##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032986_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2715919 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.64837463856617 33.0 31.0 34.0 30.0 34.0 2 31.76433612342636 34.0 31.0 34.0 30.0 34.0 3 31.846870617275403 34.0 31.0 34.0 30.0 34.0 4 35.19782585563119 37.0 35.0 37.0 33.0 37.0 5 35.268417430711295 37.0 35.0 37.0 33.0 37.0 6 35.39086695884524 37.0 36.0 37.0 35.0 37.0 7 35.35556141401861 37.0 37.0 37.0 35.0 37.0 8 35.366800335356096 37.0 37.0 37.0 35.0 37.0 9 37.04280945050276 39.0 38.0 39.0 35.0 39.0 10-11 37.03292587150059 39.0 38.0 39.0 35.0 39.0 12-13 36.9800198385887 39.0 38.0 39.0 35.0 39.0 14-15 38.48243449086662 41.0 39.0 41.0 35.0 41.0 16-17 38.507814850148335 41.0 39.0 41.0 35.0 41.0 18-19 38.524462622044325 41.0 39.5 41.0 35.0 41.0 20-21 38.567958580502584 41.0 39.5 41.0 35.0 41.0 22-23 38.56385959964196 41.0 39.0 41.0 35.0 41.0 24-25 38.601219881741685 41.0 39.0 41.0 35.0 41.0 26-27 38.57979821931361 41.0 39.0 41.0 34.5 41.0 28-29 38.56418656079212 41.0 39.0 41.0 34.0 41.0 30-31 38.52452392725998 41.0 39.0 41.0 34.0 41.0 32-33 38.47533560463327 41.0 39.0 41.0 34.0 41.0 34-35 38.41285399159548 41.0 39.0 41.0 34.0 41.0 36-37 38.33078913620031 41.0 39.0 41.0 34.0 41.0 38-39 38.24602096012437 41.0 39.0 41.0 34.0 41.0 40-41 38.169601155262725 41.0 39.0 41.0 33.0 41.0 42-43 38.06945457504439 41.0 38.0 41.0 33.0 41.0 44-45 37.953482596498645 40.0 38.0 41.0 33.0 41.0 46-47 37.844142811328325 40.0 38.0 41.0 33.0 41.0 48-49 37.725587545136655 40.0 38.0 41.0 33.0 41.0 50-51 37.601637972266474 40.0 38.0 41.0 32.5 41.0 52-53 37.4573161055245 40.0 37.5 41.0 32.0 41.0 54-55 37.3081106248014 40.0 37.0 41.0 32.0 41.0 56-57 37.138189872378376 40.0 37.0 41.0 31.5 41.0 58-59 36.9425914395827 40.0 36.0 41.0 31.0 41.0 60-61 36.71140597344766 39.5 36.0 41.0 31.0 41.0 62-63 36.46795688678492 39.0 35.0 41.0 31.0 41.0 64-65 36.19982241738432 39.0 35.0 41.0 30.5 41.0 66-67 35.91050322192967 38.0 35.0 40.0 30.0 41.0 68-69 35.60650888336508 37.5 35.0 40.0 30.0 41.0 70-71 35.270264503470095 37.0 35.0 40.0 30.0 41.0 72-73 34.9740111910554 37.0 35.0 39.0 29.5 41.0 74-75 34.693728531668285 36.0 34.5 39.0 29.5 41.0 76-77 34.35616912728251 36.0 34.0 39.0 29.0 40.5 78-79 33.98034753613786 35.0 34.0 37.5 29.0 39.5 80-81 33.64378797747651 35.0 34.0 37.0 29.0 39.0 82-83 33.3142788868151 35.0 34.0 37.0 29.0 39.0 84-85 33.13094573144487 35.0 34.0 36.0 29.0 38.0 86-87 32.88495846157415 35.0 34.0 36.0 29.0 37.0 88-89 32.64035249210304 35.0 34.0 36.0 28.0 37.0 90-91 32.43736447957395 35.0 34.0 35.0 28.0 36.5 92-93 32.23954617203238 35.0 34.0 35.0 27.0 36.0 94-95 32.0586471835132 35.0 34.0 35.0 27.0 36.0 96-97 31.886949316235132 35.0 34.0 35.0 27.0 36.0 98-99 31.714393544137362 35.0 33.0 35.0 26.0 35.5 100 31.592346826249237 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24150189118614307 1101 2 0.31412759798435275 1101 3 0.33073178102432266 1101 4 0.24440811247097116 1101 5 0.17318171825009898 1101 6 0.2069855328697443 1101 7 0.1795052031365998 1101 8 0.14647032610552202 1101 9 0.17696109043441055 1101 10-11 0.18407896963517345 1101 12-13 -0.029579992124915577 1101 14-15 0.0018890193592895343 1101 16-17 0.01037706228390789 1101 18-19 0.08278925696219375 1101 20-21 0.0911128745181955 1101 22-23 0.09719543647634765 1101 24-25 0.0748339675205969 1101 26-27 0.14072264320309813 1101 28-29 0.1308652597405171 1101 30-31 0.1693470937778585 1101 32-33 0.10845177728099031 1101 34-35 0.1225950552864532 1101 36-37 0.16427953165616316 1101 38-39 0.18429419778893674 1101 40-41 0.15551561363396615 1101 42-43 0.09397841290191167 1101 44-45 0.059361150140389896 1101 46-47 0.0497130522628666 1101 48-49 0.1009823151567133 1101 50-51 0.07837690126295627 1101 52-53 0.024101753586705854 1101 54-55 0.08100565979091812 1101 56-57 0.053007157530430504 1101 58-59 0.037535520384523124 1101 60-61 0.06499713665019868 1101 62-63 0.06447632418512228 1101 64-65 0.062076591499760525 1101 66-67 -0.0282968855732193 1101 68-69 -0.0056205985597159724 1101 70-71 0.028343886106071636 1101 72-73 -0.03384235908772126 1101 74-75 0.053037419348420656 1101 76-77 0.10147832001713653 1101 78-79 0.091557247268085 1101 80-81 0.01599513742259262 1101 82-83 -0.03245844919977969 1101 84-85 -0.03514149426905533 1101 86-87 -0.054490852877030704 1101 88-89 -0.04428835440383949 1101 90-91 -0.028715028587747327 1101 92-93 -0.011035254610582967 1101 94-95 -0.02542984983091401 1101 96-97 -0.07907892995935484 1101 98-99 -0.1016476884820392 1101 100 -0.08650445833178821 1102 1 0.38924138472327385 1102 2 0.3848077777175547 1102 3 0.39569226957823744 1102 4 0.4173815026242593 1102 5 0.3009311227996889 1102 6 0.37590437729217285 1102 7 0.37081292338620386 1102 8 0.4203034653010107 1102 9 0.39784063766264666 1102 10-11 0.39866155241642076 1102 12-13 0.4876085075247971 1102 14-15 0.5071305175968561 1102 16-17 0.4677260363034108 1102 18-19 0.5444354117664858 1102 20-21 0.5176258161966913 1102 22-23 0.5229424797853284 1102 24-25 0.5495503072519838 1102 26-27 0.544930815586774 1102 28-29 0.5383493405864925 1102 30-31 0.5530687325932675 1102 32-33 0.6073868500178676 1102 34-35 0.6254186955840453 1102 36-37 0.5995685318328654 1102 38-39 0.6112033862748731 1102 40-41 0.6310849381266621 1102 42-43 0.6585345345314195 1102 44-45 0.6217213388608442 1102 46-47 0.6504484666704258 1102 48-49 0.6544351825711416 1102 50-51 0.6120616047207648 1102 52-53 0.7172995986715591 1102 54-55 0.6415689932762234 1102 56-57 0.6626650905270992 1102 58-59 0.5856899742463213 1102 60-61 0.6719043614087852 1102 62-63 0.598552283185839 1102 64-65 0.6935667625824209 1102 66-67 0.7110147311405726 1102 68-69 0.8153235967188195 1102 70-71 0.7784080275802552 1102 72-73 0.6723461831739783 1102 74-75 0.6708378182323997 1102 76-77 0.5597222948158702 1102 78-79 0.6043693763687017 1102 80-81 0.6416377813565077 1102 82-83 0.608368366436018 1102 84-85 0.5798406280643107 1102 86-87 0.590545461179893 1102 88-89 0.6034277472367862 1102 90-91 0.5172211434192775 1102 92-93 0.45788101223344313 1102 94-95 0.50042311258143 1102 96-97 0.4431099228037034 1102 98-99 0.4634787037455297 1102 100 0.5758126565206361 1103 1 0.1014303040597504 1103 2 0.18922130953624006 1103 3 0.23827958344080358 1103 4 0.18709210510182572 1103 5 0.16454765314116315 1103 6 0.10349324989514486 1103 7 0.2292721185479607 1103 8 0.21609383280204497 1103 9 0.27391862290887303 1103 10-11 0.33412962809254765 1103 12-13 0.3055834396492685 1103 14-15 0.3214145648792126 1103 16-17 0.34210218131735814 1103 18-19 0.34611124428599993 1103 20-21 0.30874678019294066 1103 22-23 0.337382759933746 1103 24-25 0.2687387747546808 1103 26-27 0.358964904636494 1103 28-29 0.3861327428431309 1103 30-31 0.28833856404836666 1103 32-33 0.28216449268567345 1103 34-35 0.26619764093397436 1103 36-37 0.29099139772205973 1103 38-39 0.25323212278804164 1103 40-41 0.31992971054768304 1103 42-43 0.2779378527251666 1103 44-45 0.27324798585809873 1103 46-47 0.31048094186406416 1103 48-49 0.3040759833331137 1103 50-51 0.2769677989771324 1103 52-53 0.3153517616463404 1103 54-55 0.32836063475330945 1103 56-57 0.3184778430330013 1103 58-59 0.33919513072032004 1103 60-61 0.2888293397787507 1103 62-63 0.2590746994803297 1103 64-65 0.2952942373483438 1103 66-67 0.24329465163620512 1103 68-69 0.26275880852092115 1103 70-71 0.2998137961253917 1103 72-73 0.30605632692831364 1103 74-75 0.27560088969161 1103 76-77 0.2997411173956195 1103 78-79 0.3101671012392515 1103 80-81 0.26997960020847955 1103 82-83 0.3216128085074317 1103 84-85 0.2539987216587747 1103 86-87 0.2386867996068318 1103 88-89 0.24597749918750367 1103 90-91 0.2660151290352246 1103 92-93 0.24259004161756792 1103 94-95 0.1965122960713046 1103 96-97 0.17228703130868084 1103 98-99 0.2798071983812278 1103 100 0.34504903447872337 1104 1 0.12027678805479525 1104 2 0.11628994930604009 1104 3 0.15498165961113486 1104 4 0.05900619791862738 1104 5 0.1581539254136004 1104 6 0.14570945411231406 1104 7 0.16793802649871736 1104 8 0.09786184519551 1104 9 0.1461370441889187 1104 10-11 0.12443016435670984 1104 12-13 0.14938104058290946 1104 14-15 0.2432114336023119 1104 16-17 0.18760223391393538 1104 18-19 0.21562839670480116 1104 20-21 0.19148772564463457 1104 22-23 0.3083824454587045 1104 24-25 0.26158163745498797 1104 26-27 0.295297788759207 1104 28-29 0.2699148864585581 1104 30-31 0.2550301968309654 1104 32-33 0.26766043557103814 1104 34-35 0.3545402801333566 1104 36-37 0.27496307536497966 1104 38-39 0.33059902377864603 1104 40-41 0.3123210503080216 1104 42-43 0.2824725124860876 1104 44-45 0.2770482607959863 1104 46-47 0.279636813808942 1104 48-49 0.2679081088779611 1104 50-51 0.2667914320391418 1104 52-53 0.30918163837708335 1104 54-55 0.31764032123908237 1104 56-57 0.36180119820288326 1104 58-59 0.3741035174301999 1104 60-61 0.43169126010860737 1104 62-63 0.37387237937786466 1104 64-65 0.35289438688273833 1104 66-67 0.3542452235583511 1104 68-69 0.3969743906904384 1104 70-71 0.3055431987691364 1104 72-73 0.3925914593681412 1104 74-75 0.4085147411991912 1104 76-77 0.3713484175790285 1104 78-79 0.3191026860372119 1104 80-81 0.278625834832269 1104 82-83 0.2511593542654822 1104 84-85 0.23706020939907546 1104 86-87 0.18794104646946863 1104 88-89 0.16672830352853651 1104 90-91 0.1819631484360471 1104 92-93 0.18632444383473512 1104 94-95 0.11498874730185094 1104 96-97 0.19038415219162275 1104 98-99 0.21831693868668367 1104 100 0.17236744584687358 1105 1 0.040538869860242244 1105 2 0.05236273363997057 1105 3 0.020323923015634904 1105 4 0.1504555414717288 1105 5 0.04285395282560245 1105 6 0.10496933401235964 1105 7 0.15526409992511248 1105 8 0.1413207032381152 1105 9 0.18616035731211156 1105 10-11 0.1859413866795805 1105 12-13 0.22440962042847445 1105 14-15 0.27244865039547506 1105 16-17 0.27905932995513894 1105 18-19 0.23999824366074307 1105 20-21 0.2301299031952979 1105 22-23 0.24149129456606033 1105 24-25 0.2852561458627605 1105 26-27 0.2596712296891184 1105 28-29 0.2605911280443749 1105 30-31 0.29043282826396677 1105 32-33 0.24638820456598154 1105 34-35 0.28353066963227036 1105 36-37 0.3013929969475697 1105 38-39 0.23592494334797465 1105 40-41 0.28063643265576843 1105 42-43 0.27501471832339064 1105 44-45 0.16889429320178095 1105 46-47 0.16656240165129077 1105 48-49 0.16929646022788347 1105 50-51 0.20252698314065753 1105 52-53 0.23975711352012752 1105 54-55 0.2139474135928765 1105 56-57 0.15832512168542223 1105 58-59 0.14231376411238728 1105 60-61 0.17426810366519163 1105 62-63 0.1874931232867425 1105 64-65 0.29361359514300034 1105 66-67 0.2782636840215602 1105 68-69 0.24476681006903078 1105 70-71 0.22741551210960154 1105 72-73 0.2724786825322596 1105 74-75 0.2603779125671508 1105 76-77 0.22112308853063212 1105 78-79 0.25010802670638554 1105 80-81 0.12126861429405977 1105 82-83 0.2680474538583155 1105 84-85 0.2437257589123334 1105 86-87 0.19940419342702143 1105 88-89 0.21102472845870324 1105 90-91 0.17690663776733118 1105 92-93 0.03988242837071709 1105 94-95 0.03780845444465797 1105 96-97 0.10281184781621633 1105 98-99 0.11111483748411644 1105 100 0.052097730822371346 1106 1 -0.024579227393651593 1106 2 0.019939557619373716 1106 3 0.019716499957830536 1106 4 0.027706526548122667 1106 5 0.020680110918895878 1106 6 0.07749961295471763 1106 7 0.09252216550902403 1106 8 0.10282966468276555 1106 9 0.1882279537761491 1106 10-11 0.15987982283316882 1106 12-13 0.20249415714859964 1106 14-15 0.20274058104433124 1106 16-17 0.18256941431910434 1106 18-19 0.18431613638065159 1106 20-21 0.1791306720892365 1106 22-23 0.1953865667352801 1106 24-25 0.171986247067359 1106 26-27 0.22244522499631358 1106 28-29 0.16641448293763972 1106 30-31 0.1430108612258394 1106 32-33 0.21548336551511937 1106 34-35 0.1660221444931551 1106 36-37 0.15887262630223375 1106 38-39 0.13845321414996192 1106 40-41 0.1645867568742858 1106 42-43 0.19724577804459642 1106 44-45 0.12608666706015015 1106 46-47 0.15302132509259536 1106 48-49 0.20036027968565406 1106 50-51 0.18069660574582969 1106 52-53 0.25121618670502244 1106 54-55 0.26207907068068437 1106 56-57 0.16574083417016539 1106 58-59 0.19666863877068863 1106 60-61 0.24013167180593342 1106 62-63 0.15965235337139916 1106 64-65 0.20755639435197537 1106 66-67 0.1909124779236251 1106 68-69 0.18902918732263174 1106 70-71 0.18871729147329575 1106 72-73 0.13036583513106592 1106 74-75 0.125780491120274 1106 76-77 0.16162939210534688 1106 78-79 0.21986666210406725 1106 80-81 0.23095538537771887 1106 82-83 0.18856759058839856 1106 84-85 0.1583710920035557 1106 86-87 0.14370815211166388 1106 88-89 0.09353956331990076 1106 90-91 0.08410567915614564 1106 92-93 0.10072826214145891 1106 94-95 0.09825928038301868 1106 96-97 0.06398421719173086 1106 98-99 0.11383771190535441 1106 100 0.11581889375317189 1107 1 0.06726457409168418 1107 2 0.14375871332975265 1107 3 0.13193093936633815 1107 4 0.1807795488424091 1107 5 0.12904945983213167 1107 6 0.2164523280508206 1107 7 0.20849708927134003 1107 8 0.1777622859689103 1107 9 0.21180298241915807 1107 10-11 0.18821390234153768 1107 12-13 0.19382615985266227 1107 14-15 0.2786634747051764 1107 16-17 0.33066116828230463 1107 18-19 0.2755738783182409 1107 20-21 0.24843763898581983 1107 22-23 0.22569685069204581 1107 24-25 0.24818635515869403 1107 26-27 0.2687049854711532 1107 28-29 0.22695532011629638 1107 30-31 0.23254154982945607 1107 32-33 0.18923664378014138 1107 34-35 0.20801396213392565 1107 36-37 0.26795417104508346 1107 38-39 0.24488215123403023 1107 40-41 0.19709894093158198 1107 42-43 0.18113746434666922 1107 44-45 0.21580702087197068 1107 46-47 0.28731707558117137 1107 48-49 0.2308951593552706 1107 50-51 0.20870528159134238 1107 52-53 0.20015489392555708 1107 54-55 0.19570941735182146 1107 56-57 0.2139440684673417 1107 58-59 0.23976604404361979 1107 60-61 0.20349973943852007 1107 62-63 0.17415511243534354 1107 64-65 0.16824212623671997 1107 66-67 0.18996218982871937 1107 68-69 0.18315955450615462 1107 70-71 0.2171755749377553 1107 72-73 0.2753615572590533 1107 74-75 0.1943744892569228 1107 76-77 0.19381462663915983 1107 78-79 0.1993046050154419 1107 80-81 0.21462512176686488 1107 82-83 0.1628003532018809 1107 84-85 0.19048301706973803 1107 86-87 0.09316933049744591 1107 88-89 0.15205966337275356 1107 90-91 0.19660723244879819 1107 92-93 0.22766030551355243 1107 94-95 0.2231160058577899 1107 96-97 0.16526977867506432 1107 98-99 0.12410635870057973 1107 100 0.17258301504139695 1108 1 -0.052676872621418624 1108 2 0.01872888751252688 1108 3 -0.07675698979403123 1108 4 -0.06793936129382416 1108 5 -0.09385828276065666 1108 6 -0.09922601518657359 1108 7 -0.07724181457779622 1108 8 -0.05176584119701744 1108 9 -0.037667727681473195 1108 10-11 -0.04966166624269164 1108 12-13 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-0.1937865757237205 2215 5 -0.1190135382308668 2215 6 -0.18591757431907752 2215 7 -0.1925468117725586 2215 8 -0.21350560529898388 2215 9 -0.28322126260508185 2215 10-11 -0.24321590408826665 2215 12-13 -0.24011752409101916 2215 14-15 -0.26002618898449015 2215 16-17 -0.30023108760995854 2215 18-19 -0.29070018795582087 2215 20-21 -0.28482939428121057 2215 22-23 -0.35420772921253274 2215 24-25 -0.2768809199085851 2215 26-27 -0.2919270751609062 2215 28-29 -0.3012287080677325 2215 30-31 -0.3033856110977027 2215 32-33 -0.36908628954279266 2215 34-35 -0.33708795796799507 2215 36-37 -0.3241834210925063 2215 38-39 -0.30357539722351845 2215 40-41 -0.2584475146148719 2215 42-43 -0.3155345323253229 2215 44-45 -0.3251283950634374 2215 46-47 -0.3048725166084125 2215 48-49 -0.29027861181850767 2215 50-51 -0.26500381711775844 2215 52-53 -0.34280852403786355 2215 54-55 -0.2970885754461676 2215 56-57 -0.30647570195242224 2215 58-59 -0.26466533538776105 2215 60-61 -0.24490483396605356 2215 62-63 -0.19291975463952582 2215 64-65 -0.23417815118355634 2215 66-67 -0.31624044137392104 2215 68-69 -0.24836450201790683 2215 70-71 -0.1975394647772788 2215 72-73 -0.22739607049857824 2215 74-75 -0.18356188867946344 2215 76-77 -0.2638085809840476 2215 78-79 -0.2006359199250909 2215 80-81 -0.28696412982238684 2215 82-83 -0.26457832417679583 2215 84-85 -0.23136740478068418 2215 86-87 -0.25488489255678104 2215 88-89 -0.24056022924714426 2215 90-91 -0.22002693418536978 2215 92-93 -0.22234164448840232 2215 94-95 -0.1801395223977309 2215 96-97 -0.18836957496428752 2215 98-99 -0.25822727363305376 2215 100 -0.2642986718402902 2216 1 -0.04389402432806122 2216 2 -0.10252578773181753 2216 3 -0.17301877004197053 2216 4 -0.0932620500399608 2216 5 -0.10302867670460358 2216 6 -0.1549520401688298 2216 7 -0.14519604467657388 2216 8 -0.2191734039604114 2216 9 -0.24418700541796312 2216 10-11 -0.2689433541787807 2216 12-13 -0.27351789029393103 2216 14-15 -0.35072335691095446 2216 16-17 -0.29991744159567446 2216 18-19 -0.3661604194670076 2216 20-21 -0.37962873908055883 2216 22-23 -0.40353296643586134 2216 24-25 -0.4609017966046238 2216 26-27 -0.43208054652456696 2216 28-29 -0.42369280505411666 2216 30-31 -0.4834269002154272 2216 32-33 -0.5670347446568513 2216 34-35 -0.4873687396182049 2216 36-37 -0.5329047365017132 2216 38-39 -0.43967038045226303 2216 40-41 -0.4716694221042985 2216 42-43 -0.4238056581123715 2216 44-45 -0.4553058365466143 2216 46-47 -0.42037249835043866 2216 48-49 -0.42590410413188806 2216 50-51 -0.4067072605727944 2216 52-53 -0.47116016751689216 2216 54-55 -0.5186322094101854 2216 56-57 -0.45562774919364557 2216 58-59 -0.48652783193178095 2216 60-61 -0.4981658396155879 2216 62-63 -0.42846725929530294 2216 64-65 -0.4750132972734775 2216 66-67 -0.45526778661145784 2216 68-69 -0.43267424604102445 2216 70-71 -0.46853809803705815 2216 72-73 -0.5397672865322178 2216 74-75 -0.5312407777596277 2216 76-77 -0.5508905384481722 2216 78-79 -0.4846068480042973 2216 80-81 -0.45973678667147766 2216 82-83 -0.5059266499200206 2216 84-85 -0.5424207858977539 2216 86-87 -0.4829681437292024 2216 88-89 -0.43162216553200494 2216 90-91 -0.4868347089549232 2216 92-93 -0.49142775433884367 2216 94-95 -0.4480864647077354 2216 96-97 -0.44363700220623414 2216 98-99 -0.5113741669073377 2216 100 -0.4852632670087047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31923.0 3 5007.0 4 3326.0 5 2786.0 6 2306.0 7 2520.0 8 2911.0 9 3189.0 10 3378.0 11 3503.0 12 3909.0 13 4311.0 14 4676.0 15 4971.0 16 4893.0 17 4789.0 18 4675.0 19 4748.0 20 4731.0 21 5096.0 22 6031.0 23 8666.0 24 13457.0 25 18455.0 26 19684.0 27 16401.0 28 17010.0 29 21358.0 30 25964.0 31 31364.0 32 40762.0 33 55254.0 34 80827.0 35 126608.0 36 239585.0 37 526979.0 38 921337.0 39 437606.0 40 923.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.366063670872926 19.899008583238697 13.095832040602911 23.639095705285467 2 15.306411971273072 20.215194024680986 40.760143413552065 23.718250590493877 3 18.200289409363908 25.735434738630623 29.83425841890513 26.23001743310034 4 12.028839765446598 16.84106843916893 39.33301962264068 31.797072172743796 5 12.743791712850506 38.50497753696672 35.631986533971656 13.119244216211118 6 30.66596359270793 38.49484688795809 17.943715023636624 12.895474495697357 7 27.29992479227597 32.80386997354648 22.567955024233395 17.328250209944148 8 24.967776026247893 37.13380926797596 20.27884699710887 17.619567708667272 9 24.859580216424998 17.37126205907746 21.151864138723656 36.61729358577389 10-11 23.620700106104596 27.44343191535657 29.01911927840767 19.916748700131162 12-13 24.15057241409551 24.730466638441158 29.128045434965806 21.990915512497526 14-15 21.497424824306783 26.828899818249987 25.93689691098671 25.73677844645652 16-17 21.31079948038482 30.23340861771935 26.527898732889906 21.927893169005923 18-19 21.195573674327843 29.23617804936285 28.451600327788633 21.11664794852068 20-21 22.351050294533472 28.517958859272802 27.939319509217736 21.19167133697599 22-23 21.977980474293634 28.224080427147623 28.037394025113954 21.760545073444785 24-25 21.811300440102702 28.389332447035866 27.973309377754322 21.826057735107106 26-27 21.55792952820134 28.357902629865073 28.047189998977494 22.03697784295609 28-29 21.66590457648358 28.335642939440177 28.157715453825627 21.84073703025062 30-31 21.846366087388116 28.774218438236144 27.72563251388319 21.653782960492546 32-33 21.50026732322676 28.543801398924 28.19325977000991 21.762671507839332 34-35 21.65698571466207 28.229611830332757 28.296781897906286 21.816620557098886 36-37 21.94775879224055 28.571910186256243 27.72814469414813 21.752186327355073 38-39 21.76455936770346 28.738969868937225 27.903131969537696 21.593338793821616 40-41 21.563138074334837 28.42673840396186 28.20987948863964 21.800244033063663 42-43 21.520312471212485 28.345542310739358 28.202848351972293 21.93129686607587 44-45 21.626075786674765 28.474870183371976 28.16204637876829 21.737007651184967 46-47 21.650598757988277 28.603800839615403 28.009750372410057 21.735850029986263 48-49 21.553736477369647 28.48059925292379 28.09943303205964 21.86623123764692 50-51 21.61465626778065 28.395975271445124 28.031186339743456 21.95818212103077 52-53 21.636158977419527 28.364863647276657 28.106922690125742 21.892054685178078 54-55 21.728994736350433 28.735285061992112 27.896845219000582 21.638874982656876 56-57 21.55201767050869 28.491263136249966 27.94512163214889 22.01159756109245 58-59 21.469934202538116 28.126015101485734 28.32576035765106 22.07829033832509 60-61 21.806747856437852 28.478621310500113 27.904750797369925 21.809880035692103 62-63 21.533166860463297 28.54232815004889 27.951762787767727 21.972742201720088 64-65 21.529723486299417 28.23778641643189 28.172452986553953 22.060037110714738 66-67 21.404056397941325 28.298333656989293 28.045310851588575 22.252299093480808 68-69 21.790676949171466 28.39847660135666 27.952165238380637 21.858681211091234 70-71 21.7045061570199 28.180297867957872 28.06724828735228 22.04794768766995 72-73 21.461355638668312 28.27282274259815 28.210549588223373 22.05527203051016 74-75 21.526185009128103 28.352134579695466 28.055860419330834 22.065819991845597 76-77 21.518010176248406 28.311743918321763 27.970294065464113 22.199951839965717 78-79 21.587328141213096 28.398817033770403 28.057107672625225 21.956747152391284 80-81 21.534052490488516 28.172616141424005 28.15699243687415 22.136338931213327 82-83 21.4211768000538 28.20078910253635 28.310651778797038 22.067382318612815 84-85 21.441941941757516 28.112493602288403 28.14943359295115 22.296130863002933 86-87 21.578556584028536 28.17947999882086 28.13537275594729 22.106590661203313 88-89 21.400605730879583 28.095352409244 28.183785654027403 22.320256205849013 90-91 21.359155868895417 28.00780844116366 28.26740815906935 22.365627530871567 92-93 21.551333600467945 28.15705672925907 28.104735191300623 22.18687447897236 94-95 21.29961637599022 27.928465351049226 28.272369178096195 22.49954909486436 96-97 21.367391304347827 27.900497420781136 28.27166543846721 22.46044583640383 98-99 21.34798465156141 28.02307792641327 28.20002932916535 22.42890809285997 100 21.461124459429932 27.87224768453442 28.121691961741067 22.54493589429458 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2861.0 1 1828.0 2 696.5 3 575.5 4 446.5 5 334.5 6 304.5 7 301.5 8 340.5 9 336.0 10 300.5 11 292.0 12 313.0 13 339.0 14 378.5 15 445.5 16 576.0 17 784.5 18 1053.5 19 1400.5 20 2029.5 21 3012.0 22 4453.5 23 6638.5 24 9409.0 25 12926.5 26 17630.0 27 23145.0 28 30011.5 29 38243.5 30 45941.0 31 53471.0 32 61195.0 33 68612.0 34 75824.0 35 81854.0 36 87050.5 37 92449.5 38 96710.5 39 98728.0 40 99427.0 41 99803.5 42 99395.0 43 98507.5 44 97202.0 45 95143.0 46 94027.5 47 91729.5 48 88884.0 49 86724.5 50 84007.0 51 80807.0 52 74936.5 53 70006.0 54 66856.5 55 62794.5 56 58055.5 57 52611.5 58 47459.0 59 43493.5 60 38714.5 61 32641.5 62 27398.0 63 22886.5 64 18749.5 65 15003.0 66 11797.0 67 8915.5 68 6680.0 69 5182.0 70 3803.0 71 2784.5 72 2226.5 73 1633.5 74 1165.0 75 823.0 76 528.5 77 356.5 78 270.5 79 185.5 80 119.0 81 79.5 82 64.0 83 46.0 84 30.0 85 29.5 86 25.5 87 22.5 88 18.0 89 11.5 90 10.5 91 10.0 92 9.0 93 7.0 94 4.0 95 3.0 96 1.5 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07456039741980523 2 0.07691687417776451 3 0.07791101281002857 4 0.07975201027718426 5 0.07831603225280283 6 0.07813193250608726 7 0.07739553351922498 8 0.07735871356988187 9 0.07702733402579386 10-11 0.07686164425374983 12-13 0.07592273554550043 14-15 0.0746156273438199 16-17 0.07317964931943848 18-19 0.07271939995264955 20-21 0.07238802040856153 22-23 0.07398968820498697 24-25 0.07389763833162918 26-27 0.07351102886352649 28-29 0.06966334415717111 30-31 0.07459721736914834 32-33 0.07275621990199266 34-35 0.07041815311870495 36-37 0.0675830170192852 38-39 0.06907422496768129 40-41 0.07152275159899835 42-43 0.07618047519090222 44-45 0.07699051407645073 46-47 0.08013861974528695 48-49 0.08371015483156899 50-51 0.08450178374244594 52-53 0.08518295280529353 54-55 0.08496203310923485 56-57 0.08266078627529025 58-59 0.08146413792163905 60-61 0.07929176091039535 62-63 0.07965996040382647 64-65 0.07907084121433665 66-67 0.07850013199951839 68-69 0.07800306268338636 70-71 0.07605160536820132 72-73 0.07618047519090222 74-75 0.07538884628002529 76-77 0.07549930612805462 78-79 0.07595955549484355 80-81 0.07710097392448007 82-83 0.07645662481097559 84-85 0.07577545574812798 86-87 0.07654867468433336 88-89 0.07391604830630075 90-91 0.07117296208023877 92-93 0.0711177321562241 94-95 0.07119137205491033 96-97 0.07065748278943518 98-99 0.06984744390388668 100 0.06944242446111243 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2715919.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.82398806916092 #Duplication Level Percentage of deduplicated Percentage of total 1 79.15105248374358 53.68340039318953 2 13.171241324719524 17.866522289276322 3 3.7573360762768355 7.645125516276839 4 1.470814678314654 3.9902606877583935 5 0.7556527881281428 2.5625692843215666 6 0.41934727207266037 1.7065082626734773 7 0.28651248976273547 1.360269378113335 8 0.18230420413940507 0.9891678533207108 9 0.13197794733596924 0.8056143652956409 >10 0.6410073927044099 7.48275990037524 >50 0.026845417509668103 1.2315534368849466 >100 0.005799287765086888 0.5561464777914311 >500 0.0 0.0 >1k 1.0863752723538031E-4 0.12010215472253188 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 3.681994934311369E-5 0.0 0.0 1.1045984802934108E-4 0.0 10-11 3.681994934311369E-5 0.0 0.0 1.2886982270089793E-4 0.0 12-13 3.681994934311369E-5 0.0 0.0 1.4727979737245476E-4 0.0 14-15 3.681994934311369E-5 0.0 0.0 1.4727979737245476E-4 0.0 16-17 3.681994934311369E-5 1.8409974671556845E-5 0.0 1.4727979737245476E-4 0.0 18-19 3.681994934311369E-5 3.681994934311369E-5 0.0 1.4727979737245476E-4 0.0 20-21 3.681994934311369E-5 3.681994934311369E-5 0.0 1.6568977204401163E-4 0.0 22-23 3.681994934311369E-5 3.681994934311369E-5 0.0 2.025097213871253E-4 0.0 24-25 3.681994934311369E-5 3.681994934311369E-5 0.0 2.945595947449095E-4 0.0 26-27 3.681994934311369E-5 3.681994934311369E-5 0.0 3.8660946810269376E-4 0.0 28-29 3.681994934311369E-5 3.681994934311369E-5 0.0 0.0010125486069356266 0.0 30-31 3.681994934311369E-5 3.681994934311369E-5 0.0 0.004510443794531427 0.0 32-33 3.681994934311369E-5 3.681994934311369E-5 0.0 0.01285016232074668 0.0 34-35 7.363989868622738E-5 3.681994934311369E-5 0.0 0.025571454818792458 0.0 36-37 7.363989868622738E-5 3.681994934311369E-5 0.0 0.04714794513385709 0.0 38-39 7.363989868622738E-5 3.681994934311369E-5 0.0 0.08661893082967496 3.681994934311369E-5 40-41 7.363989868622738E-5 7.363989868622738E-5 0.0 0.1316129089269599 3.681994934311369E-5 42-43 7.363989868622738E-5 1.8409974671556846E-4 0.0 0.18001273233848286 3.681994934311369E-5 44-45 7.363989868622738E-5 1.8409974671556846E-4 0.0 0.236420894732133 3.681994934311369E-5 46-47 7.363989868622738E-5 1.8409974671556846E-4 0.0 0.3065076683067499 3.681994934311369E-5 48-49 7.363989868622738E-5 1.8409974671556846E-4 0.0 0.3852103100276555 3.681994934311369E-5 50-51 7.363989868622738E-5 1.8409974671556846E-4 0.0 0.47512462632353913 3.681994934311369E-5 52-53 7.363989868622738E-5 1.8409974671556846E-4 0.0 0.5662908208970886 3.681994934311369E-5 54-55 7.363989868622738E-5 1.8409974671556846E-4 0.0 0.6607339909621752 3.681994934311369E-5 56-57 7.363989868622738E-5 1.8409974671556846E-4 0.0 0.7725009471931968 3.681994934311369E-5 58-59 7.363989868622738E-5 2.2091969605868216E-4 0.0 0.9053289144484795 3.681994934311369E-5 60-61 7.363989868622738E-5 2.2091969605868216E-4 0.0 1.0512279637205677 3.681994934311369E-5 62-63 9.204987335778423E-5 2.2091969605868216E-4 0.0 1.200219888737477 3.681994934311369E-5 64-65 1.1045984802934108E-4 2.2091969605868216E-4 0.0 1.3549004959278976 3.681994934311369E-5 66-67 1.1045984802934108E-4 2.2091969605868216E-4 0.0 1.5299057151557172 3.681994934311369E-5 68-69 1.1045984802934108E-4 2.2091969605868216E-4 0.0 1.7334832150737927 3.681994934311369E-5 70-71 1.1045984802934108E-4 2.3932967073023902E-4 0.0 1.9551761300686803 3.681994934311369E-5 72-73 1.1045984802934108E-4 2.761496200733527E-4 0.0 2.185300813463141 3.681994934311369E-5 74-75 1.4727979737245476E-4 2.945595947449095E-4 0.0 2.4099209144308062 3.681994934311369E-5 76-77 1.4727979737245476E-4 2.945595947449095E-4 0.0 2.651827981615063 3.681994934311369E-5 78-79 1.4727979737245476E-4 3.3137954408802325E-4 0.0 2.934034483355358 3.681994934311369E-5 80-81 1.4727979737245476E-4 3.497895187595801E-4 0.0 3.2435245675589 3.681994934311369E-5 82-83 1.4727979737245476E-4 3.681994934311369E-4 0.0 3.5696020389415146 3.681994934311369E-5 84-85 1.4727979737245476E-4 4.418393921173643E-4 0.0 3.882497968459295 3.681994934311369E-5 86-87 1.4727979737245476E-4 4.418393921173643E-4 0.0 4.208023140601763 3.681994934311369E-5 88 1.4727979737245476E-4 4.418393921173643E-4 0.0 4.474986183314009 3.681994934311369E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2210 0.0 39.55781 1 GTATCAA 3720 0.0 35.25111 1 TCAACGC 3670 0.0 33.682262 4 ATCAACG 3775 0.0 32.86991 3 CAACGCA 3815 0.0 32.402668 5 AACGCAG 4015 0.0 30.670954 6 TATCAAC 4400 0.0 29.05546 2 GTACATG 8405 0.0 26.674252 1 TACATGG 8425 0.0 25.439377 2 ACATGGG 8355 0.0 24.133615 3 ACGCAGA 5085 0.0 23.84736 7 CGCAGAG 5195 0.0 22.889618 8 GAGTACA 6090 0.0 21.764233 1 CATGGGG 5495 0.0 19.67305 4 TACACCG 335 4.0017767E-11 19.642775 5 GTGTAGG 1110 0.0 19.054655 1 GCAGAGT 6200 0.0 18.345402 9 AGTACAT 6495 0.0 18.019045 2 TATACTG 1760 0.0 17.358824 5 CGTATAC 190 2.0236606E-4 17.316336 3 >>END_MODULE