FastQCFastQC Report
Wed 25 May 2016
SRR1032985_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032985_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265740
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19550.039.6689451
GTATCAA34450.036.018711
TCAACGC34850.033.9876254
ATCAACG35850.033.301063
CAACGCA36350.032.584395
AACGCAG38050.031.128586
TATCAAC42850.028.1900442
GTACATG74400.027.7335241
ACGCAGA45250.025.9677857
TACATGG74800.025.888632
CGCAGAG47250.024.569118
ACATGGG77150.024.4908353
CATGGGG51750.021.3442344
GCAGAGT57050.020.2662689
GAGTACA54500.019.4906081
GTATAGG9200.018.9028531
AGTACAT55550.018.1068632
CTGTCGC5550.017.7836389
AGAGTAC48100.016.75728810-11
CCTATAC8350.016.323943
Produced by FastQC (version 0.11.5)