FastQCFastQC Report
Wed 25 May 2016
SRR1032984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3459134
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57290.16561948742084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46110.1332992592943783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45350.048.8964161
GTATCAA86000.043.8115121
ATCAACG101550.036.225213
TCAACGC102300.035.959634
CAACGCA104650.035.146025
AACGCAG108450.034.044646
TATCAAC110200.033.6378822
ACGCAGA125900.029.1014147
CGCAGAG128300.028.5203788
GTACATG105350.027.024051
TACATGG104400.025.9090482
GCAGAGT148900.024.2899579
ACATGGG106950.024.0597233
GTGGTAT24850.021.5521581
CATGGGG71500.021.2510574
AGAGTAC134900.020.7348310-11
GAGTACT93100.020.55931512-13
CAGAGTA142100.019.70075610-11
AGTACTT97300.019.57531512-13
TGGTATC24150.018.699852
Produced by FastQC (version 0.11.5)