365404470060756 9 0.07333241131763549 10-11 0.07333241131763549 12-13 0.07301077793466339 14-15 0.07172424440277506 16-17 0.06995526079642858 18-19 0.0696336274134565 20-21 0.06947281072197045 22-23 0.07092016094534484 24-25 0.06947281072197045 26-27 0.06882954395602629 28-29 0.06496994336036126 30-31 0.0696336274134565 32-33 0.0681862771900821 34-35 0.06689974365819377 36-37 0.064005043211445 38-39 0.06464830997738917 40-41 0.06754301042413793 42-43 0.07140261101980297 44-45 0.07252832786020526 46-47 0.07606629507289821 48-49 0.07896099551964697 50-51 0.07960426228559114 52-53 0.07960426228559114 54-55 0.07944344559410511 56-57 0.07670956183884238 58-59 0.07654874514735634 60-61 0.07429731146655173 62-63 0.07461894484952382 64-65 0.07349322800912152 66-67 0.07333241131763549 68-69 0.07284996124317736 70-71 0.07043771087088671 72-73 0.06979444410494254 74-75 0.07043771087088671 76-77 0.07027689417940067 78-79 0.06995526079642858 80-81 0.07124179432831693 82-83 0.07108097763683088 84-85 0.07027689417940067 86-87 0.07140261101980297 88-89 0.0696336274134565 90-91 0.0673821937326519 92-93 0.06657811027522169 94-95 0.06706056034967982 96-97 0.06657811027522169 98-99 0.06593484350927752 100 0.06529157674333334 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 310913.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.74138964792547 #Duplication Level Percentage of deduplicated Percentage of total 1 85.77599637668483 60.679131821221056 2 10.093724777994764 14.280882350380953 3 2.242711894572498 4.759576680059708 4 0.6981544052233416 1.9755365125728026 5 0.29483436681561465 1.0428496412251393 6 0.1564582783730061 0.6640845620417051 7 0.11505261331232279 0.5697287223837387 8 0.0659287395618998 0.3731112523475963 9 0.05774373101456575 0.36763845978837556 >10 0.42078425376161416 5.944302757205853 >50 0.0468028205584336 2.214121897559052 >100 0.029990156862680535 3.9827192669502036 >500 9.087926322024404E-4 0.40630808340799496 >1k 4.543963161012202E-4 0.3690203162597929 >5k 4.543963161012202E-4 2.3709876765960214 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7376 2.372367832802102 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1148 0.3692351236519541 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 720 0.23157603573990154 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 544 0.17496856033681446 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 435 0.13991052159285716 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 412 0.1325129537844992 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 394 0.12672355289100168 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 370 0.1190043516996716 No Hit GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 346 0.11128515050834156 No Hit GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 327 0.10517411623187194 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 322 0.10356594931701152 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 321 0.10324431593403943 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.6081669148604273E-4 0.0 14-15 0.0 0.0 0.0 6.432667659441709E-4 0.0 16-17 0.0 0.0 0.0 0.001125716840402299 0.0 18-19 0.0 0.0 0.0 0.0014473502233743846 0.0 20-21 0.0 0.0 0.0 0.0019298002978325127 0.0 22-23 0.0 0.0 0.0 0.005628584202011495 0.0 24-25 0.0 0.0 0.0 0.018815552903866998 0.0 26-27 0.0 0.0 0.0 0.049531540977701155 0.0 28-29 0.0 0.0 0.0 0.12768845303991794 0.0 30-31 0.0 0.0 0.0 0.28705779430258627 0.0 32-33 0.0 0.0 0.0 0.538253466403785 0.0 34-35 0.0 0.0 0.0 0.94849684638468 0.0 36-37 0.0 0.0 0.0 1.5044401488519297 0.0 38-39 0.0 0.0 0.0 2.14529466442381 0.0 40-41 0.0 0.0 0.0 2.8112365838675126 0.0 42-43 0.0 0.0 0.0 3.335498998112012 0.0 44-45 0.0 0.0 0.0 3.8464136269631695 0.0 46-47 0.0 0.0 0.0 4.421815749100231 0.0 48-49 0.0 0.0 0.0 5.026968959162209 0.0 50-51 0.0 0.0 0.0 5.560237108129927 0.0 52-53 0.0 0.0 0.0 6.072438270512974 0.0 54-55 0.0 0.0 0.0 6.639477924692759 0.0 56-57 0.0 0.0 0.0 7.271326705541421 0.0 58-59 0.0 0.0 0.0 7.865061930507892 0.0 60-61 0.0 0.0 0.0 8.433388118219566 0.0 62-63 0.0 0.0 0.0 8.987401620388983 0.0 64-65 0.0 0.0 0.0 9.574382544313039 0.0 66-67 0.0 0.0 0.0 10.274739235734756 0.0 68-69 0.0 0.0 0.0 11.037975253527513 0.0 70-71 0.0 0.0 0.0 11.827102758649525 0.0 72-73 0.0 0.0 0.0 12.620893947824634 0.0 74-75 0.0 0.0 0.0 13.420474537893238 0.0 76-77 0.0 0.0 0.0 14.251092749418648 0.0 78-79 0.0 0.0 0.0 15.17675362561231 0.0 80-81 0.0 0.0 0.0 16.10981206961433 0.0 82-83 0.0 0.0 0.0 17.032899878744214 0.0 84-85 0.0 0.0 0.0 17.97432079070351 0.0 86-87 0.0 0.0 0.0 18.962378543193758 0.0 88 0.0 3.2163338297208546E-4 0.0 19.73027824503961 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTTGCG 25 0.0052301814 56.406185 7 TGCACGA 55 0.002712511 34.185566 5 GAGAACA 430 0.0 32.794296 1 TAGACCA 60 0.0041541196 31.336767 5 CGATGCT 60 0.0041541196 31.336767 9 CCGTATC 140 1.8824721E-8 30.212732 94 GCGCCGG 125 2.3548637E-7 30.078457 94 TACATGA 220 0.0 29.91237 2 ATGCACG 65 0.0061440566 28.926247 4 CTATAAC 90 9.4959117E-4 26.113974 3 ATGGGAC 110 1.01523634E-4 25.639174 3 GTACCCA 110 1.01523634E-4 25.639174 1 TAAACAG 220 2.4301698E-9 23.502577 5 TAACAAC 180 2.148081E-7 23.502577 1 CATGGGG 605 0.0 23.30834 4 CAACACA 1000 0.0 23.032524 6 TACCACA 225 3.1523086E-9 22.980297 2 TGGACAT 105 0.0023302045 22.383406 5 AGGACAG 105 0.0023302045 22.383406 5 GTGTAAG 105 0.0023302045 22.383406 1 >>END_MODULE