FastQCFastQC Report
Wed 25 May 2016
SRR1032982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032982_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941301
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49950.2573016755258458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41470.21361962931044695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23390.12048621002101167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30400.050.378611
GTATCAA52100.041.2980841
ATCAACG63000.033.7497833
TCAACGC63100.033.6217424
CAACGCA65200.032.5363165
TATCAAC66550.031.948632
AACGCAG66800.031.7570046
GTGGTAT14950.028.5958061
GTACATG64650.028.049231
ACGCAGA78000.027.1970237
TGGTATC15550.026.317872
TACATGG65500.026.2128012
CGCAGAG81000.025.8993788
ACATGGG65500.025.6389353
GCAGAGT88450.023.6342589
GAGTACA45050.022.3162441
TACACCG3850.021.9913185
TAGCGCG1503.2709933E-521.9522914
CATGGGG47100.021.2731484
CAGAGTA85050.020.5464710-11
Produced by FastQC (version 0.11.5)