FastQCFastQC Report
Wed 25 May 2016
SRR1032986_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032986_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2715919
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22100.039.557811
GTATCAA37200.035.251111
TCAACGC36700.033.6822624
ATCAACG37750.032.869913
CAACGCA38150.032.4026685
AACGCAG40150.030.6709546
TATCAAC44000.029.055462
GTACATG84050.026.6742521
TACATGG84250.025.4393772
ACATGGG83550.024.1336153
ACGCAGA50850.023.847367
CGCAGAG51950.022.8896188
GAGTACA60900.021.7642331
CATGGGG54950.019.673054
TACACCG3354.0017767E-1119.6427755
GTGTAGG11100.019.0546551
GCAGAGT62000.018.3454029
AGTACAT64950.018.0190452
TATACTG17600.017.3588245
CGTATAC1902.0236606E-417.3163363
Produced by FastQC (version 0.11.5)