##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032984_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3459134 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89762986921004 34.0 31.0 34.0 30.0 34.0 2 31.944478010970375 34.0 31.0 34.0 30.0 34.0 3 32.08967562401457 34.0 31.0 34.0 30.0 34.0 4 35.39986713437525 37.0 35.0 37.0 35.0 37.0 5 35.4766273870859 37.0 37.0 37.0 35.0 37.0 6 35.539020460034216 37.0 37.0 37.0 35.0 37.0 7 35.46828888386515 37.0 37.0 37.0 35.0 37.0 8 35.48290843893298 37.0 37.0 37.0 35.0 37.0 9 37.16892493901653 39.0 39.0 39.0 35.0 39.0 10-11 37.158430983014824 39.0 38.0 39.0 35.0 39.0 12-13 37.1131567901099 39.0 38.0 39.0 35.0 39.0 14-15 38.62343103794187 41.0 39.5 41.0 36.0 41.0 16-17 38.64817263511618 41.0 39.5 41.0 35.5 41.0 18-19 38.66610313448395 41.0 39.5 41.0 35.0 41.0 20-21 38.7069854767118 41.0 40.0 41.0 35.0 41.0 22-23 38.693978608518776 41.0 39.0 41.0 35.0 41.0 24-25 38.72462457366497 41.0 39.0 41.0 35.0 41.0 26-27 38.695278789431114 41.0 39.0 41.0 35.0 41.0 28-29 38.673863169220965 41.0 39.0 41.0 35.0 41.0 30-31 38.627899497388654 41.0 39.0 41.0 35.0 41.0 32-33 38.575892549985056 41.0 39.0 41.0 35.0 41.0 34-35 38.50828820161347 41.0 39.0 41.0 34.0 41.0 36-37 38.42733961737244 41.0 39.0 41.0 34.0 41.0 38-39 38.34523583070214 41.0 39.0 41.0 34.0 41.0 40-41 38.27033818290937 41.0 39.0 41.0 34.0 41.0 42-43 38.17197425714066 41.0 38.5 41.0 33.0 41.0 44-45 38.06421592803285 40.0 38.0 41.0 33.0 41.0 46-47 37.95686535994269 40.0 38.0 41.0 33.0 41.0 48-49 37.835576910290264 40.0 38.0 41.0 33.0 41.0 50-51 37.71405689979053 40.0 38.0 41.0 33.0 41.0 52-53 37.5685772797469 40.0 37.5 41.0 33.0 41.0 54-55 37.4160690218997 40.0 37.0 41.0 32.0 41.0 56-57 37.24112841537795 40.0 37.0 41.0 32.0 41.0 58-59 37.046084944960214 40.0 36.0 41.0 31.5 41.0 60-61 36.813508959178805 39.5 36.0 41.0 31.0 41.0 62-63 36.56864839581236 39.0 35.0 41.0 31.0 41.0 64-65 36.301303158536214 39.0 35.0 41.0 31.0 41.0 66-67 36.01146269557641 38.5 35.0 40.5 30.5 41.0 68-69 35.70298938982994 37.5 35.0 40.0 30.0 41.0 70-71 35.363767492094844 37.0 35.0 40.0 30.0 41.0 72-73 35.0648510580972 37.0 35.0 39.0 30.0 41.0 74-75 34.77841202451249 36.0 35.0 39.0 30.0 41.0 76-77 34.44272988557252 36.0 34.0 39.0 30.0 40.0 78-79 34.061593595391216 35.0 34.0 37.5 29.0 39.5 80-81 33.72943242441605 35.0 34.0 37.0 29.0 39.0 82-83 33.39886804038236 35.0 34.0 36.5 29.0 39.0 84-85 33.212901986450944 35.0 34.0 36.0 29.0 37.5 86-87 32.971325482042616 35.0 34.0 36.0 29.0 37.0 88-89 32.72872849100382 35.0 34.0 35.5 29.0 37.0 90-91 32.525744882967814 35.0 34.0 35.0 28.5 36.0 92-93 32.334567698157976 35.0 34.0 35.0 28.0 36.0 94-95 32.154986334730026 35.0 34.0 35.0 27.0 36.0 96-97 31.98602135679046 35.0 34.0 35.0 27.0 36.0 98-99 31.814386490954092 35.0 34.0 35.0 27.0 35.5 100 31.691996609556032 35.0 33.0 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2696964094709138 1101 2 0.30492493643918905 1101 3 0.3104682346427836 1101 4 0.2828979691330744 1101 5 0.25831558496185636 1101 6 0.2907740520894677 1101 7 0.2573541003375013 1101 8 0.23509653431179345 1101 9 0.3129253962684544 1101 10-11 0.3297931491000341 1101 12-13 0.12210648376483846 1101 14-15 0.1872215185678172 1101 16-17 0.20932118857937354 1101 18-19 0.22164267209122812 1101 20-21 0.1729078929575465 1101 22-23 0.21009097827592882 1101 24-25 0.21577039195250336 1101 26-27 0.22420572248437054 1101 28-29 0.26415893711772753 1101 30-31 0.23534376622438202 1101 32-33 0.2216187247619743 1101 34-35 0.25216250824890807 1101 36-37 0.2378750184544529 1101 38-39 0.22709903902891426 1101 40-41 0.2430581469070603 1101 42-43 0.2535103958615039 1101 44-45 0.2538861739641902 1101 46-47 0.1867334343570093 1101 48-49 0.19915399148805335 1101 50-51 0.19579179061874896 1101 52-53 0.2722432836635562 1101 54-55 0.24587196968359137 1101 56-57 0.2640146626754216 1101 58-59 0.26826941665250104 1101 60-61 0.30411523712384536 1101 62-63 0.297202058975941 1101 64-65 0.2664968889504493 1101 66-67 0.2911909685715486 1101 68-69 0.32491856840553623 1101 70-71 0.2429595540326588 1101 72-73 0.22366815589781908 1101 74-75 0.22772393100889587 1101 76-77 0.24783628064093222 1101 78-79 0.28908630588038875 1101 80-81 0.29237079114329134 1101 82-83 0.22018228240078486 1101 84-85 0.13842746392371197 1101 86-87 0.15579469103649046 1101 88-89 0.12419436702590758 1101 90-91 0.13801186672523613 1101 92-93 0.15593883599894554 1101 94-95 0.18820426710132665 1101 96-97 0.10009406673366072 1101 98-99 0.15061342162342228 1101 100 0.10022980155312666 1102 1 0.23828960358200746 1102 2 0.2278161966298491 1102 3 0.30366685221385836 1102 4 0.29267261201112404 1102 5 0.216288906160095 1102 6 0.17363911284409994 1102 7 0.22243321225172963 1102 8 0.24699721945134456 1102 9 0.2971651763345733 1102 10-11 0.30941420717287116 1102 12-13 0.35154689606355305 1102 14-15 0.38881637057745166 1102 16-17 0.3653174464339557 1102 18-19 0.3291654226343752 1102 20-21 0.3634861030235683 1102 22-23 0.3263599444254126 1102 24-25 0.36110548958737354 1102 26-27 0.38820537787121623 1102 28-29 0.37953986167505604 1102 30-31 0.426928555971692 1102 32-33 0.39990336860096676 1102 34-35 0.37017751438457225 1102 36-37 0.42155639266567846 1102 38-39 0.3771366703436456 1102 40-41 0.4270672791556791 1102 42-43 0.4050541854795924 1102 44-45 0.3613700671650406 1102 46-47 0.37994243450390286 1102 48-49 0.4055229285884181 1102 50-51 0.41081727763334897 1102 52-53 0.4111381578742268 1102 54-55 0.3674985658665548 1102 56-57 0.40495342425502656 1102 58-59 0.3664801895690033 1102 60-61 0.43278526229607195 1102 62-63 0.42589720114543184 1102 64-65 0.4366913855225363 1102 66-67 0.421647437413327 1102 68-69 0.3514236939739561 1102 70-71 0.3804703646136005 1102 72-73 0.3584205811681258 1102 74-75 0.36344880600378815 1102 76-77 0.3920330282438371 1102 78-79 0.35329584393308266 1102 80-81 0.31519865134963965 1102 82-83 0.33110247684213334 1102 84-85 0.34053479545813303 1102 86-87 0.29284371998951997 1102 88-89 0.31995975615712524 1102 90-91 0.3314193728857475 1102 92-93 0.3212209162253217 1102 94-95 0.3513152402690025 1102 96-97 0.2913781815030916 1102 98-99 0.353488224326874 1102 100 0.3922908643533525 1103 1 0.07362440238959778 1103 2 0.11910544020394198 1103 3 0.1628111788608777 1103 4 0.06782795721917267 1103 5 0.06596446369970721 1103 6 0.018359406841653936 1103 7 0.05662929947777684 1103 8 0.07365553615881026 1103 9 0.12978498308661557 1103 10-11 0.09382342992849146 1103 12-13 0.06449668840767941 1103 14-15 0.051668015639307896 1103 16-17 0.07034908671432305 1103 18-19 0.08562162660469852 1103 20-21 0.0461599235742014 1103 22-23 0.11540267520058478 1103 24-25 0.13927490207888127 1103 26-27 0.16888654836060368 1103 28-29 0.18373787418730103 1103 30-31 0.16359135796130175 1103 32-33 0.17280691176228657 1103 34-35 0.14123532163168306 1103 36-37 0.15299170410716556 1103 38-39 0.12190659864260311 1103 40-41 0.14888967890856009 1103 42-43 0.1205588537697011 1103 44-45 0.12681591901954903 1103 46-47 0.11350573727949609 1103 48-49 0.14102203258983792 1103 50-51 0.10293189774898792 1103 52-53 0.008568387406427291 1103 54-55 0.08608714066815537 1103 56-57 0.1013144006078619 1103 58-59 0.06139616457836894 1103 60-61 0.09522309776661331 1103 62-63 0.10463943917427798 1103 64-65 0.09248893004213699 1103 66-67 0.04658329999443822 1103 68-69 0.08267936140298104 1103 70-71 0.12258504820021443 1103 72-73 0.10099069014778905 1103 74-75 0.04664577547636384 1103 76-77 0.05577206098850951 1103 78-79 0.08923985830290349 1103 80-81 0.13008767235218244 1103 82-83 0.15829945054611017 1103 84-85 0.1205116073957484 1103 86-87 0.1458852913615658 1103 88-89 0.17285126099044135 1103 90-91 0.12956580888479152 1103 92-93 0.15056138933795893 1103 94-95 0.07128350478217271 1103 96-97 0.09811152090143338 1103 98-99 0.1380904623134036 1103 100 0.1941293645765576 1104 1 0.052050849167549984 1104 2 0.0935094918119681 1104 3 0.13312989967087674 1104 4 0.08162422699320615 1104 5 0.14752907835610785 1104 6 0.08070378946101897 1104 7 0.0938718738659361 1104 8 0.1667940900207796 1104 9 0.15379807402229773 1104 10-11 0.12948174513075372 1104 12-13 0.1322805335754751 1104 14-15 0.1971854062282219 1104 16-17 0.2168978862040163 1104 18-19 0.22938350321592083 1104 20-21 0.14464816834462368 1104 22-23 0.24524840368353296 1104 24-25 0.2590179995762867 1104 26-27 0.2781236620977552 1104 28-29 0.28057406887738523 1104 30-31 0.2298955861060037 1104 32-33 0.22278842518334585 1104 34-35 0.2577525923625572 1104 36-37 0.271675744095063 1104 38-39 0.2992251914685937 1104 40-41 0.3092078715305888 1104 42-43 0.29275505934858614 1104 44-45 0.19838705476588814 1104 46-47 0.221510243129174 1104 48-49 0.21385639081098162 1104 50-51 0.22239359294795946 1104 52-53 0.19717687619969837 1104 54-55 0.21195400885884652 1104 56-57 0.21224494592131293 1104 58-59 0.24240110313589014 1104 60-61 0.31500731266598336 1104 62-63 0.2651676880889511 1104 64-65 0.210092256813752 1104 66-67 0.25601033793661543 1104 68-69 0.260295913188763 1104 70-71 0.20392717383175807 1104 72-73 0.28048744890200084 1104 74-75 0.24279250203142766 1104 76-77 0.22357737902876096 1104 78-79 0.21966606536477684 1104 80-81 0.2151898868818236 1104 82-83 0.17393803995918233 1104 84-85 0.13565040909976034 1104 86-87 0.08172676589985883 1104 88-89 0.11813468462359822 1104 90-91 0.17506075148498468 1104 92-93 0.09707841628976865 1104 94-95 0.1072871939719846 1104 96-97 0.1621775525236373 1104 98-99 0.16850588215173445 1104 100 0.13461757278012954 1105 1 -0.10654503858120634 1105 2 -0.06738194941937437 1105 3 -0.05841783042862403 1105 4 -0.10464462406400798 1105 5 -0.08024707991503277 1105 6 -0.04557963715589608 1105 7 0.009634272808725086 1105 8 -0.027676719991660548 1105 9 -0.025441081761286455 1105 10-11 0.008669692114587235 1105 12-13 0.020526413550598477 1105 14-15 0.0495571413028415 1105 16-17 0.006315058156751263 1105 18-19 0.0051329784957729885 1105 20-21 0.07934315046527018 1105 22-23 0.12192857703551852 1105 24-25 0.0898291780588778 1105 26-27 0.08474601906293344 1105 28-29 0.1193133801336046 1105 30-31 0.09245175059604804 1105 32-33 0.1471169777330985 1105 34-35 0.11253259858145981 1105 36-37 0.12432072232889624 1105 38-39 0.08705216985789122 1105 40-41 0.1563184125753594 1105 42-43 0.18109723518814036 1105 44-45 0.10653906813654146 1105 46-47 0.04740003246375579 1105 48-49 0.02029179193038999 1105 50-51 0.047433918663138286 1105 52-53 0.0464547058016862 1105 54-55 0.035105633547900084 1105 56-57 0.07869978112030651 1105 58-59 0.1329775179368795 1105 60-61 0.11308693409011283 1105 62-63 0.07762677935139095 1105 64-65 0.12088827671425406 1105 66-67 0.12786687556099707 1105 68-69 0.04468976424598026 1105 70-71 0.09535003996982283 1105 72-73 0.09066313298159656 1105 74-75 0.07432244994809167 1105 76-77 0.00899890421533911 1105 78-79 0.0751250011545821 1105 80-81 0.04841594409575123 1105 82-83 0.060724906408196944 1105 84-85 0.025119275704248878 1105 86-87 0.049956029736932805 1105 88-89 0.04703452137732711 1105 90-91 0.04531135394145025 1105 92-93 0.006164042844496009 1105 94-95 0.026516827056568104 1105 96-97 0.023630347144283803 1105 98-99 0.06322875217661306 1105 100 0.05836660107614833 1106 1 0.034690830812159845 1106 2 0.03791170723805237 1106 3 0.06750054498488112 1106 4 0.03433179249320517 1106 5 0.008930768097712871 1106 6 0.012308114595413144 1106 7 0.06582053685571765 1106 8 0.020417624421163794 1106 9 0.04442944722052289 1106 10-11 0.0739516315422506 1106 12-13 0.09265856170685538 1106 14-15 0.08649693382083967 1106 16-17 0.09687046928974752 1106 18-19 0.11914897823429271 1106 20-21 0.1496425194147335 1106 22-23 0.18335367417869008 1106 24-25 0.15502965176484906 1106 26-27 0.20816603633282682 1106 28-29 0.1914350006458676 1106 30-31 0.2153295371912023 1106 32-33 0.2105242934195104 1106 34-35 0.2027746507803343 1106 36-37 0.2151960294168518 1106 38-39 0.24742697298714944 1106 40-41 0.1940389990221405 1106 42-43 0.19961910456326137 1106 44-45 0.13422787402046765 1106 46-47 0.23166141372105642 1106 48-49 0.19967836093700697 1106 50-51 0.16198257158077922 1106 52-53 0.1759009441262691 1106 54-55 0.18335770259100315 1106 56-57 0.18978892480098608 1106 58-59 0.1657716990762026 1106 60-61 0.24983922432775074 1106 62-63 0.2208883840835938 1106 64-65 0.20377405215694466 1106 66-67 0.2313336629345173 1106 68-69 0.2243581472606735 1106 70-71 0.2331550566012197 1106 72-73 0.24595143080218662 1106 74-75 0.21076681610031756 1106 76-77 0.1490550693860868 1106 78-79 0.22296236843785522 1106 80-81 0.21043038027010397 1106 82-83 0.2591527719036648 1106 84-85 0.22866397524452253 1106 86-87 0.19114505681539384 1106 88-89 0.21773783640700373 1106 90-91 0.17930665564776405 1106 92-93 0.11885737903588023 1106 94-95 0.24963918184349154 1106 96-97 0.24487184521662186 1106 98-99 0.17949582444261836 1106 100 0.22987229740568793 1107 1 0.17353723419411793 1107 2 0.14503285600958904 1107 3 0.1803382480810498 1107 4 0.19166058906557737 1107 5 0.14835337259624026 1107 6 0.15933353118651894 1107 7 0.1333049658762846 1107 8 0.13196406798287796 1107 9 0.1493281477655941 1107 10-11 0.12186330659611855 1107 12-13 0.12525157388230213 1107 14-15 0.2017302819249025 1107 16-17 0.17626941794579665 1107 18-19 0.18831552979604282 1107 20-21 0.22590701225700371 1107 22-23 0.282794445322736 1107 24-25 0.2653463652652306 1107 26-27 0.29508785447920616 1107 28-29 0.283085866214698 1107 30-31 0.30003795825203383 1107 32-33 0.24621676366383838 1107 34-35 0.3385144100360762 1107 36-37 0.3309018836472575 1107 38-39 0.27546539619575583 1107 40-41 0.22311991252838226 1107 42-43 0.267190680528536 1107 44-45 0.25468442527100876 1107 46-47 0.31273665196079037 1107 48-49 0.3090620389469265 1107 50-51 0.3811243705577496 1107 52-53 0.30611175452372663 1107 54-55 0.28832617920426173 1107 56-57 0.3448231748667894 1107 58-59 0.29967440585738103 1107 60-61 0.33732034812765477 1107 62-63 0.3687968080407842 1107 64-65 0.36023238496457566 1107 66-67 0.30814442413034726 1107 68-69 0.3394006900000619 1107 70-71 0.3886039403468615 1107 72-73 0.398829772241136 1107 74-75 0.4373449044620443 1107 76-77 0.39720230083955954 1107 78-79 0.3776945846738897 1107 80-81 0.43715895527785875 1107 82-83 0.4087489319379287 1107 84-85 0.3576275932784725 1107 86-87 0.31939145878574493 1107 88-89 0.34089115223854094 1107 90-91 0.364033328928528 1107 92-93 0.3921504719109308 1107 94-95 0.34976712657733344 1107 96-97 0.33697144623867814 1107 98-99 0.3426162175261709 1107 100 0.30272037768063953 1108 1 0.21267236042865179 1108 2 0.2677931127552249 1108 3 0.2542829296122804 1108 4 0.23028275658400332 1108 5 0.2305074553443518 1108 6 0.23497046278831846 1108 7 0.22891195793795305 1108 8 0.2938032243417368 1108 9 0.2856673257554547 1108 10-11 0.29311649070374557 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-0.21268136307062235 2214 68-69 -0.2373483691576439 2214 70-71 -0.19323977246232715 2214 72-73 -0.27880378492385915 2214 74-75 -0.25976414848913976 2214 76-77 -0.19805880284788913 2214 78-79 -0.21841616420891796 2214 80-81 -0.17190856108214092 2214 82-83 -0.19264481327090266 2214 84-85 -0.23518106905314795 2214 86-87 -0.26479570775158834 2214 88-89 -0.23100791040194224 2214 90-91 -0.21752735192647066 2214 92-93 -0.21188185999707088 2214 94-95 -0.18265082264278476 2214 96-97 -0.15352655596348797 2214 98-99 -0.2117666153906157 2214 100 -0.21634387187566162 2215 1 -0.1984783349983772 2215 2 -0.23360816918733818 2215 3 -0.3167468582973463 2215 4 -0.2200342835315965 2215 5 -0.20589580899303428 2215 6 -0.22437692906128603 2215 7 -0.24800925901931237 2215 8 -0.22235833088337387 2215 9 -0.4149962910324376 2215 10-11 -0.3071795384008382 2215 12-13 -0.2953875428047752 2215 14-15 -0.3661976620610474 2215 16-17 -0.3894491639438371 2215 18-19 -0.3470392935874216 2215 20-21 -0.40843584683890555 2215 22-23 -0.36175576356339434 2215 24-25 -0.3405925741714384 2215 26-27 -0.35108930075210054 2215 28-29 -0.30389702792213313 2215 30-31 -0.30832633424802935 2215 32-33 -0.3895558934917389 2215 34-35 -0.3788639321518801 2215 36-37 -0.40696949753115064 2215 38-39 -0.38779265757396786 2215 40-41 -0.38410695880293133 2215 42-43 -0.41160581755679004 2215 44-45 -0.4062242152875086 2215 46-47 -0.4044300830676377 2215 48-49 -0.33373690804096867 2215 50-51 -0.309199464843779 2215 52-53 -0.3652307940755435 2215 54-55 -0.3719784649645419 2215 56-57 -0.3783665733362227 2215 58-59 -0.353711882435519 2215 60-61 -0.3596448480397356 2215 62-63 -0.30658674973990685 2215 64-65 -0.30013807784459345 2215 66-67 -0.27579169299563944 2215 68-69 -0.23299073916088275 2215 70-71 -0.3095659107534985 2215 72-73 -0.36044204994522744 2215 74-75 -0.2609418789365492 2215 76-77 -0.2645414473663479 2215 78-79 -0.23448177458101327 2215 80-81 -0.30254447291194 2215 82-83 -0.22721202897914594 2215 84-85 -0.21541425448686624 2215 86-87 -0.2526937652632739 2215 88-89 -0.23764340642386372 2215 90-91 -0.27050194086437074 2215 92-93 -0.2367638798412912 2215 94-95 -0.21233785967612562 2215 96-97 -0.23674047804703235 2215 98-99 -0.27243628864430747 2215 100 -0.274035739476723 2216 1 -0.03404970564333709 2216 2 -0.1059458713467123 2216 3 -0.14324109271128904 2216 4 -0.11401759897029251 2216 5 -0.07172208963548599 2216 6 -0.1443829002361383 2216 7 -0.12243034450464307 2216 8 -0.17168353303001993 2216 9 -0.1915778696760242 2216 10-11 -0.24123177928735373 2216 12-13 -0.2518624617684324 2216 14-15 -0.3436827843232564 2216 16-17 -0.2684703753753084 2216 18-19 -0.29036846274784267 2216 20-21 -0.3204187764020432 2216 22-23 -0.28550275359437194 2216 24-25 -0.34168047011068836 2216 26-27 -0.3207242263908938 2216 28-29 -0.32402664743258924 2216 30-31 -0.3159881234368882 2216 32-33 -0.35371103635407763 2216 34-35 -0.3519858902126103 2216 36-37 -0.34301289597146933 2216 38-39 -0.3466242818539058 2216 40-41 -0.28096561896873595 2216 42-43 -0.29807525232482845 2216 44-45 -0.2569462801546578 2216 46-47 -0.21318711074589203 2216 48-49 -0.1787549185696946 2216 50-51 -0.22303907780260346 2216 52-53 -0.25197594003496704 2216 54-55 -0.22861792807476888 2216 56-57 -0.24892916438534485 2216 58-59 -0.2053735822186482 2216 60-61 -0.2255530534726944 2216 62-63 -0.2081077595254186 2216 64-65 -0.21545723293640862 2216 66-67 -0.24924373941946243 2216 68-69 -0.24267767000389995 2216 70-71 -0.257755067378838 2216 72-73 -0.30813277745068746 2216 74-75 -0.3105408313019069 2216 76-77 -0.36091085137766044 2216 78-79 -0.31678314495623994 2216 80-81 -0.2838168183065477 2216 82-83 -0.30323224198569676 2216 84-85 -0.35203626520139863 2216 86-87 -0.2871337330093269 2216 88-89 -0.26634340348110186 2216 90-91 -0.32728200910987226 2216 92-93 -0.314683050741003 2216 94-95 -0.26520247127650265 2216 96-97 -0.2593736799882329 2216 98-99 -0.22776905199869546 2216 100 -0.2853667931553865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36315.0 3 5540.0 4 3497.0 5 3071.0 6 2642.0 7 3110.0 8 3494.0 9 3929.0 10 4149.0 11 4383.0 12 4777.0 13 5405.0 14 5799.0 15 6154.0 16 6028.0 17 6047.0 18 5887.0 19 5772.0 20 6083.0 21 6396.0 22 7373.0 23 10134.0 24 15468.0 25 21443.0 26 23666.0 27 20721.0 28 21592.0 29 27021.0 30 33425.0 31 40465.0 32 52355.0 33 70649.0 34 102482.0 35 160862.0 36 304438.0 37 667953.0 38 1180002.0 39 569460.0 40 1147.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27893714205038 19.64098282304718 13.241864873403438 23.838215161499 2 15.33458144445862 19.91483681250819 40.87792495305118 23.872656789982013 3 18.28932929096192 25.321961921509367 29.706945431747084 26.68176335578163 4 12.159662623147845 16.4574504681723 39.2417874191371 32.14109948954275 5 12.877881341613296 38.266930449696446 35.78149483736723 13.073693371323028 6 31.11219638649588 38.1546467274318 17.816158464607934 12.916998421464381 7 27.66724328033869 32.39264006027103 22.39668185304481 17.54343480634547 8 25.13270321667332 36.79710403637082 20.242842474403258 17.827350272552604 9 24.99666561065616 17.04363348623787 21.025140283039747 36.93456062006623 10-11 23.873258945136115 26.981853320670897 29.03947512127803 20.10541261291496 12-13 24.466634842106785 24.31107060712581 29.030363523268093 22.19193102749931 14-15 21.769977687047277 26.396635336918354 25.86679492696757 25.9665920490668 16-17 21.580621988875055 29.830507640984944 26.47022745466081 22.11864291547919 18-19 21.52691830340482 28.769386993464618 28.325478726020304 21.37821597711026 20-21 22.64726682340103 28.13050081272259 27.796385543388624 21.425846820487752 22-23 22.303821693366228 27.887215962308886 27.840203326035525 21.96875901828936 24-25 22.064937326811904 28.020061900730852 27.78998825980717 22.125012512650073 26-27 21.903976781398963 27.910577603782283 27.913586393788698 22.271859221030056 28-29 21.890875995502032 28.06053682254584 27.963622824606205 22.08496435734592 30-31 22.117879086044198 28.445495735414905 27.59230387118034 21.844321307360556 32-33 21.765871787468516 28.132408054060964 28.04866829334057 22.053051865129948 34-35 21.934697724635505 27.795574479558578 28.161233778651074 22.108494017154847 36-37 22.194477644561054 28.22239737162114 27.627586927130444 21.955538056687367 38-39 21.99454941077311 28.375117760759892 27.78958660024668 21.84074622822032 40-41 21.861519452201744 28.01866631410452 28.061511872501338 22.058302361192396 42-43 21.760426566679254 27.990226924562446 28.02655047758746 22.222796031170844 44-45 21.919952343527022 28.118751181683503 27.969622180975108 21.991674293814363 46-47 21.917524639752568 28.25754671535064 27.845647302538406 21.979281342358387 48-49 21.784409538057226 28.102756888967395 27.945430764714914 22.167402808260466 50-51 21.870344814815404 28.02375006130359 27.930959204863086 22.174945919017915 52-53 21.930861358439447 27.967624155962824 27.940194846472544 22.16131963912519 54-55 21.975628093849476 28.35791486571118 27.76426322746558 21.902193812973767 56-57 21.806090861508807 28.100411228776352 27.882124061874407 22.211373847840434 58-59 21.703513303241103 27.73109280167626 28.225846817791357 22.339547077291282 60-61 21.97233978809644 28.125487781643947 27.80983564534298 22.09233678491663 62-63 21.783003511833705 28.099255449591677 27.854862145048166 22.262878893526455 64-65 21.759265420765168 27.85736728903877 28.019026451634183 22.36434083856188 66-67 21.653335941083743 27.968455864089524 27.89640036413994 22.48180783068679 68-69 22.04159938154832 28.056979374480218 27.793625312773635 22.10779593119783 70-71 21.90911031954339 27.835163544371767 27.953580455220877 22.302145680863966 72-73 21.740782680848554 27.93446008422568 28.016582437128733 22.30817479779703 74-75 21.774017976549622 28.00812105492706 27.8949701970014 22.322890771521916 76-77 21.801424207535565 27.930475457684384 27.831038130612967 22.43706220416708 78-79 21.876645477259288 27.979348139301546 27.92594064882868 22.218065734610484 80-81 21.76682445587604 27.79324584260899 28.049784103683468 22.390145597831506 82-83 21.654584562266077 27.855088812255335 28.157742959804803 22.33258366567378 84-85 21.639520791424957 27.811245008967294 27.985687044709756 22.56354715489799 86-87 21.8324993287845 27.861692574042014 27.9385496421727 22.367258455000787 88-89 21.58941175228261 27.75671366925873 27.98586088893383 22.66801368952483 90-91 21.577177451014713 27.67180977602551 28.149374573099166 22.601638199860613 92-93 21.801605442546858 27.795529559682198 27.95862254915717 22.444242448613775 94-95 21.513970905615093 27.617577558603628 28.12978430902861 22.73866722675267 96-97 21.61203528343232 27.512916096326588 28.138466396452156 22.736582223788933 98-99 21.600821950582 27.65327641125938 28.03344157379872 22.7124600643599 100 21.645381322320077 27.47012886924513 28.06821686229743 22.816272946137364 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2872.0 1 1668.0 2 423.5 3 336.0 4 277.0 5 254.5 6 228.0 7 214.5 8 225.5 9 229.5 10 223.5 11 228.0 12 262.5 13 279.0 14 307.0 15 393.5 16 509.0 17 672.0 18 889.5 19 1276.5 20 1873.5 21 2721.5 22 4066.5 23 6218.0 24 9189.5 25 13019.0 26 17775.0 27 23980.0 28 31952.5 29 41384.0 30 51056.5 31 60940.5 32 71782.5 33 83445.5 34 94153.5 35 103372.5 36 111583.0 37 118917.0 38 124670.0 39 126662.5 40 128583.0 41 131224.0 42 131353.0 43 130177.5 44 127774.5 45 124972.0 46 122877.0 47 118756.0 48 115022.5 49 111667.0 50 107845.5 51 103761.5 52 96185.5 53 90729.5 54 87281.0 55 81502.5 56 75196.5 57 69261.0 58 62922.0 59 56863.5 60 50764.5 61 43564.5 62 37379.5 63 31282.0 64 25383.0 65 20689.0 66 16254.0 67 12269.0 68 9322.0 69 7416.0 70 5712.5 71 4236.5 72 3268.0 73 2464.0 74 1894.0 75 1376.0 76 872.0 77 611.0 78 398.5 79 237.5 80 165.5 81 111.0 82 87.5 83 74.5 84 55.0 85 39.5 86 32.5 87 33.0 88 26.5 89 13.5 90 7.5 91 7.0 92 7.5 93 7.0 94 7.0 95 6.0 96 2.0 97 0.0 98 1.0 99 2.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0763774979517995 2 0.0783722168612144 3 0.07941293977047434 4 0.08155220352839757 5 0.07973093843719266 6 0.07996221019480598 7 0.07941293977047434 8 0.0791238500734577 9 0.07889257831584437 10-11 0.07853121619457355 12-13 0.07759167467926945 14-15 0.07578486407291536 16-17 0.07449841492119125 18-19 0.07412259831506962 20-21 0.0735010554664838 22-23 0.07564031922440703 24-25 0.07608840825478284 26-27 0.07501877637582123 28-29 0.07114497443579809 30-31 0.07640640692150116 32-33 0.07493204946671624 34-35 0.0723013332238647 36-37 0.06945379970825068 38-39 0.07063906746601895 40-41 0.07322642025431798 42-43 0.07802530922479442 44-45 0.07893594177039687 46-47 0.08178347528601089 48-49 0.08492009849864157 50-51 0.08598973037760317 52-53 0.08606200280185734 54-55 0.0862210021352165 56-57 0.08403837492274077 58-59 0.08299765201348083 60-61 0.08087284274040843 62-63 0.0815232945586959 64-65 0.08059820752824261 66-67 0.08009230055846348 68-69 0.07980321086144683 70-71 0.0781120361338994 72-73 0.07822767201270607 74-75 0.07766394710352359 76-77 0.07763503813382193 78-79 0.07776512849747942 80-81 0.0789070328006952 82-83 0.07844448928546856 84-85 0.0776205836489711 86-87 0.07857457964912605 88-89 0.07581377304261702 90-91 0.07328423819372132 92-93 0.07276387673909135 94-95 0.07290842158759968 96-97 0.07220015182990888 98-99 0.07165088140557724 100 0.07088479370848311 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3459134.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.8435766871715 #Duplication Level Percentage of deduplicated Percentage of total 1 74.35427018541232 45.23979740044803 2 14.835175520434282 18.05250278890385 3 5.025991144187762 9.173978328312987 4 2.1241168626234663 5.1695546889418 5 1.1399179063196427 3.467834127511958 6 0.6550291354330386 2.3912589260431036 7 0.40407536434106645 1.7209773292377706 8 0.2869498303872964 1.3967243208432258 9 0.20680550968600336 1.132450819911894 >10 0.9177427772047873 9.601298342671113 >50 0.039461658862527754 1.624939716529138 >100 0.010321442353858429 0.9230751793099635 >500 9.510850314209104E-5 0.038944770221302875 >1k 4.755425084465741E-5 0.06666326111388449 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.8908969701665213E-5 0.0 4 0.0 0.0 0.0 2.8908969701665213E-5 0.0 5 0.0 2.8908969701665213E-5 0.0 2.8908969701665213E-5 0.0 6 0.0 2.8908969701665213E-5 0.0 2.8908969701665213E-5 0.0 7 0.0 2.8908969701665213E-5 0.0 5.7817939403330426E-5 0.0 8 0.0 2.8908969701665213E-5 0.0 5.7817939403330426E-5 0.0 9 0.0 2.8908969701665213E-5 0.0 5.7817939403330426E-5 0.0 10-11 0.0 4.336345455249782E-5 0.0 8.672690910499564E-5 0.0 12-13 0.0 5.7817939403330426E-5 0.0 8.672690910499564E-5 0.0 14-15 0.0 5.7817939403330426E-5 0.0 1.0118139395582824E-4 0.0 16-17 0.0 5.7817939403330426E-5 0.0 1.1563587880666085E-4 0.0 18-19 0.0 5.7817939403330426E-5 0.0 1.1563587880666085E-4 0.0 20-21 0.0 5.7817939403330426E-5 0.0 1.3009036365749346E-4 0.0 22-23 0.0 5.7817939403330426E-5 0.0 1.589993333591587E-4 0.0 24-25 0.0 5.7817939403330426E-5 0.0 2.168172727624891E-4 0.0 26-27 0.0 5.7817939403330426E-5 0.0 3.7581660612164784E-4 0.0 28-29 0.0 5.7817939403330426E-5 0.0 0.0012575401820224368 0.0 30-31 0.0 5.7817939403330426E-5 0.0 0.005073524182642245 0.0 32-33 0.0 5.7817939403330426E-5 0.0 0.013659488184036814 0.0 34-35 0.0 5.7817939403330426E-5 0.0 0.02610479964060369 0.0 36-37 0.0 5.7817939403330426E-5 0.0 0.04933315679589169 0.0 38-39 0.0 5.7817939403330426E-5 0.0 0.09135234425726207 0.0 40-41 0.0 5.7817939403330426E-5 0.0 0.14096013626531959 0.0 42-43 0.0 5.7817939403330426E-5 0.0 0.19308300863742195 0.0 44-45 0.0 8.672690910499564E-5 0.0 0.25123340119232157 0.0 46-47 0.0 1.1563587880666085E-4 0.0 0.3230288274464071 0.0 48-49 0.0 1.1563587880666085E-4 0.0 0.4070527478842971 0.0 50-51 0.0 1.1563587880666085E-4 0.0 0.500139630323659 0.0 52-53 0.0 1.1563587880666085E-4 0.0 0.5951200502784801 0.0 54-55 0.0 1.1563587880666085E-4 0.0 0.6987587066589499 0.0 56-57 0.0 1.4454484850832608E-4 0.0 0.8214194651031155 0.0 58-59 0.0 2.023627879116565E-4 0.0 0.965747496338679 0.0 60-61 0.0 2.023627879116565E-4 0.0 1.1176785866057806 0.0 62-63 0.0 2.023627879116565E-4 0.0 1.274379656873657 0.0 64-65 0.0 2.023627879116565E-4 0.0 1.4396522366580768 0.0 66-67 0.0 2.7463521216581954E-4 0.0 1.625826001536801 0.0 68-69 0.0 2.8908969701665216E-4 0.0 1.8341151282372987 0.0 70-71 0.0 2.8908969701665216E-4 0.0 2.0660662466386097 0.0 72-73 0.0 2.8908969701665216E-4 0.0 2.306588874556464 0.0 74-75 0.0 2.8908969701665216E-4 0.0 2.55008912635359 0.0 76-77 0.0 3.4690763641998255E-4 0.0 2.812336845002246 0.0 78-79 0.0 3.4690763641998255E-4 0.0 3.1166471145668253 0.0 80-81 0.0 3.4690763641998255E-4 0.0 3.4456745532263278 0.0 82-83 0.0 3.4690763641998255E-4 0.0 3.7889396594639004 0.0 84-85 0.0 3.4690763641998255E-4 0.0 4.1231128947302995 0.0 86-87 0.0 3.4690763641998255E-4 0.0 4.474299058666128 0.0 88 0.0 3.4690763641998255E-4 0.0 4.760237677985299 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4245 0.0 36.205063 1 ATCAACG 4200 0.0 34.242474 3 TCAACGC 4260 0.0 33.760185 4 CAACGCA 4460 0.0 32.245808 5 AACGCAG 4680 0.0 30.52913 6 GGTATCA 2645 0.0 30.208038 1 TATCAAC 5295 0.0 28.22672 2 GTACATG 10340 0.0 26.363722 1 TACATGG 10200 0.0 25.850084 2 TATACCG 200 8.1490725E-10 25.849335 5 ACATGGG 10310 0.0 24.571014 3 ACGCAGA 5850 0.0 23.941265 7 CGCAGAG 6080 0.0 22.64909 8 ACACCGT 650 0.0 20.245634 6 CATGGGG 7215 0.0 19.802965 4 ACCGTTC 465 0.0 19.203808 8 GAGTACA 7365 0.0 18.251247 1 CCGATCG 130 0.008043293 18.076458 9 GCAGAGT 7590 0.0 17.833534 9 ACCGCGT 240 3.4232198E-6 17.624548 8 >>END_MODULE