FastQCFastQC Report
Wed 25 May 2016
SRR1032985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032985_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265740
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55880.24663024000988637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45260.19975813641459306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25310.11170743333303909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34800.052.9221421
GTATCAA63750.044.6604421
ATCAACG78800.035.708163
TCAACGC78750.035.671084
CAACGCA80750.034.7829745
AACGCAG82900.033.8801356
TATCAAC84200.033.5849952
ACGCAGA98150.028.5674637
CGCAGAG99800.027.7651778
GCAGAGT110550.024.9851559
GTACATG76600.024.9629671
TACATGG77700.023.4959852
GTGGTAT16250.023.418671
ACATGGG77800.022.619583
TGGTATC16300.021.9385992
GAGTACT68100.021.55543512-13
AGAGTAC100950.021.03226310-11
CAGAGTA106350.020.71520610-11
AGTACTT71700.020.27661312-13
GTACTTT75450.019.45560314-15
Produced by FastQC (version 0.11.5)