##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032983_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1776695 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.714033078271736 33.0 31.0 34.0 30.0 34.0 2 31.819653907958315 34.0 31.0 34.0 30.0 34.0 3 31.886763344299386 34.0 31.0 34.0 30.0 34.0 4 35.20085045548054 37.0 35.0 37.0 33.0 37.0 5 35.270045787262305 37.0 35.0 37.0 33.0 37.0 6 35.302031018267066 37.0 37.0 37.0 33.0 37.0 7 35.26679987279753 37.0 37.0 37.0 33.0 37.0 8 35.26312394642862 37.0 37.0 37.0 33.0 37.0 9 36.917508632601546 39.0 38.0 39.0 34.0 39.0 10-11 36.92041121295439 39.0 38.0 39.0 34.0 39.0 12-13 36.87347969122444 39.0 38.0 39.0 34.0 39.0 14-15 38.370535460503916 41.0 39.0 41.0 34.0 41.0 16-17 38.39591573117502 41.0 39.0 41.0 34.5 41.0 18-19 38.40705664168583 41.0 39.0 41.0 34.0 41.0 20-21 38.45143848550258 41.0 39.0 41.0 34.0 41.0 22-23 38.44288805900844 41.0 39.0 41.0 34.0 41.0 24-25 38.474375736972306 41.0 39.0 41.0 34.0 41.0 26-27 38.454129155538794 41.0 39.0 41.0 34.0 41.0 28-29 38.440669895508236 41.0 39.0 41.0 34.0 41.0 30-31 38.395330937499125 41.0 39.0 41.0 34.0 41.0 32-33 38.346854130844065 41.0 39.0 41.0 34.0 41.0 34-35 38.279302018635725 41.0 39.0 41.0 34.0 41.0 36-37 38.19354700722408 41.0 39.0 41.0 33.0 41.0 38-39 38.101682337148475 41.0 39.0 41.0 33.0 41.0 40-41 38.02597913541717 41.0 38.5 41.0 33.0 41.0 42-43 37.9147110224321 40.5 38.0 41.0 33.0 41.0 44-45 37.785339914841884 40.0 38.0 41.0 33.0 41.0 46-47 37.666629331427174 40.0 38.0 41.0 33.0 41.0 48-49 37.53880097596942 40.0 38.0 41.0 32.0 41.0 50-51 37.406873717773735 40.0 37.5 41.0 32.0 41.0 52-53 37.25722647950267 40.0 37.0 41.0 31.5 41.0 54-55 37.091214586634166 40.0 37.0 41.0 31.0 41.0 56-57 36.912442484500716 40.0 36.0 41.0 31.0 41.0 58-59 36.70567289264618 40.0 36.0 41.0 31.0 41.0 60-61 36.467599953846886 39.0 35.0 41.0 31.0 41.0 62-63 36.21903055955018 39.0 35.0 41.0 30.0 41.0 64-65 35.943779883435255 38.5 35.0 41.0 30.0 41.0 66-67 35.6468749560279 38.0 35.0 40.0 30.0 41.0 68-69 35.332238791689065 37.0 35.0 40.0 29.5 41.0 70-71 34.99307956627334 37.0 35.0 39.5 29.0 41.0 72-73 34.69645662311201 36.0 34.0 39.0 29.0 41.0 74-75 34.41371873056434 36.0 34.0 39.0 29.0 41.0 76-77 34.09083072784017 35.5 34.0 38.5 29.0 40.0 78-79 33.72091692721598 35.0 34.0 37.0 29.0 39.0 80-81 33.39996876222425 35.0 34.0 37.0 28.5 39.0 82-83 33.079715145255655 35.0 34.0 36.5 28.0 39.0 84-85 32.90638320026791 35.0 34.0 36.0 28.0 37.0 86-87 32.67071050461672 35.0 34.0 36.0 28.0 37.0 88-89 32.43965621561382 35.0 34.0 35.5 27.0 37.0 90-91 32.240092700210226 35.0 34.0 35.0 27.0 36.0 92-93 32.049188521383805 35.0 34.0 35.0 27.0 36.0 94-95 31.873167876309665 35.0 33.5 35.0 26.5 36.0 96-97 31.70248241819783 35.0 33.0 35.0 26.0 36.0 98-99 31.52917073555112 35.0 33.0 35.0 25.0 35.0 100 31.40478078679796 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3548836807496478 1101 2 0.4737737884460351 1101 3 0.3909807192758308 1101 4 0.35940994909051227 1101 5 0.32653180941319704 1101 6 0.3416019627037201 1101 7 0.407736510521282 1101 8 0.4387879635250158 1101 9 0.5504668987459524 1101 10-11 0.40373837139078006 1101 12-13 0.2796348026652211 1101 14-15 0.2657467395873212 1101 16-17 0.2730643176665808 1101 18-19 0.23335356804395246 1101 20-21 0.20005883669627167 1101 22-23 0.2861780152229798 1101 24-25 0.19242577436014585 1101 26-27 0.20853051507565112 1101 28-29 0.22710937980255608 1101 30-31 0.23369384955623218 1101 32-33 0.21092042224705665 1101 34-35 0.2582436036694986 1101 36-37 0.2972218996044589 1101 38-39 0.24489880331624647 1101 40-41 0.24879454408883106 1101 42-43 0.27763385042564437 1101 44-45 0.17076022815432879 1101 46-47 0.28068051777982106 1101 48-49 0.21182345640349354 1101 50-51 0.23521376521026838 1101 52-53 0.12232602287625127 1101 54-55 0.24142114682368288 1101 56-57 0.22522792818066506 1101 58-59 0.21189338184428408 1101 60-61 0.2775632264324912 1101 62-63 0.3276939710797322 1101 64-65 0.37138825231674133 1101 66-67 0.30755874802640193 1101 68-69 0.2827535210380461 1101 70-71 0.29408481332305314 1101 72-73 0.27826578543511005 1101 74-75 0.32885548644541984 1101 76-77 0.3072412641340776 1101 78-79 0.3511970902204382 1101 80-81 0.3550622864633368 1101 82-83 0.3747905043253894 1101 84-85 0.3356404683365213 1101 86-87 0.2609929589865274 1101 88-89 0.20481542081106596 1101 90-91 0.280943199511114 1101 92-93 0.24290708909006753 1101 94-95 0.11926329220181486 1101 96-97 0.08704972818075518 1101 98-99 0.1304431800402739 1101 100 0.1056446607956012 1102 1 0.3033833097157519 1102 2 0.29146461546630675 1102 3 0.3527386839492479 1102 4 0.24749486866231507 1102 5 0.3379561899337915 1102 6 0.25272908316028264 1102 7 0.3218435050947477 1102 8 0.27462888566331856 1102 9 0.35011811822224104 1102 10-11 0.30371810956294354 1102 12-13 0.3577943706207165 1102 14-15 0.3940933830943152 1102 16-17 0.32564835013399573 1102 18-19 0.31338250609039164 1102 20-21 0.3473694254062778 1102 22-23 0.35760953067781287 1102 24-25 0.37495254551665624 1102 26-27 0.3902371151496027 1102 28-29 0.3994542361124189 1102 30-31 0.40200651553653444 1102 32-33 0.3445692570620764 1102 34-35 0.3874898203792725 1102 36-37 0.4503444291118228 1102 38-39 0.39235823853078244 1102 40-41 0.4565232191644526 1102 42-43 0.44336226746601426 1102 44-45 0.3779496268889204 1102 46-47 0.3664548962655658 1102 48-49 0.41137890843707936 1102 50-51 0.4762373858257263 1102 52-53 0.4368525411928488 1102 54-55 0.4278015617755244 1102 56-57 0.37171522860383277 1102 58-59 0.38925052946382266 1102 60-61 0.4258875992466571 1102 62-63 0.44965467525617697 1102 64-65 0.45299293712842115 1102 66-67 0.441666071638096 1102 68-69 0.4671087922737911 1102 70-71 0.460395974386401 1102 72-73 0.4409240160233736 1102 74-75 0.42595986343317804 1102 76-77 0.42500906878234446 1102 78-79 0.3905462526016734 1102 80-81 0.41073460248551186 1102 82-83 0.4984754554155941 1102 84-85 0.49374169261849943 1102 86-87 0.4593951320242695 1102 88-89 0.48793047768705833 1102 90-91 0.4924197932034531 1102 92-93 0.5337580079293218 1102 94-95 0.5429039963089934 1102 96-97 0.6654863809614291 1102 98-99 0.5953917764757435 1102 100 0.6147049016342656 1103 1 0.1939371282538005 1103 2 0.28885889367198914 1103 3 0.3122614180213148 1103 4 0.22627601032277056 1103 5 0.28162285464508585 1103 6 0.23281953842833758 1103 7 0.19066694133903184 1103 8 0.3107875284823294 1103 9 0.3036855871759059 1103 10-11 0.3141902719835272 1103 12-13 0.28678874503042806 1103 14-15 0.29906431656687005 1103 16-17 0.3126678431856007 1103 18-19 0.35025915959743514 1103 20-21 0.3260731345415522 1103 22-23 0.3412519097334936 1103 24-25 0.3180050295168684 1103 26-27 0.3978460339686336 1103 28-29 0.3856606876496329 1103 30-31 0.34823138662897435 1103 32-33 0.4287002822804382 1103 34-35 0.4689464904287206 1103 36-37 0.4129386533493786 1103 38-39 0.42196034094116186 1103 40-41 0.3439128631508197 1103 42-43 0.42782355413647366 1103 44-45 0.4624161930497337 1103 46-47 0.40909675323830896 1103 48-49 0.4554239366236885 1103 50-51 0.4625963559731474 1103 52-53 0.3947351135951962 1103 54-55 0.3782470921003238 1103 56-57 0.3981373179914627 1103 58-59 0.32368805842769177 1103 60-61 0.2829811811462193 1103 62-63 0.34155593705437326 1103 64-65 0.2739407816298325 1103 66-67 0.33217296137301133 1103 68-69 0.24503113683678635 1103 70-71 0.32818463052146285 1103 72-73 0.2960330961601656 1103 74-75 0.24014233435198662 1103 76-77 0.2779817151562156 1103 78-79 0.2733110630386406 1103 80-81 0.2589316565298958 1103 82-83 0.2233412683691185 1103 84-85 0.2145053415675875 1103 86-87 0.16128577618075468 1103 88-89 0.06932848673259429 1103 90-91 0.17426069282861079 1103 92-93 0.19294285461879213 1103 94-95 0.15257250297581493 1103 96-97 0.26792374567449073 1103 98-99 0.2284988738706133 1103 100 0.18569586197356358 1104 1 0.03485986882750325 1104 2 0.12268648191790277 1104 3 0.1191937187358505 1104 4 0.09700091575217584 1104 5 0.17536193727430316 1104 6 0.14267102223187322 1104 7 0.09865082558174265 1104 8 0.13154927129824046 1104 9 0.17618460031750516 1104 10-11 0.21760823808034502 1104 12-13 0.17471950672530312 1104 14-15 0.27760010636523447 1104 16-17 0.2473264700860085 1104 18-19 0.1845657137755552 1104 20-21 0.1851384877409714 1104 22-23 0.23159116528267276 1104 24-25 0.28313408822175745 1104 26-27 0.2788380224363749 1104 28-29 0.21793543222511147 1104 30-31 0.18664119416362013 1104 32-33 0.2426751561463476 1104 34-35 0.28959995849686493 1104 36-37 0.2761106160229545 1104 38-39 0.343075754630604 1104 40-41 0.37846193678244333 1104 42-43 0.29359155371456325 1104 44-45 0.30721005550963554 1104 46-47 0.22332309620475854 1104 48-49 0.24101444552299967 1104 50-51 0.2804042358177412 1104 52-53 0.37454670754331687 1104 54-55 0.38921408548238645 1104 56-57 0.3552012264621496 1104 58-59 0.3338850625333407 1104 60-61 0.42536599039888046 1104 62-63 0.3553920913910318 1104 64-65 0.36365276280999836 1104 66-67 0.43374418378292745 1104 68-69 0.360594892684027 1104 70-71 0.34051252960779976 1104 72-73 0.3568209367861286 1104 74-75 0.3499357253572697 1104 76-77 0.2864969846924126 1104 78-79 0.2306709491224126 1104 80-81 0.21426372838917018 1104 82-83 0.27527351314835613 1104 84-85 0.32249338100356795 1104 86-87 0.18527922056428991 1104 88-89 0.27671817833734025 1104 90-91 0.2989287967659777 1104 92-93 0.31631501770054626 1104 94-95 0.2649910172385148 1104 96-97 0.29893579094052924 1104 98-99 0.2649647701218285 1104 100 0.2614567018340104 1105 1 -0.06496568996878338 1105 2 -0.029386150171607284 1105 3 -0.010842442686385567 1105 4 -0.015417021564957167 1105 5 -0.09323533271778928 1105 6 -0.03628368656084291 1105 7 -0.06289350981905528 1105 8 -0.03563709884905819 1105 9 -0.07304742581124657 1105 10-11 -0.06967304698008547 1105 12-13 -0.0503812816961684 1105 14-15 0.023424071076938446 1105 16-17 -0.0742497224632146 1105 18-19 0.028302934024608817 1105 20-21 -0.05326881130113037 1105 22-23 -0.010394080537999173 1105 24-25 0.004572639114201138 1105 26-27 0.07053625019841547 1105 28-29 0.09257867296593503 1105 30-31 0.045605511641703345 1105 32-33 0.059904891162005924 1105 34-35 0.05614717619647536 1105 36-37 0.09742626655273767 1105 38-39 0.07869378913903802 1105 40-41 0.06482341724401408 1105 42-43 0.0812102318699317 1105 44-45 0.025823830842504947 1105 46-47 -0.018445401971824538 1105 48-49 0.012479518473227813 1105 50-51 0.03548614740617495 1105 52-53 0.14503910051400482 1105 54-55 0.1249779989784443 1105 56-57 0.03579711221451731 1105 58-59 0.09973134013415574 1105 60-61 0.014205296794145283 1105 62-63 0.08915710994727277 1105 64-65 0.10143420878215892 1105 66-67 0.03694559097060335 1105 68-69 0.04702455738323863 1105 70-71 -0.036700236813096865 1105 72-73 -0.009471153750268968 1105 74-75 0.07459128374552648 1105 76-77 0.04633629998461686 1105 78-79 0.035261366636973435 1105 80-81 -0.02337904817729708 1105 82-83 0.005532834140723253 1105 84-85 0.08336243934933663 1105 86-87 0.005401455191083926 1105 88-89 -0.052398009751136954 1105 90-91 -0.0217774906950865 1105 92-93 0.020110417741832975 1105 94-95 -0.00160237246454642 1105 96-97 -0.0711250702026156 1105 98-99 -0.04132687863592821 1105 100 -0.10357409535247086 1106 1 0.03825676163310021 1106 2 0.13976622373307634 1106 3 0.09727128863693935 1106 4 0.09990359248872238 1106 5 0.09400024991068534 1106 6 0.027796871305262982 1106 7 0.07072784239228014 1106 8 0.11493872764258839 1106 9 0.020591929036953616 1106 10-11 0.13229057504671715 1106 12-13 0.17433150441586776 1106 14-15 0.19969321253794448 1106 16-17 0.11863689021011226 1106 18-19 0.12888618390852002 1106 20-21 0.15588588505443823 1106 22-23 0.18888087477230897 1106 24-25 0.2071648090161915 1106 26-27 0.23123171205070747 1106 28-29 0.17554393280141767 1106 30-31 0.1786011571191395 1106 32-33 0.2137354271367542 1106 34-35 0.23233254041403484 1106 36-37 0.26777927763071574 1106 38-39 0.24703709342134061 1106 40-41 0.2607606871753063 1106 42-43 0.22832919074754443 1106 44-45 0.15787927709910576 1106 46-47 0.16560191017008918 1106 48-49 0.2562755950465032 1106 50-51 0.16433132254358895 1106 52-53 0.26087468964067995 1106 54-55 0.2022994408969936 1106 56-57 0.22540351798281222 1106 58-59 0.18800713015720305 1106 60-61 0.22728054959379307 1106 62-63 0.20315586479888026 1106 64-65 0.2423350021016617 1106 66-67 0.19628429857767316 1106 68-69 0.31625941180534767 1106 70-71 0.33906576037868774 1106 72-73 0.2674742667597769 1106 74-75 0.211889072798499 1106 76-77 0.2064224463028097 1106 78-79 0.32560058288119365 1106 80-81 0.28361464696590843 1106 82-83 0.2154146441196545 1106 84-85 0.20277968775789645 1106 86-87 0.2743417051153543 1106 88-89 0.24453855514001788 1106 90-91 0.21391149845452162 1106 92-93 0.19538428555067355 1106 94-95 0.17002835085068568 1106 96-97 0.1788740191751721 1106 98-99 0.1609006781326272 1106 100 0.25894577260293516 1107 1 0.058857306256118846 1107 2 0.07164319069499925 1107 3 0.08736826347359283 1107 4 0.043736246190533734 1107 5 0.06320798587039889 1107 6 -0.048218514708004534 1107 7 -0.018563624537982548 1107 8 -0.05611241130236522 1107 9 -0.055141095548172814 1107 10-11 -0.07023085330837375 1107 12-13 -0.017945132688431897 1107 14-15 0.09848419349265924 1107 16-17 0.12044773421058608 1107 18-19 0.09799080494396151 1107 20-21 0.11191445986576554 1107 22-23 0.12145465907626374 1107 24-25 0.11248321248328352 1107 26-27 0.11815841922910408 1107 28-29 0.06214587615291833 1107 30-31 0.0624102554624173 1107 32-33 0.05262338292811819 1107 34-35 0.02400494333594594 1107 36-37 0.024474657696629265 1107 38-39 -0.012152731654992976 1107 40-41 -0.030488655449843804 1107 42-43 4.2889420608815954E-4 1107 44-45 -0.008727457172767572 1107 46-47 0.03347844484782314 1107 48-49 0.01406483309742157 1107 50-51 0.010967972533819648 1107 52-53 -0.03572024630140902 1107 54-55 0.006905539132887384 1107 56-57 0.06064190757275156 1107 58-59 -0.04527462276897154 1107 60-61 0.014106619910187135 1107 62-63 -0.025021630540983608 1107 64-65 -0.06492599567634016 1107 66-67 -0.11025276084350111 1107 68-69 -0.04127906011132154 1107 70-71 0.04062790329680155 1107 72-73 -0.01262909597072337 1107 74-75 -0.02976212892401975 1107 76-77 -0.045839680370228564 1107 78-79 -0.005181424734715279 1107 80-81 0.022243645250540567 1107 82-83 2.343788568524019E-4 1107 84-85 -0.06242962590145851 1107 86-87 -0.020604837574772716 1107 88-89 0.011170414209864532 1107 90-91 -0.04602085044562898 1107 92-93 -0.046378841984179076 1107 94-95 -0.040594895576084866 1107 96-97 -0.094551103420077 1107 98-99 -0.02447737779999315 1107 100 0.054278893006475926 1108 1 0.16736833939370044 1108 2 0.1490849452573748 1108 3 0.17293516116535557 1108 4 0.1658094968532069 1108 5 0.2004754581855508 1108 6 0.13579890035539677 1108 7 0.16506711083781767 1108 8 0.168837378248071 1108 9 0.1900063244375616 1108 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0.11016352854928613 2214 64-65 0.05532548625397027 2214 66-67 0.09438678485444285 2214 68-69 0.1363719746564982 2214 70-71 0.08405362829186913 2214 72-73 0.10196145913077714 2214 74-75 0.06077559336551985 2214 76-77 0.08944599633880301 2214 78-79 0.006637145660498334 2214 80-81 -0.006582600181545217 2214 82-83 0.13080723534212524 2214 84-85 0.12698290967895787 2214 86-87 -0.005747957754387301 2214 88-89 0.017254783250429284 2214 90-91 0.13378450235241957 2214 92-93 0.06810508928806769 2214 94-95 -0.0018286788901562545 2214 96-97 -0.048766032635004564 2214 98-99 -0.09233921774212206 2214 100 -0.05847275332181212 2215 1 -0.07386965009155944 2215 2 -0.10846703760906351 2215 3 -0.10461152421295594 2215 4 -0.023472653050426118 2215 5 -0.03071888040215498 2215 6 -0.06658110350365831 2215 7 -0.065292373289779 2215 8 -0.04069625737397331 2215 9 -0.1293330920543525 2215 10-11 -0.0710580205137461 2215 12-13 -0.1332307800351984 2215 14-15 -0.1977055829799852 2215 16-17 -0.2455105196056735 2215 18-19 -0.16257835160251943 2215 20-21 -0.19484219852885332 2215 22-23 -0.10613136153638436 2215 24-25 -0.11611342380198408 2215 26-27 -0.22082034260178318 2215 28-29 -0.17840635718715703 2215 30-31 -0.12385084186194462 2215 32-33 -0.21742527129028133 2215 34-35 -0.15554680778373609 2215 36-37 -0.2196325294344561 2215 38-39 -0.20635420197228882 2215 40-41 -0.2013663534388428 2215 42-43 -0.2079379140180464 2215 44-45 -0.19907804504948245 2215 46-47 -0.1910554845887802 2215 48-49 -0.16139583523275292 2215 50-51 -0.1805669554629361 2215 52-53 -0.2154154124968528 2215 54-55 -0.268044305396522 2215 56-57 -0.2605675444019653 2215 58-59 -0.3085917531936673 2215 60-61 -0.23630110653436276 2215 62-63 -0.23848172111652843 2215 64-65 -0.27959413933252364 2215 66-67 -0.2617240448462965 2215 68-69 -0.23096983628144585 2215 70-71 -0.3208832889931301 2215 72-73 -0.27939228253769954 2215 74-75 -0.2562705461720469 2215 76-77 -0.35242227312373586 2215 78-79 -0.29446981982789566 2215 80-81 -0.28018713223886493 2215 82-83 -0.37870936512960185 2215 84-85 -0.3087375122521223 2215 86-87 -0.31242153468349443 2215 88-89 -0.17695758118630067 2215 90-91 -0.18581142576640985 2215 92-93 -0.23354796941546851 2215 94-95 -0.31200110257146463 2215 96-97 -0.26605312897320843 2215 98-99 -0.3130462522930344 2215 100 -0.32906437285391377 2216 1 -0.017690247943683346 2216 2 -0.1433540856967639 2216 3 -0.1490136953454524 2216 4 -0.08072720298693525 2216 5 -0.032892896578673 2216 6 -0.036332688365263266 2216 7 -0.052151161306689175 2216 8 -0.08069569924222719 2216 9 -0.04137162174928477 2216 10-11 -0.15395021271571352 2216 12-13 -0.1711682506751444 2216 14-15 -0.25708721951529157 2216 16-17 -0.34788923060040133 2216 18-19 -0.2878224644753544 2216 20-21 -0.32446532006669315 2216 22-23 -0.3067831663949221 2216 24-25 -0.351432791438711 2216 26-27 -0.3510800599080568 2216 28-29 -0.4042339037795344 2216 30-31 -0.3826256961663006 2216 32-33 -0.4553001381526087 2216 34-35 -0.46448454353625834 2216 36-37 -0.43554658644761446 2216 38-39 -0.4172540498813859 2216 40-41 -0.3528053566155478 2216 42-43 -0.3229380302954894 2216 44-45 -0.26967636228226155 2216 46-47 -0.2191215115744498 2216 48-49 -0.21835155212972523 2216 50-51 -0.19608018085979495 2216 52-53 -0.26602256681562864 2216 54-55 -0.28730352503531975 2216 56-57 -0.23616221052289887 2216 58-59 -0.203600148425366 2216 60-61 -0.25623110751109834 2216 62-63 -0.2712336362060839 2216 64-65 -0.2699581342535282 2216 66-67 -0.28722792153639176 2216 68-69 -0.2851547546314208 2216 70-71 -0.39478662987674795 2216 72-73 -0.3732326475558665 2216 74-75 -0.40013635269568226 2216 76-77 -0.3342267595727577 2216 78-79 -0.25127195747248265 2216 80-81 -0.2630663013667416 2216 82-83 -0.30963870099933644 2216 84-85 -0.43738060095320463 2216 86-87 -0.3713293173655998 2216 88-89 -0.3635049316883894 2216 90-91 -0.2958672389411099 2216 92-93 -0.3725957501441144 2216 94-95 -0.35614397499649897 2216 96-97 -0.37559587953947826 2216 98-99 -0.39920556169801813 2216 100 -0.4401529623154552 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24433.0 3 3607.0 4 2340.0 5 2008.0 6 1618.0 7 1811.0 8 2082.0 9 2126.0 10 2329.0 11 2603.0 12 2741.0 13 2946.0 14 3275.0 15 3393.0 16 3320.0 17 3318.0 18 3325.0 19 3292.0 20 3264.0 21 3397.0 22 4189.0 23 5652.0 24 8781.0 25 12357.0 26 13122.0 27 11328.0 28 11419.0 29 14508.0 30 17552.0 31 21233.0 32 27677.0 33 37169.0 34 54149.0 35 85298.0 36 161468.0 37 352971.0 38 591574.0 39 268500.0 40 520.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70574856114176 19.492431419774853 13.050942735188126 23.750877283895264 2 15.635704086317077 19.850674387362243 40.38742420359204 24.126197322728643 3 18.468991156424266 25.242212583788653 29.52492536472709 26.763870895059988 4 12.462322484048842 16.524762809792126 38.67702982600324 32.33588488015579 5 13.05235372944912 37.95691856368175 35.40843209190166 13.58229561496748 6 31.121633359357308 37.99410348529758 17.722834517931197 13.161428637413916 7 27.90657589525613 32.46360337565102 21.94274551597418 17.687075213118668 8 25.415772222444406 36.54385062673526 20.076932999344905 17.963444151475432 9 25.02824852392309 17.35490172442734 20.875293046751167 36.7415567048984 10-11 23.98541673414267 27.123945086033665 28.528647611912767 20.3619905679109 12-13 24.568639572364205 24.365946289237147 28.611098985125405 22.454315153273242 14-15 21.899616361443734 26.418479763925134 25.67553562104554 26.006368253585592 16-17 21.742994858883318 29.829375757754978 26.122899584176455 22.30472979918525 18-19 21.65278624221444 28.795309456675167 28.022188777527568 21.52971552358283 20-21 22.7897578299036 28.10201052717549 27.537773071496364 21.57045857142455 22-23 22.394102177183647 27.81903685266201 27.676418453198693 22.11044251695565 24-25 22.229432017404896 27.898302459356895 27.60357391800266 22.26869160523555 26-27 21.98922032985633 27.922713966989583 27.67445711591722 22.413608587236865 28-29 22.087335851012 27.948824097620594 27.75937863465216 22.204461416715247 30-31 22.26668277610722 28.363570922234494 27.395821627937828 21.97392467372046 32-33 21.960724895148854 28.04380936073188 27.87305660666429 22.122409137454984 34-35 22.046871110079742 27.767398398803213 27.94546873926312 22.240261751853925 36-37 22.375276510121925 28.181207590634067 27.367905253657117 22.07561064558689 38-39 22.154758944414983 28.310826851648724 27.53206719530215 22.00234700863414 40-41 21.953138003198706 27.973597002823862 27.858102195865786 22.215162798111642 42-43 21.88784801413602 27.97882413510105 27.774157153374347 22.35917069738859 44-45 21.99208642799024 28.09861632187605 27.781487626488865 22.127809623644843 46-47 22.05858288332515 28.25969703908558 27.627080310864045 22.054639766725224 48-49 21.938957726358105 28.098869082625242 27.677901482427856 22.284271708588797 50-51 21.937136409496066 28.02486737942188 27.731882510870747 22.306113700211302 52-53 22.031173334850557 27.928746019397565 27.780450446745693 22.259630199006182 54-55 22.058322334380556 28.390818000990325 27.525773602400673 22.025086062228443 56-57 21.947533263482836 28.102629533240954 27.64854496907426 22.30129223420196 58-59 21.845820715264257 27.686353107650152 27.956345987454036 22.511480189631552 60-61 22.092370226709303 28.13987626663903 27.54700836887255 22.22074513777912 62-63 21.9110378448684 28.0984114725698 27.657884575249657 22.33266610731214 64-65 21.88906911120606 27.888106444263755 27.810231251300856 22.412593193229334 66-67 21.802501855338516 27.92868177318258 27.663371885882952 22.605444485595953 68-69 22.191576263749138 27.970734971635668 27.665630405797803 22.172058358817388 70-71 22.1049477221597 27.83210595035717 27.618398239868597 22.44454808761453 72-73 21.878055787125703 27.946708199085236 27.810591893292475 22.36464412049659 74-75 21.862263800755745 27.88438872017207 27.80060140982359 22.45274606924859 76-77 21.884485896448723 27.91414343111968 27.639941351847796 22.5614293205838 78-79 22.010320369690266 27.972105373441693 27.72831888714398 22.289255369724064 80-81 21.827362845127997 27.828202702470538 27.83110361817415 22.513330834227315 82-83 21.81175683984058 27.868849227081043 27.964916658475637 22.35447727460274 84-85 21.708205399443823 27.77152542258317 27.86297315824486 22.65729601972815 86-87 21.922360366525986 27.882054900039005 27.741685642145704 22.453899091289305 88-89 21.72919128011524 27.7561969564048 27.825647365967892 22.688964397512066 90-91 21.73297874767201 27.63793961435852 27.911116643493557 22.71796499447591 92-93 21.890742860232688 27.84605003469625 27.743369411425427 22.51983769364563 94-95 21.71702649505902 27.572164459237925 27.91856400210318 22.792245043599873 96-97 21.73171640456488 27.561593050897432 27.935785461891204 22.77090508264648 98-99 21.724211832447494 27.61497697883585 27.890543791748655 22.770267396968006 100 21.798035313883982 27.582769203733815 27.78012773106972 22.839067751312488 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1767.0 1 1123.0 2 442.0 3 362.0 4 280.5 5 226.0 6 200.5 7 191.5 8 194.5 9 202.0 10 207.5 11 206.5 12 214.5 13 220.5 14 233.0 15 267.0 16 355.5 17 509.5 18 677.0 19 846.0 20 1146.0 21 1670.5 22 2457.0 23 3694.5 24 5363.0 25 7415.0 26 10087.0 27 13425.0 28 17534.5 29 22392.5 30 27016.0 31 31734.0 32 36672.5 33 41530.5 34 46347.5 35 50194.5 36 53173.5 37 56585.5 38 59570.5 39 60545.0 40 61685.5 41 63123.0 42 63174.0 43 63244.5 44 62991.0 45 62093.0 46 61380.0 47 60435.5 48 59625.0 49 58671.5 50 57345.0 51 55359.5 52 52049.5 53 49380.0 54 47703.0 55 45065.0 56 41467.5 57 37738.0 58 34113.5 59 31442.5 60 28249.5 61 23985.0 62 20440.5 63 17459.0 64 14441.0 65 11447.0 66 8920.0 67 6774.0 68 5115.5 69 3953.0 70 2967.0 71 2150.5 72 1708.5 73 1315.5 74 952.5 75 676.0 76 451.0 77 324.5 78 231.5 79 157.0 80 120.0 81 86.5 82 63.5 83 50.5 84 40.0 85 28.5 86 22.0 87 20.0 88 15.0 89 10.5 90 9.0 91 7.5 92 3.0 93 2.0 94 3.0 95 2.5 96 2.0 97 2.0 98 1.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07480180897677992 2 0.07767230728965861 3 0.07851657149932881 4 0.08059908988318197 5 0.07913569858642029 6 0.07930455142835433 7 0.07840400293803944 8 0.07789744441223732 9 0.07772859157030329 10-11 0.07725017518482351 12-13 0.07612448957192991 14-15 0.07463295613484588 16-17 0.0733102755396959 18-19 0.07277557487357143 20-21 0.07260672203163739 22-23 0.07474552469613524 24-25 0.0749988039590363 26-27 0.07407011332839908 28-29 0.07035535080585019 30-31 0.07533650964290438 32-33 0.07435153473162248 34-35 0.07164988926067783 36-37 0.06852611168489808 38-39 0.06998950298165978 40-41 0.07263486417195973 42-43 0.07722203304450116 44-45 0.07826329223642775 46-47 0.08158406479446388 48-49 0.08437013668637555 50-51 0.08543953801862447 52-53 0.08577724370249255 54-55 0.08588981226378191 56-57 0.08301931395090323 58-59 0.08234390258316707 60-61 0.08026138419931389 62-63 0.08073980058479369 64-65 0.0798392520944788 66-67 0.0795015464106107 68-69 0.07891056146384157 70-71 0.07727831732514585 72-73 0.07739088588643521 74-75 0.07691246950095543 76-77 0.07665919023805436 78-79 0.07716574876385648 80-81 0.0784321450783618 82-83 0.07764416514933627 84-85 0.07677175879934373 86-87 0.07753159658804691 88-89 0.0747173825558129 90-91 0.07257857989131505 92-93 0.07243786918970335 94-95 0.07252229561067038 96-97 0.07156546283971081 98-99 0.07111518859455336 100 0.07013021368327146 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1776695.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.67149776172324 #Duplication Level Percentage of deduplicated Percentage of total 1 82.56001912586596 59.17200225987331 2 11.254474399818323 16.132500735119013 3 3.0007550380676364 6.452058239830332 4 1.1238409185372147 3.2218944750989196 5 0.5885682376995484 2.1091783565452284 6 0.352382912948686 1.515348669400276 7 0.2357382733116951 1.182700058960816 8 0.1590829528552485 0.9121370799594615 9 0.12023181134563733 0.7755474597972102 >10 0.5812885596106673 7.128926500481031 >50 0.01992043510897317 0.9416895219771515 >100 0.0035401078437941286 0.3463159105886321 >500 7.861349329682104E-5 0.04406059204531925 >1k 7.861349329682104E-5 0.06564014032326973 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 5.628428064468015E-5 0.0 0.0 0.0 0.0 10-11 8.442642096702022E-5 0.0 0.0 0.0 0.0 12-13 1.125685612893603E-4 0.0 0.0 0.0 0.0 14-15 1.6885284193404046E-4 2.8142140322340074E-5 0.0 0.0 0.0 16-17 1.6885284193404046E-4 5.628428064468015E-5 0.0 2.8142140322340074E-5 0.0 18-19 1.6885284193404046E-4 5.628428064468015E-5 0.0 1.125685612893603E-4 0.0 20-21 1.6885284193404046E-4 5.628428064468015E-5 0.0 1.125685612893603E-4 0.0 22-23 1.9699498225638054E-4 5.628428064468015E-5 0.0 2.251371225787206E-4 0.0 24-25 2.251371225787206E-4 5.628428064468015E-5 0.0 4.502742451574412E-4 0.0 26-27 2.251371225787206E-4 5.628428064468015E-5 0.0 4.784163854797813E-4 0.0 28-29 2.251371225787206E-4 5.628428064468015E-5 0.0 0.0013789648757946637 0.0 30-31 2.251371225787206E-4 5.628428064468015E-5 0.0 0.005909849467691415 0.0 32-33 2.251371225787206E-4 5.628428064468015E-5 0.0 0.015562603598254062 0.0 34-35 2.251371225787206E-4 5.628428064468015E-5 0.0 0.027748150357827316 0.0 36-37 2.251371225787206E-4 5.628428064468015E-5 0.0 0.050655852580212136 0.0 38-39 2.251371225787206E-4 5.628428064468015E-5 0.0 0.0928409209233999 0.0 40-41 2.251371225787206E-4 5.628428064468015E-5 0.0 0.14383447918748013 0.0 42-43 2.251371225787206E-4 5.628428064468015E-5 0.0 0.1964321394499337 0.0 44-45 2.251371225787206E-4 5.628428064468015E-5 0.0 0.2542923799526649 0.0 46-47 2.251371225787206E-4 5.628428064468015E-5 0.0 0.32658953844075655 0.0 48-49 2.251371225787206E-4 5.628428064468015E-5 0.0 0.4107063958642311 0.0 50-51 2.251371225787206E-4 5.628428064468015E-5 0.0 0.5060801094166416 0.0 52-53 2.251371225787206E-4 5.628428064468015E-5 0.0 0.6059002811399818 0.0 54-55 2.251371225787206E-4 5.628428064468015E-5 0.0 0.7101669110342519 0.0 56-57 2.251371225787206E-4 5.628428064468015E-5 0.0 0.8328947849799768 0.0 58-59 2.251371225787206E-4 5.628428064468015E-5 0.0 0.9710726939626666 0.0 60-61 2.251371225787206E-4 5.628428064468015E-5 0.0 1.127430425593588 0.0 62-63 2.251371225787206E-4 5.628428064468015E-5 0.0 1.2805518110874403 0.0 64-65 2.251371225787206E-4 5.628428064468015E-5 0.0 1.441637422292515 0.0 66-67 2.251371225787206E-4 5.628428064468015E-5 0.0 1.6290640768392999 0.0 68-69 2.251371225787206E-4 5.628428064468015E-5 0.0 1.8378224737504185 0.0 70-71 2.251371225787206E-4 5.628428064468015E-5 0.0 2.0648169775904135 0.0 72-73 2.251371225787206E-4 5.628428064468015E-5 0.0 2.309541029833483 0.0 74-75 2.8142140322340076E-4 5.628428064468015E-5 0.0 2.5460475771024287 0.0 76-77 2.8142140322340076E-4 5.628428064468015E-5 0.0 2.7958653567438416 0.0 78-79 2.8142140322340076E-4 5.628428064468015E-5 0.0 3.083281035855901 0.0 80-81 2.8142140322340076E-4 5.628428064468015E-5 0.0 3.404298430512834 0.0 82-83 2.8142140322340076E-4 5.628428064468015E-5 0.0 3.726863642887496 0.0 84-85 2.8142140322340076E-4 5.628428064468015E-5 0.0 4.042871736567053 0.0 86-87 2.8142140322340076E-4 5.628428064468015E-5 0.0 4.375286698054534 0.0 88 2.8142140322340076E-4 5.628428064468015E-5 0.0 4.644015995992559 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2650 0.0 34.58501 1 TCAACGC 2615 0.0 33.070843 4 GGTATCA 1665 0.0 32.462563 1 ATCAACG 2725 0.0 31.908354 3 CAACGCA 2715 0.0 31.50654 5 AACGCAG 2905 0.0 29.607662 6 TATCAAC 3195 0.0 27.50869 2 GTACATG 5480 0.0 26.073072 1 TACATGG 5600 0.0 24.759003 2 ACGCAGA 3425 0.0 24.42635 7 CGCAGAG 3545 0.0 23.334343 8 ACATGGG 5610 0.0 23.039286 3 GCAGAGT 4165 0.0 19.860804 9 GTATTAG 965 0.0 19.481922 1 CATGGGG 3780 0.0 19.148205 4 TATACCG 130 0.008040461 18.076979 5 GAGTACA 4245 0.0 17.604292 1 CATGGGA 1965 0.0 17.221426 4 ACCGTTC 250 5.0465987E-6 16.920052 8 GTACCGG 175 0.0023292322 16.114334 1 >>END_MODULE