FastQCFastQC Report
Wed 25 May 2016
SRR1032983_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032983_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776695
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26500.034.585011
TCAACGC26150.033.0708434
GGTATCA16650.032.4625631
ATCAACG27250.031.9083543
CAACGCA27150.031.506545
AACGCAG29050.029.6076626
TATCAAC31950.027.508692
GTACATG54800.026.0730721
TACATGG56000.024.7590032
ACGCAGA34250.024.426357
CGCAGAG35450.023.3343438
ACATGGG56100.023.0392863
GCAGAGT41650.019.8608049
GTATTAG9650.019.4819221
CATGGGG37800.019.1482054
TATACCG1300.00804046118.0769795
GAGTACA42450.017.6042921
CATGGGA19650.017.2214264
ACCGTTC2505.0465987E-616.9200528
GTACCGG1750.002329232216.1143341
Produced by FastQC (version 0.11.5)