FastQCFastQC Report
Wed 25 May 2016
SRR1032987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310913
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41581.3373516063979314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37791.2154525542515109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22650.7284996124317735No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT20690.6654594693692448No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12170.39142782707702795No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11940.38403025926867No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10750.34575588669499185No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9680.31134111471697873No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9410.3026570133767324No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT8580.2759614425900493TruSeq Adapter, Index 1 (95% over 21bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7080.22771643514423648No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6820.2193539671869623No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5300.17046569297520528No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT5000.16081669148604272RNA PCR Primer, Index 1 (95% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4710.15148932337985224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4000.1286533531888342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3960.12736681965694582No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3870.12447211921019707No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.11450148433806243No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3510.11289331742320198No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3490.11225005065725782No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3470.11160678389131365No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3430.1103202503594253No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3250.10453084946592778No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.10002798210431857No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGAC550.00270671134.2004975
GGCTTAG550.002717414234.1729851
CGGTACT702.1846642E-433.562751
ATGGGCA1755.487891E-926.8718175
GGTATCA44100.025.2519741
CTCTAGC950.001295868324.7583312
CGGAACA1151.3794762E-424.50751594
CACGATG1000.001750230723.512847
GTATCAA58300.023.3730321
ACTGTAC1050.002324301322.3931838
GAGTACT41000.022.22612812-13
TAGATGT1303.1374354E-421.7076534
TTATATG1100.003039227821.3821962
TATCAAC64550.020.6965072
CAACGCA65250.020.6840925
ATCAACG64900.020.5815773
GTCCAGG1605.4073396E-520.5571861
TCAACGC65550.020.449244
TAGCCTC1150.00393975320.4459485
GTACCCA1150.003958163320.42951
Produced by FastQC (version 0.11.5)