FastQCFastQC Report
Wed 25 May 2016
SRR1032982_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032982_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941301
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16000.037.3079721
GTATCAA25300.033.9976161
ATCAACG26300.031.2752063
TCAACGC26200.031.215184
CAACGCA27100.030.00435
GTACATG62550.029.4561941
AACGCAG28000.029.0391276
TACATGG64050.027.0785312
TATCAAC31350.026.8369622
ACATGGG65400.025.7290153
ACGCAGA34650.023.330327
GAGTACA44950.022.3770051
GTCGTAC1903.6408346E-722.2641851
TACACCG3600.022.1948955
CGCAGAG37350.021.643798
AGTACAT45600.020.099612
CATGGGG46950.019.2213334
GTATTAG9400.019.0008751
CCGGTCG2001.4083269E-518.7998989
GTATAGA9100.018.5942631
Produced by FastQC (version 0.11.5)