FastQCFastQC Report
Wed 25 May 2016
SRR1032984_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032984_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3459134
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42450.036.2050631
ATCAACG42000.034.2424743
TCAACGC42600.033.7601854
CAACGCA44600.032.2458085
AACGCAG46800.030.529136
GGTATCA26450.030.2080381
TATCAAC52950.028.226722
GTACATG103400.026.3637221
TACATGG102000.025.8500842
TATACCG2008.1490725E-1025.8493355
ACATGGG103100.024.5710143
ACGCAGA58500.023.9412657
CGCAGAG60800.022.649098
ACACCGT6500.020.2456346
CATGGGG72150.019.8029654
ACCGTTC4650.019.2038088
GAGTACA73650.018.2512471
CCGATCG1300.00804329318.0764589
GCAGAGT75900.017.8335349
ACCGCGT2403.4232198E-617.6245488
Produced by FastQC (version 0.11.5)