##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032983_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1776695
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84784445276201	34.0	31.0	34.0	31.0	34.0
2	32.89393565018194	34.0	31.0	34.0	31.0	34.0
3	32.968299567455304	34.0	31.0	34.0	31.0	34.0
4	36.377032636440134	37.0	37.0	37.0	35.0	37.0
5	36.35686597868514	37.0	37.0	37.0	35.0	37.0
6	36.42093043544334	37.0	37.0	37.0	35.0	37.0
7	36.38706362093663	37.0	37.0	37.0	35.0	37.0
8	36.32209636431689	37.0	37.0	37.0	35.0	37.0
9	38.16536940780494	39.0	39.0	39.0	37.0	39.0
10-11	38.1791255674159	39.0	39.0	39.0	37.0	39.0
12-13	38.17486344026409	39.0	39.0	39.0	37.0	39.0
14-15	39.73708599393818	41.0	40.0	41.0	37.0	41.0
16-17	39.75145171230853	41.0	40.0	41.0	37.0	41.0
18-19	39.77681903759509	41.0	40.0	41.0	37.0	41.0
20-21	39.7886587174501	41.0	40.0	41.0	37.0	41.0
22-23	39.753516782565384	41.0	40.0	41.0	37.0	41.0
24-25	39.73850857913148	41.0	40.0	41.0	37.0	41.0
26-27	39.6676531424921	41.0	40.0	41.0	37.0	41.0
28-29	39.60459110877218	41.0	40.0	41.0	37.0	41.0
30-31	39.495482060792654	41.0	40.0	41.0	36.5	41.0
32-33	39.37119567511588	41.0	39.0	41.0	35.5	41.0
34-35	39.28298498053971	41.0	39.0	41.0	35.0	41.0
36-37	39.19588871472031	41.0	39.0	41.0	35.0	41.0
38-39	39.09786007164989	41.0	39.0	41.0	35.0	41.0
40-41	38.99466002887384	41.0	39.0	41.0	35.0	41.0
42-43	38.86217161640012	41.0	39.0	41.0	35.0	41.0
44-45	38.69424830936092	41.0	39.0	41.0	35.0	41.0
46-47	38.47552168492622	41.0	39.0	41.0	35.0	41.0
48-49	38.27047439205941	41.0	38.0	41.0	34.5	41.0
50-51	38.11634664362764	40.5	38.0	41.0	34.0	41.0
52-53	37.978418074008204	40.0	38.0	41.0	34.0	41.0
54-55	37.83114378100912	40.0	37.5	41.0	34.0	41.0
56-57	37.6255983722586	40.0	37.0	41.0	33.0	41.0
58-59	37.45738914107373	40.0	37.0	41.0	33.0	41.0
60-61	37.267950323493906	40.0	36.0	41.0	33.0	41.0
62-63	37.02482868472079	39.0	36.0	41.0	33.0	41.0
64-65	36.73765390232988	39.0	35.0	41.0	32.5	41.0
66-67	36.462521141782915	39.0	35.0	41.0	32.0	41.0
68-69	36.168435775414466	37.5	35.0	40.5	32.0	41.0
70-71	35.840888278517134	37.0	35.0	40.0	31.5	41.0
72-73	35.496474352660414	37.0	35.0	39.0	31.0	41.0
74-75	35.14741500370069	36.0	35.0	39.0	31.0	41.0
76-77	34.797742437503345	36.0	35.0	39.0	31.0	40.5
78-79	34.452074779295266	35.0	35.0	37.0	31.0	39.5
80-81	34.12176400563968	35.0	34.5	37.0	30.5	39.0
82-83	33.81666183559925	35.0	34.0	36.5	30.0	39.0
84-85	33.53530825493402	35.0	34.0	36.0	30.0	37.0
86-87	33.393653665935915	35.0	34.0	36.0	30.0	37.0
88-89	33.20637194341178	35.0	34.0	35.5	30.0	37.0
90-91	33.03117980294873	35.0	34.0	35.0	30.0	36.0
92-93	32.874495341068666	35.0	34.0	35.0	30.0	36.0
94-95	32.77741339959869	35.0	34.0	35.0	30.0	36.0
96-97	32.71636746881147	35.0	34.0	35.0	30.0	36.0
98-99	32.608545923751684	35.0	34.0	35.0	30.0	36.0
100	32.52467024447078	35.0	34.0	35.0	30.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15645781053598284
1101	2	0.2097940686894333
1101	3	0.1657367032794994
1101	4	0.037956107779656634
1101	5	0.07466886122401206
1101	6	0.06697523193583521
1101	7	0.07645034139230233
1101	8	0.028414952073269717
1101	9	0.10245633465715542
1101	10-11	0.13814675827239853
1101	12-13	0.18674021397379903
1101	14-15	0.25561712321085395
1101	16-17	0.24254780130223708
1101	18-19	0.21200806340295486
1101	20-21	0.24521198799833854
1101	22-23	0.1961508622564665
1101	24-25	0.17834356446175548
1101	26-27	0.24800676971118918
1101	28-29	0.18076527001153408
1101	30-31	0.21096397211396578
1101	32-33	0.2653405584046382
1101	34-35	0.2563777006783141
1101	36-37	0.2147080370381147
1101	38-39	0.14060255161983548
1101	40-41	0.14492294836458797
1101	42-43	0.19255036787331647
1101	44-45	0.26257760214664216
1101	46-47	0.25368177960476856
1101	48-49	0.27037623788810805
1101	50-51	0.2550708292233992
1101	52-53	0.26260508010275174
1101	54-55	0.23215740338256552
1101	56-57	0.24696159283708852
1101	58-59	0.294877549790435
1101	60-61	0.3505331126756701
1101	62-63	0.34158557543010915
1101	64-65	0.3707374289906582
1101	66-67	0.35812086133639554
1101	68-69	0.35238040013786076
1101	70-71	0.3687377341975946
1101	72-73	0.4065471633424025
1101	74-75	0.42048706604868613
1101	76-77	0.3418940866947153
1101	78-79	0.3329412569724326
1101	80-81	0.3563873886962554
1101	82-83	0.37604320404020797
1101	84-85	0.33671541893161105
1101	86-87	0.28316385424876245
1101	88-89	0.25251658018426326
1101	90-91	0.32024905925834446
1101	92-93	0.29581185845874813
1101	94-95	0.23720089252709897
1101	96-97	0.2614036090561882
1101	98-99	0.2469373029394859
1101	100	0.25738283005556895
1102	1	0.2281389621005232
1102	2	0.2599900629087486
1102	3	0.2334662788807904
1102	4	0.14403405000920344
1102	5	0.10164428120234703
1102	6	0.12861725392771461
1102	7	0.09556988704245839
1102	8	0.04152903049347145
1102	9	0.11627732573762728
1102	10-11	0.15204637217200911
1102	12-13	0.2165334134462995
1102	14-15	0.2675263155865224
1102	16-17	0.2938173260411503
1102	18-19	0.2122592581147913
1102	20-21	0.25427915595764006
1102	22-23	0.2498372593830922
1102	24-25	0.2286408678202818
1102	26-27	0.292473861991688
1102	28-29	0.3118664830369511
1102	30-31	0.3108961729207991
1102	32-33	0.30144159392258274
1102	34-35	0.2556600509810707
1102	36-37	0.3095749540624695
1102	38-39	0.31619129193160234
1102	40-41	0.2753499793747025
1102	42-43	0.23953572826975034
1102	44-45	0.2798274313498226
1102	46-47	0.3478208294289473
1102	48-49	0.3360729633515902
1102	50-51	0.34130161836964135
1102	52-53	0.3425857880718013
1102	54-55	0.3295380858944341
1102	56-57	0.26634904487760025
1102	58-59	0.35134890917724704
1102	60-61	0.44405062660134575
1102	62-63	0.39241444870796016
1102	64-65	0.4275865762188076
1102	66-67	0.39970445306575897
1102	68-69	0.38034397387403374
1102	70-71	0.3811949484040795
1102	72-73	0.30728672005863444
1102	74-75	0.24277576938695233
1102	76-77	0.3250727864914822
1102	78-79	0.2918048974687295
1102	80-81	0.33493764625525557
1102	82-83	0.3649411943959251
1102	84-85	0.34611049946371253
1102	86-87	0.27410759650446437
1102	88-89	0.3174424037106718
1102	90-91	0.2794251994840451
1102	92-93	0.30091636706180225
1102	94-95	0.31722160461778515
1102	96-97	0.3186651797288391
1102	98-99	0.3433562413409348
1102	100	0.3012069123636181
1103	1	0.17773390786842214
1103	2	0.22612741178333806
1103	3	0.1784633751611011
1103	4	0.1128604193036864
1103	5	0.12643559468088483
1103	6	0.14341390555760825
1103	7	0.15165332645444352
1103	8	0.02489863588090202
1103	9	0.08407243458311342
1103	10-11	0.1063723279064206
1103	12-13	0.19311066118932274
1103	14-15	0.24962942989921544
1103	16-17	0.23310612002957498
1103	18-19	0.25504131488690973
1103	20-21	0.2404210803905329
1103	22-23	0.21505597693622036
1103	24-25	0.19432887340480676
1103	26-27	0.22313362230283218
1103	28-29	0.22845933460700962
1103	30-31	0.27947733921887874
1103	32-33	0.30213262054881085
1103	34-35	0.32738838013969485
1103	36-37	0.2811026625876707
1103	38-39	0.1755034329995837
1103	40-41	0.181499999026137
1103	42-43	0.2157950333714993
1103	44-45	0.2716976028159337
1103	46-47	0.27357828964212416
1103	48-49	0.23506164764393844
1103	50-51	0.2674239500272222
1103	52-53	0.2222937735942594
1103	54-55	0.23247113609065906
1103	56-57	0.20652479324536444
1103	58-59	0.22696822272617823
1103	60-61	0.176001515608867
1103	62-63	0.16902923949349002
1103	64-65	0.16494424995804025
1103	66-67	0.17971278292831272
1103	68-69	0.060504822346786113
1103	70-71	0.18012790474269025
1103	72-73	0.13350349593253696
1103	74-75	0.15537497980984227
1103	76-77	0.13656565038035495
1103	78-79	0.1936527190923556
1103	80-81	0.09744035932668993
1103	82-83	0.15073877873578567
1103	84-85	0.1859501202789815
1103	86-87	0.12718055319503918
1103	88-89	0.03149523381080144
1103	90-91	0.03588758898137456
1103	92-93	0.06739394975915047
1103	94-95	0.16875236351519618
1103	96-97	0.16776150696338732
1103	98-99	0.17649970855823227
1103	100	0.16867762585741275
1104	1	0.10125246160546197
1104	2	0.15391382845653112
1104	3	0.11550692318474631
1104	4	0.061851586321679974
1104	5	0.09137039810029535
1104	6	0.1108312771421609
1104	7	0.08922026901528568
1104	8	0.01967587756771394
1104	9	0.05342788419900302
1104	10-11	0.07310049676068786
1104	12-13	0.13935316785760676
1104	14-15	0.1439591892336196
1104	16-17	0.15367880155159241
1104	18-19	0.18086703968845796
1104	20-21	0.17287873337755855
1104	22-23	0.17164555411334703
1104	24-25	0.18893607179770555
1104	26-27	0.179351069959381
1104	28-29	0.2655584183868882
1104	30-31	0.2133562281201975
1104	32-33	0.2787757997191491
1104	34-35	0.258036526364144
1104	36-37	0.2247727849900798
1104	38-39	0.22271058376757935
1104	40-41	0.24162181011507755
1104	42-43	0.29191106234917896
1104	44-45	0.3429387111018727
1104	46-47	0.2578370590976178
1104	48-49	0.3243004162364329
1104	50-51	0.34362979510564173
1104	52-53	0.33162753629756736
1104	54-55	0.26957349608062486
1104	56-57	0.29808671441494994
1104	58-59	0.3003467784129583
1104	60-61	0.2774078887164535
1104	62-63	0.21946145950138174
1104	64-65	0.2909208402384067
1104	66-67	0.3355167089466917
1104	68-69	0.3450451061464861
1104	70-71	0.33648950564277413
1104	72-73	0.3134820966871885
1104	74-75	0.29463172760399203
1104	76-77	0.274860470931948
1104	78-79	0.27484247877834633
1104	80-81	0.3005984660288519
1104	82-83	0.24127548587296843
1104	84-85	0.28409354796183806
1104	86-87	0.26137875271783173
1104	88-89	0.2422683714451921
1104	90-91	0.22816644080558746
1104	92-93	0.23490840903266275
1104	94-95	0.23971683181825654
1104	96-97	0.1593078461581996
1104	98-99	0.12285951952889462
1104	100	0.18271610614341682
1105	1	0.05877667276214993
1105	2	0.07887603950952382
1105	3	0.11611881727690587
1105	4	0.052291987468954915
1105	5	0.045187503585069067
1105	6	0.09435462130827688
1105	7	0.07000872730294816
1105	8	0.06341012194677376
1105	9	0.0856648395342603
1105	10-11	0.0899981461608661
1105	12-13	0.07896126111953095
1105	14-15	0.1276439053187488
1105	16-17	0.16818938905274905
1105	18-19	0.19858842217334427
1105	20-21	0.1643188120877781
1105	22-23	0.16623668031447636
1105	24-25	0.1423698422771409
1105	26-27	0.08338240740432212
1105	28-29	0.11641433381818445
1105	30-31	0.13540898937474566
1105	32-33	0.15730078674956616
1105	34-35	0.16262008710609877
1105	36-37	0.17833433380462083
1105	38-39	0.13787021771716468
1105	40-41	0.09871056252300292
1105	42-43	0.16325522907643375
1105	44-45	0.0736341351035179
1105	46-47	0.17419877578804943
1105	48-49	0.13656374636993718
1105	50-51	0.11028684388321608
1105	52-53	0.18746051758854776
1105	54-55	0.17931581948651143
1105	56-57	0.21344806326330001
1105	58-59	0.1616076545274936
1105	60-61	0.2058303923066731
1105	62-63	0.1819309836781855
1105	64-65	0.20300436033058844
1105	66-67	0.24041465347954727
1105	68-69	0.19731478991582208
1105	70-71	0.12623117558442942
1105	72-73	0.08515624751348128
1105	74-75	0.09990068949475983
1105	76-77	0.051567830319790176
1105	78-79	0.10364282582001039
1105	80-81	0.11229246928267855
1105	82-83	0.1231164204065891
1105	84-85	0.14411245854186205
1105	86-87	0.17065996967072294
1105	88-89	0.15178366971786517
1105	90-91	0.15486938240704973
1105	92-93	0.1066374447188636
1105	94-95	0.05741426928740623
1105	96-97	0.1052385126825186
1105	98-99	0.08313426289078762
1105	100	0.10667035628399901
1106	1	0.011400714441542448
1106	2	0.05739278599769193
1106	3	0.028788107409738473
1106	4	-0.014148028971781912
1106	5	0.02610945830776501
1106	6	0.049565412866265035
1106	7	0.02732895700120963
1106	8	-0.029466787136264827
1106	9	0.02745509948994851
1106	10-11	0.037264888095926096
1106	12-13	0.00744616497020445
1106	14-15	0.02955932993314292
1106	16-17	0.028653072054808604
1106	18-19	0.08136234935474818
1106	20-21	0.06485679312032033
1106	22-23	0.08924255244358648
1106	24-25	0.09857168220025159
1106	26-27	0.07313783467628099
1106	28-29	0.09123235143297848
1106	30-31	0.12434055851959869
1106	32-33	0.16170681410698506
1106	34-35	0.1963887269963891
1106	36-37	0.13051668728660104
1106	38-39	0.22700512515933724
1106	40-41	0.2990907946486132
1106	42-43	0.15174358017855383
1106	44-45	0.1537938771803411
1106	46-47	0.2004138408596532
1106	48-49	0.2593890530419998
1106	50-51	0.2638532994166596
1106	52-53	0.29407572900078804
1106	54-55	0.28171317451509736
1106	56-57	0.3140003519920356
1106	58-59	0.18684449279471949
1106	60-61	0.24663406230359186
1106	62-63	0.23361964879198638
1106	64-65	0.174611305478642
1106	66-67	0.1225322041709731
1106	68-69	0.22030071577601973
1106	70-71	0.2895408516812097
1106	72-73	0.20697943265434304
1106	74-75	0.19118364545896327
1106	76-77	0.23263660648287754
1106	78-79	0.19177058509637135
1106	80-81	0.24965451183889087
1106	82-83	0.2329233990946804
1106	84-85	0.2962432635216956
1106	86-87	0.23469557922447137
1106	88-89	0.26607957880950295
1106	90-91	0.2585223033407189
1106	92-93	0.22848277619190327
1106	94-95	0.287001579672193
1106	96-97	0.30777123716656263
1106	98-99	0.28772754331479433
1106	100	0.2709136751216903
1107	1	-4.4538687816242373E-4
1107	2	0.02636386701105664
1107	3	0.056314423054296014
1107	4	0.05810194142913616
1107	5	0.031943092220870994
1107	6	0.03934423204206183
1107	7	-0.008451095698916333
1107	8	0.02019302998273531
1107	9	0.008231256125512232
1107	10-11	0.07537796758389703
1107	12-13	0.03165690213866412
1107	14-15	0.07683242891521047
1107	16-17	0.0833440439232973
1107	18-19	0.06524303616347282
1107	20-21	0.09297288758406097
1107	22-23	0.09059901619367139
1107	24-25	0.10508795343242383
1107	26-27	0.08783900790108845
1107	28-29	0.08032784493907741
1107	30-31	0.10020058379583219
1107	32-33	0.107007857371201
1107	34-35	0.09992065407527662
1107	36-37	0.061352042076279645
1107	38-39	-0.009276046798916582
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2216	22-23	-0.4113478189735602
2216	24-25	-0.3672396421662256
2216	26-27	-0.4709773119594587
2216	28-29	-0.42654381864083746
2216	30-31	-0.38692165174180815
2216	32-33	-0.43191795154044144
2216	34-35	-0.45225605362708166
2216	36-37	-0.40483054861987
2216	38-39	-0.3930313578349711
2216	40-41	-0.2883054005354069
2216	42-43	-0.2870540011340452
2216	44-45	-0.26618461586931375
2216	46-47	-0.16985004153898586
2216	48-49	-0.3362474006758305
2216	50-51	-0.28563199759882707
2216	52-53	-0.1898447290634735
2216	54-55	-0.14888085646166616
2216	56-57	-0.17886395716756454
2216	58-59	-0.22944398184452552
2216	60-61	-0.2590262630031077
2216	62-63	-0.1949381469145095
2216	64-65	-0.21430647829205896
2216	66-67	-0.34668380555783074
2216	68-69	-0.38991891410175583
2216	70-71	-0.37363046637956643
2216	72-73	-0.33923742721830763
2216	74-75	-0.2866653217007169
2216	76-77	-0.2679861379616355
2216	78-79	-0.37391417480836964
2216	80-81	-0.3380315767674844
2216	82-83	-0.3173635666799939
2216	84-85	-0.3010398794207063
2216	86-87	-0.23939952428476374
2216	88-89	-0.2892089915413081
2216	90-91	-0.3212738873392311
2216	92-93	-0.38645301261209397
2216	94-95	-0.3734404951292163
2216	96-97	-0.3126153812314527
2216	98-99	-0.33539614417008323
2216	100	-0.30623153057371155
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5.0
3	1.0
4	4.0
5	16.0
6	28.0
7	90.0
8	228.0
9	379.0
10	679.0
11	1055.0
12	1687.0
13	2634.0
14	3146.0
15	4303.0
16	5628.0
17	7846.0
18	9594.0
19	5460.0
20	3613.0
21	3356.0
22	3346.0
23	3704.0
24	4095.0
25	4844.0
26	6154.0
27	7645.0
28	10037.0
29	12835.0
30	16116.0
31	20785.0
32	26609.0
33	34844.0
34	49067.0
35	77357.0
36	146208.0
37	341861.0
38	634126.0
39	325951.0
40	1359.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.531549907609254	19.535170116615532	13.313949005258646	23.61933097051657
2	15.869311814630263	19.96643190387502	40.04830338714698	24.11595289434774
3	18.572524510479887	25.13743260638187	29.905965534767198	26.384077348371044
4	12.468540581313203	16.603987064416167	38.512351568678774	32.41512078559185
5	12.67750704962599	37.66610945072016	35.49381152592743	14.162571973726424
6	30.97836762928737	37.89821590493982	17.46980119247367	13.653615273299138
7	27.557819955095997	32.58174380402988	21.960072697431556	17.90036354344257
8	25.658602627815892	35.86854269566722	19.881432081313378	18.59142259520351
9	25.289854215951312	16.389603240671168	21.065206856201126	37.25533568717639
10-11	24.093949721252102	26.539726852386032	28.493691939246744	20.87263148711512
12-13	25.102388228470467	23.862247483879464	28.89091449932135	22.144449788328714
14-15	22.157989205776357	25.69293141013266	26.438390243435688	25.710689140655298
16-17	21.30939750491784	29.34352829270077	26.883060964318577	22.464013238062808
18-19	21.088157398175202	27.739283944342617	29.79042788238701	21.38213077509517
20-21	22.18923337995548	27.033030430096332	29.547727662879673	21.230008527068517
22-23	21.791430870243932	26.79975673885984	29.72519427261289	21.683618118283338
24-25	21.638454505057588	26.966687707707575	29.611442423860375	21.783415363374463
26-27	21.391505809761345	26.93161970281085	29.726260413975396	21.950614073452403
28-29	21.50676661680418	27.020929581309865	29.74257726692569	21.729726534960268
30-31	21.725526024550252	27.384800370178976	29.32292036085695	21.56675324441382
32-33	21.431138835261073	27.110383167655517	29.79670280645095	21.66177519063246
34-35	21.49124807106259	26.829921907943593	29.841794760496487	21.83703526049733
36-37	21.861696901413527	27.24294412307973	29.240782547714144	21.6545764277926
38-39	21.72156959352455	27.38550765440788	29.310124934013725	21.582797818053844
40-41	21.548590699231568	27.13694135429041	29.45232074330011	21.862147203177912
42-43	21.59024953202361	27.093657515347402	29.29344928803099	22.022643664598004
44-45	21.675648203241916	27.335002298912748	29.157172382394837	21.832177115450495
46-47	21.804626096286096	27.57746975704204	28.768141830287814	21.84976231638405
48-49	21.8731211285034	27.45909237159155	28.62945146390679	22.038335035998262
50-51	21.894421881405286	27.451437812648354	28.50952930354167	22.14461100240469
52-53	21.93059536973778	27.517083512350414	28.373125603082144	22.179195514829658
54-55	21.93963959115835	27.876673559208477	28.269376574805865	21.914310274827308
56-57	21.78245657526297	27.590441205469574	28.37067794986788	22.256424269399574
58-59	21.65320939403542	27.1573254462871	28.75664941375304	22.43281574592444
60-61	21.90682061262333	27.63647098358712	28.315695687727338	22.141012716062214
62-63	21.756991581342565	27.48895732531927	28.426426252172128	22.327624841166045
64-65	21.697562046383876	27.349995356546845	28.569366154573522	22.38307644249576
66-67	21.622519128223544	27.37505726928779	28.406157728905484	22.59626587358318
68-69	22.0112185891324	27.519792378428697	28.34250017166715	22.126488860771747
70-71	21.979321155291146	27.27038124157495	28.347437235991546	22.402860367142363
72-73	21.799633589333002	27.411176369607617	28.42541910682475	22.363770934234633
74-75	21.804417753187803	27.482544837465067	28.263432947129363	22.44960446221777
76-77	21.863928696761707	27.462727814336013	28.124040528093335	22.54930296080894
78-79	21.954893777491353	27.575357616248148	28.180413633178457	22.289334973082042
80-81	21.78996747606436	27.38331331016105	28.30429480863729	22.5224244051373
82-83	21.707148700662213	27.466161158353984	28.370565885449572	22.456124255534228
84-85	21.66457945792609	27.38863451520942	28.21941863966522	22.727367387199266
86-87	21.780241415759452	27.436952843665114	28.17034073550419	22.61246500507124
88-89	21.535029484619987	27.420865441278693	28.17026502668728	22.87384004741404
90-91	21.496223180869407	27.356997703880833	28.220990283355007	22.925788831894753
92-93	21.657630154203257	27.480256725883823	28.066546555065315	22.7955665648476
94-95	21.449422926307193	27.24427787904346	28.24642823874537	23.05987095590398
96-97	21.424826510593977	27.179786496657975	28.27353170369566	23.12185528905239
98-99	21.323221296626965	27.322789596072933	28.172316625058503	23.1816724822416
100	21.46586780511005	27.153000374290464	28.060021556879487	23.321110263719998
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	56.5
2	152.5
3	266.5
4	175.0
5	117.0
6	351.5
7	790.5
8	1300.0
9	1486.5
10	1122.0
11	774.0
12	768.0
13	880.5
14	983.0
15	1103.5
16	1270.5
17	1457.0
18	1623.0
19	1799.0
20	2083.0
21	2552.0
22	3253.5
23	4454.5
24	6055.0
25	7913.0
26	10400.5
27	13725.5
28	17816.5
29	22328.5
30	26918.5
31	31688.5
32	36563.0
33	41441.5
34	45782.5
35	49428.0
36	52738.5
37	56185.0
38	59016.5
39	60117.5
40	61094.5
41	62306.5
42	62854.0
43	63402.5
44	62873.5
45	61946.5
46	61617.0
47	60510.5
48	59390.0
49	58085.5
50	56581.0
51	55080.0
52	51666.0
53	48709.0
54	47211.5
55	44725.5
56	41196.0
57	37471.5
58	34069.0
59	31178.0
60	27863.0
61	23680.5
62	20323.5
63	17314.5
64	14138.5
65	11212.0
66	8562.0
67	6456.0
68	4944.5
69	3766.0
70	2671.0
71	1908.5
72	1499.5
73	1124.0
74	834.0
75	565.0
76	347.5
77	225.5
78	141.5
79	85.5
80	50.5
81	22.5
82	11.0
83	5.0
84	1.5
85	0.5
86	0.5
87	0.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.125685612893603E-4
2	4.502742451574412E-4
3	3.3770568386808086E-4
4	0.01125685612893603
5	2.8142140322340076E-4
6	0.0029267825935233678
7	9.005484903148824E-4
8	0.14194895578588335
9	0.0014633912967616839
10-11	0.0
12-13	8.442642096702022E-5
14-15	2.251371225787206E-4
16-17	0.0
18-19	1.969949822563805E-4
20-21	0.0
22-23	1.969949822563805E-4
24-25	4.784163854797813E-4
26-27	0.006528976554782898
28-29	0.00802050999186692
30-31	0.014465060125682798
32-33	0.017194847736949785
34-35	0.025778200535263508
36-37	0.03802003157548144
38-39	0.04263534258834521
40-41	0.05282279738503232
42-43	0.0693140916139236
44-45	0.10936035729261354
46-47	0.11633960809255388
48-49	0.11403195258612199
50-51	0.1146792218135358
52-53	0.11017647936196139
54-55	0.11659288735545494
56-57	0.10046744095075406
58-59	0.09188408815244034
60-61	0.09323491088791266
62-63	0.0928409209233999
64-65	0.0
66-67	5.628428064468015E-5
68-69	5.628428064468015E-5
70-71	0.0
72-73	0.0
74-75	0.0
76-77	8.442642096702022E-5
78-79	0.0
80-81	1.969949822563805E-4
82-83	8.442642096702022E-5
84-85	0.0
86-87	5.065585258021214E-4
88-89	3.3770568386808086E-4
90-91	8.442642096702022E-5
92-93	7.87979929025522E-4
94-95	8.724063499925424E-4
96-97	8.442642096702022E-5
98-99	2.8142140322340074E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1776695.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.95309812440917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.7777986167907	57.20610371038533
2	11.76740577413801	16.463329815760304
3	3.0874918965953584	6.479388708025597
4	1.208906062565598	3.3826689767137763
5	0.6016340318005768	2.1043082230764827
6	0.3627571579602516	1.5225592239675168
7	0.24623481059549399	1.2057421507062327
8	0.16622505193573164	0.9302365895036196
9	0.1285026454734271	0.8090242351243927
>10	0.62260431490615	7.476182907114525
>50	0.024559910365076612	1.1507444234517517
>100	0.005477191302702399	0.6465046732580965
>500	8.050711417742238E-5	0.03812675833107744
>1k	3.220284567096895E-4	0.5850796045813347
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4143	0.23318577471090987	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3387	0.19063485854353168	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1853	0.10429477203459231	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	5.628428064468015E-5	0.0	0.0	0.0	0.0
6	5.628428064468015E-5	0.0	0.0	0.0	0.0
7	5.628428064468015E-5	0.0	0.0	0.0	0.0
8	5.628428064468015E-5	0.0	0.0	0.0	0.0
9	1.125685612893603E-4	0.0	0.0	0.0	0.0
10-11	1.125685612893603E-4	0.0	0.0	0.0	0.0
12-13	1.125685612893603E-4	0.0	0.0	0.0	0.0
14-15	1.125685612893603E-4	0.0	0.0	0.0	0.0
16-17	1.4071070161170038E-4	0.0	0.0	2.8142140322340074E-5	0.0
18-19	1.6885284193404046E-4	0.0	0.0	5.628428064468015E-5	0.0
20-21	1.6885284193404046E-4	0.0	0.0	5.628428064468015E-5	0.0
22-23	1.6885284193404046E-4	0.0	0.0	1.6885284193404046E-4	0.0
24-25	1.6885284193404046E-4	0.0	0.0	3.939899645127611E-4	0.0
26-27	1.6885284193404046E-4	0.0	0.0	4.2213210483510113E-4	0.0
28-29	1.6885284193404046E-4	0.0	0.0	0.0012945384548276435	0.0
30-31	1.6885284193404046E-4	0.0	0.0	0.005881707327369076	0.0
32-33	1.6885284193404046E-4	0.0	0.0	0.01550631931760938	0.0
34-35	1.9699498225638054E-4	5.628428064468015E-5	0.0	0.027776292498149655	0.0
36-37	2.251371225787206E-4	5.628428064468015E-5	0.0	0.0506277104398898	0.0
38-39	2.251371225787206E-4	5.628428064468015E-5	0.0	0.09267206808146586	0.0
40-41	2.251371225787206E-4	5.628428064468015E-5	0.0	0.14355305778425673	0.0
42-43	2.251371225787206E-4	5.628428064468015E-5	0.0	0.19609443376606564	0.0
44-45	2.251371225787206E-4	5.628428064468015E-5	0.0	0.2540953849704085	0.0
46-47	2.251371225787206E-4	5.628428064468015E-5	0.0	0.32658953844075655	0.0
48-49	2.251371225787206E-4	5.628428064468015E-5	0.0	0.4110722436884215	0.0
50-51	2.251371225787206E-4	5.628428064468015E-5	0.0	0.5068680893456671	0.0
52-53	2.251371225787206E-4	5.628428064468015E-5	0.0	0.6070822510335201	0.0
54-55	2.251371225787206E-4	5.628428064468015E-5	0.0	0.7118272973132699	0.0
56-57	2.251371225787206E-4	5.628428064468015E-5	0.0	0.8355120040299545	0.0
58-59	2.251371225787206E-4	5.628428064468015E-5	0.0	0.9744216086610251	0.0
60-61	2.251371225787206E-4	5.628428064468015E-5	0.0	1.1316236045016168	0.0
62-63	2.251371225787206E-4	5.628428064468015E-5	0.0	1.2856173963454616	0.0
64-65	2.251371225787206E-4	5.628428064468015E-5	0.0	1.4483633938295544	0.0
66-67	2.251371225787206E-4	5.628428064468015E-5	0.0	1.6364091754634307	0.0
68-69	2.251371225787206E-4	1.125685612893603E-4	0.0	1.8463776844084099	0.0
70-71	2.251371225787206E-4	1.125685612893603E-4	0.0	2.0746948688435554	0.0
72-73	2.251371225787206E-4	1.125685612893603E-4	0.0	2.3209104545237085	0.0
74-75	2.251371225787206E-4	1.125685612893603E-4	0.0	2.5584582609845805	0.0
76-77	2.251371225787206E-4	1.125685612893603E-4	0.0	2.8093172998179203	0.0
78-79	2.251371225787206E-4	1.125685612893603E-4	0.0	3.099096918717056	0.0
80-81	2.251371225787206E-4	1.125685612893603E-4	0.0	3.420930435443337	0.0
82-83	2.8142140322340076E-4	1.125685612893603E-4	0.0	3.7455218819212073	0.0
84-85	2.8142140322340076E-4	1.125685612893603E-4	0.0	4.06411905251042	0.0
86-87	2.8142140322340076E-4	1.125685612893603E-4	0.0	4.3990949487672335	0.0
88	2.8142140322340076E-4	1.125685612893603E-4	0.0	4.669794196527823	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2535	0.0	53.748375	1
GTATCAA	5035	0.0	46.656895	1
ATCAACG	6110	0.0	37.561222	3
TCAACGC	6100	0.0	37.5457	4
CAACGCA	6360	0.0	36.006756	5
TATCAAC	6510	0.0	35.470036	2
AACGCAG	6605	0.0	34.81354	6
ACGCAGA	7725	0.0	29.522654	7
CGCAGAG	7840	0.0	29.029627	8
GTACATG	5735	0.0	26.625343	1
GCAGAGT	8605	0.0	26.205156	9
TACATGG	5645	0.0	25.326242	2
ACATGGG	5820	0.0	24.079882	3
GTGGTAT	1315	0.0	21.794624	1
AGAGTAC	7915	0.0	21.396896	10-11
GAGTACT	5765	0.0	21.350025	12-13
ATGTCGC	180	5.6434183E-6	20.899199	6
GTTCGGA	135	4.0879406E-4	20.881554	1
CAGAGTA	8400	0.0	20.860558	10-11
CTGTCGC	410	0.0	20.624577	9
>>END_MODULE