FastQCFastQC Report
Wed 25 May 2016
SRR1032986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032986_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2715919
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63880.2352058364038103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50930.18752400200447805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30090.1107912275734291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40250.051.7075771
GTATCAA74950.043.940271
ATCAACG88200.036.1041033
TCAACGC88400.036.0224234
CAACGCA90100.035.282745
TATCAAC93900.034.2130362
AACGCAG94400.033.924666
ACGCAGA111100.028.6559627
CGCAGAG112850.028.2115868
GTGGTAT19500.026.0198921
GCAGAGT122650.025.6970569
GTACATG78000.025.2368871
TACATGG79600.023.3410572
TGGTATC20400.022.8266142
ACATGGG78900.022.4154423
ATAACGC2607.075869E-1021.7092293
TATACCG2257.960625E-820.9005585
GAGTACT83550.020.77286512-13
CAGAGTA121350.020.7469110-11
GTCTAGG8200.020.6255251
Produced by FastQC (version 0.11.5)