##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032985_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2265740
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.438523837686585	33.0	31.0	34.0	30.0	34.0
2	31.453954999249692	33.0	31.0	34.0	28.0	34.0
3	31.622896272299556	34.0	31.0	34.0	30.0	34.0
4	34.989999293828944	37.0	35.0	37.0	33.0	37.0
5	35.04963720462189	37.0	35.0	37.0	33.0	37.0
6	35.1322349431091	37.0	35.0	37.0	33.0	37.0
7	35.02728115317733	37.0	35.0	37.0	33.0	37.0
8	35.024650224650664	37.0	35.0	37.0	33.0	37.0
9	36.63832964064721	39.0	38.0	39.0	33.0	39.0
10-11	36.667568432388535	39.0	38.0	39.0	33.0	39.0
12-13	36.632100549930705	39.0	38.0	39.0	33.0	39.0
14-15	38.068804452408486	41.0	39.0	41.0	33.0	41.0
16-17	38.07665244026234	41.0	39.0	41.0	33.0	41.0
18-19	38.071390141852106	41.0	39.0	41.0	33.5	41.0
20-21	38.131127799306185	41.0	39.0	41.0	34.0	41.0
22-23	38.10887855623328	41.0	39.0	41.0	33.0	41.0
24-25	38.14314727197295	41.0	39.0	41.0	33.0	41.0
26-27	38.129486392966534	41.0	39.0	41.0	33.0	41.0
28-29	38.113832566843506	41.0	39.0	41.0	33.0	41.0
30-31	38.06445488008333	41.0	39.0	41.0	33.0	41.0
32-33	38.02181384448348	41.0	38.5	41.0	33.0	41.0
34-35	37.94641618190966	41.0	38.0	41.0	33.0	41.0
36-37	37.844199025483945	40.0	38.0	41.0	33.0	41.0
38-39	37.74272379002004	40.0	38.0	41.0	32.0	41.0
40-41	37.661078279061144	40.0	38.0	41.0	32.0	41.0
42-43	37.524224756591664	40.0	38.0	41.0	32.0	41.0
44-45	37.36793233115891	40.0	38.0	41.0	31.0	41.0
46-47	37.23665116032731	40.0	38.0	41.0	31.0	41.0
48-49	37.09781660737772	40.0	37.0	41.0	31.0	41.0
50-51	36.95272913043862	40.0	37.0	41.0	30.5	41.0
52-53	36.79595871547485	40.0	36.5	41.0	30.0	41.0
54-55	36.629401431761806	40.0	36.0	41.0	30.0	41.0
56-57	36.44428023515496	40.0	36.0	41.0	30.0	41.0
58-59	36.2360039104222	39.0	35.0	41.0	30.0	41.0
60-61	35.99213766804664	39.0	35.0	41.0	29.5	41.0
62-63	35.73776227634239	39.0	35.0	40.5	29.0	41.0
64-65	35.457188600633785	38.0	35.0	40.0	29.0	41.0
66-67	35.16525704626304	37.5	34.5	40.0	29.0	41.0
68-69	34.86088033048805	37.0	34.0	40.0	28.0	41.0
70-71	34.52236267179818	36.5	34.0	39.5	28.0	41.0
72-73	34.23429409376186	36.0	34.0	39.0	28.0	41.0
74-75	33.96787672018854	36.0	34.0	39.0	28.0	40.5
76-77	33.64623213607916	35.0	34.0	38.0	28.0	40.0
78-79	33.2754283368789	35.0	34.0	37.0	27.0	39.0
80-81	32.94712323567576	35.0	33.5	37.0	27.0	39.0
82-83	32.62747446750289	35.0	33.0	36.0	26.0	38.5
84-85	32.46511029509123	35.0	33.0	36.0	26.5	37.0
86-87	32.233476038733485	35.0	33.0	36.0	26.0	37.0
88-89	31.995649324282574	35.0	33.0	35.0	26.0	37.0
90-91	31.787014838419235	35.0	33.0	35.0	25.5	36.0
92-93	31.591565890172745	35.0	33.0	35.0	25.0	36.0
94-95	31.404336772974833	35.0	33.0	35.0	25.0	36.0
96-97	31.218483144579693	35.0	33.0	35.0	24.5	35.5
98-99	31.025874990069468	35.0	33.0	35.0	23.5	35.0
100	30.887897993591498	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5406738494966916
1101	2	0.5568588213060011
1101	3	0.5165689583227007
1101	4	0.49860376388479466
1101	5	0.4097324075979003
1101	6	0.4720597655667689
1101	7	0.45254670968255795
1101	8	0.37355055527115866
1101	9	0.5013794369530231
1101	10-11	0.4802071872008895
1101	12-13	0.23701367524317618
1101	14-15	0.28725406358906014
1101	16-17	0.3469255441848915
1101	18-19	0.3576968546071271
1101	20-21	0.3205324301702248
1101	22-23	0.42233693554873497
1101	24-25	0.41157031984437253
1101	26-27	0.4799149857014129
1101	28-29	0.4432787781483256
1101	30-31	0.42017568665868765
1101	32-33	0.4667406464677555
1101	34-35	0.5354649584875304
1101	36-37	0.47573162734723695
1101	38-39	0.4311507962649159
1101	40-41	0.5071219012617547
1101	42-43	0.46099919766277964
1101	44-45	0.4925796256360684
1101	46-47	0.44411690237536305
1101	48-49	0.4519181928464775
1101	50-51	0.4588392063776041
1101	52-53	0.46084786141867795
1101	54-55	0.4658640983813882
1101	56-57	0.38148661349419655
1101	58-59	0.46570939576749737
1101	60-61	0.49538112583491767
1101	62-63	0.5301800487238069
1101	64-65	0.5199182042271602
1101	66-67	0.472245487526294
1101	68-69	0.4471220999621295
1101	70-71	0.42835741762709745
1101	72-73	0.36923901686655825
1101	74-75	0.39239060837311257
1101	76-77	0.3555030451621306
1101	78-79	0.528478791507851
1101	80-81	0.5447709189303325
1101	82-83	0.540710697717202
1101	84-85	0.47917340057107083
1101	86-87	0.38267114059686236
1101	88-89	0.4260430227977494
1101	90-91	0.5112495615602803
1101	92-93	0.47283133775508546
1101	94-95	0.4779711380137819
1101	96-97	0.44626282106353443
1101	98-99	0.4492691205744883
1101	100	0.4782965864176809
1102	1	0.5376197395628779
1102	2	0.4568101485739646
1102	3	0.5331632399006949
1102	4	0.5327383252685962
1102	5	0.5214820883130997
1102	6	0.48408380240827853
1102	7	0.42593333341738315
1102	8	0.414825032039289
1102	9	0.5004873115354513
1102	10-11	0.5096423339054894
1102	12-13	0.5455776840458668
1102	14-15	0.6585558719682538
1102	16-17	0.6376115801028632
1102	18-19	0.5562435367492284
1102	20-21	0.6574343691753839
1102	22-23	0.6712296308948709
1102	24-25	0.6589360642251734
1102	26-27	0.6172471791775962
1102	28-29	0.6479217199781644
1102	30-31	0.6524088914460862
1102	32-33	0.6766610436705278
1102	34-35	0.664903017235936
1102	36-37	0.7029052600386763
1102	38-39	0.7498147089701561
1102	40-41	0.7493573524829458
1102	42-43	0.7242659491947236
1102	44-45	0.7155028184008785
1102	46-47	0.7078170284252536
1102	48-49	0.7462640438080683
1102	50-51	0.744974653854733
1102	52-53	0.7218942835568711
1102	54-55	0.6903674909124149
1102	56-57	0.746513903504642
1102	58-59	0.6725480186203825
1102	60-61	0.8521645238572191
1102	62-63	0.817643388172435
1102	64-65	0.7928569972266146
1102	66-67	0.7523192038349862
1102	68-69	0.7992795084095476
1102	70-71	0.769922994773367
1102	72-73	0.7763121632466721
1102	74-75	0.7592371977458257
1102	76-77	0.7762241088069288
1102	78-79	0.8168630648073218
1102	80-81	0.8083876357257509
1102	82-83	0.6912775062420025
1102	84-85	0.6562877523709147
1102	86-87	0.6869716052758577
1102	88-89	0.690876661895679
1102	90-91	0.644945028340107
1102	92-93	0.5717426493812177
1102	94-95	0.6293794231943934
1102	96-97	0.5776902217316398
1102	98-99	0.6317889536727783
1102	100	0.5674184388103427
1103	1	0.28123846686309406
1103	2	0.35322796611355756
1103	3	0.4070437324839631
1103	4	0.2653340865818947
1103	5	0.21892636225784656
1103	6	0.23142376592777936
1103	7	0.24684351224904333
1103	8	0.26951827735617684
1103	9	0.3819952526390722
1103	10-11	0.382271222022311
1103	12-13	0.4438054523335637
1103	14-15	0.4962013596777979
1103	16-17	0.47390835090302375
1103	18-19	0.42946957591268387
1103	20-21	0.36632826046091793
1103	22-23	0.4472129396183462
1103	24-25	0.4162345770174767
1103	26-27	0.428786509918055
1103	28-29	0.41240112490207537
1103	30-31	0.41906638973304666
1103	32-33	0.4953366310098062
1103	34-35	0.5138024899736777
1103	36-37	0.4807634621998389
1103	38-39	0.4796235643381621
1103	40-41	0.5190928929830534
1103	42-43	0.47704420951880167
1103	44-45	0.5272231040039514
1103	46-47	0.5422131699850397
1103	48-49	0.49028288241564155
1103	50-51	0.44226672465392625
1103	52-53	0.4444665529077838
1103	54-55	0.4832288617942879
1103	56-57	0.4640013412159547
1103	58-59	0.4666477239454423
1103	60-61	0.43852442779833467
1103	62-63	0.4868283433438805
1103	64-65	0.3944331945044084
1103	66-67	0.4035916382167244
1103	68-69	0.37851501834839496
1103	70-71	0.3672948063486956
1103	72-73	0.41540191203307586
1103	74-75	0.4060623647872674
1103	76-77	0.26581337343853306
1103	78-79	0.2710961620146719
1103	80-81	0.3241375448462023
1103	82-83	0.2959667303397211
1103	84-85	0.2666040695132139
1103	86-87	0.22041246600977615
1103	88-89	0.2948557386880992
1103	90-91	0.2820752370252926
1103	92-93	0.32411396232535594
1103	94-95	0.3002945079134349
1103	96-97	0.33626366206157243
1103	98-99	0.39868370389072894
1103	100	0.3916372767745102
1104	1	0.2818612100974498
1104	2	0.32421573149678906
1104	3	0.28145278560403497
1104	4	0.21839103462820475
1104	5	0.19821762927277575
1104	6	0.2214606496989191
1104	7	0.24445413254162673
1104	8	0.20402864451876468
1104	9	0.3305479756478391
1104	10-11	0.29398825618244473
1104	12-13	0.31138798326133355
1104	14-15	0.32650537935741397
1104	16-17	0.269042888258312
1104	18-19	0.33720275951879586
1104	20-21	0.3950620830962066
1104	22-23	0.34559334189651025
1104	24-25	0.28381633242440785
1104	26-27	0.3977559823157151
1104	28-29	0.41933660777236526
1104	30-31	0.370885218758616
1104	32-33	0.3623080515158179
1104	34-35	0.4026447614431916
1104	36-37	0.3903311403524867
1104	38-39	0.38068726872203484
1104	40-41	0.4173410689580379
1104	42-43	0.32092372149904946
1104	44-45	0.34217336209728444
1104	46-47	0.33208153307769095
1104	48-49	0.2758377985973439
1104	50-51	0.32608439660240407
1104	52-53	0.2880831633233356
1104	54-55	0.2833534879932387
1104	56-57	0.3360504844205323
1104	58-59	0.32888924284953447
1104	60-61	0.3509215937349808
1104	62-63	0.3487565518286928
1104	64-65	0.312983342783987
1104	66-67	0.43350192596597736
1104	68-69	0.3479362316352379
1104	70-71	0.2881393330328166
1104	72-73	0.40319366297300263
1104	74-75	0.4034570025259683
1104	76-77	0.3700197209215048
1104	78-79	0.35347818978451784
1104	80-81	0.39972019365313827
1104	82-83	0.33243559783112886
1104	84-85	0.35975769398741164
1104	86-87	0.3114128568722805
1104	88-89	0.3109334489783322
1104	90-91	0.3460545430272113
1104	92-93	0.2912737568433563
1104	94-95	0.33360196470141545
1104	96-97	0.35408823304344494
1104	98-99	0.3575803238606987
1104	100	0.3729169792226763
1105	1	0.20420443544553635
1105	2	0.22451067891920218
1105	3	0.21633698815523417
1105	4	0.20453303832499614
1105	5	0.1757407858145399
1105	6	0.14184087539781842
1105	7	0.12282314404767192
1105	8	0.13547547880872202
1105	9	0.19535250112298286
1105	10-11	0.1904102683105222
1105	12-13	0.20972131290966445
1105	14-15	0.2843993685323625
1105	16-17	0.25520239570643355
1105	18-19	0.31871456036369494
1105	20-21	0.28255589229573275
1105	22-23	0.31677277045614716
1105	24-25	0.3212287583927491
1105	26-27	0.3595304610355754
1105	28-29	0.31686780498473865
1105	30-31	0.33307020650968155
1105	32-33	0.33963415266759966
1105	34-35	0.35919326489088377
1105	36-37	0.4017471790951035
1105	38-39	0.3474992349497441
1105	40-41	0.2962251513782377
1105	42-43	0.3347468024246041
1105	44-45	0.31995824578393695
1105	46-47	0.35195262071289335
1105	48-49	0.35192209150474696
1105	50-51	0.3762101656619663
1105	52-53	0.33657656938383695
1105	54-55	0.37348334371810665
1105	56-57	0.39784830761716705
1105	58-59	0.4417168577994346
1105	60-61	0.38344411645669396
1105	62-63	0.44479641100272715
1105	64-65	0.4327994686400203
1105	66-67	0.4213773537945116
1105	68-69	0.4818043690592191
1105	70-71	0.4283481583136819
1105	72-73	0.4367244017713432
1105	74-75	0.35818797171052097
1105	76-77	0.3646677186397511
1105	78-79	0.3705074973287452
1105	80-81	0.35649818049326853
1105	82-83	0.29292815707856334
1105	84-85	0.31519212972542476
1105	86-87	0.30644087024440836
1105	88-89	0.28708256104066265
1105	90-91	0.3283366010556428
1105	92-93	0.34023709256457124
1105	94-95	0.3246117616431583
1105	96-97	0.37440382383742943
1105	98-99	0.3080043568429254
1105	100	0.22620329932243521
1106	1	0.1700398787231059
1106	2	0.14097811484208478
1106	3	0.10608503843585737
1106	4	0.15186845869434507
1106	5	0.10896385897242311
1106	6	0.06839226153963551
1106	7	0.09241341242408652
1106	8	0.11343131565479325
1106	9	0.20651057260830186
1106	10-11	0.17851011205701184
1106	12-13	0.2165805554383553
1106	14-15	0.27066306105771787
1106	16-17	0.26319413565729377
1106	18-19	0.23377787284258744
1106	20-21	0.2672864146933378
1106	22-23	0.2526411679581386
1106	24-25	0.26996379132132375
1106	26-27	0.3290560733970267
1106	28-29	0.3161333268065363
1106	30-31	0.3265144315423356
1106	32-33	0.3367397125011209
1106	34-35	0.29318000430485114
1106	36-37	0.3063193234441286
1106	38-39	0.2963750782419581
1106	40-41	0.3431296849589387
1106	42-43	0.33924769248408637
1106	44-45	0.2889746588592743
1106	46-47	0.2667116253675985
1106	48-49	0.36874114647262246
1106	50-51	0.2523544931005475
1106	52-53	0.34764181393659044
1106	54-55	0.43164779748622095
1106	56-57	0.3700041195914636
1106	58-59	0.28947104748267094
1106	60-61	0.3948951743299318
1106	62-63	0.3175438653158622
1106	64-65	0.3057866632111015
1106	66-67	0.351748530620668
1106	68-69	0.3472610395344873
1106	70-71	0.2985418327088212
1106	72-73	0.2475218855113681
1106	74-75	0.3144967801594305
1106	76-77	0.2850873529821314
1106	78-79	0.317422167132861
1106	80-81	0.28053076818618905
1106	82-83	0.26956155287438577
1106	84-85	0.31426695610500843
1106	86-87	0.3201055342551413
1106	88-89	0.28131632358025627
1106	90-91	0.2739282548928266
1106	92-93	0.25633777586631723
1106	94-95	0.3018722588700271
1106	96-97	0.2898945647599831
1106	98-99	0.2880723049192824
1106	100	0.33918822606831966
1107	1	0.3127405953157343
1107	2	0.2426781838709111
1107	3	0.2285954802856196
1107	4	0.22879164647149253
1107	5	0.2802514784324259
1107	6	0.21694131256917615
1107	7	0.23012036856819407
1107	8	0.17925877444540106
1107	9	0.19758675515398494
1107	10-11	0.16968560354301587
1107	12-13	0.2937875557056415
1107	14-15	0.35817415860927326
1107	16-17	0.3215374811232934
1107	18-19	0.35746883223645654
1107	20-21	0.28434852139412214
1107	22-23	0.3764767886466487
1107	24-25	0.39564270970751636
1107	26-27	0.4072389969894985
1107	28-29	0.3559622305994452
1107	30-31	0.3995866958141434
1107	32-33	0.24366218566079567
1107	34-35	0.2749688300103301
1107	36-37	0.3910265010942169
1107	38-39	0.35844799787648896
1107	40-41	0.32335249810867595
1107	42-43	0.3477696965563055
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2214	100	-0.32630902938242556
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2215	4	-0.39020919057229264
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2215	26-27	-0.5860053509053813
2215	28-29	-0.6731651672369665
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2215	100	-0.8602153998754005
2216	1	-0.8734969164582225
2216	2	-0.8435153392876202
2216	3	-0.8795239254919558
2216	4	-0.7633815869474105
2216	5	-0.6231199500418825
2216	6	-0.5961190425747915
2216	7	-0.651086206713785
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2216	10-11	-0.9244594548462715
2216	12-13	-0.9642412902741313
2216	14-15	-1.1853299022278705
2216	16-17	-1.1746595689571464
2216	18-19	-1.1970325671897015
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2216	24-25	-1.3247760917641642
2216	26-27	-1.2838517824927607
2216	28-29	-1.3773414740281993
2216	30-31	-1.4412276086067237
2216	32-33	-1.472482596811325
2216	34-35	-1.4382660386781438
2216	36-37	-1.5483171293535278
2216	38-39	-1.5877838448120087
2216	40-41	-1.5433280062891868
2216	42-43	-1.5634967388591647
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2216	50-51	-1.4744444682856468
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2216	54-55	-1.4973965710286734
2216	56-57	-1.50140523327957
2216	58-59	-1.4576876433447907
2216	60-61	-1.5225250352253568
2216	62-63	-1.547209673358303
2216	64-65	-1.6250800626153037
2216	66-67	-1.6585226747476156
2216	68-69	-1.5757888157409496
2216	70-71	-1.5271835247573762
2216	72-73	-1.565314101264292
2216	74-75	-1.530231126009788
2216	76-77	-1.495635341381515
2216	78-79	-1.4778296501023647
2216	80-81	-1.4621479466362324
2216	82-83	-1.4879274852958417
2216	84-85	-1.5835924166945432
2216	86-87	-1.5103830942245757
2216	88-89	-1.4555275956079683
2216	90-91	-1.5753699587453518
2216	92-93	-1.5425792443256618
2216	94-95	-1.5667133979406351
2216	96-97	-1.6469812217844826
2216	98-99	-1.6842458236077675
2216	100	-1.7214975329326663
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33897.0
3	5733.0
4	3870.0
5	3136.0
6	2515.0
7	2804.0
8	3271.0
9	3373.0
10	3503.0
11	3675.0
12	3938.0
13	4496.0
14	4789.0
15	4864.0
16	4811.0
17	4815.0
18	4896.0
19	4774.0
20	4738.0
21	4922.0
22	6090.0
23	8945.0
24	14107.0
25	17509.0
26	16967.0
27	14832.0
28	16407.0
29	20164.0
30	25220.0
31	31039.0
32	40044.0
33	53823.0
34	78689.0
35	123951.0
36	233182.0
37	472122.0
38	712938.0
39	266637.0
40	254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.06683698122741	19.958914262342724	13.501536629556114	23.472712126873745
2	15.343282474543734	20.40502990086931	40.43549835444732	23.816189270139635
3	18.102206933350296	25.910750915653836	29.889229295975717	26.097812855020152
4	12.280821094887417	17.085397614033106	38.97325151596534	31.66052977511414
5	12.878183878141916	38.49625322169929	35.15460843792583	13.470954462232962
6	30.429501345214625	38.38068933470733	18.013817436861487	13.175991883216554
7	27.382956346901427	32.853849643302475	22.40597571603423	17.357218293761868
8	24.92287890486278	37.02775476021788	20.292079584023124	17.757286750896213
9	24.96851759503137	17.91385283240053	20.971455805593997	36.1461737669741
10-11	23.68201829262098	27.685555437277742	28.56767726470236	20.064749005398916
12-13	24.29449489476252	25.00849713460499	28.670579949589186	22.0264280210433
14-15	21.78685794799539	26.82985649483443	25.65540209272845	25.727883464441724
16-17	21.543496057858853	30.166561288198224	26.186324885609334	22.10361776833359
18-19	21.568614460345717	29.110239084447088	28.022863205252303	21.298283249954896
20-21	22.66527390234234	28.305549819849762	27.61074298308476	21.418433294723137
22-23	22.395499191157388	28.00786206058866	27.607780348161192	21.988858400092752
24-25	22.128525098469215	28.116601683058878	27.65536713806008	22.09950608041183
26-27	21.973081890345274	28.048936155176644	27.741522721209584	22.236459233268494
28-29	22.02542016365983	28.144676970029558	27.759940551576257	22.069962314734354
30-31	22.183640441656486	28.494003150146067	27.41055519778518	21.911801210412264
32-33	21.904754333032546	28.197274720071587	27.846864550027583	22.051106396868285
34-35	22.052298802473096	27.9324966449504	27.91971024561591	22.095494306960596
36-37	22.304191162944868	28.29240909997308	27.411614101111446	21.991785635970604
38-39	22.112542468699864	28.427115043387914	27.5682563237923	21.892086164119924
40-41	21.967497453181718	28.0958721116276	27.836784558275816	22.099845876914863
42-43	21.897232625452354	28.069507466253118	27.75413463063171	22.279125277662814
44-45	21.986858927711292	28.207545107146935	27.792897478125266	22.012698487016504
46-47	22.02648980294979	28.285981209334377	27.64054348930381	22.046985498412024
48-49	21.89426167574909	28.17419606057146	27.751986643728888	22.179555619950566
50-51	21.932235524445375	28.067565693163033	27.719586073217293	22.280612709174292
52-53	21.99268965904144	28.01697338455446	27.764607816264792	22.22572914013931
54-55	22.032394896594525	28.336202793333726	27.592712639133836	22.03868967093791
56-57	21.83904948798111	28.14307153908251	27.707549034656935	22.310329938279445
58-59	21.838182287212614	27.741119576510165	27.97578556233259	22.444912573944634
60-61	22.099087065375446	28.197148470697574	27.54553188557132	22.15823257835566
62-63	21.86297838544347	28.192987206539538	27.60258775709608	22.341446650920915
64-65	21.8896020845347	27.86313769912724	27.828661954613487	22.41859826172457
66-67	21.759024702673454	27.935134776991287	27.707477058648145	22.598363461687114
68-69	22.14496977385383	28.044832579041618	27.62017355629625	22.1900240908083
70-71	22.05803561766422	27.839114942696273	27.69410540422237	22.408744035417133
72-73	21.770623618032296	28.000487636385802	27.891564508905102	22.337324236676793
74-75	21.865092870146107	27.973787038793603	27.771158454669393	22.3899616363909
76-77	21.933065797938557	27.982227911269415	27.590531711689863	22.49417457910216
78-79	21.983962346031962	27.98042566104141	27.732610360185774	22.30300163274086
80-81	21.799884847865126	27.897978830470393	27.874259345841352	22.42787697582313
82-83	21.7978997117901	27.834671319250432	28.003224417230754	22.364204551728708
84-85	21.759434097690146	27.870114554139917	27.813621496513907	22.556829851656033
86-87	21.95256938993746	27.908781036589968	27.78426585735481	22.35438371611776
88-89	21.693569456281043	27.843045626492213	27.82074043090546	22.642644486321284
90-91	21.69183102448418	27.73790200586101	27.919805310242634	22.650461659412176
92-93	21.87342998775018	27.86389557595254	27.756546764750635	22.506127671546647
94-95	21.667650868932085	27.64083846082838	27.89436071977354	22.797149950465993
96-97	21.729492372536626	27.59300408414568	27.92721767078703	22.750285872530664
98-99	21.671465769430625	27.704776010831495	27.840456756510257	22.783301463227627
100	21.787074140994513	27.56807273694118	27.805909390639084	22.838943731425225
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2586.0
1	1676.0
2	672.5
3	478.5
4	329.0
5	283.5
6	271.0
7	258.5
8	249.0
9	265.5
10	278.0
11	273.0
12	292.5
13	295.0
14	330.5
15	426.5
16	534.0
17	648.0
18	854.5
19	1145.5
20	1536.5
21	2189.5
22	3204.5
23	4673.5
24	6698.0
25	9301.5
26	12633.5
27	16702.0
28	21853.5
29	28049.5
30	34526.5
31	41032.5
32	47332.5
33	53758.5
34	59924.5
35	64939.0
36	69939.0
37	74651.5
38	78476.0
39	80114.5
40	81392.5
41	83227.5
42	83148.0
43	82660.0
44	81581.5
45	80507.0
46	79646.0
47	77331.5
48	75118.5
49	73266.5
50	70851.5
51	68346.0
52	64336.5
53	60396.0
54	58110.0
55	55304.5
56	50922.5
57	45819.0
58	41525.0
59	38635.5
60	35067.0
61	30219.0
62	25842.5
63	21916.0
64	17970.0
65	14291.0
66	11411.5
67	8853.0
68	6631.0
69	5006.0
70	3638.5
71	2718.5
72	2143.0
73	1559.0
74	1160.0
75	849.5
76	563.5
77	392.5
78	278.5
79	185.5
80	134.5
81	94.0
82	68.5
83	51.5
84	39.5
85	30.5
86	24.0
87	21.5
88	16.0
89	8.0
90	6.5
91	7.5
92	5.5
93	3.5
94	3.0
95	3.5
96	3.0
97	2.5
98	2.0
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07476586016047737
2	0.07763468006037762
3	0.07873807232957003
4	0.08103312824949023
5	0.07922356492801469
6	0.07895875078340851
7	0.07842912249419616
8	0.07803190127728689
9	0.07842912249419616
10-11	0.0779877655865192
12-13	0.07686230547194293
14-15	0.07547203121276051
16-17	0.07408175695357808
18-19	0.07350799297359803
20-21	0.07315490744745647
22-23	0.07514101353200278
24-25	0.07536169198584126
26-27	0.0745010460158712
28-29	0.0702860875475562
30-31	0.07582511673890208
32-33	0.07410382479896192
34-35	0.07130120843521322
36-37	0.06777035317379752
38-39	0.06940337373220228
40-41	0.07240460070440562
42-43	0.07736986591577145
44-45	0.07809810481343843
46-47	0.08165102792023797
48-49	0.08538049379010831
50-51	0.08641768252314917
52-53	0.0865280217500684
54-55	0.08639561467776533
56-57	0.08418883013938051
58-59	0.08315164140633965
60-61	0.08085658548641944
62-63	0.08160689222947028
64-65	0.0807462462595002
66-67	0.08010627874336862
68-69	0.07970905752645935
70-71	0.07728159453423605
72-73	0.07759054436960994
74-75	0.07703884823501372
76-77	0.07681816978117524
78-79	0.07748020514269069
80-81	0.0784953260303477
82-83	0.07789949420498381
84-85	0.07701678038962988
86-87	0.07763468006037762
88-89	0.07474379231509352
90-91	0.07172049749750634
92-93	0.07174256534289018
94-95	0.07233839716825408
96-97	0.07145568335290016
98-99	0.07068330876446546
100	0.0697785271037277
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2265740.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.77759072016711
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.18925866482746	54.3503475393829
2	12.358164540921189	16.752132366140764
3	3.5282893622939606	7.174168570196386
4	1.4136739775681855	3.8326166505346873
5	0.7178048313461571	2.4325541037969205
6	0.4265633551604879	1.7346861901373294
7	0.2962362470021088	1.4054725374051082
8	0.19506126019064055	1.057662580684901
9	0.14279298766412943	0.8710348208048313
>10	0.6955501821886703	8.200970513369823
>50	0.029419382239637474	1.3268157779174403
>100	0.007054874259746925	0.7257194835545064
>500	0.0	0.0
>1k	1.3033433754567204E-4	0.13581886607442803
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	2.2067845383848103E-5	2.2067845383848103E-5	0.0	0.0	0.0
16-17	4.413569076769621E-5	4.413569076769621E-5	0.0	0.0	0.0
18-19	4.413569076769621E-5	4.413569076769621E-5	0.0	2.2067845383848103E-5	0.0
20-21	4.413569076769621E-5	4.413569076769621E-5	0.0	1.3240707230308861E-4	0.0
22-23	4.413569076769621E-5	6.620353615154431E-5	0.0	2.6481414460617723E-4	0.0
24-25	4.413569076769621E-5	1.1033922691924052E-4	0.0	3.3101768075772153E-4	0.0
26-27	4.413569076769621E-5	1.3240707230308861E-4	0.0	3.9722121690926584E-4	0.0
28-29	4.413569076769621E-5	1.3240707230308861E-4	0.0	0.001081324423808557	0.0
30-31	4.413569076769621E-5	1.3240707230308861E-4	0.0	0.005031468747517367	0.0
32-33	4.413569076769621E-5	1.3240707230308861E-4	0.0	0.014344099499501266	0.0
34-35	4.413569076769621E-5	1.3240707230308861E-4	0.0	0.028224774245941726	0.0
36-37	4.413569076769621E-5	1.3240707230308861E-4	0.0	0.05016021255748673	0.0
38-39	4.413569076769621E-5	1.765427630707848E-4	0.0	0.08869067059768553	0.0
40-41	4.413569076769621E-5	3.5308552614156966E-4	0.0	0.138453661938263	0.0
42-43	4.413569076769621E-5	3.5308552614156966E-4	0.0	0.18673810763812265	0.0
44-45	4.413569076769621E-5	3.751533715254178E-4	0.0	0.24301111336693532	0.0
46-47	4.413569076769621E-5	3.9722121690926584E-4	0.0	0.3144226610290678	0.0
48-49	4.413569076769621E-5	3.9722121690926584E-4	0.0	0.3951027037524164	0.0
50-51	8.827138153539241E-5	3.9722121690926584E-4	0.0	0.48540432706312286	0.0
52-53	8.827138153539241E-5	3.9722121690926584E-4	0.0	0.5774272423137694	0.0
54-55	8.827138153539241E-5	3.9722121690926584E-4	0.0	0.6755850185811259	0.0
56-57	8.827138153539241E-5	3.9722121690926584E-4	0.0	0.7923459885070662	0.0
58-59	8.827138153539241E-5	3.9722121690926584E-4	0.0	0.9266950312039334	0.0
60-61	8.827138153539241E-5	3.9722121690926584E-4	0.0	1.0657224571221764	0.0
62-63	8.827138153539241E-5	3.9722121690926584E-4	0.0	1.2091413842718053	0.0
64-65	8.827138153539241E-5	3.9722121690926584E-4	0.0	1.3619832814003372	0.0
66-67	8.827138153539241E-5	3.9722121690926584E-4	0.0	1.5337814577135949	0.0
68-69	8.827138153539241E-5	3.9722121690926584E-4	0.0	1.7286405324529734	0.0
70-71	8.827138153539241E-5	4.4135690767696203E-4	0.0	1.9495838004360606	0.0
72-73	8.827138153539241E-5	4.4135690767696203E-4	0.0	2.1815389232656877	0.0
74-75	8.827138153539241E-5	4.4135690767696203E-4	0.0	2.4159435769329227	0.0
76-77	8.827138153539241E-5	4.4135690767696203E-4	0.0	2.6626400204789604	0.0
78-79	8.827138153539241E-5	4.4135690767696203E-4	0.0	2.9481096683644195	0.0
80-81	8.827138153539241E-5	4.4135690767696203E-4	0.0	3.2508805070308155	0.0
82-83	8.827138153539241E-5	4.4135690767696203E-4	0.0	3.5723428107373305	0.0
84-85	8.827138153539241E-5	4.4135690767696203E-4	0.0	3.8892812061401574	0.0
86-87	8.827138153539241E-5	4.6342475306081015E-4	0.0	4.221865703920132	0.0
88	8.827138153539241E-5	4.8549259844465827E-4	0.0	4.495573190216	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1955	0.0	39.668945	1
GTATCAA	3445	0.0	36.01871	1
TCAACGC	3485	0.0	33.987625	4
ATCAACG	3585	0.0	33.30106	3
CAACGCA	3635	0.0	32.58439	5
AACGCAG	3805	0.0	31.12858	6
TATCAAC	4285	0.0	28.190044	2
GTACATG	7440	0.0	27.733524	1
ACGCAGA	4525	0.0	25.967785	7
TACATGG	7480	0.0	25.88863	2
CGCAGAG	4725	0.0	24.56911	8
ACATGGG	7715	0.0	24.490835	3
CATGGGG	5175	0.0	21.344234	4
GCAGAGT	5705	0.0	20.266268	9
GAGTACA	5450	0.0	19.490608	1
GTATAGG	920	0.0	18.902853	1
AGTACAT	5555	0.0	18.106863	2
CTGTCGC	555	0.0	17.783638	9
AGAGTAC	4810	0.0	16.757288	10-11
CCTATAC	835	0.0	16.32394	3
>>END_MODULE