##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032982_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1941301 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.82280851861715 34.0 31.0 34.0 31.0 34.0 2 32.86652559288848 34.0 31.0 34.0 31.0 34.0 3 32.94165201583886 34.0 31.0 34.0 31.0 34.0 4 36.36191347967162 37.0 37.0 37.0 35.0 37.0 5 36.34019556987814 37.0 37.0 37.0 35.0 37.0 6 36.40421037232248 37.0 37.0 37.0 35.0 37.0 7 36.371887203478494 37.0 37.0 37.0 35.0 37.0 8 36.308766646697244 37.0 37.0 37.0 35.0 37.0 9 38.14492394533357 39.0 39.0 39.0 37.0 39.0 10-11 38.15924629926013 39.0 39.0 39.0 37.0 39.0 12-13 38.149512363100825 39.0 39.0 39.0 37.0 39.0 14-15 39.704220520156326 41.0 40.0 41.0 37.0 41.0 16-17 39.71833759937279 41.0 40.0 41.0 37.0 41.0 18-19 39.73898329007197 41.0 40.0 41.0 37.0 41.0 20-21 39.74756593645189 41.0 40.0 41.0 37.0 41.0 22-23 39.711852772959986 41.0 40.0 41.0 37.0 41.0 24-25 39.69229527002768 41.0 40.0 41.0 37.0 41.0 26-27 39.617913450824986 41.0 40.0 41.0 37.0 41.0 28-29 39.55490982593632 41.0 40.0 41.0 37.0 41.0 30-31 39.443002398906714 41.0 39.5 41.0 36.0 41.0 32-33 39.32031714813932 41.0 39.0 41.0 35.5 41.0 34-35 39.228255690384955 41.0 39.0 41.0 35.0 41.0 36-37 39.14022014102913 41.0 39.0 41.0 35.0 41.0 38-39 39.04513854368797 41.0 39.0 41.0 35.0 41.0 40-41 38.943150495466696 41.0 39.0 41.0 35.0 41.0 42-43 38.804489618044805 41.0 39.0 41.0 35.0 41.0 44-45 38.63119784103547 41.0 38.5 41.0 35.0 41.0 46-47 38.40044614410645 41.0 38.0 41.0 35.0 41.0 48-49 38.192247364010015 40.5 38.0 41.0 34.0 41.0 50-51 38.02811387827029 40.0 38.0 41.0 34.0 41.0 52-53 37.87818735991998 40.0 37.5 41.0 34.0 41.0 54-55 37.717087149288034 40.0 37.0 41.0 33.5 41.0 56-57 37.49725982730138 40.0 37.0 41.0 33.0 41.0 58-59 37.31391886162939 40.0 36.0 41.0 33.0 41.0 60-61 37.107384171748734 39.5 36.0 41.0 33.0 41.0 62-63 36.8548475996252 39.0 35.0 41.0 33.0 41.0 64-65 36.55534381324689 39.0 35.0 41.0 32.0 41.0 66-67 36.266233314668874 38.0 35.0 40.5 32.0 41.0 68-69 35.964526366596424 37.0 35.0 40.0 32.0 41.0 70-71 35.63786270135337 37.0 35.0 39.5 31.0 41.0 72-73 35.29674275138167 36.0 35.0 39.0 31.0 41.0 74-75 34.956321302054654 36.0 35.0 39.0 31.0 41.0 76-77 34.61797938598909 35.0 35.0 38.0 31.0 40.0 78-79 34.28600304641063 35.0 35.0 37.0 30.5 39.0 80-81 33.97222738771576 35.0 34.0 37.0 30.0 39.0 82-83 33.68201788388303 35.0 34.0 36.0 30.0 38.5 84-85 33.42523750824833 35.0 34.0 36.0 30.0 37.0 86-87 33.297004174004954 35.0 34.0 36.0 30.0 37.0 88-89 33.123077771041174 35.0 34.0 35.0 30.0 36.5 90-91 32.96399579457282 35.0 34.0 35.0 30.0 36.0 92-93 32.81224575684038 35.0 34.0 35.0 29.5 36.0 94-95 32.72031101822952 35.0 34.0 35.0 29.5 36.0 96-97 32.660261597763565 35.0 34.0 35.0 30.0 36.0 98-99 32.55402897335344 35.0 34.0 35.0 30.0 35.5 100 32.47364319082924 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14955780794146278 1101 2 0.21197089249737644 1101 3 0.144758470651567 1101 4 0.11542285725697354 1101 5 0.10538780160582206 1101 6 0.0902885877608881 1101 7 0.041231942280099076 1101 8 0.037807759489716375 1101 9 0.11808834844065785 1101 10-11 0.12717158140900864 1101 12-13 0.13259928031902746 1101 14-15 0.2014938830500128 1101 16-17 0.15856490728602068 1101 18-19 0.21782522069338484 1101 20-21 0.21624491502429066 1101 22-23 0.21742872898281007 1101 24-25 0.20602983552846155 1101 26-27 0.19700352542957233 1101 28-29 0.18992283665413368 1101 30-31 0.2784799221682732 1101 32-33 0.2443585986483754 1101 34-35 0.2116584094065388 1101 36-37 0.2134205659945323 1101 38-39 0.15906311601122525 1101 40-41 0.2833445143384523 1101 42-43 0.32692987286937125 1101 44-45 0.2973816021947471 1101 46-47 0.2729531894465538 1101 48-49 0.22547215536975074 1101 50-51 0.22033470343427553 1101 52-53 0.2899220474148265 1101 54-55 0.2501583662098952 1101 56-57 0.2987617188894589 1101 58-59 0.2698709542413624 1101 60-61 0.2017516413410334 1101 62-63 0.30182354446438353 1101 64-65 0.2921039401698664 1101 66-67 0.30441973268831646 1101 68-69 0.27271718294208114 1101 70-71 0.23610195043686133 1101 72-73 0.26563576255414034 1101 74-75 0.27140410152981076 1101 76-77 0.2643173732933377 1101 78-79 0.23321645458057105 1101 80-81 0.2457991689146013 1101 82-83 0.27438231960844917 1101 84-85 0.330442263121526 1101 86-87 0.2790490334420781 1101 88-89 0.23232626139272128 1101 90-91 0.25063092736869663 1101 92-93 0.2779484099341687 1101 94-95 0.33177718916922316 1101 96-97 0.3539260887943598 1101 98-99 0.3470504944027084 1101 100 0.3016047984737398 1102 1 0.15987685959904496 1102 2 0.2216669901436248 1102 3 0.1768877740623367 1102 4 0.11299751060479224 1102 5 0.10525171803106304 1102 6 0.08919034185243646 1102 7 0.12562319913913456 1102 8 -0.01162331253580362 1102 9 0.04950637216694531 1102 10-11 0.12490411743363694 1102 12-13 0.12808901036895293 1102 14-15 0.26397404050671724 1102 16-17 0.2321563886829523 1102 18-19 0.2539238189610842 1102 20-21 0.24404613180013968 1102 22-23 0.23400319378627188 1102 24-25 0.20758636288719146 1102 26-27 0.21039953820941548 1102 28-29 0.23277665502057943 1102 30-31 0.26156246100176617 1102 32-33 0.24437875388372277 1102 34-35 0.26733789027628774 1102 36-37 0.24832621733668958 1102 38-39 0.31818319561440234 1102 40-41 0.24310644527289327 1102 42-43 0.2845188979005968 1102 44-45 0.3036859025071834 1102 46-47 0.2965557562229151 1102 48-49 0.34017995949116653 1102 50-51 0.32078146352308323 1102 52-53 0.3785250337345616 1102 54-55 0.3622758652282272 1102 56-57 0.4278683659574156 1102 58-59 0.30978582481687766 1102 60-61 0.382183094886571 1102 62-63 0.37954434756903055 1102 64-65 0.41993458930709693 1102 66-67 0.40649034719857013 1102 68-69 0.3421298836850113 1102 70-71 0.4405223509085232 1102 72-73 0.4600860399459208 1102 74-75 0.4930578714665188 1102 76-77 0.45558948455138193 1102 78-79 0.4229549096689169 1102 80-81 0.32928865767348725 1102 82-83 0.4113636600059323 1102 84-85 0.3899679632622224 1102 86-87 0.3977220584898902 1102 88-89 0.3550256163251362 1102 90-91 0.3354604533876042 1102 92-93 0.38310784999600145 1102 94-95 0.4165278095859648 1102 96-97 0.3664723654291109 1102 98-99 0.359828770661224 1102 100 0.3709066454223162 1103 1 0.15593654583428673 1103 2 0.1971221389996174 1103 3 0.17165374064357763 1103 4 0.08769586228572024 1103 5 0.11067227436620186 1103 6 0.1150430494130461 1103 7 0.09451326288907325 1103 8 0.0563546143505107 1103 9 0.09024879650918649 1103 10-11 0.16119661830655474 1103 12-13 0.1321151740852926 1103 14-15 0.20285868836682397 1103 16-17 0.19384734038628437 1103 18-19 0.22620354898459993 1103 20-21 0.18796737877788416 1103 22-23 0.218149775270021 1103 24-25 0.18136699162450753 1103 26-27 0.18921559943834865 1103 28-29 0.20183752654787668 1103 30-31 0.26810304696096665 1103 32-33 0.20217262561085647 1103 34-35 0.21928223041064143 1103 36-37 0.20025717558410605 1103 38-39 0.2303330980021201 1103 40-41 0.28491530306639845 1103 42-43 0.24726159729208064 1103 44-45 0.27998932574483604 1103 46-47 0.2573743461480973 1103 48-49 0.18489578860268807 1103 50-51 0.18642347567575257 1103 52-53 0.24302189949077047 1103 54-55 0.2186292694704619 1103 56-57 0.22150882663471094 1103 58-59 0.23763075907955766 1103 60-61 0.23616721889040804 1103 62-63 0.26061663611051245 1103 64-65 0.21969510156847605 1103 66-67 0.25082599347096135 1103 68-69 0.28598387810277615 1103 70-71 0.2274162818179093 1103 72-73 0.19250079938488796 1103 74-75 0.14862017972809838 1103 76-77 0.09369604774243356 1103 78-79 0.12422027344761943 1103 80-81 0.20276943606833697 1103 82-83 0.15317600395254516 1103 84-85 0.09553268712595298 1103 86-87 0.04713974739546245 1103 88-89 0.048253544309446283 1103 90-91 0.14243579283460406 1103 92-93 0.0749179842156451 1103 94-95 0.0739221324816981 1103 96-97 0.0729377245962084 1103 98-99 0.04813593863121923 1103 100 0.0270661289665739 1104 1 0.10734546918244092 1104 2 0.06920356642321224 1104 3 0.11917255194638443 1104 4 0.08772551855766864 1104 5 0.06342653801546305 1104 6 0.08859571789757581 1104 7 0.05136088290075236 1104 8 0.007789107995364475 1104 9 0.03584153180336358 1104 10-11 0.08074587460632898 1104 12-13 0.10063846836197854 1104 14-15 0.08977332628087709 1104 16-17 0.10271104648709439 1104 18-19 0.07667306708205501 1104 20-21 0.06341261827402889 1104 22-23 0.14716907003596447 1104 24-25 0.1325552064065718 1104 26-27 0.13210533030174787 1104 28-29 0.16646728183222592 1104 30-31 0.20629100656728383 1104 32-33 0.17640007313638506 1104 34-35 0.18845921482382977 1104 36-37 0.20550943315132741 1104 38-39 0.16177346490687938 1104 40-41 0.14104495132455952 1104 42-43 0.09719170075425154 1104 44-45 0.08218245318720108 1104 46-47 0.04704092793192416 1104 48-49 0.08213726763082718 1104 50-51 0.10383932567459198 1104 52-53 0.17216614601584723 1104 54-55 0.12081649201955713 1104 56-57 0.1634029634974823 1104 58-59 0.17610790028875556 1104 60-61 0.12794858283639599 1104 62-63 0.03246717957158296 1104 64-65 0.06633863107079918 1104 66-67 0.16363609263099477 1104 68-69 0.15710351967575065 1104 70-71 0.21562916587321723 1104 72-73 0.15065678821198958 1104 74-75 0.10266930211333403 1104 76-77 0.11092568271458703 1104 78-79 0.21934190897652428 1104 80-81 0.1684015074416294 1104 82-83 0.15454691455035174 1104 84-85 0.11624858162238638 1104 86-87 0.12468186984607854 1104 88-89 0.09159684478626673 1104 90-91 0.03088847091458291 1104 92-93 0.05253850466871768 1104 94-95 0.11022483629015056 1104 96-97 0.05068418535081065 1104 98-99 0.018627289017032922 1104 100 0.0385765848454227 1105 1 0.10020689557121187 1105 2 0.1643953882788054 1105 3 0.15401723410331414 1105 4 0.10260386697711965 1105 5 0.050598401196737086 1105 6 0.10491704964110227 1105 7 0.1015055579256412 1105 8 0.06114069166077485 1105 9 0.11998132188018218 1105 10-11 0.1422416752385729 1105 12-13 0.11430813617947422 1105 14-15 0.1630648124281322 1105 16-17 0.16206283909126284 1105 18-19 0.13380507368772498 1105 20-21 0.14366180286037178 1105 22-23 0.209959688283746 1105 24-25 0.23437373957609253 1105 26-27 0.1919598818016368 1105 28-29 0.15777171263984968 1105 30-31 0.21904550000630252 1105 32-33 0.30144376946398665 1105 34-35 0.317220175319342 1105 36-37 0.27916229295041006 1105 38-39 0.21326468998113057 1105 40-41 0.2591818654798601 1105 42-43 0.27556318633898513 1105 44-45 0.21439787206421101 1105 46-47 0.26138208776304594 1105 48-49 0.3287769195870709 1105 50-51 0.34870519552784884 1105 52-53 0.3739487552792724 1105 54-55 0.3606299739940724 1105 56-57 0.406267089515687 1105 58-59 0.4238321754570862 1105 60-61 0.4495106110290692 1105 62-63 0.47256472534473914 1105 64-65 0.42679704572785226 1105 66-67 0.48468456341019817 1105 68-69 0.4186450633313967 1105 70-71 0.364671123890588 1105 72-73 0.4060157409868026 1105 74-75 0.42319705832198196 1105 76-77 0.3389181152138008 1105 78-79 0.44253157514662433 1105 80-81 0.4646068580820568 1105 82-83 0.48650628299276377 1105 84-85 0.46326346951367725 1105 86-87 0.48753165392594866 1105 88-89 0.3938218668102209 1105 90-91 0.35260933776936554 1105 92-93 0.35804841067641036 1105 94-95 0.42049073597744524 1105 96-97 0.40179855471235015 1105 98-99 0.38800138683855323 1105 100 0.3724221780958956 1106 1 0.09894149216941628 1106 2 0.1116256007306049 1106 3 0.14959035699251189 1106 4 0.008595836955983316 1106 5 0.049983174798214236 1106 6 -0.008855307973483661 1106 7 0.04549996678642998 1106 8 0.01031592920519131 1106 9 0.037144797827942 1106 10-11 0.03726107043032556 1106 12-13 0.08487326351936986 1106 14-15 0.07330429828080298 1106 16-17 0.11495217685869363 1106 18-19 0.13028864786105032 1106 20-21 0.152685163270057 1106 22-23 0.11335382290198481 1106 24-25 0.09882326515754158 1106 26-27 0.06856066737633171 1106 28-29 0.07803597751372138 1106 30-31 0.15026253033481396 1106 32-33 0.14653131833498634 1106 34-35 0.05180390643524646 1106 36-37 0.03411073602065784 1106 38-39 0.09209441446503774 1106 40-41 0.0969266753150535 1106 42-43 0.042771511573029386 1106 44-45 0.09989406532095302 1106 46-47 0.05567467794757874 1106 48-49 0.1339426189678221 1106 50-51 0.17922761849527546 1106 52-53 0.06737354109908722 1106 54-55 0.05021785369888221 1106 56-57 0.07489424173083137 1106 58-59 0.09090252958810652 1106 60-61 0.0854734906141772 1106 62-63 0.0600516547761174 1106 64-65 0.05246900701215651 1106 66-67 -3.379055196717218E-4 1106 68-69 0.06017352748691707 1106 70-71 0.008444206849915759 1106 72-73 0.024979819425539063 1106 74-75 0.0649704759453229 1106 76-77 0.04696368457830147 1106 78-79 -0.00917183593664106 1106 80-81 0.028034066505441047 1106 82-83 -0.0047387102134166526 1106 84-85 0.041585620249414035 1106 86-87 0.06375365633706309 1106 88-89 0.032496649996780036 1106 90-91 0.054707259788337126 1106 92-93 0.06240244210897572 1106 94-95 0.02854998634921202 1106 96-97 -0.022875530898438967 1106 98-99 0.02038278698850604 1106 100 0.020115653631911812 1107 1 0.06060440176361226 1107 2 0.029875371469174183 1107 3 0.06280379340034159 1107 4 0.04704073951447185 1107 5 0.04061531494419768 1107 6 0.09357149335674819 1107 7 0.027979382316075885 1107 8 0.06910006743275687 1107 9 0.08527714209282067 1107 10-11 0.10117685687442446 1107 12-13 0.08409031134229394 1107 14-15 0.11376737892604183 1107 16-17 0.1313423106100089 1107 18-19 0.12350781244651898 1107 20-21 0.14661591142448316 1107 22-23 0.10674749599895961 1107 24-25 0.08272246044436571 1107 26-27 0.137605065074915 1107 28-29 0.16143556918250113 1107 30-31 0.12470468420903558 1107 32-33 0.1001648506944548 1107 34-35 0.12524553316293208 1107 36-37 0.13513107350423326 1107 38-39 0.13879675842738948 1107 40-41 0.13113157003350295 1107 42-43 0.19296628012556738 1107 44-45 0.14352423015673565 1107 46-47 0.1280873001692342 1107 48-49 0.12066163351568093 1107 50-51 0.10868325356083375 1107 52-53 0.10487015225827179 1107 54-55 0.16415176262996312 1107 56-57 0.1853313224767632 1107 58-59 0.11266820296960844 1107 60-61 0.07156052504059573 1107 62-63 0.14414881326594298 1107 64-65 0.1483779037574351 1107 66-67 0.03596009848374848 1107 68-69 0.024343858073088143 1107 70-71 0.04764537136134095 1107 72-73 0.02128442064497449 1107 74-75 0.024277403828101285 1107 76-77 0.09046804910158812 1107 78-79 0.06289165245579653 1107 80-81 0.08909090360057803 1107 82-83 0.10117370590364771 1107 84-85 0.0733100178125099 1107 86-87 0.05764136139478637 1107 88-89 0.07421557285174885 1107 90-91 0.025829371710436533 1107 92-93 -0.011301881835827032 1107 94-95 0.044563887225336885 1107 96-97 0.05264425391644778 1107 98-99 0.10871679955008062 1107 100 0.13894064834350672 1108 1 0.04780162519936226 1108 2 0.02544294100463418 1108 3 0.037307458270980476 1108 4 0.04514155986299784 1108 5 -0.005869079017259082 1108 6 7.817015990667642E-4 1108 7 0.029541806470248844 1108 8 0.016532837686625612 1108 9 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-0.10517265926163333 2214 36-37 -0.029932324556767753 2214 38-39 -0.04556565544240243 2214 40-41 -0.005663032706451077 2214 42-43 -0.03424008459575134 2214 44-45 0.008338885258588391 2214 46-47 -0.046950072664301956 2214 48-49 -0.015851225573960903 2214 50-51 -0.06270637499871867 2214 52-53 -0.08862804278560077 2214 54-55 -0.10117229697011965 2214 56-57 -0.08217371746545865 2214 58-59 -0.10005009969683698 2214 60-61 -0.11938670459829837 2214 62-63 -0.12365973121791285 2214 64-65 -0.16094218617693912 2214 66-67 -0.0893734021489152 2214 68-69 -0.09707767315099858 2214 70-71 -0.06226699413539194 2214 72-73 -0.09115864667444384 2214 74-75 -0.09353730956897977 2214 76-77 -0.06095860691222299 2214 78-79 -0.04703378953985293 2214 80-81 -0.1075444533359331 2214 82-83 -0.026100956073847215 2214 84-85 -0.09822285331151193 2214 86-87 -0.030934250515485928 2214 88-89 -0.06512205955528572 2214 90-91 -0.07128529381268578 2214 92-93 -0.07192787480126839 2214 94-95 -0.1950553690472887 2214 96-97 -0.05110279189282352 2214 98-99 -0.06500408926854107 2214 100 -0.06532474588997417 2215 1 -0.1757771566861237 2215 2 -0.17898581713818373 2215 3 -0.2150921655343936 2215 4 -0.13863769779804613 2215 5 -0.15103998017857378 2215 6 -0.10671739172271089 2215 7 -0.18648551758423793 2215 8 -0.1176938618355976 2215 9 -0.15155039602147014 2215 10-11 -0.25966174674636733 2215 12-13 -0.24388805859462082 2215 14-15 -0.32133922442513096 2215 16-17 -0.28797010895155495 2215 18-19 -0.29419157002115526 2215 20-21 -0.33401594869560114 2215 22-23 -0.24221205091176046 2215 24-25 -0.2783479164099276 2215 26-27 -0.3134714887803085 2215 28-29 -0.2099086237902128 2215 30-31 -0.2330876582243917 2215 32-33 -0.26211349055331823 2215 34-35 -0.25482788028093495 2215 36-37 -0.22167915637513858 2215 38-39 -0.19585018683889643 2215 40-41 -0.28437457740979255 2215 42-43 -0.29950530877888326 2215 44-45 -0.2862559311795394 2215 46-47 -0.11817400562250668 2215 48-49 -0.0988817993671347 2215 50-51 -0.1044497767747643 2215 52-53 -0.1650994285534182 2215 54-55 -0.24614474229678507 2215 56-57 -0.16966232238703327 2215 58-59 -0.1979435076333047 2215 60-61 -0.16699176713599684 2215 62-63 -0.08892155095605148 2215 64-65 -0.1724051828462123 2215 66-67 -0.21137510147038086 2215 68-69 -0.21382270462377306 2215 70-71 -0.19648941377940332 2215 72-73 -0.16433480272669243 2215 74-75 -0.19103647594923956 2215 76-77 -0.10282184685852513 2215 78-79 -0.1782852976492535 2215 80-81 -0.15388999921266588 2215 82-83 -0.22662174230097776 2215 84-85 -0.26204956723198336 2215 86-87 -0.28017392446692924 2215 88-89 -0.2635697606248826 2215 90-91 -0.2581046821183932 2215 92-93 -0.298371461867859 2215 94-95 -0.4034361569877376 2215 96-97 -0.3137639142914068 2215 98-99 -0.35200639609998063 2215 100 -0.3678253832635079 2216 1 -0.221713003047185 2216 2 -0.2272882682755224 2216 3 -0.1785408387786518 2216 4 -0.12399036802866448 2216 5 -0.15390613959603172 2216 6 -0.11534858131374648 2216 7 -0.11085706411494556 2216 8 -0.08930308523773078 2216 9 -0.1692614455401369 2216 10-11 -0.14873351849262662 2216 12-13 -0.21651557385426656 2216 14-15 -0.31334332133856435 2216 16-17 -0.3369940672206013 2216 18-19 -0.24840639773982787 2216 20-21 -0.22790161796313413 2216 22-23 -0.26100774380575587 2216 24-25 -0.2805792335509594 2216 26-27 -0.2876538022360222 2216 28-29 -0.330095493571946 2216 30-31 -0.3817452656540894 2216 32-33 -0.38030916865048425 2216 34-35 -0.397823195371096 2216 36-37 -0.4098052150513638 2216 38-39 -0.3431505160199606 2216 40-41 -0.23360104713428598 2216 42-43 -0.2541152167768246 2216 44-45 -0.12499424111632607 2216 46-47 -0.15916527064509012 2216 48-49 -0.2395282384188846 2216 50-51 -0.2300342193590197 2216 52-53 -0.09282336230108257 2216 54-55 -0.08819780121886822 2216 56-57 -0.0878378971454481 2216 58-59 -0.11022183379491679 2216 60-61 -0.1465187902555556 2216 62-63 -0.14557237995021666 2216 64-65 -0.12316290667980923 2216 66-67 -0.14032357004666096 2216 68-69 -0.10337743303566072 2216 70-71 -0.19850124187638585 2216 72-73 -0.11844350032433937 2216 74-75 -0.1459167976801936 2216 76-77 -0.15661293754986616 2216 78-79 -0.15513536211885537 2216 80-81 -0.18783310151403043 2216 82-83 -0.18376879826433878 2216 84-85 -0.18367130171154145 2216 86-87 -0.16576270594775622 2216 88-89 -0.23574642479202623 2216 90-91 -0.2839901336334023 2216 92-93 -0.22194065176065436 2216 94-95 -0.27087943391111224 2216 96-97 -0.1681431710057879 2216 98-99 -0.17463513904443317 2216 100 -0.15939551782745554 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 4.0 4 7.0 5 14.0 6 47.0 7 115.0 8 227.0 9 419.0 10 737.0 11 1097.0 12 1764.0 13 2897.0 14 3368.0 15 4335.0 16 5567.0 17 8436.0 18 10354.0 19 5753.0 20 4007.0 21 3831.0 22 3963.0 23 4208.0 24 4677.0 25 5429.0 26 6922.0 27 8764.0 28 11626.0 29 14420.0 30 18446.0 31 23565.0 32 30223.0 33 40205.0 34 57009.0 35 91303.0 36 177316.0 37 401889.0 38 675113.0 39 312126.0 40 1112.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.60623212534307 18.714920341874706 12.78625207078158 23.892595462000642 2 16.213273057138412 19.281909002589007 39.769668147815416 24.735149792457165 3 19.23447016838236 24.218717805012734 29.288990447618495 27.25782157898641 4 12.683974766819759 15.743588026345954 37.33310322266556 34.23933398416873 5 12.982057779194935 37.608607073554815 35.047316548248666 14.36201859900159 6 32.34853975775015 36.76514602422498 16.885575506936622 14.000738711088239 7 28.84921188881397 31.97172803403103 21.107852677115748 18.071207400039253 8 26.406068249755627 35.827072420258794 19.067014337555417 18.69984499243016 9 25.74336683890795 16.071600587038684 20.20173402037533 37.98329855367803 10-11 24.957850431231428 26.14419402246226 27.686407208361814 21.211548337944503 12-13 25.62628860336965 23.32177319550455 28.40561531656019 22.646322884565606 14-15 22.51305569778571 25.187941264040862 25.815305017570715 26.48369802060271 16-17 21.950717585783966 28.824509954921986 26.341484396288877 22.883288063005168 18-19 21.82901993590366 27.20249070659737 29.15167775762229 21.816811599876683 20-21 22.656841983803645 26.527390651939086 29.092629118307773 21.723138245949496 22-23 22.424349506721285 26.282725560656385 29.12925179879833 22.163673133824002 24-25 22.146313420730475 26.38593946349634 29.185905362486775 22.281841753286407 26-27 21.903641574619968 26.51709952634468 29.196063527355403 22.38319537167995 28-29 22.085475862307007 26.526026269553864 29.233526921875214 22.15497094626392 30-31 22.206442967611544 26.785927263332006 28.924266704722346 22.083363064334108 32-33 21.954313903540125 26.607108586590417 29.244408379863085 22.194169130006376 34-35 22.109129993345512 26.36604423289316 29.258414912854036 22.266410860907293 36-37 22.380359050385472 26.708694493870254 28.729389952381172 22.181556503363105 38-39 22.23026989951397 26.945246850261928 28.790758758548296 22.03372449167581 40-41 22.11910601044625 26.661464758143467 28.972843821372162 22.24658541003812 42-43 22.112919779028537 26.690288915498574 28.778868224862226 22.417923080610667 44-45 22.15704388824415 26.85143095678935 28.670806325979736 22.32071882898676 46-47 22.265676873596128 27.068935774758728 28.349944057902327 22.315443293742813 48-49 22.299428217805826 27.07037368899432 28.126037040140783 22.504161053059065 50-51 22.37495116916805 27.034527749449804 28.03878618477458 22.551734896607563 52-53 22.366840440582497 27.10200130929909 28.04380453747682 22.487353712641596 54-55 22.360347746263326 27.425050572249667 27.882257230639112 22.3323444508479 56-57 22.196896572349033 27.171930139362406 28.023918688421805 22.607254599866764 58-59 22.161225749820513 26.770110038552808 28.35362371893444 22.71504049269224 60-61 22.387604133502276 27.104602046643045 28.032822596341617 22.474971223513066 62-63 22.14188268801049 27.057569142469422 28.131454474397614 22.669093695122466 64-65 22.15833608492449 26.91285380268181 28.199568227698848 22.72924188469485 66-67 22.072883615905937 26.95376115135189 28.07383503996419 22.899520192777985 68-69 22.36650697985886 27.076598155874933 28.009581208468553 22.54731365579766 70-71 22.34166674822709 26.922821345067042 28.03400915159473 22.701502755111132 72-73 22.22823766123852 27.01229742322288 28.030197274920273 22.72926764061833 74-75 22.21229474460684 27.111972847075233 27.889054814271457 22.786677594046466 76-77 22.223664554679853 27.049966517282233 27.79699170658837 22.929377221449542 78-79 22.237406769996 27.182930416251782 27.848927085495756 22.730735728256462 80-81 22.152400649565564 27.119542470950485 27.876871010239284 22.851185869244667 82-83 22.107316314312303 27.097275354614446 27.987529996031007 22.807878335042243 84-85 22.16423419140051 27.115578676361885 27.75339836532305 22.966788766914558 86-87 22.236682891348547 27.056000651116452 27.836902540126857 22.870413917408143 88-89 21.953469084989877 27.075810414724227 27.842697381637983 23.12802311864791 90-91 21.938828993319152 27.006398562197255 27.880144985655196 23.174627458828397 92-93 22.12425690003459 27.126635489586366 27.740432648311565 23.008674962067484 94-95 21.915324678535846 26.9361254516364 27.892997073056147 23.255552796771607 96-97 21.97363100404627 26.871289525806226 27.854035579342657 23.30104389080485 98-99 21.86572867003331 27.016528353029322 27.798761706392185 23.318981270545184 100 21.981186843256147 26.84102053210708 27.689111580326802 23.488681044309974 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 61.0 1 62.5 2 267.5 3 530.0 4 351.5 5 168.5 6 531.5 7 1217.5 8 2004.0 9 2280.0 10 1632.5 11 1007.0 12 937.0 13 1030.5 14 1185.0 15 1330.0 16 1438.5 17 1604.0 18 1791.5 19 2039.5 20 2372.5 21 2835.0 22 3520.5 23 4598.0 24 6056.0 25 7892.5 26 10308.0 27 13312.5 28 17250.0 29 21885.5 30 26043.5 31 29866.5 32 34031.0 33 38476.5 34 42821.0 35 46568.0 36 50473.5 37 54775.0 38 58114.0 39 60123.5 40 62146.0 41 64438.5 42 66392.5 43 67866.5 44 67686.5 45 67793.0 46 68722.5 47 67589.0 48 66055.0 49 65333.5 50 64648.5 51 63621.5 52 60432.0 53 57631.0 54 56216.5 55 53654.0 56 49660.5 57 45062.0 58 41113.0 59 38482.0 60 35006.5 61 30079.5 62 26376.5 63 22642.5 64 18453.0 65 14981.5 66 11791.5 67 8901.0 68 6745.0 69 5218.0 70 3880.0 71 2856.5 72 2182.5 73 1581.0 74 1147.0 75 800.0 76 489.0 77 315.0 78 235.5 79 141.5 80 77.5 81 37.0 82 20.5 83 14.0 84 8.5 85 7.5 86 3.5 87 1.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.5755923476060644E-4 2 7.726777042818192E-4 3 2.5755923476060644E-4 4 0.010611440472136984 5 7.21165857329698E-4 6 0.004223971450073945 7 7.21165857329698E-4 8 0.13836082091339777 9 0.0016483791024678811 10-11 0.0 12-13 1.802914643324245E-4 14-15 1.5453554085636384E-4 16-17 0.0 18-19 2.318033112845458E-4 20-21 0.0 22-23 2.5755923476060644E-4 24-25 4.893625460451522E-4 26-27 0.0062586894046827365 28-29 0.00806160404800698 30-31 0.014706632304830627 32-33 0.017436760193293054 34-35 0.02603923863429731 36-37 0.03513107962134671 38-39 0.0403080202400349 40-41 0.05086794886521977 42-43 0.06809866167070433 44-45 0.10773702790036167 46-47 0.11551531679013197 48-49 0.11273367705471743 50-51 0.11376391399375986 52-53 0.10794307528817015 54-55 0.1155668286370841 56-57 0.09771797366817407 58-59 0.09032602363054466 60-61 0.09187137903910832 62-63 0.09040329140097285 64-65 0.0 66-67 2.5755923476060643E-5 68-69 5.1511846952121285E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 5.1511846952121285E-5 78-79 0.0 80-81 1.802914643324245E-4 82-83 1.2877961738030322E-4 84-85 0.0 86-87 0.0010302369390424258 88-89 5.666303164733341E-4 90-91 2.8331515823666706E-4 92-93 0.0012620402503269715 94-95 0.0013908198677072747 96-97 3.090710817127277E-4 98-99 2.5755923476060643E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1941301.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.18626083968265 #Duplication Level Percentage of deduplicated Percentage of total 1 79.35424293185272 50.93452135559442 2 12.63008638143226 16.21356037812669 3 3.522600020046196 6.783075711616695 4 1.4932427973770348 3.83382686757679 5 0.7892282857385613 2.5328806305235445 6 0.4927276464191561 1.8975807141589731 7 0.3420743008540958 1.536952921082115 8 0.22632662644764848 1.1621647904107346 9 0.17926145209219727 1.0355510090241045 >10 0.915535714956962 10.339436125570401 >50 0.0434365703259671 1.8778898493248601 >100 0.010675820676738956 1.082644405205508 >500 2.4062219157047085E-4 0.1138784697311061 >1k 3.2082958876062767E-4 0.6560367720540164 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4995 0.2573016755258458 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4147 0.21361962931044695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2339 0.12048621002101167 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.1511846952121285E-5 0.0 5 0.0 0.0 0.0 5.1511846952121285E-5 0.0 6 0.0 0.0 0.0 5.1511846952121285E-5 0.0 7 0.0 0.0 0.0 5.1511846952121285E-5 0.0 8 0.0 0.0 0.0 5.1511846952121285E-5 0.0 9 5.1511846952121285E-5 0.0 0.0 5.1511846952121285E-5 0.0 10-11 5.1511846952121285E-5 0.0 0.0 5.1511846952121285E-5 0.0 12-13 5.1511846952121285E-5 0.0 0.0 5.1511846952121285E-5 0.0 14-15 5.1511846952121285E-5 0.0 0.0 5.1511846952121285E-5 0.0 16-17 5.1511846952121285E-5 0.0 0.0 7.726777042818192E-5 0.0 18-19 5.1511846952121285E-5 0.0 0.0 1.0302369390424257E-4 0.0 20-21 1.0302369390424257E-4 0.0 0.0 1.0302369390424257E-4 0.0 22-23 1.0302369390424257E-4 0.0 0.0 2.0604738780848514E-4 0.0 24-25 1.0302369390424257E-4 0.0 0.0 3.8633885214090966E-4 0.0 26-27 1.0302369390424257E-4 0.0 0.0 5.151184695212129E-4 0.0 28-29 1.0302369390424257E-4 0.0 0.0 0.0013393080207551534 0.0 30-31 1.0302369390424257E-4 0.0 0.0 0.004326995143978187 0.0 32-33 1.0302369390424257E-4 0.0 0.0 0.011281094482514561 0.0 34-35 1.0302369390424257E-4 0.0 0.0 0.022381897500696697 0.0 36-37 1.0302369390424257E-4 0.0 0.0 0.04272907704678461 0.0 38-39 1.2877961738030322E-4 0.0 0.0 0.07749957373946648 0.0 40-41 1.5453554085636384E-4 0.0 0.0 0.12458140185370532 0.0 42-43 1.5453554085636384E-4 0.0 0.0 0.17246166359570206 0.0 44-45 1.5453554085636384E-4 0.0 0.0 0.2256991574207194 0.0 46-47 1.5453554085636384E-4 0.0 0.0 0.29111920304991346 0.0 48-49 1.5453554085636384E-4 0.0 0.0 0.36864453271285597 0.0 50-51 1.5453554085636384E-4 0.0 0.0 0.45482385266375486 0.0 52-53 2.0604738780848514E-4 0.0 0.0 0.5429091109518822 0.0 54-55 2.0604738780848514E-4 0.0 0.0 0.6357077032361287 0.0 56-57 2.318033112845458E-4 0.0 0.0 0.7481323092091334 0.0 58-59 2.5755923476060644E-4 0.0 0.0 0.8805692677230372 0.0 60-61 2.5755923476060644E-4 0.0 0.0 1.0217374842953257 0.0 62-63 2.5755923476060644E-4 0.0 0.0 1.1648889069752708 0.0 64-65 2.5755923476060644E-4 0.0 0.0 1.31749275357093 0.0 66-67 2.5755923476060644E-4 0.0 0.0 1.4870439978138372 0.0 68-69 2.5755923476060644E-4 0.0 0.0 1.6827890162318981 0.0 70-71 2.5755923476060644E-4 0.0 0.0 1.9007098847628472 0.0 72-73 2.5755923476060644E-4 0.0 0.0 2.131045108409258 0.0 74-75 2.5755923476060644E-4 0.0 0.0 2.362462080841662 0.0 76-77 2.5755923476060644E-4 0.0 0.0 2.6017346099342658 0.0 78-79 2.5755923476060644E-4 0.0 0.0 2.870574939177387 0.0 80-81 2.5755923476060644E-4 0.0 0.0 3.166355964376467 0.0 82-83 2.5755923476060644E-4 5.1511846952121285E-5 0.0 3.47931619053408 0.0 84-85 2.5755923476060644E-4 1.0302369390424257E-4 0.0 3.7838027178680687 0.0 86-87 2.5755923476060644E-4 1.0302369390424257E-4 0.0 4.100446041082758 0.0 88 2.5755923476060644E-4 1.0302369390424257E-4 0.0 4.364238209324571 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3040 0.0 50.37861 1 GTATCAA 5210 0.0 41.298084 1 ATCAACG 6300 0.0 33.749783 3 TCAACGC 6310 0.0 33.621742 4 CAACGCA 6520 0.0 32.536316 5 TATCAAC 6655 0.0 31.94863 2 AACGCAG 6680 0.0 31.757004 6 GTGGTAT 1495 0.0 28.595806 1 GTACATG 6465 0.0 28.04923 1 ACGCAGA 7800 0.0 27.197023 7 TGGTATC 1555 0.0 26.31787 2 TACATGG 6550 0.0 26.212801 2 CGCAGAG 8100 0.0 25.899378 8 ACATGGG 6550 0.0 25.638935 3 GCAGAGT 8845 0.0 23.634258 9 GAGTACA 4505 0.0 22.316244 1 TACACCG 385 0.0 21.991318 5 TAGCGCG 150 3.2709933E-5 21.952291 4 CATGGGG 4710 0.0 21.273148 4 CAGAGTA 8505 0.0 20.54647 10-11 >>END_MODULE