##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032987_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 310913 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70469230942418 34.0 31.0 34.0 31.0 34.0 2 32.72399674507017 34.0 31.0 34.0 31.0 34.0 3 32.75879747710774 34.0 31.0 34.0 31.0 34.0 4 36.244553942742826 37.0 35.0 37.0 35.0 37.0 5 36.25026615162441 37.0 37.0 37.0 35.0 37.0 6 36.347180722581555 37.0 37.0 37.0 35.0 37.0 7 36.30499207173711 37.0 37.0 37.0 35.0 37.0 8 36.26102478828483 37.0 37.0 37.0 35.0 37.0 9 38.0664140772499 39.0 39.0 39.0 35.0 39.0 10-11 38.09065236899069 39.0 39.0 39.0 35.0 39.0 12-13 38.081408625564066 39.0 39.0 39.0 35.0 39.0 14-15 39.468251568766824 41.0 39.0 41.0 36.5 41.0 16-17 39.48533030140264 41.0 39.0 41.0 36.5 41.0 18-19 39.59346987742552 41.0 40.0 41.0 37.0 41.0 20-21 39.61578158520229 41.0 40.0 41.0 37.0 41.0 22-23 39.509095792070454 41.0 40.0 41.0 36.0 41.0 24-25 39.43034868275048 41.0 39.5 41.0 36.0 41.0 26-27 39.24912756944869 41.0 39.0 41.0 35.0 41.0 28-29 39.10839045006159 41.0 39.0 41.0 35.0 41.0 30-31 38.7663783759444 41.0 38.5 41.0 35.0 41.0 32-33 38.34175637557773 41.0 37.0 41.0 35.0 41.0 34-35 38.087090279274264 40.0 37.0 41.0 35.0 41.0 36-37 37.84303808460888 40.0 37.0 41.0 34.0 41.0 38-39 37.55158517012798 40.0 36.5 41.0 33.5 41.0 40-41 37.32455059775564 40.0 37.0 41.0 33.0 41.0 42-43 36.97497853097168 40.0 36.0 41.0 33.0 41.0 44-45 36.46334022700884 40.0 36.0 41.0 31.0 41.0 46-47 35.73727377111925 40.0 36.0 41.0 25.5 41.0 48-49 35.10680318931662 40.0 35.0 41.0 8.5 41.0 50-51 34.752030310730014 40.0 35.0 41.0 2.0 41.0 52-53 34.56932485936581 40.0 35.0 41.0 2.0 41.0 54-55 34.39788783357402 40.0 35.0 41.0 2.0 41.0 56-57 34.18756693994783 40.0 35.0 41.0 2.0 41.0 58-59 34.03903664369132 40.0 35.0 41.0 2.0 41.0 60-61 33.80766806148344 39.0 35.0 41.0 2.0 41.0 62-63 33.56098973024608 39.0 35.0 41.0 2.0 41.0 64-65 33.24445102006027 38.5 35.0 41.0 2.0 41.0 66-67 32.86319806505357 37.5 34.5 40.5 2.0 41.0 68-69 32.44772653443246 37.0 34.0 40.0 2.0 41.0 70-71 31.9348853216173 36.5 34.0 39.5 2.0 41.0 72-73 31.507746540028883 36.0 33.5 39.0 2.0 41.0 74-75 31.167743066388347 35.5 33.0 39.0 2.0 41.0 76-77 30.784549053915406 35.0 33.0 37.5 2.0 40.0 78-79 30.422077880307352 35.0 32.5 37.0 2.0 39.0 80-81 30.089269345443903 35.0 32.0 36.5 2.0 39.0 82-83 29.777715952694162 35.0 32.0 36.0 2.0 38.0 84-85 29.506611174186993 35.0 32.0 36.0 2.0 37.0 86-87 29.368340982847293 35.0 32.0 35.5 2.0 37.0 88-89 29.17113308224487 35.0 32.0 35.0 2.0 36.5 90-91 28.980840299376354 35.0 32.0 35.0 2.0 36.0 92-93 28.81826588145237 35.0 31.0 35.0 2.0 36.0 94-95 28.70621202715872 35.0 31.5 35.0 2.0 36.0 96-97 28.64679347598846 35.0 32.0 35.0 2.0 35.5 98-99 28.53205719928083 35.0 31.5 35.0 2.0 35.0 100 28.434812954106132 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1426101259090089 1101 2 0.16301307578186908 1101 3 0.08782062372344512 1101 4 0.13442672503269648 1101 5 0.0745001992624239 1101 6 0.12339358452695848 1101 7 0.06300473443260302 1101 8 -0.0833340946886878 1101 9 -0.0535038020607459 1101 10-11 0.07047941360399079 1101 12-13 0.12127606264379409 1101 14-15 0.25232800181400705 1101 16-17 0.23227232711196422 1101 18-19 0.1443161201851808 1101 20-21 0.2611416363640444 1101 22-23 0.27581410676296514 1101 24-25 0.30296206515431123 1101 26-27 0.3046889626749518 1101 28-29 0.18762745505945588 1101 30-31 0.04266062976503804 1101 32-33 0.2209605374196002 1101 34-35 0.21106680269137712 1101 36-37 0.5305032426712799 1101 38-39 0.4239310483346941 1101 40-41 0.27077503502525957 1101 42-43 0.17294872745282674 1101 44-45 -0.06602013861716927 1101 46-47 -0.11664156146829896 1101 48-49 0.10235707398653204 1101 50-51 0.017364493540945602 1101 52-53 -0.1920874665829615 1101 54-55 -0.22335116488952877 1101 56-57 -0.183895902362039 1101 58-59 -0.047674134192234874 1101 60-61 0.08899787705049533 1101 62-63 -0.13449352027737405 1101 64-65 -0.07083074687167823 1101 66-67 -0.07414531722834283 1101 68-69 -0.1114663662950619 1101 70-71 0.08427121604487553 1101 72-73 0.05887655163778405 1101 74-75 0.029641796745714544 1101 76-77 0.0986066588378236 1101 78-79 0.10249729862134771 1101 80-81 0.23672316710375085 1101 82-83 0.2609230965899094 1101 84-85 0.2584567394393389 1101 86-87 0.30668795389972914 1101 88-89 0.30009102515672126 1101 90-91 0.17637440236893198 1101 92-93 0.16814426357500878 1101 94-95 0.3814169763823827 1101 96-97 0.037233733445564354 1101 98-99 -0.09092926419614145 1101 100 -0.2073702625902989 1102 1 0.2698172968984096 1102 2 0.2680527436148026 1102 3 0.23522918041734897 1102 4 0.23912719049373266 1102 5 0.12088926438299552 1102 6 0.12587478706218036 1102 7 0.18289392832444662 1102 8 0.041976055628211384 1102 9 0.09294368124609775 1102 10-11 0.20970628345705933 1102 12-13 0.29111908732391356 1102 14-15 0.2071198634696998 1102 16-17 0.19726538602639465 1102 18-19 0.15871651717758084 1102 20-21 0.04512311842715633 1102 22-23 0.1847853813472824 1102 24-25 0.14093953960420436 1102 26-27 0.2768523828398983 1102 28-29 0.14358768044058934 1102 30-31 0.15111430665593417 1102 32-33 0.16332440106024393 1102 34-35 0.03710309734517381 1102 36-37 0.08420714412670804 1102 38-39 0.17131008185918972 1102 40-41 -0.038586950439288614 1102 42-43 -0.07763702479125101 1102 44-45 0.18346320591821552 1102 46-47 0.07224390747897758 1102 48-49 0.17585092123239576 1102 50-51 0.25154918295092443 1102 52-53 0.37053306018576393 1102 54-55 0.4277006765396436 1102 56-57 0.256743682554756 1102 58-59 0.13704668341111415 1102 60-61 0.11016975843016752 1102 62-63 0.18161482347282032 1102 64-65 0.3928023617924339 1102 66-67 0.15259576457597035 1102 68-69 0.05747840025203743 1102 70-71 0.13426901751099507 1102 72-73 0.17620288316413024 1102 74-75 0.3258942386887149 1102 76-77 0.3779021229483277 1102 78-79 0.25102082607475396 1102 80-81 0.15018887311580187 1102 82-83 0.394169954256828 1102 84-85 0.31111790767743486 1102 86-87 0.33563636373157024 1102 88-89 0.21734449098835995 1102 90-91 0.18995820105860517 1102 92-93 0.06668192446058185 1102 94-95 -0.011307132740277837 1102 96-97 0.028310826606865902 1102 98-99 0.10170313465559389 1102 100 0.1076105473495872 1103 1 0.1931357257849129 1103 2 0.18758680891315294 1103 3 0.2386218503220192 1103 4 0.18070457108741778 1103 5 0.1414381176244106 1103 6 0.12952657740361673 1103 7 0.2285955421024255 1103 8 0.11719329497113051 1103 9 0.24172702040462468 1103 10-11 0.2444374610690616 1103 12-13 0.3068199779750813 1103 14-15 0.43780032578876416 1103 16-17 0.3777638614340546 1103 18-19 0.4297896373492094 1103 20-21 0.39057284303636663 1103 22-23 0.37201797508259205 1103 24-25 0.41778863509309616 1103 26-27 0.40658434383926334 1103 28-29 0.6004411146902839 1103 30-31 0.3892791313579451 1103 32-33 0.29122745386055016 1103 34-35 0.0852181468190949 1103 36-37 -0.021498928933198158 1103 38-39 0.029456707870068044 1103 40-41 -0.14167470187650366 1103 42-43 -0.05629574509786295 1103 44-45 0.05194451911648912 1103 46-47 0.0072890426434213396 1103 48-49 -0.2301847771273131 1103 50-51 -0.49639894475327395 1103 52-53 -0.3605240306751156 1103 54-55 -0.4867775272133841 1103 56-57 -0.5662981503306384 1103 58-59 -0.6359999703196166 1103 60-61 -0.6492730723702138 1103 62-63 -0.7432944843743101 1103 64-65 -0.6187134325020978 1103 66-67 -0.33358046142525666 1103 68-69 -0.23031100111948888 1103 70-71 -0.18961345027973664 1103 72-73 -0.4182977375197048 1103 74-75 -0.3213795732742142 1103 76-77 -0.25543944599578694 1103 78-79 -0.28260155711013724 1103 80-81 -0.2632649010034953 1103 82-83 -0.3208849431194274 1103 84-85 -0.3371833469475618 1103 86-87 -0.32302066927916684 1103 88-89 -0.04353555432758682 1103 90-91 -0.17720039270081145 1103 92-93 -0.20250805300197072 1103 94-95 -0.27887308793052057 1103 96-97 -0.24283761927312142 1103 98-99 -0.29522116602052506 1103 100 -0.26586636682731424 1104 1 0.09152643371431424 1104 2 0.10480941628453166 1104 3 0.1148847922806695 1104 4 0.12770362778768884 1104 5 0.06005294447776777 1104 6 -0.05457598087071602 1104 7 0.08680509102330802 1104 8 0.026545600372948286 1104 9 0.1277354987371382 1104 10-11 0.12899405337036995 1104 12-13 0.23733686076725036 1104 14-15 0.33959202702414615 1104 16-17 0.2049297571710227 1104 18-19 0.13461353579106117 1104 20-21 0.13001754635155294 1104 22-23 0.1527547717276292 1104 24-25 0.19040793977891468 1104 26-27 0.2518519341062344 1104 28-29 0.0781712855070893 1104 30-31 0.15482084679964458 1104 32-33 0.35138270053911214 1104 34-35 0.28901919013132726 1104 36-37 0.3901178640750089 1104 38-39 0.518830379579029 1104 40-41 0.5690732325243886 1104 42-43 0.6109836875584946 1104 44-45 0.7719265623264278 1104 46-47 0.8264993984833637 1104 48-49 1.0218694569246694 1104 50-51 1.043833357011728 1104 52-53 1.175366819317496 1104 54-55 1.1903355210085564 1104 56-57 1.1601193564031576 1104 58-59 1.1015638160627788 1104 60-61 1.0592444695952636 1104 62-63 1.0615277093089972 1104 64-65 1.2669086338925695 1104 66-67 1.0753058766397245 1104 68-69 1.1442216109115577 1104 70-71 1.1585096105574095 1104 72-73 0.9605564374339792 1104 74-75 1.0686890716700432 1104 76-77 1.070337071523447 1104 78-79 1.2573210468517217 1104 80-81 1.2456793988521717 1104 82-83 1.1789871999893684 1104 84-85 1.1823702336136463 1104 86-87 1.0597459265453146 1104 88-89 0.9871500995609672 1104 90-91 1.0030636175732006 1104 92-93 0.8757517294708421 1104 94-95 0.8186078471863709 1104 96-97 0.8477134723406046 1104 98-99 0.7715996865860504 1104 100 0.6946798378119041 1105 1 0.09593690286333612 1105 2 0.14627674758826714 1105 3 0.04085470518645451 1105 4 0.14431397872944274 1105 5 0.19221647196096114 1105 6 0.047322026333240785 1105 7 0.08419003409424874 1105 8 0.03479780560751777 1105 9 0.2683909914718541 1105 10-11 0.059137463315387606 1105 12-13 0.17221971290780402 1105 14-15 0.27679441793333837 1105 16-17 0.18323705145134994 1105 18-19 0.31308141352459984 1105 20-21 0.45039268714086234 1105 22-23 0.376729658259805 1105 24-25 0.3056460828097016 1105 26-27 0.37087326717192326 1105 28-29 0.3308755554657168 1105 30-31 0.26748296360180746 1105 32-33 0.2130606861089035 1105 34-35 0.33905376018028477 1105 36-37 0.2937008823748357 1105 38-39 0.2835338020138778 1105 40-41 0.11990514402506847 1105 42-43 0.03478182077585501 1105 44-45 0.0037438317386317976 1105 46-47 0.010888791054142644 1105 48-49 0.03813968319121841 1105 50-51 0.04037914002333309 1105 52-53 -0.0855785042470103 1105 54-55 -0.05327459574899507 1105 56-57 0.04917459042008687 1105 58-59 -0.13238899024459272 1105 60-61 -0.10210008227654299 1105 62-63 -0.02526541682707517 1105 64-65 -0.17561865971866553 1105 66-67 0.02098071745022878 1105 68-69 -0.006581863935942067 1105 70-71 -0.17202466397338156 1105 72-73 0.02221936830503779 1105 74-75 0.06261979775258553 1105 76-77 0.05576189281859811 1105 78-79 0.14146677633551263 1105 80-81 0.1738433947037663 1105 82-83 0.06258092385273883 1105 84-85 0.007774758944570692 1105 86-87 0.08320616541319836 1105 88-89 0.24507890756337858 1105 90-91 0.176187077254486 1105 92-93 0.3562391671611138 1105 94-95 0.3152707222992781 1105 96-97 0.22036744845381762 1105 98-99 0.23807510282058786 1105 100 0.3664651020497445 1106 1 0.1206900901122836 1106 2 0.15029869106532345 1106 3 0.06528942436953145 1106 4 0.018616687762637696 1106 5 -0.0367261113287114 1106 6 0.13479287623204783 1106 7 0.11257992119217874 1106 8 0.1891520859210587 1106 9 0.2879510946144137 1106 10-11 0.20762833560969085 1106 12-13 0.22414798568084393 1106 14-15 0.30324010885495056 1106 16-17 0.25649241147436186 1106 18-19 0.24489072306528215 1106 20-21 0.22751127026616302 1106 22-23 0.24723129682936928 1106 24-25 0.25596249624289413 1106 26-27 0.13120036469506857 1106 28-29 0.29982235075238606 1106 30-31 0.27179512394534555 1106 32-33 0.31032956561497116 1106 34-35 0.12658162613604418 1106 36-37 -0.015418604788848711 1106 38-39 -0.17657807273571535 1106 40-41 -0.26438051155686537 1106 42-43 -0.4490302419726291 1106 44-45 -0.3726468977587061 1106 46-47 -0.43026396061267747 1106 48-49 -0.23129457369950757 1106 50-51 -0.428146455563315 1106 52-53 -0.4164745295600909 1106 54-55 -0.4805637133869922 1106 56-57 -0.3055038081991981 1106 58-59 -0.32815182399399134 1106 60-61 -0.42451047460865254 1106 62-63 -0.26781994164190337 1106 64-65 -0.17996163955244526 1106 66-67 0.09056598984315656 1106 68-69 -0.07535041983736335 1106 70-71 -0.0785029208230128 1106 72-73 -0.047468635722047736 1106 74-75 0.04480356409975883 1106 76-77 0.05850996403216868 1106 78-79 0.008419036945124247 1106 80-81 -0.01610482855262063 1106 82-83 -0.06858682663266791 1106 84-85 -0.03189506158326694 1106 86-87 0.05746418618553406 1106 88-89 -0.00646206694890239 1106 90-91 -0.01071258475843706 1106 92-93 -0.09350194912082088 1106 94-95 -0.15083554330308857 1106 96-97 -0.23170824421422864 1106 98-99 -0.2531850034039209 1106 100 -0.38606466444124976 1107 1 0.02468237145547647 1107 2 0.14499903914350654 1107 3 0.06828086552400237 1107 4 0.061470928869098884 1107 5 -0.09817991479841481 1107 6 0.04696545653469997 1107 7 0.07961405501301044 1107 8 0.10305120787768374 1107 9 0.22171006201324417 1107 10-11 0.20632650473691427 1107 12-13 0.09610691799486659 1107 14-15 0.16223151307804784 1107 16-17 0.22358886698591363 1107 18-19 0.1850134275496771 1107 20-21 0.08863776944905766 1107 22-23 0.025077551526578645 1107 24-25 0.1975162914030335 1107 26-27 0.12179440581814305 1107 28-29 0.06490419990620211 1107 30-31 0.007008725769750868 1107 32-33 0.2456719656002022 1107 34-35 0.3677873431124752 1107 36-37 0.11336570676421331 1107 38-39 0.07409361659757963 1107 40-41 0.060922556516885606 1107 42-43 0.26035678957600084 1107 44-45 0.06305327489545931 1107 46-47 0.22297353580960078 1107 48-49 0.5369956884209088 1107 50-51 0.43645390082442503 1107 52-53 0.42088432335148696 1107 54-55 0.19825568296972307 1107 56-57 0.27935525245134585 1107 58-59 0.46794083681906073 1107 60-61 0.5272456121431404 1107 62-63 0.3941795228532001 1107 64-65 0.4135832182156065 1107 66-67 0.38330020398971953 1107 68-69 0.4092676636090715 1107 70-71 0.7111244416307088 1107 72-73 0.5903984852005024 1107 74-75 0.6397725879112599 1107 76-77 0.7245234070516773 1107 78-79 0.6247228528791631 1107 80-81 0.6722982589101001 1107 82-83 0.4702442031063221 1107 84-85 0.6209708129054654 1107 86-87 0.5395709363171015 1107 88-89 0.49686175655606846 1107 90-91 0.43419504064665304 1107 92-93 0.48669914220122834 1107 94-95 0.5127658194645512 1107 96-97 0.38749468444359536 1107 98-99 0.5925203396423662 1107 100 0.5771978529857407 1108 1 0.1811639514292409 1108 2 0.2651369677326656 1108 3 0.2957566659707638 1108 4 0.037023175084044624 1108 5 0.1384527129362212 1108 6 0.03469725811608981 1108 7 0.09887619024684824 1108 8 0.12459146955197298 1108 9 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2215 9 -0.21936890121761365 2215 10-11 -0.19846769142255738 2215 12-13 -0.1786713477418047 2215 14-15 -0.1391656280568867 2215 16-17 -0.10451325994080918 2215 18-19 -0.020467498998158362 2215 20-21 -0.12895243118815358 2215 22-23 -0.12325836511855925 2215 24-25 -0.2787172399842035 2215 26-27 -0.42742844788029544 2215 28-29 -0.40060858748723405 2215 30-31 -0.44160489689450344 2215 32-33 -0.5322923846968877 2215 34-35 -0.5136817002010545 2215 36-37 -0.48073142575968575 2215 38-39 -0.37673734869993325 2215 40-41 -0.2775989280262934 2215 42-43 -0.40369427388736057 2215 44-45 -0.5143169136863008 2215 46-47 -0.7470277558463039 2215 48-49 -0.829662367206403 2215 50-51 -0.9150917807393384 2215 52-53 -0.6741815510995508 2215 54-55 -0.7305525491838907 2215 56-57 -0.8042794672593203 2215 58-59 -0.8905690228209977 2215 60-61 -0.9536378804944192 2215 62-63 -0.8662398347632703 2215 64-65 -0.9673691627367731 2215 66-67 -0.8775174141968023 2215 68-69 -0.7652888678642711 2215 70-71 -0.9188269059969549 2215 72-73 -0.7056598115359165 2215 74-75 -0.8759640865053413 2215 76-77 -0.9479987663746563 2215 78-79 -0.9879525970476415 2215 80-81 -1.0565508756804434 2215 82-83 -0.9629771867154311 2215 84-85 -1.0157832558106286 2215 86-87 -1.1353192729311523 2215 88-89 -0.9941181797222391 2215 90-91 -0.9056611549961602 2215 92-93 -0.9743036133313652 2215 94-95 -0.8473478462549053 2215 96-97 -0.7514874907049496 2215 98-99 -0.8414975713194863 2215 100 -0.9028891185117196 2216 1 -0.19348590851473801 2216 2 -0.17230467559133444 2216 3 -0.23038712487890933 2216 4 -0.16559120400092553 2216 5 -0.1815752982682568 2216 6 -0.22744093049268344 2216 7 -0.1856709619473591 2216 8 -0.106967820830846 2216 9 -0.3106387424874555 2216 10-11 -0.21674166767973446 2216 12-13 -0.17845459437379674 2216 14-15 -0.26893420215318997 2216 16-17 -0.24672014269390985 2216 18-19 -0.3772435427489853 2216 20-21 -0.30555640611146373 2216 22-23 -0.434682934946494 2216 24-25 -0.44146567269061876 2216 26-27 -0.5632494429449864 2216 28-29 -0.36789550225314116 2216 30-31 -0.4460400042707917 2216 32-33 -0.5700908374112998 2216 34-35 -0.5679700822205334 2216 36-37 -0.6206874081526408 2216 38-39 -0.5279478328936094 2216 40-41 -0.48419823441551557 2216 42-43 -0.3869709178782941 2216 44-45 -0.23737280270857752 2216 46-47 0.026998521331229597 2216 48-49 -0.1510575919629744 2216 50-51 -0.08167508072600072 2216 52-53 0.010308911752254346 2216 54-55 -0.09188041366301292 2216 56-57 -0.1788126138512922 2216 58-59 -0.20392769962505497 2216 60-61 -0.3529976243919819 2216 62-63 -0.2026910819287977 2216 64-65 -0.15341024583668172 2216 66-67 -0.11904735950825795 2216 68-69 -0.5744958840040617 2216 70-71 -0.6124877036493501 2216 72-73 -0.5939317869927656 2216 74-75 -0.6789686216527357 2216 76-77 -0.7873178273323731 2216 78-79 -0.9342144351162034 2216 80-81 -0.9386370434808953 2216 82-83 -0.804513801361292 2216 84-85 -0.9150596330281822 2216 86-87 -1.0206900879238177 2216 88-89 -0.7724461775880549 2216 90-91 -0.6840725195419779 2216 92-93 -0.8752806708210272 2216 94-95 -0.7281334938472739 2216 96-97 -0.6858278935327426 2216 98-99 -0.7325706672245147 2216 100 -0.6832679367056613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 3.0 5 19.0 6 137.0 7 304.0 8 320.0 9 388.0 10 578.0 11 941.0 12 1567.0 13 2273.0 14 2520.0 15 3454.0 16 4757.0 17 6923.0 18 7875.0 19 3550.0 20 1596.0 21 1207.0 22 1195.0 23 1183.0 24 1416.0 25 1918.0 26 2503.0 27 3176.0 28 2218.0 29 2525.0 30 3154.0 31 3667.0 32 4759.0 33 5921.0 34 8352.0 35 13940.0 36 21720.0 37 48800.0 38 97824.0 39 48108.0 40 121.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.578530259366 20.588462330177027 14.627933305887197 20.205074104569782 2 16.040603259453672 22.780795790435203 39.84580796433706 21.332792985774063 3 18.764091562591464 28.345228407946916 33.05973053555174 19.830949493909873 4 11.565271375679927 19.798699815683914 39.08498161664185 29.55104719199431 5 10.42877497917397 38.7328768224786 35.96743741737936 14.870910780968064 6 25.559179419881506 40.40855317752861 17.315745797711177 16.716521604878707 7 21.058437945264078 37.43418534564554 23.36585067752508 18.141526031565302 8 23.692066896611692 33.284703502958045 21.91337449156063 21.10985510886963 9 24.505241083403824 15.705560147824604 23.371790459587853 36.41740830918372 10-11 22.060512104672366 27.361995156201253 28.904227227552404 21.673265511573977 12-13 25.372090218309012 25.811442126000077 29.850681461826078 18.965786193864833 14-15 22.015679652635388 26.31367346118281 30.948900414103647 20.721746472078156 16-17 17.163161398847908 29.878615561266336 31.970197450733806 20.98802558915195 18-19 17.101407789317268 26.006953713739854 37.98522416238626 18.90641433455661 20-21 19.17594310948722 24.484341278750005 37.899669682515686 18.44004592924709 22-23 18.228893613325916 25.170224467937974 38.22307204909412 18.37780986964199 24-25 18.82880043227666 25.33675123507616 37.83787695553726 17.99657137710992 26-27 18.22842870629331 24.606175426633236 38.81103979336976 18.354356073703695 28-29 17.88045707117412 24.677018583594005 39.49114243669435 17.951381908537517 30-31 19.066498323267577 25.325656016534108 38.006899934860755 17.600945725337557 32-33 18.016605225402586 24.738794207150345 38.999601114306486 18.244999453140583 34-35 17.684641269310205 24.73652548207151 38.994228653416805 18.584604595201483 36-37 18.621651579233557 24.68955065886528 38.21643857213412 18.472359189767037 38-39 18.665044990258682 24.17858389681762 38.38144467557623 18.774926437347464 40-41 18.084093051993396 24.255271905802395 38.37364964231813 19.286985399886085 42-43 18.71706032014754 24.28914662190759 37.54065410258432 19.45313895536055 44-45 18.71619272080983 24.716984777065143 37.00464286634212 19.56217963578291 46-47 19.368528837376292 25.30215529663302 35.432226570990196 19.89708929500049 48-49 19.507950485947998 25.183005262759657 34.452159437952076 20.85688481334027 50-51 18.93462259229922 25.517442347198344 34.22815118203538 21.319783878467057 52-53 19.00504865122449 25.68791092736768 33.83095389885955 21.476086522548282 54-55 18.917778886867485 25.898926150725295 33.045739217233105 22.13755574517412 56-57 19.52321931589537 25.27758551307847 32.25303822937626 22.9461569416499 58-59 18.843507303529034 25.331938352581385 32.98555390125146 22.839000442638124 60-61 18.99415712975035 26.204065865082814 32.16652985014567 22.635247155021165 62-63 17.95324131825681 26.595951873164058 32.40545652086435 23.04535028771478 64-65 17.898093678939127 26.817630655520997 32.24873196038763 23.035543705152246 66-67 18.07418795611634 26.564183549738996 31.830769379215408 23.530859114929257 68-69 18.610702368029592 26.74241145016685 31.72484219836771 22.922043983435856 70-71 18.970258561076573 26.35045816675404 31.587614541688513 23.091668730480876 72-73 18.80365246869703 25.932013135507358 32.17604281583594 23.088291579959666 74-75 18.95691077568323 26.35656920103051 31.459765271957107 23.22675475132915 76-77 19.231134161540623 25.8291319905118 31.55373296345435 23.386000884493225 78-79 19.449331485013495 25.5524857436003 31.916163042394498 23.082019728991714 80-81 19.474481525319064 25.585053005351998 31.50489527583368 23.435570193495263 82-83 18.920435813934787 25.421139388091508 31.849957785550597 23.808467012423108 84-85 19.154715306211063 25.55280737698327 31.108059167677133 24.184418149128533 86-87 19.31511167146974 25.55208547756278 30.766261836146562 24.366541014820914 88-89 19.18366231067855 25.394885385943976 30.637831161771945 24.78362114160553 90-91 19.36287438246192 24.824226790860436 30.801159170440513 25.011739656237136 92-93 19.479364507564124 25.226553793157798 30.588581528005825 24.705500171272256 94-95 19.14692080319322 24.965343557118 30.725389100990963 25.162346538697815 96-97 18.93381406957596 24.85027917867435 30.87835143062989 25.337555321119805 98-99 19.59454895742539 24.89168995828415 30.05229758807126 25.4614634962192 100 19.423439997684238 24.813372229530447 29.894858047106425 25.86832972567889 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 577.0 1 372.5 2 464.5 3 859.5 4 561.5 5 231.0 6 530.5 7 1022.0 8 1535.0 9 1716.5 10 1093.5 11 444.0 12 337.5 13 327.5 14 331.5 15 326.0 16 318.0 17 356.0 18 410.5 19 450.5 20 517.5 21 621.5 22 787.0 23 1041.0 24 1339.5 25 1687.0 26 2169.0 27 2845.0 28 3883.0 29 4792.0 30 5476.0 31 6498.0 32 7692.5 33 9000.5 34 10197.5 35 10985.5 36 11596.0 37 12148.0 38 12132.5 39 11806.0 40 11661.0 41 11147.5 42 10303.0 43 9637.0 44 9419.0 45 9227.0 46 9277.5 47 10309.0 48 12405.0 49 12916.5 50 12869.0 51 12138.0 52 8906.5 53 6601.5 54 5668.0 55 5223.0 56 4785.0 57 4164.0 58 3471.5 59 2745.0 60 2168.0 61 1703.0 62 1288.0 63 917.0 64 697.5 65 553.0 66 404.5 67 309.0 68 229.0 69 160.0 70 116.0 71 82.0 72 65.5 73 53.5 74 33.0 75 26.0 76 17.5 77 9.5 78 7.5 79 3.0 80 1.5 81 1.0 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.2163338297208546E-4 2 6.432667659441709E-4 3 0.0 4 0.010935535021050904 5 0.0012865335318883418 6 0.0035379672126929396 7 6.432667659441709E-4 8 0.12993988672072251 9 0.0012865335318883418 10-11 0.0 12-13 1.6081669148604273E-4 14-15 1.6081669148604273E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.2163338297208546E-4 26-27 0.006271850967955666 28-29 0.006593484350927751 30-31 0.01302615201036946 32-33 0.015116768999688014 34-35 0.021549436659129725 36-37 0.036505388967331694 38-39 0.03972172279705256 40-41 0.05210460804147784 42-43 0.06834709388156816 44-45 0.1058173829978161 46-47 0.11337576749766011 48-49 0.10758636660416257 50-51 0.11160678389131365 52-53 0.10774718329564863 54-55 0.11192841727428572 56-57 0.0926304142959606 58-59 0.08860999700880953 60-61 0.08941408046623976 62-63 0.08860999700880953 64-65 0.0 66-67 0.0 68-69 1.6081669148604273E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 1.6081669148604273E-4 78-79 0.0 80-81 3.2163338297208546E-4 82-83 1.6081669148604273E-4 84-85 0.0 86-87 3.2163338297208546E-4 88-89 0.0 90-91 3.2163338297208546E-4 92-93 0.0014473502233743843 94-95 0.0012865335318883418 96-97 3.2163338297208546E-4 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 310913.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.94457564884364 #Duplication Level Percentage of deduplicated Percentage of total 1 84.29987696681023 54.74819736859218 2 11.144304552910153 14.475242601804519 3 2.384470516396276 4.645752775036055 4 0.7516928353436881 1.9527348883868767 5 0.3485948009723789 1.1319670711272125 6 0.184418745575146 0.7186198303841951 7 0.11414383588962296 0.5189116089347968 8 0.0948587052008956 0.49284466846967373 9 0.07360025722741433 0.43019437259521437 >10 0.49021169750798155 6.359367011147429 >50 0.06780135132508355 2.9634089423270793 >100 0.03959202997085133 4.991833436364591 >500 0.0029694022478138496 1.506453114174286 >1k 0.0034643026224494915 5.064472310655905 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4158 1.3373516063979314 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3779 1.2154525542515109 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2265 0.7284996124317735 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 2069 0.6654594693692448 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1217 0.39142782707702795 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1194 0.38403025926867 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1075 0.34575588669499185 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 968 0.31134111471697873 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 941 0.3026570133767324 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 858 0.2759614425900493 TruSeq Adapter, Index 1 (95% over 21bp) GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 708 0.22771643514423648 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 682 0.2193539671869623 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 530 0.17046569297520528 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 500 0.16081669148604272 RNA PCR Primer, Index 1 (95% over 23bp) GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 471 0.15148932337985224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 400 0.1286533531888342 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 396 0.12736681965694582 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 387 0.12447211921019707 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 356 0.11450148433806243 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 351 0.11289331742320198 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 349 0.11225005065725782 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 347 0.11160678389131365 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 343 0.1103202503594253 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 325 0.10453084946592778 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 311 0.10002798210431857 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.6081669148604273E-4 0.0 14-15 0.0 0.0 0.0 6.432667659441709E-4 0.0 16-17 0.0 0.0 0.0 0.001125716840402299 0.0 18-19 0.0 0.0 0.0 0.0014473502233743846 0.0 20-21 0.0 0.0 0.0 0.0019298002978325127 0.0 22-23 0.0 0.0 0.0 0.005628584202011495 0.0 24-25 0.0 0.0 0.0 0.018815552903866998 0.0 26-27 0.0 0.0 0.0 0.049531540977701155 0.0 28-29 0.0 0.0 0.0 0.12736681965694585 0.0 30-31 0.0 0.0 0.0 0.28625371084515605 0.0 32-33 0.0 0.0 0.0 0.5374493829463548 0.0 34-35 0.0 0.0 0.0 0.9462454127038754 0.0 36-37 0.0 0.0 0.0 1.5042793321604435 0.0 38-39 0.0 0.0 0.0 2.1449730310408377 0.0 40-41 0.0 0.0 0.0 2.812040667324943 0.0 42-43 0.0 0.0 0.0 3.3369463483353865 0.0 44-45 0.0 0.0 0.0 3.850755677633293 0.0 46-47 0.0 0.0 0.0 4.430339033748991 0.0 48-49 0.0 0.0 0.0 5.0367787773428585 0.0 50-51 0.0 0.0 0.0 5.571333459842464 0.0 52-53 0.0 0.0 0.0 6.085625239214829 0.0 54-55 0.0 0.0 0.0 6.658293477596627 0.0 56-57 0.0 0.0 0.0 7.291750425360149 0.0 58-59 0.0 0.0 0.0 7.887737084007424 0.0 60-61 0.0 0.0 0.0 8.459762055623276 0.0 62-63 0.0 0.0 0.0 9.017956791771333 0.0 64-65 0.0 0.0 0.0 9.61088793328037 0.0 66-67 0.0 0.0 0.0 10.320250359425305 0.0 68-69 0.0 0.0 0.0 11.095065179005058 0.0 70-71 0.0 0.0 0.0 11.895610669222581 0.0 72-73 0.0 0.0 0.0 12.693261458993351 0.0 74-75 0.0 0.0 0.0 13.500722066944771 0.0 76-77 0.0 0.0 0.0 14.33873784627854 0.0 78-79 0.0 0.0 0.0 15.273726090578393 0.0 80-81 0.0 0.0 0.0 16.210000868410134 0.0 82-83 0.0 0.0 0.0 17.138234811667573 0.0 84-85 0.0 0.0 0.0 18.08641002466928 0.0 86-87 0.0 0.0 0.0 19.08202616165937 0.0 88 0.0 0.0 0.0 19.85378546410089 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGAC 55 0.002706711 34.200497 5 GGCTTAG 55 0.0027174142 34.172985 1 CGGTACT 70 2.1846642E-4 33.56275 1 ATGGGCA 175 5.487891E-9 26.871817 5 GGTATCA 4410 0.0 25.251974 1 CTCTAGC 95 0.0012958683 24.758331 2 CGGAACA 115 1.3794762E-4 24.507515 94 CACGATG 100 0.0017502307 23.51284 7 GTATCAA 5830 0.0 23.373032 1 ACTGTAC 105 0.0023243013 22.393183 8 GAGTACT 4100 0.0 22.226128 12-13 TAGATGT 130 3.1374354E-4 21.707653 4 TTATATG 110 0.0030392278 21.382196 2 TATCAAC 6455 0.0 20.696507 2 CAACGCA 6525 0.0 20.684092 5 ATCAACG 6490 0.0 20.581577 3 GTCCAGG 160 5.4073396E-5 20.557186 1 TCAACGC 6555 0.0 20.44924 4 TAGCCTC 115 0.003939753 20.445948 5 GTACCCA 115 0.0039581633 20.4295 1 >>END_MODULE