FastQCFastQC Report
Wed 25 May 2016
SRR1032983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032983_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776695
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41430.23318577471090987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33870.19063485854353168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18530.10429477203459231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25350.053.7483751
GTATCAA50350.046.6568951
ATCAACG61100.037.5612223
TCAACGC61000.037.54574
CAACGCA63600.036.0067565
TATCAAC65100.035.4700362
AACGCAG66050.034.813546
ACGCAGA77250.029.5226547
CGCAGAG78400.029.0296278
GTACATG57350.026.6253431
GCAGAGT86050.026.2051569
TACATGG56450.025.3262422
ACATGGG58200.024.0798823
GTGGTAT13150.021.7946241
AGAGTAC79150.021.39689610-11
GAGTACT57650.021.35002512-13
ATGTCGC1805.6434183E-620.8991996
GTTCGGA1354.0879406E-420.8815541
CAGAGTA84000.020.86055810-11
CTGTCGC4100.020.6245779
Produced by FastQC (version 0.11.5)