FastQCFastQC Report
Wed 25 May 2016
SRR1032981_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032981_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3993668
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37200.033.861821
GTATCAA58700.033.149781
TCAACGC59000.031.4675924
CAACGCA60150.030.9433335
ATCAACG61450.030.2894733
AACGCAG64050.029.0588326
TATCAAC71850.026.4939482
GTACATG119350.025.6769581
TACATGG121000.024.2780082
ACGCAGA79650.023.0134077
GTCGTAC2704.7293724E-1122.6307011
CGCAGAG80850.022.381178
ACATGGG124450.022.3586313
CGTACAC4450.017.9559063
GAGTACA90300.017.7494561
GCAGAGT98500.017.4164129
GTATAAG19200.017.381031
CATGGGG84500.017.1877964
GTATAGG16600.016.9887561
TACACCG6250.016.5443675
Produced by FastQC (version 0.11.5)